BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,854,998	T→A	V95V (GTA→GTT)	glgA ←	GlgA protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,854,998	0	T	A	99.9%	4453.6 / NA	1112	V95V (GTA→GTT)	glgA	GlgA protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base A (535/576); total (536/576)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1114 total aligned reads displayed.

ACATGCAAAATGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTTACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCAACAGAAG  >  AM406671/1854852‑1855143

aCATGCAAAATGTCAGGAATAAAATCTAATTTTTCTAAGAGATAACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCAAATAATAAAAAAAATAATATTTAAc                                                                                                                                                 <  2:1390979/149‑1 (MQ=255)

  aTGCAAAATGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCATCATAACCGTAAATCTACCCTCGACGAAAATAATCCTCACTATCTAAAAAATAATATTTAACAt                                                                                                                                               >  2:5723463/1‑149 (MQ=255)

  aTGCAAAATGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACAt                                                                                                                                               <  2:28229/149‑1 (MQ=255)

     cAAAATGTCAAGAATAAAATCTAATTATTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTAt                                                                                                                                            <  2:6424446/149‑1 (MQ=255)

       aaaTGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCt                                                                                                                                          <  2:5329004/149‑1 (MQ=255)

        aaTGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCtt                                                                                                                                         >  1:8028371/1‑149 (MQ=255)

        aaTGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCtt                                                                                                                                         <  2:7403914/149‑1 (MQ=255)

          tGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCtttt                                                                                                                                       >  1:7155144/1‑149 (MQ=255)

           atCAAGAATAAAATCTAATTATTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCAAAAAAAAAAAAAAATAATATTTAACATTATCTTTTa                                                                                                                                      <  2:5206891/148‑1 (MQ=255)

              aGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTa                                                                                                                                   <  2:510616/149‑1 (MQ=255)

              aGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTa                                                                                                                                   <  2:702955/149‑1 (MQ=255)

               ggAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTaa                                                                                                                                  >  1:1020220/1‑149 (MQ=255)

                  aTAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGt                                                                                                                               <  2:7218506/149‑1 (MQ=255)

                   tAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGtt                                                                                                                              <  1:532107/149‑1 (MQ=255)

                     aaaTCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGtttt                                                                                                                            >  2:5900891/1‑149 (MQ=255)

                     aaaTCTAAGGTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGtttt                                                                                                                            <  2:9034679/149‑1 (MQ=255)

                       aTCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTaa                                                                                                                          <  1:9330556/149‑1 (MQ=255)

                              ttttCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAAc                                                                                                                   >  2:1913984/1‑149 (MQ=255)

                              ttttCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAAc                                                                                                                   >  1:1764653/1‑149 (MQ=255)

                               tttCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACa                                                                                                                  <  1:5464846/149‑1 (MQ=255)

                                ttCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACat                                                                                                                 <  1:5193594/149‑1 (MQ=255)

                                ttCTAAGAGCTGACAAACGACTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACat                                                                                                                 <  1:4678772/149‑1 (MQ=255)

                                 tCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACata                                                                                                                >  2:4843242/1‑149 (MQ=255)

                                  cTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACatat                                                                                                               >  1:2632518/1‑149 (MQ=255)

                                      gagCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCAt                                                                                                           >  1:2290581/1‑149 (MQ=255)

                                        gCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATgc                                                                                                         <  1:2431033/149‑1 (MQ=255)

                                         cTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATgcc                                                                                                        >  1:2913878/1‑149 (MQ=255)

                                            aCAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCGACATATTCATgccgcc                                                                                                     >  1:6461173/1‑149 (MQ=255)

                                               aaCGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAAc                                                                                                  >  1:3644252/1‑149 (MQ=255)

                                                  ggCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTa                                                                                               <  1:7535498/149‑1 (MQ=255)

                                                   gCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCa                                                                                                            <  2:6583338/135‑1 (MQ=255)

                                                    cTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACa                                                                                             <  1:8140328/149‑1 (MQ=255)

                                                     tAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACAt                                                                                            >  2:6838272/1‑149 (MQ=255)

                                                       aaTCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATca                                                                                          <  1:4128244/149‑1 (MQ=255)

                                                           gAAAAAGGCAAAACGTTCACCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATcaacaa                                                                                      <  2:3478190/149‑1 (MQ=255)

                                                            aaaaaGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACaaa                                                                                     >  2:2951918/1‑149 (MQ=255)

                                                            aaaaaGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACaaa                                                                                     <  1:6805125/149‑1 (MQ=255)

                                                            aaaaaGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACaaa                                                                                     >  2:400899/1‑149 (MQ=255)

                                                            aaaaaGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACaaa                                                                                     >  1:3787989/1‑149 (MQ=255)

                                                             aaaaGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACaaaa                                                                                    >  1:3912083/1‑149 (MQ=255)

                                                              aaaGGCAAAACGTTCTCCATCATCACCATAACCTTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAAt                                                                                   <  2:6161113/149‑1 (MQ=255)

                                                                aGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACCTAAACAAAATCa                                                                                 >  2:4647963/1‑149 (MQ=255)

                                                                aGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCa                                                                                 >  1:9106388/1‑149 (MQ=255)

                                                                 ggCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTCAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCAt                                                                                >  2:1292021/1‑149 (MQ=255)

                                                                 ggCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCAt                                                                                <  1:6271946/149‑1 (MQ=255)

                                                                 ggCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCAt                                                                                <  2:330819/149‑1 (MQ=255)

                                                                  gCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATa                                                                               <  2:2428541/149‑1 (MQ=255)

                                                                   cAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATaa                                                                              <  2:7625898/149‑1 (MQ=255)

                                                                    aaaaCGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATaaa                                                                             <  1:9496662/149‑1 (MQ=255)

                                                                      aaCGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAAt                                                                           >  1:1159097/1‑149 (MQ=255)

                                                                       aCGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATcaa                                                                                         <  2:2017744/134‑1 (MQ=255)

                                                                       aCGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATcaa                                                                                         <  2:3760008/134‑1 (MQ=255)

                                                                       aCGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAAtt                                                                          <  1:5021301/149‑1 (MQ=255)

                                                                       aCGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAAtt                                                                          >  1:1406077/1‑149 (MQ=255)

                                                                        cGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTc                                                                         <  1:7756445/149‑1 (MQ=255)

                                                                          ttCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCAt                                                                       <  1:8659010/149‑1 (MQ=255)

                                                                           tctcCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATc                                                                      <  2:633310/149‑1 (MQ=255)

                                                                               catcatCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATcgacg                                                                  >  1:6664250/1‑149 (MQ=255)

                                                                                 tcatcaCCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGag                                                                <  2:8684788/149‑1 (MQ=255)

                                                                                    tcaCCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGagtag                                                             <  1:4800066/149‑1 (MQ=255)

                                                                                     caCCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGagtagt                                                            <  1:5966653/149‑1 (MQ=255)

                                                                                       ccATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGa                                                          <  2:3159958/149‑1 (MQ=255)

                                                                                        cATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGaa                                                         <  2:3630607/149‑1 (MQ=255)

                                                                                            aCCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGat                                                     <  2:8833626/149‑1 (MQ=255)

                                                                                               gTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATAtt                                                  <  2:4503254/149‑1 (MQ=255)

                                                                                               gTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATAtt                                                  <  1:4491231/149‑1 (MQ=255)

                                                                                                   aTCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATAttcttc                                              >  1:8326017/1‑149 (MQ=255)

                                                                                                     cTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTcttctt                                            <  2:1020220/149‑1 (MQ=255)

                                                                                                      tGCCCTCGGCCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCttt                                           <  1:5539649/149‑1 (MQ=255)

                                                                                                      tGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCttt                                           >  2:8284993/1‑149 (MQ=255)

                                                                                                        cccTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCtttca                                         <  1:4288278/149‑1 (MQ=255)

                                                                                                        cccTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCtttca                                         <  2:7709673/149‑1 (MQ=255)

                                                                                                         ccTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAATTTCATCGAAGTGTAGTTAACCTTATTCTTCtttcat                                        <  2:949322/149‑1 (MQ=255)

                                                                                                         ccTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCtttcat                                        <  1:8274826/149‑1 (MQ=255)

                                                                                                           tCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCtttcattt                                      >  2:4148131/1‑149 (MQ=255)

                                                                                                           tCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCtttcattt                                      <  2:8028371/149‑1 (MQ=255)

                                                                                                              aCCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTttcatttcat                                   >  1:84631/1‑149 (MQ=255)

                                                                                                               ccAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTtcatatcatt                                  >  1:7390474/1‑149 (MQ=255)

                                                                                                                cAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTcatttcattt                                 >  1:8793380/1‑149 (MQ=255)

                                                                                                                  aaaTAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCAttttt                               >  2:9274630/1‑149 (MQ=255)

                                                                                                                  aaaTAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCAttttt                               <  1:6968119/149‑1 (MQ=255)

                                                                                                                     taatGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCACCAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGaa                            >  1:2114694/1‑149 (MQ=255)

                                                                                                                     taatGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGaa                            <  2:6712735/149‑1 (MQ=255)

                                                                                                                     taatGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGaa                            <  2:2225882/149‑1 (MQ=255)

                                                                                                                     taatGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGaa                            <  2:3874649/149‑1 (MQ=255)

                                                                                                                      aatGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGaaa                           >  2:2697853/1‑149 (MQ=255)

                                                                                                                        tGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATa                         >  1:8975525/1‑149 (MQ=255)

                                                                                                                          cTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAg                       <  1:1669156/149‑1 (MQ=255)

                                                                                                                            cATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGt                     >  1:3311555/1‑149 (MQ=255)

                                                                                                                               tATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTaaa                  >  2:8463190/1‑149 (MQ=255)

                                                                                                                                aTCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTaaaa                 >  1:3053925/1‑149 (MQ=255)

                                                                                                                                    aaaaaaTAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATcac             >  1:4588405/1‑149 (MQ=255)

                                                                                                                                     aaaaaTAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATcaca            <  2:1550966/149‑1 (MQ=255)

                                                                                                                                     aaaaaTAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATcaca            >  2:431277/1‑149 (MQ=255)

                                                                                                                                        aataatATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCa         >  2:5469029/1‑149 (MQ=255)

                                                                                                                                        aataatATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCa         <  1:1913984/149‑1 (MQ=255)

                                                                                                                                          taataTTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCAAc       >  2:253175/1‑149 (MQ=255)

                                                                                                                                              atTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCAACagaa   >  1:5753041/1‑149 (MQ=255)

                                                                                                                                              atTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCAACagaa   <  1:6151320/149‑1 (MQ=255)

                                                                                                                                              atTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCAACagaa   >  2:16914/1‑149 (MQ=255)

ACATGCAAAATGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTTACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCAACAGAAG  >  AM406671/1854852‑1855143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`