Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 617,310 A→C S8S (TCA→TCC)  llmg_0628 → divalent cation transport‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM406671617,3100AC97.3% 4237.1 / ‑5.6 1111S8S (TCA→TCC) llmg_0628divalent cation transport‑related protein
Reads supporting (aligned to +/- strand):  ref base A (3/27);  new base C (582/496);  total (588/523)
Fisher's exact test for biased strand distribution p-value = 8.68e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1118 total aligned reads displayed.

CTTTTCGTTAAGGCTTTGAAATAAAATAATGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCAAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAATTTTAGATGACTATGAAAATG  >  AM406671/617164‑617455
                                                                                                                                                  |                                                                                                                                                 
 ttttCGTTAAGGCTTTGAAATAAAATAATGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAAt                                                                                                                                                <  1:1043602/149‑1 (MQ=255)
    tCGTTAAGGCTTTGAAATAAAATAATGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCcaaaaaa                                                                                                                                             <  2:8395939/149‑5 (MQ=255)
         aaGGCTTTGAAATAAAATAATGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGaaaaaaa                                                                                                                                        >  1:3530499/1‑149 (MQ=255)
          aGGCTTTGAAATAAAATAATGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGaaaaaaaa                                                                                                                                       >  1:1297708/1‑149 (MQ=255)
               ttGAAATAAAATAATGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGAt                                                                                                                                  >  1:1166856/1‑149 (MQ=255)
                tGAAATAAAATAATGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGAtc                                                                                                                                 <  1:398586/149‑1 (MQ=255)
                    ataaaataaTGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCaa                                                                                                                             >  1:7132728/1‑149 (MQ=255)
                     taaaataaTGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAAc                                                                                                                            <  1:776208/149‑1 (MQ=255)
                          taatGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTg                                                                                                                       <  2:489459/149‑1 (MQ=255)
                          taatGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTg                                                                                                                       >  2:6165373/1‑149 (MQ=255)
                          taatGAAAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATGCAATGAAAAAAAATTGATCTCAACTTCTg                                                                                                                       <  2:5956944/149‑1 (MQ=255)
                             tGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTgaga                                                                                                                    <  2:8064773/149‑1 (MQ=255)
                              gagaAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGAt                                                                                                                   <  1:1525946/149‑1 (MQ=255)
                              gagaAAAAAATAGGAAAATCTGCTACAANTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGAt                                                                                                                   >  1:5308827/1‑149 (MQ=255)
                               agaAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATAAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACAATACAATGAAAAAAAATTGATCTCAACTTCTGAGATg                                                                                                                  <  2:1595164/149‑1 (MQ=255)
                                gaAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATga                                                                                                                 <  1:6288849/149‑1 (MQ=255)
                                 aaaaaaaTAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATgaa                                                                                                                >  1:231035/1‑149 (MQ=255)
                                 aaaaaaaTAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATgaa                                                                                                                <  1:4469638/149‑1 (MQ=255)
                                    aaaaTAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATgaagaa                                                                                                             <  1:8682307/149‑1 (MQ=255)
                                         aGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCa                                                                                                        >  1:4807870/1‑149 (MQ=255)
                                          ggAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCAc                                                                                                       >  2:6818260/1‑149 (MQ=255)
                                            aaaaTCTGCTACAATTAGAAGGAGAAGAAGAGGATTCAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACtt                                                                                                     <  2:9483549/149‑1 (MQ=255)
                                             aaaacagCTACAATTAGAAGAAGAAGAAGAGGATTTAAATCCTTATTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATAATGAAATAAACAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTa                                                                                                    <  2:1186924/143‑1 (MQ=255)
                                              aaTCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTAt                                                                                                   <  2:7126026/149‑1 (MQ=255)
                                                 cTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGTCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTc                                                                                                <  1:3856664/149‑1 (MQ=255)
                                                  tGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTATGTAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTcc                                                                                               >  2:3803135/1‑149 (MQ=255)
                                                    cTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTc                                                                                             >  1:957073/1‑149 (MQ=255)
                                                     tACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACGATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCa                                                                                            <  2:3915878/149‑1 (MQ=255)
                                                      aCAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCaa                                                                                           >  1:2526854/1‑149 (MQ=255)
                                                       cAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAAt                                                                                          >  2:4453168/1‑149 (MQ=255)
                                                        aaTTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATc                                                                                         <  1:4131309/149‑1 (MQ=255)
                                                         aTTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATcc                                                                                        >  1:8843561/1‑149 (MQ=255)
                                                           tAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGAGCTCAACTTCTGAGATGAAGAATTTCACTTATGTGCTCAATCCaa                                                                                      >  2:9238579/1‑149 (MQ=255)
                                                            aGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAac                                                                                     <  2:511602/149‑1 (MQ=255)
                                                             gAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAaca                                                                                    >  1:8189988/1‑149 (MQ=255)
                                                             gAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCACAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAaca                                                                                    >  2:4713788/1‑149 (MQ=255)
                                                                  agaagaagaGGATTTAAAGGCTTTTTTATCAGGAAAAGAAGCGATAGATAGGCTGATATGAGAAAAAATTATAAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTga                                                                               <  2:6053847/149‑1 (MQ=255)
                                                                     agaagaGGATTTAAATCCTTTTTTATTAGGAACAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCATCGCGTgaaga                                                                            >  2:2585381/1‑149 (MQ=255)
                                                                        agagGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTg                                                                                                                                    >  2:6605168/1‑90 (MQ=255)
                                                                        agagGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAAt                                                                         >  1:1686664/1‑149 (MQ=255)
                                                                        agagGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAAt                                                                         >  1:7453732/1‑149 (MQ=255)
                                                                            gATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTggg                                                                     >  2:7704358/1‑149 (MQ=255)
                                                                              tttAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGaa                                                                   >  2:9078742/1‑149 (MQ=255)
                                                                              tttAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCATCAATCCAACACGTGAAGAAATTGGGaa                                                                   >  2:3128778/1‑149 (MQ=255)
                                                                                tAAATCCTTTTTTATTAGTAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAata                                                                 >  2:2947425/1‑149 (MQ=255)
                                                                                tAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAata                                                                 >  1:6992254/1‑149 (MQ=255)
                                                                                 aaaTCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAatat                                                                <  2:1900380/149‑1 (MQ=255)
                                                                                  aaTCCTTTTTTATTAGGTAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATAtc                                                               >  2:3450154/1‑149 (MQ=255)
                                                                                  aaTCCTTTTTTATTAGGAAAAGAAGGGATAGCTACGCTGATCTGATAAACAATTATGAACTATCGAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTACTCAATCCAACACGTGAAGAAATTGGGAATAtc                                                               >  2:4030978/1‑149 (MQ=255)
                                                                                  aaTCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCTTCAATCCAACACGTGAAGAAATTGGGAATAtc                                                               >  2:2297619/1‑149 (MQ=255)
                                                                                  aaTCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAatatt                                                               >  2:3299537/1‑148 (MQ=255)
                                                                                   aTCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATAAAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATAtct                                                              <  2:4196931/149‑1 (MQ=255)
                                                                                    tCCTTTTTTATTTGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATAtctc                                                             >  2:5807294/1‑149 (MQ=255)
                                                                                      cTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAg                                                           <  1:6418844/149‑1 (MQ=255)
                                                                                      cTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAg                                                           >  1:7609425/1‑149 (MQ=255)
                                                                                       ttttttATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGa                                                          >  2:1065036/1‑149 (MQ=255)
                                                                                        tttttATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCaa                                                                                           <  1:726779/115‑1 (MQ=255)
                                                                                        tttttATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGaa                                                         <  2:8702642/149‑1 (MQ=255)
                                                                                         ttttATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAac                                                        <  1:57719/149‑1 (MQ=255)
                                                                                            tattaGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACt                                                     <  1:3710746/149‑1 (MQ=255)
                                                                                             attaGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTa                                                    >  2:8241620/1‑149 (MQ=255)
                                                                                              ttaGGAAAAGAAGGAATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTAt                                                   <  2:36333/149‑1 (MQ=255)
                                                                                                    aaaGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGAtttt                                             <  2:8578016/149‑1 (MQ=255)
                                                                                                     aagaagGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTc                                            >  2:7295088/1‑149 (MQ=255)
                                                                                                      agaagGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTcc                                           <  1:9402556/149‑1 (MQ=255)
                                                                                                      agaagGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTcc                                           >  1:2698083/1‑149 (MQ=255)
                                                                                                        aagGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCtt                                         >  1:5176434/1‑149 (MQ=255)
                                                                                                            gatagataGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGa                                     >  1:4309146/1‑149 (MQ=255)
                                                                                                             atagatagGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGAc                                    <  1:2996774/149‑1 (MQ=255)
                                                                                                              tagatagGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGAct                                   >  1:8351240/1‑149 (MQ=255)
                                                                                                               agatagGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGActa                                  <  2:8861584/149‑1 (MQ=255)
                                                                                                               agatagGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGActa                                  >  1:5897221/1‑149 (MQ=255)
                                                                                                                  tagGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGActatct                               <  2:7563601/149‑1 (MQ=255)
                                                                                                                  tagGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGActatct                               >  1:6652014/1‑149 (MQ=255)
                                                                                                                   agGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGActatcta                              <  1:2017090/149‑1 (MQ=255)
                                                                                                                    ggCTGATATGATAAAAAATTATGAACTATCAAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGActatctat                             <  2:7002736/149‑1 (MQ=255)
                                                                                                                     gCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACtatctatc                            >  1:3376255/1‑149 (MQ=255)
                                                                                                                       tgatatgataAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTg                          >  2:7934751/1‑149 (MQ=255)
                                                                                                                        gatatgataAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTgg                         >  1:9010541/1‑149 (MQ=255)
                                                                                                                        gatatgataAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTgg                         >  2:4572306/1‑149 (MQ=255)
                                                                                                                          tatgataAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGaa                       <  1:6059456/149‑1 (MQ=255)
                                                                                                                            tgataAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAAtt                     >  1:8083239/1‑149 (MQ=255)
                                                                                                                             gataAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAAttt                    <  2:2145416/149‑1 (MQ=255)
                                                                                                                             gataAAAAATTATGAACTATACAAAGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAAttt                    <  2:1288713/149‑1 (MQ=255)
                                                                                                                              ataAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAAtttt                   >  1:2445307/1‑149 (MQ=255)
                                                                                                                                aaaaaaTTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAATTTTAg                 >  2:2013024/1‑149 (MQ=255)
                                                                                                                                  aaaaTTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAATTTTAGAt               <  1:2173530/149‑1 (MQ=255)
                                                                                                                                   aaaTTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATCATTTTCCTTTTGACTATCTATCTGGAATTTTAGATg              <  2:6424563/149‑1 (MQ=255)
                                                                                                                                   aaaTTATGAACTATCCAATGAAAAAAAATTGATCTCAACTACTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAATTTTAGATg              <  2:2389953/149‑1 (MQ=255)
                                                                                                                                     aTTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAATTTTAGATGAc            <  1:2872481/149‑1 (MQ=255)
                                                                                                                                       tATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATGCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAATTTTAGATGACTa          >  1:4946101/1‑149 (MQ=255)
                                                                                                                                       tATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAATTTTAGATGACTa          <  1:6844953/149‑1 (MQ=255)
                                                                                                                                        aTGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAATTTTAGATGACTAt         >  2:274172/1‑149 (MQ=255)
                                                                                                                                         tGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAATTTTAGATGACTATg        <  1:3191861/149‑1 (MQ=255)
                                                                                                                                          gAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAACTTTAGATGACTATGa       <  2:5318807/149‑1 (MQ=255)
                                                                                                                                           aaCTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAATTTTAGATGACTATGaa      >  1:9228492/1‑149 (MQ=255)
                                                                                                                                           aaCTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAATTTTAGATGACTATGaa      >  1:1430907/1‑149 (MQ=255)
                                                                                                                                            aCTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAATTTTAGATGACTATGaaa     <  2:5511150/149‑1 (MQ=255)
                                                                                                                                            aCTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAATTTTAGATGACTATGaaa     >  1:7934699/1‑149 (MQ=255)
                                                                                                                                             cTATCCAACGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAATTTTAGATGACTATGaaaa    <  1:5557499/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CTTTTCGTTAAGGCTTTGAAATAAAATAATGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCAAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAATTTTAGATGACTATGAAAATG  >  AM406671/617164‑617455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑