BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	2,266,724	T→C	I10T (ATT→ACT)	argR →	arginine repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	2,266,724	0	T	C	99.5%	5356.8 / NA	1300	I10T (ATT→ACT)	argR	arginine repressor
Reads supporting (aligned to +/- strand): ref base T (1/1); major base C (658/633); minor base A (2/4); total (661/639)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1318 total aligned reads displayed.

ACCATCGATTGCAGCAGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTATTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACGGGACAAAGGAGAG  >  AM406671/2266578‑2266869

    tCGATTGCAGCAGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGa                                                                                                                                             >  2:9263170/1‑149 (MQ=255)

     cGATTGCAGCAGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGaa                                                                                                                                            <  1:5126458/149‑1 (MQ=255)

        ttGCAGCAGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAAtt                                                                                                                                         >  1:4819524/1‑149 (MQ=255)

         tGCAGCAGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATtg                                                                                                                                        >  1:3836880/1‑149 (MQ=255)

           cagcagACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATtgtg                                                                                                                                      <  2:80926/149‑1 (MQ=255)

           aatcagACAGTGCTTCGCTTACTAGTTGTTACTCGTCCATTCAATAATTATACATAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGTAATTACTAAAGAAATtgtg                                                                                                                                      <  2:2378612/146‑1 (MQ=255)

              cagACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACa                                                                                                                                   >  1:4637939/1‑149 (MQ=255)

              cagACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACa                                                                                                                                   >  2:9096330/1‑149 (MQ=255)

                gACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGCGATAAAAGATTAGAAATTACTAAAGAAATTGTGACaaa                                                                                                                                 >  1:5893275/1‑149 (MQ=255)

                gACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACaaa                                                                                                                                 <  1:7088886/149‑1 (MQ=255)

                gACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACaaa                                                                                                                                 <  2:5833978/149‑1 (MQ=255)

                 aCAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAaat                                                                                                                                >  1:9185324/1‑149 (MQ=255)

                 aCAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAaat                                                                                                                                <  2:1330593/149‑1 (MQ=255)

                  cAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAaata                                                                                                                               <  2:566441/149‑1 (MQ=255)

                  cAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAaata                                                                                                                               <  1:2730329/149‑1 (MQ=255)

                     tGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAataata                                                                                                                            <  2:3714564/149‑1 (MQ=255)

                         tGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAat                                                                                                                        >  1:2185842/1‑149 (MQ=255)

                           gCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAatat                                                                                                                      <  1:8512207/149‑1 (MQ=255)

                           gCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACNAAAGAAATTGTGACAAATAATAAAatat                                                                                                                      <  1:5333219/149‑1 (MQ=255)

                              tACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGGGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGa                                                                                                                   >  1:1818965/1‑149 (MQ=255)

                              tACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGa                                                                                                                   >  1:2835634/1‑149 (MQ=255)

                              tACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGa                                                                                                                   <  2:6078414/149‑1 (MQ=255)

                                  aGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCa                                                                                                               >  2:3331069/1‑149 (MQ=255)

                                  aGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCa                                                                                                               >  1:3231020/1‑149 (MQ=255)

                                  aGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCa                                                                                                               <  2:5976246/149‑1 (MQ=255)

                                    ttgttTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGGCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCaag                                                                                                             <  2:1627035/149‑1 (MQ=255)

                                           tCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCt                                                                                                      <  1:1176202/149‑1 (MQ=255)

                                            cGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCtt                                                                                                     >  2:1454258/1‑149 (MQ=255)

                                             gTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTc                                                                                                    <  1:2544179/149‑1 (MQ=255)

                                                cATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAAt                                                                                                 >  2:2977757/1‑149 (MQ=255)

                                                  tgcACTAATTATCAAAAAAAAACACTAATTTTGTCAATATATCGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGACCTTCAAtct                                                                                               <  2:2198445/147‑1 (MQ=255)

                                                   tCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAAtctc                                                                                              >  1:1019794/1‑149 (MQ=255)

                                                       tAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCtatt                                                                                          <  2:3981249/149‑1 (MQ=255)

                                                        aaTTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCtatta                                                                                         >  2:8809884/1‑149 (MQ=255)

                                                        aaTTATACAGAAAAAACACTAATTTTGTCAATATATAGTACATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAACTTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCtatta                                                                                         <  2:7733078/149‑1 (MQ=255)

                                                         aTTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCtattat                                                                                        >  1:2343455/1‑149 (MQ=255)

                                                         aTTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCtattat                                                                                        >  2:6279581/1‑149 (MQ=255)

                                                         aTTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCtattat                                                                                        >  1:7265158/1‑149 (MQ=255)

                                                          ttATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTattatt                                                                                       >  1:4912749/1‑149 (MQ=255)

                                                            ataCAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTaa                                                                                                                                                <  1:4486569/90‑1 (MQ=255)

                                                             taCAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGa                                                                                    >  1:4101364/1‑149 (MQ=255)

                                                             taCAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGa                                                                                    <  1:5541716/149‑1 (MQ=255)

                                                              aCAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGaa                                                                                   >  1:6741454/1‑149 (MQ=255)

                                                              aCAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGaa                                                                                   <  2:4781528/149‑1 (MQ=255)

                                                                  aaaaaaCACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAAcgcg                                                                               <  2:4327781/149‑1 (MQ=255)

                                                                  aaaaaaCACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAAcgcg                                                                               <  2:5497367/149‑1 (MQ=255)

                                                                     aaaCACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGgtg                                                                            >  2:2811925/1‑149 (MQ=255)

                                                                     aaaCACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGgtg                                                                            <  2:7070227/149‑1 (MQ=255)

                                                                      aaCACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGgtgt                                                                           <  2:3214600/149‑1 (MQ=255)

                                                                       acacTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTa                                                                          <  2:7907748/149‑1 (MQ=255)

                                                                       acacTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTa                                                                          >  1:3030496/1‑149 (MQ=255)

                                                                        cacTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTag                                                                         >  2:4174693/1‑149 (MQ=255)

                                                                        cacTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTag                                                                         >  2:9529321/1‑149 (MQ=255)

                                                                         acTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGCAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTTAATCTCTATTATTAGAACGCGGGGTaga                                                                        >  2:510127/1‑149 (MQ=255)

                                                                          cTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTagag                                                                       <  1:9549425/149‑1 (MQ=255)

                                                                           tAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATAAAAAGAGATAAAAGATTAGAAATTACTAAGGAAATTGTGACGAATAATAAAATATTGACACATGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTagatg                                                                      >  2:6944215/1‑147 (MQ=255)

                                                                            aaTTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGt                                                                     <  2:4672320/149‑1 (MQ=255)

                                                                               tttGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAac                                                                  <  1:6442729/149‑1 (MQ=255)

                                                                                ttGTCAATATATAGTCCATTATATGGTAGAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAaca                                                                 <  1:6256298/149‑1 (MQ=255)

                                                                                 tGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAacaa                                                                >  2:6558944/1‑148 (MQ=255)

                                                                                  gTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAAcaca                                                               <  1:6049921/149‑1 (MQ=255)

                                                                                   tCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACaa                                                              <  2:3590336/149‑1 (MQ=255)

                                                                                         tataGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACt                                                        <  1:7593119/149‑1 (MQ=255)

                                                                                         tataGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACt                                                        <  1:3717955/149‑1 (MQ=255)

                                                                                           taGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACttt                                                      >  2:7185042/1‑149 (MQ=255)

                                                                                           taGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACttt                                                      >  2:1874211/1‑149 (MQ=255)

                                                                                           taGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACttt                                                      >  1:2055078/1‑149 (MQ=255)

                                                                                           taGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACttt                                                      >  2:8282807/1‑149 (MQ=255)

                                                                                              tCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATc                                                   >  2:4901054/1‑149 (MQ=255)

                                                                                                 aTTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCag                                                <  1:4222633/149‑1 (MQ=255)

                                                                                                 aTTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCag                                                <  1:3441154/149‑1 (MQ=255)

                                                                                                  ttATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAAGTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCaga                                               <  2:891443/149‑1 (MQ=255)

                                                                                                   tataTGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCagag                                              >  1:1502098/1‑149 (MQ=255)

                                                                                                   tataTGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCagag                                              <  1:1658015/149‑1 (MQ=255)

                                                                                                    atatGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAgaga                                             <  1:4005628/149‑1 (MQ=255)

                                                                                                    atatGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAgaga                                             <  1:5456378/149‑1 (MQ=255)

                                                                                                      atGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGata                                           <  1:748749/149‑1 (MQ=255)

                                                                                                         gtctaATTATGCTATGAAAAGAGATAAAAGATTAGAACTTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTa                                        <  2:5376010/146‑1 (MQ=255)

                                                                                                          tataATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAg                                       <  1:7964854/149‑1 (MQ=255)

                                                                                                          tataATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAg                                       <  1:816933/149‑1 (MQ=255)

                                                                                                          tataATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAg                                       <  1:8382399/149‑1 (MQ=255)

                                                                                                           gtaaagaTGTTATAAAAAGAGAGAAGCCGTTACATATTACTATAGGAATTGTGGCAAATAATAAGATTTTGTCTGTAGAAGAGATTCATACCCTGTTCTTAGAACGCGGTGTAGAGGTAACACACGCCACTTTATCCCTTGATATTAGa                                      <  2:446560/143‑1 (MQ=255)

                                                                                                           ataATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGa                                      >  1:1330441/1‑149 (MQ=255)

                                                                                                           ataATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGa                                      <  1:906557/149‑1 (MQ=255)

                                                                                                             aaTTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCAACTTTATCCAGAGATATTAGaaa                                    >  2:2586674/1‑149 (MQ=255)

                                                                                                              aTTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGaaaa                                   <  1:5706254/149‑1 (MQ=255)

                                                                                                               ttATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAAt                                  <  1:7771897/149‑1 (MQ=255)

                                                                                                                    cTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAat                             <  1:9329402/149‑1 (MQ=255)

                                                                                                                           aaGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATaa                      >  2:7604781/1‑149 (MQ=255)

                                                                                                                            agagATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATaaa                     >  2:1172050/1‑149 (MQ=255)

                                                                                                                            agagATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATaaa                     <  1:8753504/149‑1 (MQ=255)

                                                                                                                                 tAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAAc                >  2:2015485/1‑149 (MQ=255)

                                                                                                                                 tAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAAc                >  2:7436873/1‑149 (MQ=255)

                                                                                                                                  aaaaGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACg               <  2:670945/149‑1 (MQ=255)

                                                                                                                                  aaaaGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACg               >  2:8467389/1‑149 (MQ=255)

                                                                                                                                  aaaaGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACg               >  1:6561396/1‑149 (MQ=255)

                                                                                                                                   aaaGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAAACTCTATTATTAGAACGGGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATAGTAGAAAATTAAATATTATAAAAAAACgg              <  2:1785944/149‑1 (MQ=255)

                                                                                                                                        ttAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACGGGACaa         >  2:103969/1‑149 (MQ=255)

                                                                                                                                         aaGAAAATACTAAAAAAATTAGGACAAAAAATAAAATAGTGACTAAAGAAGAGATTCAATCTCTATTATAAAAACACGGTGTAGAAGTAAAAAAAGTCACTTTATCCAGAGATATTAGAAAATTAAATAATAAAAAAAAACGGGACaaa        <  2:6390774/148‑1 (MQ=255)

                                                                                                                                              attactAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACGGGACAAAGgaga   >  1:3587215/1‑149 (MQ=255)

ACCATCGATTGCAGCAGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTATTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACGGGACAAAGGAGAG  >  AM406671/2266578‑2266869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`