BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,817,902	C→A	E463D (GAG→GAT)	xylS ←	alpha‑xylosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,817,902	0	C	A	99.8%	3806.2 / NA	982	E463D (GAG→GAT)	xylS	alpha‑xylosidase
Reads supporting (aligned to +/- strand): ref base C (2/0); new base A (545/435); total (547/435)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 993 total aligned reads displayed.

GGATATTCATAGTAGAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGCTCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTCCACCAAGTATAGCCAA  >  AM406671/1817756‑1818047

ggATATTCATAGTAGAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGatc                                                                                                                                                 <  1:2707037/149‑1 (MQ=255)

  atatTCATAGTAGAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCtt                                                                                                                                               <  1:4280423/149‑1 (MQ=255)

    atTCATAGTAGAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGCTATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTa                                                                                                                                             >  2:2725961/1‑149 (MQ=255)

    atTCATAGTAGAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTa                                                                                                                                             >  2:5856620/1‑149 (MQ=255)

     ttCATAGTAGAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTAc                                                                                                                                            <  1:4069619/149‑1 (MQ=255)

      tCATAGTAGAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACt                                                                                                                                           >  1:8274260/1‑149 (MQ=255)

       cATAGTAGAGTGGTTCAACAAGTGCTCTACCTTCAGAAGTTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTa                                                                                                                                          <  1:6905817/149‑1 (MQ=255)

       cATAGTAGAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTa                                                                                                                                          >  1:6431058/1‑149 (MQ=255)

           gtagAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGa                                                                                                                                      >  2:2878355/1‑149 (MQ=255)

              gaatGGTTCAACAAGTGCTCTACCTTCAGAAGTTGTTATTAAATTGGCGCTATAAAGATATGTAATTAATTGATGGCGAAGTTATAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAAtt                                                                                                                                   <  2:6007798/146‑1 (MQ=255)

                    ttCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGAtt                                                                                                                             <  2:3281207/149‑1 (MQ=255)

                      caacaaGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGAtttt                                                                                                                           >  1:4884131/1‑149 (MQ=255)

                         caaGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAg                                                                                                                        <  1:7577062/149‑1 (MQ=255)

                           aGTGCTCTACCTTCAGAAGCTGCTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTCGAGGa                                                                                                                      >  1:1649359/1‑149 (MQ=255)

                               ctctACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGa                                                                                                                  <  1:2032593/149‑1 (MQ=255)

                                tctACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGaa                                                                                                                 <  2:9459369/149‑1 (MQ=255)

                                 ctACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCAGTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAg                                                                                                                <  2:2388664/149‑1 (MQ=255)

                                  tACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGt                                                                                                               >  1:5715504/1‑149 (MQ=255)

                                      ttCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCgat                                                                                                           <  1:1015076/149‑1 (MQ=255)

                                        cAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCgattg                                                                                                         >  2:5111712/1‑149 (MQ=255)

                                         aGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCgattga                                                                                                        <  2:9241749/149‑1 (MQ=255)

                                           aaGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCgattgatt                                                                                                      >  2:4615410/1‑149 (MQ=255)

                                           aaGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCgattgatt                                                                                                      >  1:1864930/1‑149 (MQ=255)

                                           aaGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCgattgatt                                                                                                      >  1:879023/1‑149 (MQ=255)

                                           aaGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCgattgatt                                                                                                      >  2:3516367/1‑149 (MQ=255)

                                            aGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGattgattg                                                                                                     >  1:4525343/1‑149 (MQ=255)

                                            aGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGattgattg                                                                                                     <  1:6802225/149‑1 (MQ=255)

                                             gCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTgg                                                                                                    >  2:6792767/1‑149 (MQ=255)

                                             gCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTgg                                                                                                    <  1:5910381/149‑1 (MQ=255)

                                                gTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGa                                                                                                 <  1:9138265/149‑1 (MQ=255)

                                                gTTAATAAACTGGCGATATTAATATAGGCAATTCATTGACCACGAAGTCGTAAAAATTCAAGCCTTGATTGTTCAGTCACCGCATCAAAACGCCAGGGACCTTTACTAGTGAATTCTCATTTTGAGGAATGAAGTCGAGTGATTAGTGa                                                                                                 <  2:1272327/149‑1 (MQ=255)

                                                  tattaAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGaaa                                                                                               >  1:2449745/1‑149 (MQ=255)

                                                   attaAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGaaaa                                                                                              <  2:1454431/149‑1 (MQ=255)

                                                       aaTTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATAcc                                                                                          >  2:4251347/1‑149 (MQ=255)

                                                        aTTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATAccc                                                                                         >  1:8566562/1‑149 (MQ=255)

                                                        aTTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATAccc                                                                                         <  1:9352574/149‑1 (MQ=255)

                                                         ttGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATAcccc                                                                                        >  2:8414601/1‑149 (MQ=255)

                                                         ttGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATAcccc                                                                                        >  1:7033110/1‑149 (MQ=255)

                                                         ttGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATAcccc                                                                                        >  2:3846876/1‑149 (MQ=255)

                                                           ggCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCaa                                                                                      >  2:2114539/1‑149 (MQ=255)

                                                            gcgcTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCaaa                                                                                     >  1:2904547/1‑149 (MQ=255)

                                                             cgcTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAAt                                                                                    <  1:7398620/149‑1 (MQ=255)

                                                             cgcTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAAt                                                                                    >  2:2585910/1‑149 (MQ=255)

                                                             cgcTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAAt                                                                                    >  1:9548201/1‑149 (MQ=255)

                                                                tataAAGATATGGAATTACTTGATGACGACGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGc                                                                                 <  2:5626369/149‑1 (MQ=255)

                                                                 ataAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATGTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCa                                                                                >  1:7136407/1‑149 (MQ=255)

                                                                   aaaGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAac                                                                              <  2:8087420/149‑1 (MQ=255)

                                                                     aGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAacca                                                                            >  2:3690370/1‑149 (MQ=255)

                                                                      gATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAaccac                                                                           >  1:6573791/1‑149 (MQ=255)

                                                                      gATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAaccac                                                                           >  1:8131356/1‑149 (MQ=255)

                                                                        tatGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCt                                                                         <  1:6313338/149‑1 (MQ=255)

                                                                         atGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCtt                                                                        >  2:8595235/1‑149 (MQ=255)

                                                                         atGGAATGAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGCTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATGTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCtt                                                                        <  1:3210725/149‑1 (MQ=255)

                                                                          tGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTa                                                                       >  2:1804284/1‑149 (MQ=255)

                                                                            gAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTaaa                                                                     >  2:2988084/1‑149 (MQ=255)

                                                                               ttaattGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTg                                                                  <  2:7130349/149‑1 (MQ=255)

                                                                               ttaattGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTg                                                                  >  1:559505/1‑149 (MQ=255)

                                                                                  attGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGc                                                               >  2:1583752/1‑149 (MQ=255)

                                                                                  attGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGc                                                               >  1:4822109/1‑149 (MQ=255)

                                                                                   ttGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCATCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCt                                                              <  1:9025878/149‑1 (MQ=255)

                                                                                   ttGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCt                                                              <  1:7280814/149‑1 (MQ=255)

                                                                                   ttGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCt                                                              <  1:1124406/149‑1 (MQ=255)

                                                                                   ttGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCt                                                              >  1:4162675/1‑149 (MQ=255)

                                                                                       tgaCGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGc                                                          >  1:7843535/1‑149 (MQ=255)

                                                                                       tgaCGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGc                                                          <  1:9252452/149‑1 (MQ=255)

                                                                                       tgaCGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGc                                                          >  1:6110841/1‑149 (MQ=255)

                                                                                          cGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATc                                                       <  1:5464783/149‑1 (MQ=255)

                                                                                           gAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCt                                                      <  1:2338789/149‑1 (MQ=255)

                                                                                              gTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCTCCTTAAACTGAGCTCCGCATCTTTg                                                   <  1:3946412/149‑1 (MQ=255)

                                                                                               ttGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGt                                                  <  2:8863536/149‑1 (MQ=255)

                                                                                                tGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTa                                                 >  1:3801696/1‑149 (MQ=255)

                                                                                                  tAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCtt                                                     <  1:7980638/143‑1 (MQ=255)

                                                                                                   aaaaaGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAAcc                                              <  2:1121097/149‑1 (MQ=255)

                                                                                                   aaaaaGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAAcc                                              <  2:6328931/149‑1 (MQ=255)

                                                                                                       aGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGc                                          <  1:3581060/149‑1 (MQ=255)

                                                                                                       aGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGc                                          >  2:4899595/1‑149 (MQ=255)

                                                                                                       aGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGGCGATTGATTGGTGAAAGTACCCCAAATTGCACCCACCTTAACCTGAGCTCCGCATCTTTGTAACCTTGc                                          <  2:5400650/149‑1 (MQ=255)

                                                                                                        gTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCa                                         <  1:8851943/149‑1 (MQ=255)

                                                                                                         tCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCAt                                        <  2:6115350/149‑1 (MQ=255)

                                                                                                          cAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCAtg                                       >  1:1400600/1‑149 (MQ=255)

                                                                                                             tCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGc                                    >  2:2019958/1‑149 (MQ=255)

                                                                                                              cATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATGTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGcc                                   >  2:7537408/1‑149 (MQ=255)

                                                                                                                ttgattgTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGcccc                                 >  2:792194/1‑149 (MQ=255)

                                                                                                                ttgattgTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGcccc                                 <  1:5098286/149‑1 (MQ=255)

                                                                                                                    ttgTTCAATCACCGCCTCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCaa                             <  2:318669/149‑1 (MQ=255)

                                                                                                                    ttgTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCaa                             >  2:7708630/1‑149 (MQ=255)

                                                                                                                    ttgTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGAGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCaa                             >  1:3361724/1‑149 (MQ=255)

                                                                                                                     tgTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAat                            >  2:4059284/1‑149 (MQ=255)

                                                                                                                      gTTCAATCACCGCATCAAAATGCCCGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAata                           >  2:6646208/1‑149 (MQ=255)

                                                                                                                        tCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATc                         <  1:8414601/149‑1 (MQ=255)

                                                                                                                            tCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATgg                     >  2:6199129/1‑149 (MQ=255)

                                                                                                                              aCCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCt                   >  1:3013323/1‑149 (MQ=255)

                                                                                                                                 gCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTcca                >  2:4379088/1‑149 (MQ=255)

                                                                                                                                    tCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTccacca             <  2:4812664/149‑1 (MQ=255)

                                                                                                                                    tCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAATCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTAAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCTCAATATCATGTCTccacca             <  2:6989969/149‑1 (MQ=255)

                                                                                                                                        aaTGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTCCACCAAGta         >  2:5494685/1‑149 (MQ=255)

                                                                                                                                          tGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTCCACCAAGtata       >  2:2223313/1‑149 (MQ=255)

                                                                                                                                           gCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTCCACCAAGTATAg      >  2:7298958/1‑149 (MQ=255)

                                                                                                                                           gCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATGGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCGCCACCAAGTATAg      >  1:5263793/1‑149 (MQ=255)

                                                                                                                                             cAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTCCACCAAGTATAGcc    >  2:9096730/1‑149 (MQ=255)

GGATATTCATAGTAGAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGCTCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTCCACCAAGTATAGCCAA  >  AM406671/1817756‑1818047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`