BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,081,552	T→A	V212D (GTT→GAT)	ldh →	L‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,081,552	0	T	A	99.8%	3493.8 / NA	900	V212D (GTT→GAT)	ldh	L‑lactate dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (1/1); major base A (415/481); minor base C (1/1); total (417/483)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 900 total aligned reads displayed.

TGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTCCTGTATCAGTATTC  >  AM406671/1081406‑1081697

 gACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGa                                                                                                                                                >  2:1791201/1‑149 (MQ=255)

  aCGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGaa                                                                                                                                               <  1:2030428/149‑1 (MQ=255)

    gTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAAtt                                                                                                                                             >  1:5534778/1‑149 (MQ=255)

     ttGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTg                                                                                                                                            >  1:3723260/1‑149 (MQ=255)

          gCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGa                                                                                                                                       >  2:1265282/1‑149 (MQ=255)

               ttCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAAAAATCGATGAATTGTTTGAGTCTg                                                                                                                                  >  1:8913816/1‑149 (MQ=255)

                   aTCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACg                                                                                                                              >  1:8457235/1‑149 (MQ=255)

                   aTCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCATAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACg                                                                                                                              <  2:5792188/149‑1 (MQ=255)

                     ccACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTg                                                                                                                            <  2:2984834/149‑1 (MQ=255)

                      cacacaTACAACATGGGAGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGa                                                                                                                           >  2:6691314/1‑149 (MQ=255)

                      cACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGt                                                                                                                                           <  2:8835362/133‑1 (MQ=255)

                       aCGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTAAGTGAt                                                                                                                          >  2:412515/1‑149 (MQ=255)

                        cGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATg                                                                                                                         <  2:2210205/149‑1 (MQ=255)

                            tACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGc                                                                                                                     >  2:2542792/1‑149 (MQ=255)

                             aCATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCt                                                                                                                    >  2:5251985/1‑149 (MQ=255)

                                          acacGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCg                                                                                                       <  1:4642377/149‑1 (MQ=255)

                                           cacGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGc                                                                                                      >  1:8723315/1‑149 (MQ=255)

                                            acGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCt                                                                                                     >  1:8804625/1‑149 (MQ=255)

                                            acGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCt                                                                                                     <  2:5470805/149‑1 (MQ=255)

                                              ggTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTaa                                                                                                   >  1:1444716/1‑149 (MQ=255)

                                               gTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTaaa                                                                                                  <  1:7041729/149‑1 (MQ=255)

                                                tGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTaaaa                                                                                                 >  2:604246/1‑149 (MQ=255)

                                                 gACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTaaaaa                                                                                                <  1:4476160/149‑1 (MQ=255)

                                                    tCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAgg                                                                                             >  1:9039460/1‑149 (MQ=255)

                                                     cAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGt                                                                                            >  1:6680798/1‑149 (MQ=255)

                                                        aaTTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAat                                                                                                            >  1:352546/1‑130 (MQ=255)

                                                        aaTTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAat                                                                                                            <  2:352546/130‑1 (MQ=255)

                                                          tttGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAAGTGGAAAAATGGTTCCAAGAAAATAACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATTCAGCTTACTCAATCATCGCTAAAAAAGGTGCAAc                                                                                       <  2:3165576/149‑1 (MQ=255)

                                                           ttGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACa                                                                                      <  2:2016655/149‑1 (MQ=255)

                                                           tggcggTTTGGTCACAGGCTAACGTTGCTGGTGTTAAAGTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGATAAAAAAGGTGCAACa                                                                                      <  2:750376/144‑1 (MQ=255)

                                                                gTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTa                                                                                 <  1:8136708/149‑1 (MQ=255)

                                                                 tttGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTAc                                                                                >  1:9165733/1‑149 (MQ=255)

                                                                 tttGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTAc                                                                                >  2:8650096/1‑149 (MQ=255)

                                                                      tCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGgtgt                                                                           >  1:3059930/1‑149 (MQ=255)

                                                                          acGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCt                                                                       <  1:4698934/149‑1 (MQ=255)

                                                                          acGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCt                                                                       <  1:7344512/149‑1 (MQ=255)

                                                                            gCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGt                                                                     <  1:7259582/149‑1 (MQ=255)

                                                                                aCGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGcc                                                                 <  1:4292897/149‑1 (MQ=255)

                                                                                 cGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGccc                                                                <  1:3994549/149‑1 (MQ=255)

                                                                                 cGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGccc                                                                >  2:7825329/1‑149 (MQ=255)

                                                                                 cGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGccc                                                                <  1:9585112/149‑1 (MQ=255)

                                                                                 cGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTAAGTGATGCAGCTTACTCAAAAAAAAAAAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGccc                                                                <  2:8007809/149‑1 (MQ=255)

                                                                                  gTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCt                                                               >  1:2493220/1‑149 (MQ=255)

                                                                                   ttGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCtt                                                              >  1:1266418/1‑149 (MQ=255)

                                                                                        ggTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCg                                                         >  1:6495440/1‑149 (MQ=255)

                                                                                        ggTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCg                                                         <  2:5064942/149‑1 (MQ=255)

                                                                                        ggTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCg                                                         >  1:6004285/1‑149 (MQ=255)

                                                                                         gtgtTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGt                                                        >  1:5926629/1‑149 (MQ=255)

                                                                                          tgtTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTa                                                       <  1:5137956/149‑1 (MQ=255)

                                                                                           gtTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATAATAGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTAt                                                      <  2:8938643/149‑1 (MQ=255)

                                                                                                 ttGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTaa                                                >  2:4596029/1‑149 (MQ=255)

                                                                                                 ttGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTaa                                                <  2:9286559/149‑1 (MQ=255)

                                                                                                  tGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTaaa                                               >  1:8350555/1‑149 (MQ=255)

                                                                                                   ggAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTGGTATTACTAAAg                                              >  2:3733886/1‑149 (MQ=255)

                                                                                                   ggAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAACGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAg                                              <  1:5253699/149‑1 (MQ=255)

                                                                                                    gAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGc                                             >  1:9302807/1‑149 (MQ=255)

                                                                                                     aaCAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCa                                            >  1:1646206/1‑149 (MQ=255)

                                                                                                      aCAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCaa                                           <  2:6255924/149‑1 (MQ=255)

                                                                                                       cAATGGTTCCAAGAAAATGACTACCTTAGCGAAGCAGAATTCGCTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAAt                                          <  1:6944831/149‑1 (MQ=255)

                                                                                                       cAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAAt                                          >  1:6163508/1‑149 (MQ=255)

                                                                                                       cAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAAt                                          >  2:3097620/1‑149 (MQ=255)

                                                                                                         aTGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTc                                        <  1:327891/149‑1 (MQ=255)

                                                                                                          tGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCt                                       >  1:2677533/1‑149 (MQ=255)

                                                                                                            gTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTtg                                     <  1:6830718/149‑1 (MQ=255)

                                                                                                            gTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTtg                                     >  2:7342626/1‑149 (MQ=255)

                                                                                                              tCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTtgat                                   <  2:4753361/149‑1 (MQ=255)

                                                                                                              tCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTtgat                                   >  2:6411636/1‑149 (MQ=255)

                                                                                                              tCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTtgat                                   <  1:8526462/149‑1 (MQ=255)

                                                                                                              tCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTtgat                                   >  2:8460382/1‑149 (MQ=255)

                                                                                                              tCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTtgat                                   <  1:1002269/149‑1 (MQ=255)

                                                                                                               ccAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTtgatg                                  <  1:1860305/149‑1 (MQ=255)

                                                                                                                cAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTtgatga                                 <  2:2462882/149‑1 (MQ=255)

                                                                                                                 aaGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGCAGCCCTTGCTCGTATTACTAAAGCAATTCTtgatgat                                >  2:5371439/1‑149 (MQ=255)

                                                                                                                  aGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTtgatgatg                               <  1:2367064/149‑1 (MQ=255)

                                                                                                                   gAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTtgatgatga                              <  1:9449484/149‑1 (MQ=255)

                                                                                                                     aaaTGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAAc                            <  2:1614155/149‑1 (MQ=255)

                                                                                                                     aaaTGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAAc                            <  1:7548613/149‑1 (MQ=255)

                                                                                                                     aaaTGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAAc                            >  2:9095495/1‑149 (MQ=255)

                                                                                                                        tGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATg                         >  1:630693/1‑149 (MQ=255)

                                                                                                                        tGACTACCTTAACGAAGCAGAAATCGATAAATTGTTTGAGTCTGTACGTGATGAAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTCCTAAAGCAGTTCTTGATGATGAAGATg                         >  2:5601972/1‑149 (MQ=255)

                                                                                                                          actactTTAACGAAGCAGAAACCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCa                       <  1:2916727/149‑1 (MQ=255)

                                                                                                                              ccTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTAc                   <  2:114380/149‑1 (MQ=255)

                                                                                                                              ccTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTAc                   <  1:2198202/149‑1 (MQ=255)

                                                                                                                                ttAACGAAGCAGAAATCTATGAATTGTTTAAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACtt                 <  2:1432811/149‑1 (MQ=255)

                                                                                                                                 tAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTc                >  2:6886290/1‑149 (MQ=255)

                                                                                                                                 tAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTc                >  1:7810996/1‑149 (MQ=255)

                                                                                                                                  aaCGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTcc               >  2:3865383/1‑149 (MQ=255)

                                                                                                                                   aCGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTCCt              >  2:2737136/1‑149 (MQ=255)

                                                                                                                                    cGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTCCTg             >  1:8015503/1‑149 (MQ=255)

                                                                                                                                       aGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTCCTGTAt          <  1:4879368/149‑1 (MQ=255)

                                                                                                                                       aGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTCCTGTAt          >  1:5438137/1‑149 (MQ=255)

                                                                                                                                           gAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTCCTGTATCAGt      >  2:8267764/1‑149 (MQ=255)

                                                                                                                                            aaaTCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTCCTGTATCAGTa     >  2:3633271/1‑149 (MQ=255)

                                                                                                                                            aaaTCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTCCTGTATCAGTa     <  2:4324212/149‑1 (MQ=255)

                                                                                                                                             aaTCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCGACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTCCTGTATCAGTAt    >  1:5766467/1‑149 (MQ=255)

                                                                                                                                             aaTCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTCCTGTATCAGTAt    <  2:7611913/149‑1 (MQ=255)

                                                                                                                                             aaTCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTCCTGTATCAGTAt    >  2:7147209/1‑149 (MQ=255)

                                                                                                                                             aaTCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTCCTGTATCAGTAt    >  2:6587373/1‑149 (MQ=255)

                                                                                                                                              aTCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTCCTGTATCAGTAtt   >  2:3341275/1‑149 (MQ=255)

                                                                                                                                               tCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTCCTGTATCAGTATTc  <  2:1908947/149‑1 (MQ=255)

TGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTCCTGTATCAGTATTC  >  AM406671/1081406‑1081697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`