Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 447,854 G→A A98T (GCT→ACT)  llmg_0454 → putative beta‑glucosides‑specific IIBC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM406671447,8540GA99.8% 4576.3 / NA 1187A98T (GCT→ACT) llmg_0454putative beta‑glucosides‑specific IIBC component
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base A (552/629);  total (552/635)
Fisher's exact test for biased strand distribution p-value = 2.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1196 total aligned reads displayed.

TACCTGGTGTCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTGCTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGATTATTAAGTATCTT  >  AM406671/447708‑447999
                                                                                                                                                  |                                                                                                                                                 
 aCCTGGTGTCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTtactg                                                                                                                                                >  2:8826771/1‑149 (MQ=255)
    tGGTGTCGCTGGAGCTGTTTATAACTCACACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGCCGCAAGCACTTACTGCaa                                                                                                                                             >  2:5601727/1‑149 (MQ=255)
    tGGTGTCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAATTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCaa                                                                                                                                             >  1:4023785/1‑149 (MQ=255)
       tgtCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAg                                                                                                                                          <  1:8122227/149‑1 (MQ=255)
             tGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCgg                                                                                                                                    >  2:5808755/1‑149 (MQ=255)
              ggAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGCTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGc                                                                                                                                   <  1:466018/149‑1 (MQ=255)
                aGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTa                                                                                                                                 <  1:7078424/149‑1 (MQ=255)
                 gCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAg                                                                                                                                <  2:2298828/149‑1 (MQ=255)
                 gCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAg                                                                                                                                <  1:2203688/149‑1 (MQ=255)
                  cTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGt                                                                                                                               >  2:4185643/1‑149 (MQ=255)
                    gTTTATAACTCAACCTTGAATCCATATCAAGTCGTAATTGGACAAGCTGTAGAAGATGTTTATCACGAGGTTGTTGAACAGCTTGGAGATTCCGCTGTTGATGAAGATGCACCGGCGCAAGCACTCACTGCATCAGCACCGGCTAGTgg                                                                                                                             >  2:1493596/1‑149 (MQ=255)
                     tttATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGt                                                                                                                            >  2:7016702/1‑149 (MQ=255)
                     tttATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGt                                                                                                                            >  1:2830048/1‑149 (MQ=255)
                      ttATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTa                                                                                                                           <  1:8246913/149‑1 (MQ=255)
                         taACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTaaaa                                                                                                                        <  1:3488598/149‑1 (MQ=255)
                         taACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTaaaa                                                                                                                        <  2:8366570/149‑1 (MQ=255)
                         taACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTaaaa                                                                                                                        <  1:4396170/149‑1 (MQ=255)
                             tCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACa                                                                                                                    >  1:1797927/1‑149 (MQ=255)
                               aaaCTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACaaa                                                                                                                  <  2:6008052/149‑1 (MQ=255)
                               aaaCTTGAATCAATATCAAGTAGTTATTCGACAAGCTGTCGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACaaa                                                                                                                  <  1:6928451/149‑1 (MQ=255)
                               aaaCTTGAATCAATATCAAGTAGTGATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAGCAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACaaa                                                                                                                  <  2:3576513/149‑1 (MQ=255)
                                aaCTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACaaaa                                                                                                                 <  2:8240141/149‑1 (MQ=255)
                                 aCTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAAt                                                                                                                <  2:9339029/149‑1 (MQ=255)
                                 aCTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAAt                                                                                                                >  2:1777805/1‑149 (MQ=255)
                                  cTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATc                                                                                                               >  2:3923957/1‑149 (MQ=255)
                                     gAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCaa                                                                                                            >  2:6161710/1‑149 (MQ=255)
                                      aaTCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAAt                                                                                                           >  1:6300668/1‑149 (MQ=255)
                                       aTCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAAtt                                                                                                          <  1:3704632/149‑1 (MQ=255)
                                        tCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTg                                                                                                         >  2:6703650/1‑149 (MQ=255)
                                         cAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGt                                                                                                        >  2:5470022/1‑149 (MQ=255)
                                          aaTATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAGCAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACAAGATAGAAAAAAAAAACAAAATCCAATTGtt                                                                                                       <  2:1954995/149‑1 (MQ=255)
                                           atatCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTc                                                                                                      <  1:9444163/149‑1 (MQ=255)
                                           atatCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTc                                                                                                      <  1:8429162/149‑1 (MQ=255)
                                             atCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCAt                                                                                                    <  2:7574915/149‑1 (MQ=255)
                                              tCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATg                                                                                                   >  2:65846/1‑149 (MQ=255)
                                              agaaatagaTATTGAACAAGCGGTAGAAGATGTTTATGACGAGGTAGTTAAACAGCAGGGAGAATCAGATGATGATGAAGATGCAACGGCGCAAGCACTTACTACAACAGAACCGTCTAGTGGTAAAAAACAAAATCCAATTGTTCATg                                                                                                   <  2:687795/144‑1 (MQ=255)
                                               cAAGTAGTTATTGGAAAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGc                                                                                                  >  2:5313408/1‑149 (MQ=255)
                                                  gtagtTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCttt                                                                                               >  2:2161644/1‑149 (MQ=255)
                                                    agtTATTGGACAAGCTGTAGAAGACGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGTCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTcc                                                                                             <  2:4087278/149‑1 (MQ=255)
                                                      ttATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCaa                                                                                           >  2:8134769/1‑149 (MQ=255)
                                                            gACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGtt                                                                                     >  2:3201411/1‑149 (MQ=255)
                                                             aCAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTa                                                                                    >  1:51402/1‑149 (MQ=255)
                                                                  cTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTggg                                                                               <  1:7650610/149‑1 (MQ=255)
                                                                  cTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTggg                                                                               >  1:1022768/1‑149 (MQ=255)
                                                                   tGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGa                                                                              >  1:1597703/1‑149 (MQ=255)
                                                                     tAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCACCAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACa                                                                            <  1:7472114/149‑1 (MQ=255)
                                                                     tAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACa                                                                            >  2:160557/1‑149 (MQ=255)
                                                                       gaagaTGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAAt                                                                          >  2:3371220/1‑149 (MQ=255)
                                                                       gaagaTGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAAt                                                                          <  1:1406009/149‑1 (MQ=255)
                                                                        aagaTGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAAtt                                                                         >  1:9180278/1‑149 (MQ=255)
                                                                          gaTGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTAc                                                                       >  2:9030309/1‑149 (MQ=255)
                                                                             gTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAgg                                                                    >  1:2964489/1‑149 (MQ=255)
                                                                              tttATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGt                                                                   >  1:3873344/1‑149 (MQ=255)
                                                                              tttATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGt                                                                   <  2:8642818/149‑1 (MQ=255)
                                                                                tATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTc                                                                 >  1:7772930/1‑149 (MQ=255)
                                                                                tATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGAGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTc                                                                 <  1:2030961/149‑1 (MQ=255)
                                                                                   caCGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCAGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGTACAATTACAGGTTCgat                                                              <  2:4267919/148‑1 (MQ=255)
                                                                                    aCGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCgatg                                                             >  2:5169624/1‑149 (MQ=255)
                                                                                     cGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCgatga                                                            <  2:1346305/149‑1 (MQ=255)
                                                                                      gAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCgatgat                                                           <  2:7314345/149‑1 (MQ=255)
                                                                                      gAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGATCgatgat                                                           >  1:5558084/1‑149 (MQ=255)
                                                                                        ggTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTc                                                         >  2:300610/1‑149 (MQ=255)
                                                                                        ggTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTc                                                         <  1:7299282/149‑1 (MQ=255)
                                                                                        ggTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTCGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTc                                                         <  1:4954875/149‑1 (MQ=255)
                                                                                           tgttgAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCaa                                                      <  1:6513998/149‑1 (MQ=255)
                                                                                             ttgAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAatt                                                    >  1:6097858/1‑149 (MQ=255)
                                                                                               gAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAat                                                     >  2:1048237/1‑146 (MQ=255)
                                                                                                 aCAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTg                                                <  2:5000913/149‑1 (MQ=255)
                                                                                                 aCAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTg                                                <  2:2830048/149‑1 (MQ=255)
                                                                                                   aGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGt                                              <  2:3699829/149‑1 (MQ=255)
                                                                                                     cTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATTCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGttt                                            >  1:8852255/1‑149 (MQ=255)
                                                                                                      ttGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTa                                           <  1:9326778/149‑1 (MQ=255)
                                                                                                       tGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTAc                                          <  2:1249825/149‑1 (MQ=255)
                                                                                                       tGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTAc                                          >  2:4869683/1‑149 (MQ=255)
                                                                                                        ggAGATTTAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCGAAGTGGTTATTGGGACAATTACAGGTTCGATGGTTCGAATTACTTGTTTACt                                         >  2:2587499/1‑149 (MQ=255)
                                                                                                        ggAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACt                                         <  1:420201/149‑1 (MQ=255)
                                                                                                          agaTTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTg                                       <  2:2424783/149‑1 (MQ=255)
                                                                                                          agaTTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTg                                       <  1:8987627/149‑1 (MQ=255)
                                                                                                             ttCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCgg                                    >  1:2532492/1‑149 (MQ=255)
                                                                                                             ttCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCgg                                    <  1:6426276/149‑1 (MQ=255)
                                                                                                              tCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGc                                   <  1:1248508/149‑1 (MQ=255)
                                                                                                              tCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGc                                   >  2:4479032/1‑149 (MQ=255)
                                                                                                              tCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATGCCAATTATTGGTTTACTTGCGGc                                   >  2:6421390/1‑149 (MQ=255)
                                                                                                                    gttgATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCtggtgg                             >  1:574422/1‑149 (MQ=255)
                                                                                                                    gttgATGAAGATGCAACGGCGCAAGCACNTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCtggtgg                             >  1:5359306/1‑149 (MQ=255)
                                                                                                                       gatgaAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGgat                          <  1:58235/149‑1 (MQ=255)
                                                                                                                        atgaAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGgatg                         <  2:7771952/149‑1 (MQ=255)
                                                                                                                         tgaAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGgatga                        <  2:7199895/149‑1 (MQ=255)
                                                                                                                             gATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTaa                    <  1:3372136/149‑1 (MQ=255)
                                                                                                                               tGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATGGGTTTACTTGCGGCTGGTGGGATGATTAATg                  <  1:3201411/149‑1 (MQ=255)
                                                                                                                                gCAACGGAGCAAGCACTCACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAAGTGTTCATGCTTTCCAAGTGGTTAGTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATgg                 <  2:9525714/149‑1 (MQ=255)
                                                                                                                                 cAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAAGCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGa                <  2:794936/149‑1 (MQ=255)
                                                                                                                                  aaCGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGat               <  1:8791856/149‑1 (MQ=255)
                                                                                                                                   aCGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGatt              >  1:2741024/1‑149 (MQ=255)
                                                                                                                                      gcgcAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGattatt           <  1:6703879/149‑1 (MQ=255)
                                                                                                                                        gcAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGATTATTaa         <  1:8684099/149‑1 (MQ=255)
                                                                                                                                         cAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGATTATTAAg        >  1:7932352/1‑149 (MQ=255)
                                                                                                                                          aaGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGATTATTAAGt       >  2:4624197/1‑149 (MQ=255)
                                                                                                                                             cACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGATTATTAAGTATc    <  2:4739716/149‑1 (MQ=255)
                                                                                                                                             cACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGATTATTAAGTATc    <  1:237021/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TACCTGGTGTCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTGCTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGATTATTAAGTATCTT  >  AM406671/447708‑447999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑