Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 295,026 T→C S40P (TCG→CCG)  rimI2 → Ribosomal‑protein‑alanine acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM406671295,0260TC99.8% 5003.0 / NA 1234S40P (TCG→CCG) rimI2Ribosomal‑protein‑alanine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (703/527);  total (705/529)
Fisher's exact test for biased strand distribution p-value = 1.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1241 total aligned reads displayed.

GCAATTTGGATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGTCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCAATATCGCCATTCA  >  AM406671/294880‑295171
                                                                                                                                                  |                                                                                                                                                 
  aaTTTGGATGGTCAAATAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAgccgcc                                                                                                                                               <  1:6479263/149‑1 (MQ=255)
    tttGGATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCtt                                                                                                                                             <  1:504393/149‑1 (MQ=255)
       ggATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGt                                                                                                                                          <  2:8341627/149‑1 (MQ=255)
       agATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGt                                                                                                                                          <  2:7785367/148‑1 (MQ=255)
        gATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGta                                                                                                                                         >  1:840070/1‑148 (MQ=255)
        gATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTc                                                                                                                                         <  1:3737049/149‑1 (MQ=255)
          tGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTcac                                                                                                                                       >  1:922454/1‑149 (MQ=255)
          tGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTcac                                                                                                                                       >  1:3974909/1‑149 (MQ=255)
          tGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTcac                                                                                                                                       <  2:1641762/149‑1 (MQ=255)
           ggTCAAATAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTcaca                                                                                                                                      <  2:5982328/149‑1 (MQ=255)
            gTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACaa                                                                                                                                     <  1:407257/149‑1 (MQ=255)
            gTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACaa                                                                                                                                     >  2:4339098/1‑149 (MQ=255)
            gTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACaa                                                                                                                                     >  2:3861797/1‑149 (MQ=255)
              cAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGt                                                                                                                                   >  1:5606465/1‑149 (MQ=255)
               aaaGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGtt                                                                                                                                  <  1:6416383/149‑1 (MQ=255)
                aaGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGttt                                                                                                                                 >  1:7051428/1‑149 (MQ=255)
                aaGAATTAAAAAAATGAATTTAAAACGTCTGGCTTACTCTGAAAATAAGTCGAAAAACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGttt                                                                                                                                 <  2:7139443/149‑1 (MQ=255)
                 aGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTta                                                                                                                                >  1:8681937/1‑149 (MQ=255)
                  aaaTTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTtat                                                                                                                               <  2:7954417/148‑1 (MQ=255)
                   aaTTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTtata                                                                                                                              >  1:3086141/1‑149 (MQ=255)
                   aaTTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGCCACAAGTTtata                                                                                                                              >  2:7265405/1‑149 (MQ=255)
                     ttAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTtatata                                                                                                                            >  2:1504157/1‑149 (MQ=255)
                      tAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTatatat                                                                                                                           >  1:3317232/1‑149 (MQ=255)
                          aaaaTGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAg                                                                                                                       >  1:1548925/1‑149 (MQ=255)
                           aaaTGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGt                                                                                                                      >  2:177377/1‑149 (MQ=255)
                               gAATTTAAAGCGTCTGTCTGACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTACCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGAtt                                                                                                                  <  1:4748765/149‑1 (MQ=255)
                                aaTTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGAttt                                                                                                                 >  1:7483918/1‑149 (MQ=255)
                                 aTTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTa                                                                                                                <  2:2922494/149‑1 (MQ=255)
                                   ttAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGt                                                                                                              <  2:2575218/149‑1 (MQ=255)
                                     aaaaCGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTaa                                                                                                            <  1:1000322/149‑1 (MQ=255)
                                       aaCGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAAc                                                                                                          <  1:6247173/149‑1 (MQ=255)
                                         cGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGGGTGATTTAGTGGACGa                                                                                                        <  2:124765/149‑1 (MQ=255)
                                         cGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGa                                                                                                        <  1:5210452/149‑1 (MQ=255)
                                          gtctgtctTACTCTGAAAATAAGTCGAANGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGaa                                                                                                       >  1:5330129/1‑149 (MQ=255)
                                            ctgtctTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGaaaa                                                                                                     >  2:3583639/1‑149 (MQ=255)
                                            ctgtctTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGaaaa                                                                                                     >  1:8075769/1‑149 (MQ=255)
                                              gtctTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAAtt                                                                                                   >  1:4889344/1‑149 (MQ=255)
                                                   aCTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGaa                                                                                              <  2:243116/149‑1 (MQ=255)
                                                    ctctGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAAt                                                                                             >  1:956548/1‑149 (MQ=255)
                                                     tctGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATa                                                                                            <  2:487595/149‑1 (MQ=255)
                                                     tctGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATa                                                                                            >  1:1314847/1‑149 (MQ=255)
                                                        gAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACtt                                                                                         <  1:4528936/149‑1 (MQ=255)
                                                           aaTAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACttttt                                                                                      <  1:1691708/149‑1 (MQ=255)
                                                            aTAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTa                                                                                     <  2:8939378/149‑1 (MQ=255)
                                                             tAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCAGACATGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTag                                                                                    <  2:9092746/149‑2 (MQ=255)
                                                              aaGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACt                                                                                   <  1:2865049/149‑1 (MQ=255)
                                                               aGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTa                                                                                  >  1:2455239/1‑149 (MQ=255)
                                                                gTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGGCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCAGCCTTGGTCACAAGTTTATATACCGAGTGATTTAGTAAACGAAAATTCGGCATACTTTTTACTag                                                                                 >  2:4180798/1‑149 (MQ=255)
                                                                 tCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTaga                                                                                <  1:2285376/149‑1 (MQ=255)
                                                                   gAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTagaag                                                                              <  2:9265532/149‑1 (MQ=255)
                                                                    aaaTACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTagaaga                                                                             <  2:8506315/149‑1 (MQ=255)
                                                                    aaaGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTagaaga                                                                             >  1:2117605/1‑149 (MQ=255)
                                                                     aaGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTagaagaa                                                                            >  2:2295637/1‑149 (MQ=255)
                                                                      aGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAAGTCGGAATACTTTTTACTagaagaag                                                                           <  1:386729/149‑1 (MQ=255)
                                                                      aGACAAAAATACGTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGGAAACGAAAATTCGGAATACTTTTTACTagaagaag                                                                           <  2:8687592/149‑1 (MQ=255)
                                                                       aaaaaaaaTACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTagaagaaga                                                                          <  2:8038795/146‑1 (MQ=255)
                                                                         cAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATg                                                                        >  2:5780303/1‑149 (MQ=255)
                                                                          aaaaaTACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGa                                                                       <  2:4387616/149‑1 (MQ=255)
                                                                          aaaaaTACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGa                                                                       <  1:4330075/149‑1 (MQ=255)
                                                                          aaaaaTACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGa                                                                       <  2:5435721/149‑1 (MQ=255)
                                                                                  ttttCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCaattaa                                                               >  2:4723884/1‑149 (MQ=255)
                                                                                  ttttCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCaattaa                                                               <  1:2559112/149‑1 (MQ=255)
                                                                                   tttCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCaattaat                                                              >  2:2101184/1‑149 (MQ=255)
                                                                                    ttCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCaattaatt                                                             <  2:3315072/149‑1 (MQ=255)
                                                                                       cACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGt                                                          <  1:3581421/149‑1 (MQ=255)
                                                                                       cACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGt                                                          >  1:6576442/1‑149 (MQ=255)
                                                                                       cACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCATTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGt                                                          <  2:8193923/149‑1 (MQ=255)
                                                                                        aCCTGTGGAAAAGTTATCAGTCAATATTTATGAACTCTTATCAGCGGCTTATGGACAACCGCCTTGGTCAAAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTAATAGAAAAAGACGAGCAATGAATTGGta                                                         >  2:6449535/1‑148 (MQ=255)
                                                                                         ccTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTct                                                        >  1:7617588/1‑149 (MQ=255)
                                                                                            gtgGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTctgct                                                     <  2:4005511/149‑1 (MQ=255)
                                                                                             tgGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTctgctg                                                    <  1:7218346/149‑1 (MQ=255)
                                                                                                aaaaGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTTGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGcc                                                 <  1:5762791/149‑1 (MQ=255)
                                                                                                aaaaGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGcc                                                 >  1:3444394/1‑149 (MQ=255)
                                                                                                  aaGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCAt                                               >  1:9372595/1‑149 (MQ=255)
                                                                                                    gTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCAttt                                             <  1:6869907/149‑1 (MQ=255)
                                                                                                     ttATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTc                                            >  2:5647595/1‑149 (MQ=255)
                                                                                                        tCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCaat                                         >  2:3147846/1‑149 (MQ=255)
                                                                                                         cAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCaata                                        >  1:8581455/1‑149 (MQ=255)
                                                                                                         cAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCaata                                        >  1:5910068/1‑149 (MQ=255)
                                                                                                             aaaTATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAatga                                    <  2:7639515/148‑1 (MQ=255)
                                                                                                                tatTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATgatgg                                 >  1:9047047/1‑149 (MQ=255)
                                                                                                                 atTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATgatgga                                >  2:8610781/1‑149 (MQ=255)
                                                                                                                   ttATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATgatggatg                              >  2:1946627/1‑149 (MQ=255)
                                                                                                                     aTGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGaa                            >  2:7527160/1‑149 (MQ=255)
                                                                                                                      tGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAAt                           >  2:7023553/1‑149 (MQ=255)
                                                                                                                      tGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAAt                           <  1:7757313/149‑1 (MQ=255)
                                                                                                                       gAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAAtt                          >  1:1211922/1‑149 (MQ=255)
                                                                                                                         aCTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAg                        <  2:2731983/149‑1 (MQ=255)
                                                                                                                            tttATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAg                     >  1:2706740/1‑149 (MQ=255)
                                                                                                                             ttATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGt                    <  2:9475849/149‑1 (MQ=255)
                                                                                                                               aTCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATCCTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGa                  <  1:6075273/149‑1 (MQ=255)
                                                                                                                               aTCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGa                  <  1:8249940/149‑1 (MQ=255)
                                                                                                                                  aGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCa               <  2:4572221/149‑1 (MQ=255)
                                                                                                                                  aGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCa               >  1:1896530/1‑149 (MQ=255)
                                                                                                                                   gTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCaa              >  2:505333/1‑149 (MQ=255)
                                                                                                                                   gTGGCTTATGGACAGCCGCCTTGGTAACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCaa              >  2:5273833/1‑149 (MQ=255)
                                                                                                                                    tGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCAat             >  1:7969536/1‑149 (MQ=255)
                                                                                                                                     ggCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCAata            <  2:6836512/149‑1 (MQ=255)
                                                                                                                                     ggCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCAata            >  2:6603626/1‑149 (MQ=255)
                                                                                                                                             gACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCAATATCGCCAtt    <  2:6260175/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCAATTTGGATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGTCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCAATATCGCCATTCA  >  AM406671/294880‑295171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑