BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,492,365	T→A	L262L (CTA→CTT)	kinE ←	sensor kinase protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,492,365	0	T	A	99.6%	3363.0 / NA	890	L262L (CTA→CTT)	kinE	sensor kinase protein
Reads supporting (aligned to +/- strand): ref base T (2/1); major base A (447/434); minor base C (1/3); total (452/438)
Fisher's exact test for biased strand distribution p-value = 3.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 890 total aligned reads displayed.

GCAGGTTGAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGATAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAATTTTTTGATTTAATC  >  AM406671/1492219‑1492510

gCAGGTTGAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAg                                                                                                                                                 <  1:2277504/149‑1 (MQ=255)

gCAGGTTGAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAg                                                                                                                                                 >  2:8672217/1‑149 (MQ=255)

 cAGGTTGAATTTCACTGACCACTTTGTCAGTAAATTTAGTCAAGTTAAATTTTTCTTTATTTAATTCCAAATGATTTCTCCGAGAAATTGTTAGTAAATTTTTAATGAGATCTATCATTCTTAGGGACTATTTTTCAAATTAAGAAAgt                                                                                                                                                >  2:6447371/1‑148 (MQ=255)

    gTTGAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAg                                                                                                                                             >  1:5980319/1‑149 (MQ=255)

    gTTGAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAg                                                                                                                                             <  1:2135308/149‑1 (MQ=255)

     ttGAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTATTCATCAATATAAGAAAGCGAGt                                                                                                                                            >  2:1607351/1‑149 (MQ=255)

       gAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGtca                                                                                                                                          <  1:3087444/149‑1 (MQ=255)

       gAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGtca                                                                                                                                          <  1:9147048/149‑1 (MQ=255)

          tttCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGtcatca                                                                                                                                       <  1:8416635/149‑1 (MQ=255)

             cACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCaata                                                                                                                                    >  2:2730582/1‑149 (MQ=255)

               cTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCaataat                                                                                                                                  >  2:7459117/1‑149 (MQ=255)

                  aCCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTc                                                                                                                               >  2:233082/1‑149 (MQ=255)

                  aCCACTGTGCCAGTAAATTCAGTCAAGTTACATCTCTCTTTATCTACTTCGAAAGGGTTGCCCCGAGAAATTGACACTAGATTTGCAATGCGCACCTTCATTCTCAGGTACTCTCCATCAGTATAAGAACGCGAGTCCGCAATAATTTc                                                                                                                               <  2:4445582/149‑1 (MQ=255)

                     aCTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCggg                                                                                                                            <  1:7010810/149‑1 (MQ=255)

                      cTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGa                                                                                                                           >  1:4553344/1‑149 (MQ=255)

                      cTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTAAGGGCCTCTTCATCAATATAAGAAAGCGAGTCATCAATAttttggtga                                                                                                                           >  2:5668654/1‑144 (MQ=255)

                         tGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGc                                                                                                                        >  1:7679744/1‑149 (MQ=255)

                            cAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCa                                                                                                                     >  1:8714133/1‑149 (MQ=255)

                             aGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCAt                                                                                                                    >  1:5086281/1‑149 (MQ=255)

                              gTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATg                                                                                                                   <  1:8879721/149‑1 (MQ=255)

                               tacaTTCAGTCTCGTTAAATGTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGCCTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGAGGCTCATGc                                                                                                                  <  2:7926897/146‑1 (MQ=255)

                               tAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGc                                                                                                                  >  1:4094360/1‑149 (MQ=255)

                                   ttCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCAtc                                                                                                              <  1:748356/149‑1 (MQ=255)

                                   ttCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCAtc                                                                                                              >  2:2511753/1‑149 (MQ=255)

                                     cAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCAtctt                                                                                                            >  1:1005507/1‑149 (MQ=255)

                                       gTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCTTCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCCTGCCAtcttct                                                                                                          >  2:1825693/1‑149 (MQ=255)

                                        tCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATcttctt                                                                                                         <  1:1144409/149‑1 (MQ=255)

                                          aaGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCtttt                                                                                                       >  1:5555731/1‑149 (MQ=255)

                                           aGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCttttt                                                                                                      <  1:3928160/149‑1 (MQ=255)

                                           aGTTAAATTTTTCTTTATTTACTTCCAAATGATTCCCCCGAGAAATTATCAGTAAATTTTCAATTAGCTTTTTCATTCTCAGGGACTCTTCATTAATATAAGAAAACGAGTCATCAAAACTTTCCGCCTGGTCATGTCATCTTCttttt                                                                                                      >  2:1221439/1‑149 (MQ=255)

                                             ttAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTtaa                                                                                                    >  1:8592424/1‑149 (MQ=255)

                                                   ttttCTTTATTTACTTCCAAATGATTTCCCCGAGCAATTGTCAGTAAATTTTCAATGACCTCTTTCATTCCCAGGGGCTCTTCATCCATACAATAAAGCCAGTCATCAATCCTTTCGGGATGCTCATCGCGTCTCCTTTGTACTAACGt                                                                                              >  2:8214115/1‑149 (MQ=255)

                                                   tggtgcttagttcCTTCCACATGCTTTCCCCGAGAAATGGTCAGCAAATTTTCACTGAGCCCTTTCATTCTCAGGGACGCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCAGGCCATCTTCTTTTTAATAAAGt                                                                                              <  2:6826393/143‑1 (MQ=255)

                                                    tttCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTg                                                                                             >  1:5437018/1‑149 (MQ=255)

                                                      tCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTTAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATATTCTTTTTAATAAAGTGaa                                                                                           >  2:5089094/1‑148 (MQ=255)

                                                        tttatttaCTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACAt                                                                                         >  1:3775843/1‑149 (MQ=255)

                                                        tttatttaCTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACAt                                                                                         <  2:9044531/149‑1 (MQ=255)

                                                         ttatttaCTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATg                                                                                        >  1:1728030/1‑149 (MQ=255)

                                                          tatttaCTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGt                                                                                       <  1:7931827/149‑1 (MQ=255)

                                                           atttaCTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTc                                                                                      >  1:9556486/1‑149 (MQ=255)

                                                           atttaCTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAACCCTTTCCTTCTCAGGGACTCGTCATCTATATAAGAAAGCTAGTCATCAATAATTCCTGTATGCTAATGTCATCTTCTTTTTAACAAAGTTACATTTc                                                                                      >  2:101412/1‑149 (MQ=255)

                                                            tttaCTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCCTCTTCTTTTTAATAACGTGACATGTcc                                                                                     >  2:3135335/1‑149 (MQ=255)

                                                            tttaCTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTcc                                                                                     <  2:1399599/149‑1 (MQ=255)

                                                             ttaCTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATTAATATAAGAAACTGATTTATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTcta                                                                                    >  2:3329954/1‑147 (MQ=255)

                                                             ttaCTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCa                                                                                    <  2:6569590/149‑1 (MQ=255)

                                                               aCTTCCACATGATTTCCCCGAGAACTTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCCTCAATCTAAGAACGTGTGTCCTCTATAATTTCGGGATGTTCATGCCATCTTCTCTTGATTAACGTGACATGTCCACg                                                                                  >  2:7696541/1‑149 (MQ=255)

                                                                 ttCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAAtt                                                                                                                                 <  1:4278804/100‑1 (MQ=255)

                                                                 ttCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAAtt                                                                                                                                 >  2:5506836/1‑100 (MQ=255)

                                                                 ttCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGaa                                                                                >  1:2790809/1‑149 (MQ=255)

                                                                   ccAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCAATCTTCTTTTTAATAAAGTGACATGTCCACGAAtt                                                                              >  2:34518/1‑149 (MQ=255)

                                                                     aaaTGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTACATCAATATAAGAAAGCGAGTCATCAATATTTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTgc                                                                            >  2:5964806/1‑149 (MQ=255)

                                                                      aaTGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTgca                                                                           <  2:3790812/149‑1 (MQ=255)

                                                                       aTGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTgcag                                                                          >  2:1700513/1‑149 (MQ=255)

                                                                         gATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTgcagca                                                                        <  1:7814520/149‑1 (MQ=255)

                                                                          aTTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCaa                                                                       >  1:9269558/1‑149 (MQ=255)

                                                                           tttCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAAc                                                                      >  1:248871/1‑149 (MQ=255)

                                                                             tCCCCGAGAAATTTTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAATAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACgg                                                                    <  2:5849760/149‑1 (MQ=255)

                                                                             tCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACgg                                                                    <  1:1812481/149‑1 (MQ=255)

                                                                                 cGAGAAATTTTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTGTTCATCAATATAATAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGt                                                                <  2:194356/149‑1 (MQ=255)

                                                                                       aTTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAg                                                          >  1:3283765/1‑149 (MQ=255)

                                                                                         tGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGtt                                                        >  1:1289055/1‑149 (MQ=255)

                                                                                         tGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCACCAACGGGCGTCCTCAGtt                                                        >  2:4027606/1‑149 (MQ=255)

                                                                                            cAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCAt                                                     <  1:5677439/149‑1 (MQ=255)

                                                                                              gTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCAtgt                                                   >  1:1466349/1‑149 (MQ=255)

                                                                                               tAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCAtgtg                                                  <  1:6299212/149‑1 (MQ=255)

                                                                                                aaaTTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGa                                                 >  2:4244487/1‑149 (MQ=255)

                                                                                                   ttttCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGc                                              <  2:6664471/149‑1 (MQ=255)

                                                                                                        aaTGAGCCCTTTCATTCTCAGGGACTCTCCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATTTCCACGAATTGCAGCAACGGCCGTCCTCAGTTCATGTGAAGCATCCg                                         >  2:9400481/1‑149 (MQ=255)

                                                                                                         aTGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGa                                        <  1:319148/149‑1 (MQ=255)

                                                                                                          tGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGaa                                       <  1:4433842/149‑1 (MQ=255)

                                                                                                            aGCCCTTTCATTCTCAGGGGCTCTTCATCAATATAAGAAAGCGAGTCATCAATAATGTCGGGATGCTCATGCCATCTTCTGTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGaaac                                     <  1:7318985/149‑1 (MQ=255)

                                                                                                            aGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCAtct                                                                                                             <  2:629211/77‑1 (MQ=255)

                                                                                                            aGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGaaac                                     >  2:8559124/1‑149 (MQ=255)

                                                                                                              cccTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGaaacaa                                   >  1:3596773/1‑149 (MQ=255)

                                                                                                               ccTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGaaacaaa                                  >  1:1562948/1‑149 (MQ=255)

                                                                                                                cTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGaaacaaac                                 >  1:2029833/1‑149 (MQ=255)

                                                                                                                 tttCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAAct                                >  1:6557920/1‑149 (MQ=255)

                                                                                                                  ttCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGCAGCATCCGAAACACActg                               >  2:722863/1‑149 (MQ=255)

                                                                                                                    cATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAActgct                             >  1:5823360/1‑149 (MQ=255)

                                                                                                                       tctcAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTgctgct                          >  2:8871778/1‑149 (MQ=255)

                                                                                                                       tctcAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTgctgct                          >  1:6677626/1‑149 (MQ=255)

                                                                                                                        ctcAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTc                         <  1:8163019/149‑1 (MQ=255)

                                                                                                                        ctcAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTc                         <  1:897999/149‑1 (MQ=255)

                                                                                                                         tcAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCa                        <  1:4875691/149‑1 (MQ=255)

                                                                                                                         tcAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCa                        >  1:342332/1‑149 (MQ=255)

                                                                                                                           aGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCAtt                      >  1:295425/1‑149 (MQ=255)

                                                                                                                            gggACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATtc                     >  2:4886850/1‑149 (MQ=255)

                                                                                                                             ggACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCTTCCGAAACAAACTGCTGCTCATtct                    <  2:5263082/149‑1 (MQ=255)

                                                                                                                             ggACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATtct                    <  2:3660172/149‑1 (MQ=255)

                                                                                                                              gACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATtctc                   <  2:4745839/149‑1 (MQ=255)

                                                                                                                               aCTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCa                  <  1:4662249/149‑1 (MQ=255)

                                                                                                                                   ttCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAAttt              <  1:6159675/149‑1 (MQ=255)

                                                                                                                                     catcaATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAAttttt            >  2:2900359/1‑149 (MQ=255)

                                                                                                                                      atcaATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAAtttttt           >  1:6840825/1‑149 (MQ=255)

                                                                                                                                      atcaATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAAtttttt           >  1:6400495/1‑149 (MQ=255)

                                                                                                                                       tcaATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAATTTTTTg          <  2:2124341/149‑1 (MQ=255)

                                                                                                                                        caATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAATTTTTTGa         <  2:8417590/149‑1 (MQ=255)

                                                                                                                                        caATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAATTTTTTGa         >  1:5612382/1‑149 (MQ=255)

                                                                                                                                          atatAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAATTTTTTGAtt       <  2:6173051/149‑1 (MQ=255)

                                                                                                                                          atatAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAATTTTTTGAtt       >  1:5186904/1‑149 (MQ=255)

GCAGGTTGAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGATAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAATTTTTTGATTTAATC  >  AM406671/1492219‑1492510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`