Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 1,783,142 G→A A603V (GCA→GTA)  llmg_1803 ← putative secreted protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM4066711,783,1420GA99.8% 3248.8 / NA 848A603V (GCA→GTA) llmg_1803putative secreted protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (409/437);  minor base C (0/2);  total (409/439)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 848 total aligned reads displayed.

GGATTTTATTAGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTGCCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGCCATTAGTGATAGACGT  >  AM406671/1782996‑1783287
                                                                                                                                                  |                                                                                                                                                 
ggATTTTATTAGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTAcc                                                                                                                                                 <  1:1006592/149‑1 (MQ=255)
  aTTTTATTAGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGt                                                                                                                                               >  2:2440609/1‑149 (MQ=255)
   ttttATTAGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGtt                                                                                                                                              <  2:8945952/149‑1 (MQ=255)
   ttttATGAGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACGGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCTATAGTTGAAGGTTACCGtt                                                                                                                                              >  2:4686926/1‑149 (MQ=255)
    tttATTAGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGttt                                                                                                                                             <  2:3188880/149‑1 (MQ=255)
    tttATTAGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGttt                                                                                                                                             >  1:7582559/1‑149 (MQ=255)
    tttATTAGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGttt                                                                                                                                             <  1:5966173/149‑1 (MQ=255)
     ttattaGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTtg                                                                                                                                            >  1:7363248/1‑149 (MQ=255)
     ttattaGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTATCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTtg                                                                                                                                            >  1:5786164/1‑149 (MQ=255)
      tattaGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTtgt                                                                                                                                           >  2:9490367/1‑149 (MQ=255)
      tattaGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCACTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTtgt                                                                                                                                           >  1:6090778/1‑149 (MQ=255)
        ttaGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGtgc                                                                                                                                         >  2:2079962/1‑149 (MQ=255)
        ttaGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGtgc                                                                                                                                         <  2:4885797/149‑1 (MQ=255)
         taGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGtgct                                                                                                                                        >  1:6329170/1‑149 (MQ=255)
         taGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGtgct                                                                                                                                        >  1:3379410/1‑149 (MQ=255)
          aGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGtgctg                                                                                                                                       >  1:7167711/1‑149 (MQ=255)
             cccTGTCGCTGTGGATGCTCTATTCCCGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGCCGTAGCCATAGTTGAAGGTTACCGTTTGtgctgctg                                                                                                                                    <  1:5396175/147‑1 (MQ=255)
               cTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTgctgctgct                                                                                                                                  >  1:49395/1‑149 (MQ=255)
                tGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGctgctgctg                                                                                                                                 <  2:407963/149‑1 (MQ=255)
                 gTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGt                                                                                                                                <  1:4343710/149‑1 (MQ=255)
                     cTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGc                                                                                                                            >  2:457326/1‑149 (MQ=255)
                        gggATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTg                                                                                                                         <  2:6624220/148‑1 (MQ=255)
                         ggATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCATGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGa                                                                                                                        >  2:1067932/1‑149 (MQ=255)
                         ggATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGa                                                                                                                        >  2:7151192/1‑149 (MQ=255)
                          gATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGAc                                                                                                                       <  1:6412105/149‑1 (MQ=255)
                            tGCTGTATTCCAGCCAGTTGGTGGTGTGACATTGGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATGGCAGGCGTGTTGAGGGCGCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCg                                                                                                                     <  2:7690307/149‑1 (MQ=255)
                             gCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGt                                                                                                                    <  1:2159910/149‑1 (MQ=255)
                               tGTATTCCAGCCAGTTGGTGGTGTGATATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGtat                                                                                                                  <  2:2247491/149‑1 (MQ=255)
                                 tATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAg                                                                                                                >  2:136932/1‑149 (MQ=255)
                                  aTTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTCTAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGt                                                                                                               <  2:5349588/149‑1 (MQ=255)
                                  aTTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGt                                                                                                               >  1:8670984/1‑149 (MQ=255)
                                   ttCCAGCCAGTTGGTCGTGAGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGACTGCTCCTGAGACGTAGGCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGGCGCTATAgga                                                                                                              >  2:6993974/1‑147 (MQ=255)
                                    tCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGttt                                                                                                             >  2:6786513/1‑148 (MQ=255)
                                    tCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTa                                                                                                             >  1:3001517/1‑149 (MQ=255)
                                    tCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTa                                                                                                             >  1:2351144/1‑149 (MQ=255)
                                     ccagccagTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATATTTAt                                                                                                            <  2:8687479/149‑1 (MQ=255)
                                      cagccagTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTAtt                                                                                                           <  2:1794857/149‑1 (MQ=255)
                                       agccagTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTACAGCTATTg                                                                                                          >  2:2832326/1‑149 (MQ=255)
                                            gTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGc                                                                                                     >  2:7255995/1‑149 (MQ=255)
                                            gTTGGTGGTGGGAGATTTGCTCCTGGGGTCCGAATCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGc                                                                                                     <  2:1900843/149‑1 (MQ=255)
                                             ttGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCt                                                                                                    <  1:926725/149‑1 (MQ=255)
                                                  ggtgTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGt                                                                                               >  2:8285556/1‑149 (MQ=255)
                                                   gtgtGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGtt                                                                                              <  2:8421096/149‑1 (MQ=255)
                                                     gtgACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTgc                                                                                            >  2:3992972/1‑149 (MQ=255)
                                                      tgACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTgcg                                                                                           <  2:1126952/149‑1 (MQ=255)
                                                      tgACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTgcg                                                                                           <  1:9362825/149‑1 (MQ=255)
                                                        aCATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCt                                                                                         >  1:7830121/1‑149 (MQ=255)
                                                              gCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTgt                                                                                   >  1:262072/1‑149 (MQ=255)
                                                               cTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTgtt                                                                                  >  2:5999656/1‑149 (MQ=255)
                                                                tCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTgttg                                                                                 >  2:1258207/1‑149 (MQ=255)
                                                                  cTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTgttggg                                                                               <  1:1942163/149‑1 (MQ=255)
                                                                   tGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTgttgggt                                                                              <  1:5142179/149‑1 (MQ=255)
                                                                          ccACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGc                                                                       <  1:5613762/149‑1 (MQ=255)
                                                                            aCTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCtg                                                                     >  2:2974131/1‑149 (MQ=255)
                                                                              tCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCtgtg                                                                   <  1:3506487/149‑1 (MQ=255)
                                                                                 cTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGaaa                                                                >  1:8449793/1‑149 (MQ=255)
                                                                                  tGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAg                                                               >  1:8368493/1‑149 (MQ=255)
                                                                                       ggCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGcc                                                          >  1:3721542/1‑149 (MQ=255)
                                                                                        gCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCa                                                         >  2:5534584/1‑149 (MQ=255)
                                                                                          tagtagGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAgt                                                       >  2:9336001/1‑149 (MQ=255)
                                                                                           agtagGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAgtg                                                      <  1:9515477/149‑1 (MQ=255)
                                                                                           agtagGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAgtg                                                      <  2:7523015/149‑1 (MQ=255)
                                                                                           agtagGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAgtg                                                      <  1:4117819/149‑1 (MQ=255)
                                                                                             tagGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTc                                                    >  1:4617004/1‑149 (MQ=255)
                                                                                                gTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCt                                                 <  1:1106270/149‑1 (MQ=255)
                                                                                                gTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCt                                                 >  1:9079929/1‑149 (MQ=255)
                                                                                                gTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCt                                                 >  2:2743095/1‑149 (MQ=255)
                                                                                                 tataGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTg                                                >  1:448268/1‑149 (MQ=255)
                                                                                                  ataGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGc                                               <  1:8771720/149‑1 (MQ=255)
                                                                                                    aGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTCTTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAg                                             <  2:6402148/149‑1 (MQ=255)
                                                                                                    aGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAg                                             >  1:4249129/1‑149 (MQ=255)
                                                                                                      cAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGtt                                           >  1:1598285/1‑149 (MQ=255)
                                                                                                      cAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGtt                                           >  1:7698506/1‑149 (MQ=255)
                                                                                                       aGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTg                                          <  1:6694176/149‑1 (MQ=255)
                                                                                                        ggCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGt                                         <  2:5135586/149‑1 (MQ=255)
                                                                                                          cGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCg                                       <  2:8070558/149‑1 (MQ=255)
                                                                                                          cGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCg                                       <  1:6254136/149‑1 (MQ=255)
                                                                                                              ttGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGTTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCAcc                                   >  1:2888473/1‑149 (MQ=255)
                                                                                                              ttGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCAcc                                   >  1:1706497/1‑149 (MQ=255)
                                                                                                                gAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAg                                 >  2:3536270/1‑149 (MQ=255)
                                                                                                                 aGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCa                                  >  1:403392/1‑147 (MQ=255)
                                                                                                                 aGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGt                                <  1:7069325/149‑1 (MQ=255)
                                                                                                                   tGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGtat                              >  1:3226391/1‑149 (MQ=255)
                                                                                                                    gCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGtata                             <  1:1365444/149‑1 (MQ=255)
                                                                                                                    gCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGtata                             <  2:2550848/149‑1 (MQ=255)
                                                                                                                    gCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGtata                             >  2:1585137/1‑149 (MQ=255)
                                                                                                                      tCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAg                           <  1:5733905/149‑1 (MQ=255)
                                                                                                                        cTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAATGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTa                         <  2:5814157/149‑1 (MQ=255)
                                                                                                                         tGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTAc                        <  2:3398753/149‑1 (MQ=255)
                                                                                                                         tGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTAc                        >  1:4781210/1‑149 (MQ=255)
                                                                                                                          gagaCGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACt                       <  2:8537417/149‑1 (MQ=255)
                                                                                                                            gaCGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACAAGTATAAGTACTCg                     <  2:914301/149‑1 (MQ=255)
                                                                                                                                tAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGcc                 >  2:8010992/1‑149 (MQ=255)
                                                                                                                                 aGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGCCa                >  1:8935470/1‑149 (MQ=255)
                                                                                                                                  gCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGCCAt               >  1:7980444/1‑149 (MQ=255)
                                                                                                                                      tAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAATTACTCGTGCCATTAGt           >  2:6204914/1‑149 (MQ=255)
                                                                                                                                        gTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGCCATTAGTGa         <  2:3771941/149‑1 (MQ=255)
                                                                                                                                          tGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGCCATTAGTGATa       <  2:4249129/149‑1 (MQ=255)
                                                                                                                                           gAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGCCATTAGTGATAg      <  1:8163475/149‑1 (MQ=255)
                                                                                                                                              ggTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGCCATTAGTGATAGACg   >  2:7861858/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGATTTTATTAGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTGCCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGCCATTAGTGATAGACGT  >  AM406671/1782996‑1783287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑