Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 1,277,684 T→A N231I (AAT→ATT)  llmg_1306 ← conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM4066711,277,6840TA99.8% 3446.8 / NA 865N231I (AAT→ATT) llmg_1306conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (400/463);  minor base G (0/2);  total (400/465)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 865 total aligned reads displayed.

CTGAATTTTGTAAAATTTTACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCATTAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCTTTCAGGCC  >  AM406671/1277538‑1277829
                                                                                                                                                  |                                                                                                                                                 
 tGAATTTTGTAAAATTTTACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCCACAGCAAAACCTGCTTCAGTGGACACAGTTACTGTTCGCCCAGACTTCAAAAGCGGACCATTAGCTGCGGTCGGTGTCGTCGTCGTCTCAATaa                                                                                                                                                >  2:1686970/1‑149 (MQ=255)
    aTTTTGTAAAATTTTACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAg                                                                                                                                             <  1:3843256/149‑1 (MQ=255)
      tttGTAAAATTTTACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGaa                                                                                                                                           <  1:4859649/149‑1 (MQ=255)
        tGTAAAATTTTACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAAcc                                                                                                                                         <  2:4403562/149‑1 (MQ=255)
         gTAAAATTTTACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTAAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCg                                                                                                                                        <  2:5865655/149‑1 (MQ=255)
              aTTTTACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAgg                                                                                                                                   <  1:4361348/149‑1 (MQ=255)
                tttACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGt                                                                                                                                 >  2:8604886/1‑149 (MQ=255)
                  tACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGttt                                                                                                                               >  2:1463897/1‑149 (MQ=255)
                  tACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCCTTGTTGTCTCAATAAAAGAACCGAAAGGGttt                                                                                                                               >  2:4570066/1‑149 (MQ=255)
                    cTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTATCTGCGGTCGGTGTCGTTGTTGTCGCAATAAAAGAACCGAAAGGGTTTcc                                                                                                                             <  2:5247328/149‑1 (MQ=255)
                       gTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAGAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAg                                                                                                                          <  2:9016260/149‑1 (MQ=255)
                        tATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGa                                                                                                                         <  2:6487836/149‑1 (MQ=255)
                         aTCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGAt                                                                                                                        <  1:2191538/149‑1 (MQ=255)
                          tCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCGCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGCAAGGGTTTCCTAGAtg                                                                                                                       >  1:6024109/1‑149 (MQ=255)
                            tAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGAtgtg                                                                                                                     <  1:5027735/149‑1 (MQ=255)
                             aaCCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGc                                                                                                                    >  1:767914/1‑149 (MQ=255)
                             aaCCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGc                                                                                                                    <  2:574348/149‑1 (MQ=255)
                             aaCCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGc                                                                                                                    <  1:2006780/149‑1 (MQ=255)
                              aCCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCt                                                                                                                   <  1:1267257/149‑1 (MQ=255)
                               ccATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTGCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTc                                                                                                                  >  1:5639517/1‑149 (MQ=255)
                                cATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAACTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCTATAAAAGAACCGAAAGGGTTTCCTAGATATGCTcc                                                                                                                 >  2:6055090/1‑149 (MQ=255)
                                 aTGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCt                                                                                                                <  1:2380242/149‑1 (MQ=255)
                                  tGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCtt                                                                                                               <  2:3366186/149‑1 (MQ=255)
                                   gCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCttt                                                                                                              >  1:2611749/1‑149 (MQ=255)
                                   gCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCttt                                                                                                              <  2:9404115/149‑1 (MQ=255)
                                       tttGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTCGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTAttt                                                                                                          >  1:9367657/1‑149 (MQ=255)
                                                    tcCTACAGCAAAATCTGCTTCAGTGGAAAAAGTTACTGGTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTAttt                                                                                             <  1:7262016/149‑1 (MQ=255)
                                                     ccTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGAGCTCCTTTATTTTCAGGGTCTATTTc                                                                                            >  1:6167868/1‑149 (MQ=255)
                                                      cTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTcc                                                                                           >  2:9152223/1‑149 (MQ=255)
                                                      cTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTcc                                                                                           <  1:9223731/149‑1 (MQ=255)
                                                       tACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTccc                                                                                          >  2:2457147/1‑149 (MQ=255)
                                                         cAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAg                                                                                        >  2:3822182/1‑149 (MQ=255)
                                                          aGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGc                                                                                       >  1:4627717/1‑149 (MQ=255)
                                                             aaaaCCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTa                                                                                    <  1:3672872/149‑1 (MQ=255)
                                                              aaaCCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTaa                                                                                   <  1:8725146/149‑1 (MQ=255)
                                                              aaaCCTGCTTCAGTGGAAAAAGTTACTGGTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTaa                                                                                   <  1:8616684/149‑1 (MQ=255)
                                                               aaCCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAAt                                                                                  <  2:8368942/149‑1 (MQ=255)
                                                               aaCCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAAt                                                                                  >  2:6629750/1‑149 (MQ=255)
                                                                aCCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATc                                                                                 >  2:7992761/1‑149 (MQ=255)
                                                                 ccTGCTTCAGTGGAAAAAGTTACTGTACGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTANTTTCAGGGTCTATTTCCCAGCCTAATCt                                                                                <  1:5357695/149‑1 (MQ=255)
                                                                  cTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTAtt                                                                                              <  2:7085477/134‑1 (MQ=255)
                                                                  cTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTa                                                                               <  1:8916578/149‑1 (MQ=255)
                                                                  cTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTa                                                                               <  1:8293157/149‑1 (MQ=255)
                                                                  cTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTa                                                                               <  2:4055474/149‑1 (MQ=255)
                                                                   tGCGTCACTGGAACGAGTTGCTGTTCCCCCAGCATTTAAAAGCGGACTATTAGCTCCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAGCCGACACTGTGCCCCAGATGAGCGCCTTTATTTTCAGGGTCTCTTTCCCAGCCTAATCTAt                                                                              <  2:6315645/149‑1 (MQ=255)
                                                                    gCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGGCTATTTCCCAGCCTAATCTATg                                                                             <  2:3052524/149‑1 (MQ=255)
                                                                        cAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTa                                                                         <  1:3120561/149‑1 (MQ=255)
                                                                        cAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTa                                                                         <  1:1509938/149‑1 (MQ=255)
                                                                             gAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTatcatc                                                                    <  1:8441440/149‑1 (MQ=255)
                                                                                aaaGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATTTGCTCCTTTATTTTCAGCGTCTATTTCCCAGCCTAATCTATGATTatcatcatc                                                                 <  2:9534885/149‑1 (MQ=255)
                                                                                 aaGTTACTGTTCGCCCAGAATTTAAAAGCGGCCTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTAtcctcatca                                                                >  2:7405974/1‑149 (MQ=255)
                                                                                 aaGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTAtcatcatca                                                                <  1:3400838/149‑1 (MQ=255)
                                                                                   gTTACTGTTCGCCCAGAATTTAAACGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCAtt                                                              <  2:8220902/149‑1 (MQ=255)
                                                                                    ttACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTa                                                             >  2:3223108/1‑149 (MQ=255)
                                                                                     tACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTAc                                                            >  1:4481674/1‑149 (MQ=255)
                                                                                         gTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTttg                                                        >  2:7814722/1‑149 (MQ=255)
                                                                                            cGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTttgttg                                                     <  1:6338087/149‑1 (MQ=255)
                                                                                             gCCCAGAATTTAACAGCGGACTCTTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGCGTTTCCTAGATTTTCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTga                                                    <  2:1844785/149‑1 (MQ=255)
                                                                                             gCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTga                                                    <  1:4764550/149‑1 (MQ=255)
                                                                                               ccAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTgagg                                                  <  2:2055375/149‑1 (MQ=255)
                                                                                                 aGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTgaggag                                                >  1:2299209/1‑149 (MQ=255)
                                                                                                 aGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTgaggag                                                <  1:2789907/149‑1 (MQ=255)
                                                                                                   aaTTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTa                                              <  2:2731730/149‑1 (MQ=255)
                                                                                                    aTTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTAt                                             >  1:2996303/1‑149 (MQ=255)
                                                                                                    aTTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTAt                                             <  1:399952/149‑1 (MQ=255)
                                                                                                        aaaaGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCa                                         <  1:6566331/149‑1 (MQ=255)
                                                                                                         aaaGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCaa                                        <  2:4231804/149‑1 (MQ=255)
                                                                                                          aaGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAAt                                       <  2:2285650/149‑1 (MQ=255)
                                                                                                           aGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATa                                      <  1:4190885/149‑1 (MQ=255)
                                                                                                             cGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAgg                                    <  1:7992376/149‑1 (MQ=255)
                                                                                                             cGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAgg                                    >  2:7237384/1‑149 (MQ=255)
                                                                                                              ggACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGt                                   <  1:5186788/149‑1 (MQ=255)
                                                                                                              ggACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGt                                   >  2:2973002/1‑149 (MQ=255)
                                                                                                                aCTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTAt                                 >  1:990895/1‑149 (MQ=255)
                                                                                                                aCTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTAt                                 <  1:7113807/149‑1 (MQ=255)
                                                                                                                 cTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATc                                <  2:6912384/149‑1 (MQ=255)
                                                                                                                    ttAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCaaa                             >  1:5474003/1‑149 (MQ=255)
                                                                                                                     tAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAAt                            >  1:1792518/1‑149 (MQ=255)
                                                                                                                      aGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAAtt                           <  1:6899289/149‑1 (MQ=255)
                                                                                                                      aGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAAtt                           >  1:8155635/1‑149 (MQ=255)
                                                                                                                        cTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTta                         >  1:32512/1‑149 (MQ=255)
                                                                                                                         tGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTtat                        >  1:9552431/1‑149 (MQ=255)
                                                                                                                         tGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTtat                        >  1:7025516/1‑149 (MQ=255)
                                                                                                                              tcggtGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCa                   <  1:7846683/149‑1 (MQ=255)
                                                                                                                              tcggtGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCa                   <  2:5010932/149‑1 (MQ=255)
                                                                                                                                 gtgtCGTTGTTGTCTCAATAAAAAAACCGAAAGGGTTTCCTAGATGTGCCCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATa                >  2:484758/1‑149 (MQ=255)
                                                                                                                                  tgtCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAg               <  1:3471203/149‑1 (MQ=255)
                                                                                                                                      gttgttGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTcc           <  1:6687217/149‑1 (MQ=255)
                                                                                                                                      gttgttGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTcc           <  2:1167347/149‑1 (MQ=255)
                                                                                                                                       ttgttgTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTta                         <  2:5450771/134‑1 (MQ=255)
                                                                                                                                       ttgttgTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCt          >  1:1031002/1‑149 (MQ=255)
                                                                                                                                       ttgttgTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCt          >  2:9275298/1‑149 (MQ=255)
                                                                                                                                        tgttgtCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCtt         <  2:8837883/149‑1 (MQ=255)
                                                                                                                                        tgttgtCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCtt         >  2:3495400/1‑149 (MQ=255)
                                                                                                                                         gttgtCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCttt        >  2:1333346/1‑149 (MQ=255)
                                                                                                                                          ttgtCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCTTTc       <  2:3657268/149‑1 (MQ=255)
                                                                                                                                           tgtCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCAATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAAt                 >  1:5861972/1‑138 (MQ=255)
                                                                                                                                             tctcAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCTTTCAgg    >  1:7488286/1‑149 (MQ=255)
                                                                                                                                             tctcAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCTTTCAgg    <  1:2487044/149‑1 (MQ=255)
                                                                                                                                              ctcAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCTTTCAGGc   <  2:7665440/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CTGAATTTTGTAAAATTTTACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCATTAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCTTTCAGGCC  >  AM406671/1277538‑1277829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑