Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 1,933,214 2 bp→CG coding (224‑225/714 nt) atpB ← ATP synthase F0, A subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM4066711,933,2140GC99.9% 3844.0 / NA 922G75A (GGC→GCG) atpBATP synthase F0, A subunit
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (497/424);  total (498/424)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*AM4066711,933,2150CG99.9% 3815.5 / NA 922G75A (GGC→GCG) atpBATP synthase F0, A subunit
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (498/422);  total (498/424)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 922 total aligned reads displayed.

ACAAGAAGTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGGCCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGAAAACAAT  >  AM406671/1933071‑1933357
                                                                                                                                               ||                                                                                                                                              
  aagaagTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGc                                                                                                                                          <  1:5865823/149‑1 (MQ=255)
   agaagTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCg                                                                                                                                         >  1:754395/1‑149 (MQ=255)
    gaagTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCgt                                                                                                                                        >  1:3141051/1‑149 (MQ=255)
      agTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCgtgg                                                                                                                                      >  2:8502783/1‑149 (MQ=255)
      agTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCgtgg                                                                                                                                      <  2:5108816/149‑1 (MQ=255)
      agTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCgtgg                                                                                                                                      >  1:8584238/1‑149 (MQ=255)
       gTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCgtggt                                                                                                                                     <  2:1448889/149‑1 (MQ=255)
        tGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCgtggtg                                                                                                                                    >  2:2882114/1‑149 (MQ=255)
          cAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCt                                                                                                                                  <  2:1810369/149‑1 (MQ=255)
           aggtgtCAAATCGACTGTTGGATTTGCAGTGTGTGATTTCCAAAGGGGAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCtt                                                                                                                                 <  1:4402131/147‑1 (MQ=255)
                tCAAATCGACTGTTGGATTTGCAGTTCGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTNCATGAGCGCAAAGCGTGGTGCTTCATGa                                                                                                                            <  1:5377929/149‑1 (MQ=255)
                 cAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAg                                                                                                                           >  1:8504310/1‑149 (MQ=255)
                  aaaTCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAgg                                                                                                                          <  2:739893/149‑1 (MQ=255)
                    aTCGACTGTTGAATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGc                                                                                                                        <  2:4220639/149‑1 (MQ=255)
                     tCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGcc                                                                                                                       >  1:6856293/1‑149 (MQ=255)
                      cGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCg                                                                                                                      <  1:1444109/149‑1 (MQ=255)
                       gACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGa                                                                                                                     <  1:7214508/149‑1 (MQ=255)
                         cTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACg                                                                                                                   <  2:7920745/149‑1 (MQ=255)
                         cTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACg                                                                                                                   >  1:1923064/1‑149 (MQ=255)
                         cTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCAAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACg                                                                                                                   >  2:1684583/1‑149 (MQ=255)
                           gTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGtt                                                                                                                 <  1:6682148/149‑1 (MQ=255)
                           gTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGtt                                                                                                                 <  2:6580587/149‑1 (MQ=255)
                             tGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTAt                                                                                                               <  2:6006952/149‑1 (MQ=255)
                               gATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCt                                                                                                             <  1:1627684/149‑1 (MQ=255)
                                   tGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTgg                                                                                                         <  2:2110261/149‑1 (MQ=255)
                                    gCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGa                                                                                                        <  2:3022465/149‑1 (MQ=255)
                                    gCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGa                                                                                                        <  2:9278862/149‑1 (MQ=255)
                                       gTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAAc                                                                                                     >  1:8738677/1‑149 (MQ=255)
                                        ttGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACt                                                                                                    >  2:6113564/1‑149 (MQ=255)
                                         tGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGATGTTATCTTTGGAAACTc                                                                                                   <  2:4953768/149‑1 (MQ=255)
                                         tGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTc                                                                                                   >  1:3744045/1‑149 (MQ=255)
                                          ggTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTcc                                                                                                  >  2:3853111/1‑149 (MQ=255)
                                           gTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCa                                                                                                 >  2:8410365/1‑149 (MQ=255)
                                            tGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCAt                                                                                                >  1:6534022/1‑149 (MQ=255)
                                            tGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCAt                                                                                                >  1:2870747/1‑149 (MQ=255)
                                              aTTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTTATGAGGGCCGACGTTATCTTTGGAAACTCCATTg                                                                                              >  2:2673399/1‑149 (MQ=255)
                                                     aaGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAAt                                                                                       >  1:9051451/1‑149 (MQ=255)
                                                      agagAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATc                                                                                      >  1:5509216/1‑149 (MQ=255)
                                                       gagaAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATca                                                                                     <  1:8300907/149‑1 (MQ=255)
                                                         gaAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATcaac                                                                                   >  2:3303852/1‑149 (MQ=255)
                                                           aaCATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATcaacaa                                                                                 <  1:9504733/149‑1 (MQ=255)
                                                             gaTCACCGGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCACTAAGAAGGAAAGAAAGGAGGGTAAAGTTAATGAGCGCAAAGCGTGGTGGTTCATGAGGGCCGCCGTTATCTTTGGAAACTCTACTGACAAAATCAACAAcc                                                                               >  2:4321638/2‑149 (MQ=255)
                                                              aTCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAAccc                                                                              <  1:6206982/149‑1 (MQ=255)
                                                               tCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCa                                                                             <  2:2759329/149‑1 (MQ=255)
                                                                 aCCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACt                                                                           <  2:718580/149‑1 (MQ=255)
                                                                 aCCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACt                                                                           >  2:7725396/1‑149 (MQ=255)
                                                                  ccTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTc                                                                          >  1:4977427/1‑149 (MQ=255)
                                                                   cTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCa                                                                         <  1:733354/149‑1 (MQ=255)
                                                                          aCTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTAAAGAACAt                                                                  >  2:3679302/1‑149 (MQ=255)
                                                                           cTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACAtt                                                                 <  2:1121787/149‑1 (MQ=255)
                                                                           cTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACAtt                                                                 <  2:6774349/149‑1 (MQ=255)
                                                                           cTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACAtt                                                                 <  1:1029229/149‑1 (MQ=255)
                                                                             aaTTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACAtttt                                                               <  1:6033172/149‑1 (MQ=255)
                                                                             aaTTTTAGTTACTAGCACAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAAAAtttt                                                               >  2:2325040/1‑149 (MQ=255)
                                                                                tttAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGtt                                                            >  1:3551507/1‑149 (MQ=255)
                                                                                 ttAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGttt                                                           >  2:6247513/1‑149 (MQ=255)
                                                                                   aGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTAc                                                         <  2:4830322/149‑1 (MQ=255)
                                                                                    gTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGTGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTAcc                                                        >  1:4004316/1‑149 (MQ=255)
                                                                                     ttACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCt                                                       >  1:4265040/1‑149 (MQ=255)
                                                                                      tACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCtt                                                      <  1:9251549/149‑1 (MQ=255)
                                                                                       aCTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCttt                                                     >  1:2541108/1‑149 (MQ=255)
                                                                                        cTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTg                                                    <  2:754395/149‑1 (MQ=255)
                                                                                        cTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTg                                                    >  2:9435098/1‑149 (MQ=255)
                                                                                        cTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTg                                                    >  1:5111204/1‑149 (MQ=255)
                                                                                         tAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACACCCCACTCAAGAACATTTTGTTTACATTCgg                                                   >  2:25390/1‑149 (MQ=255)
                                                                                         tAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAACGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTgg                                                   >  1:5675691/1‑149 (MQ=255)
                                                                                          aGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTggg                                                  <  2:8124520/149‑1 (MQ=255)
                                                                                           gCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGc                                                 <  2:6350260/149‑1 (MQ=255)
                                                                                              cAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTg                                              <  1:2181902/149‑1 (MQ=255)
                                                                                              cAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTg                                              <  2:6865411/149‑1 (MQ=255)
                                                                                               aaTGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGa                                             >  1:7925234/1‑149 (MQ=255)
                                                                                               aaTGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGa                                             <  2:3492819/149‑1 (MQ=255)
                                                                                                aTGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAg                                            >  1:480132/1‑149 (MQ=255)
                                                                                                 tGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGc                                           >  2:5314993/1‑149 (MQ=255)
                                                                                                    aGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGc                                        <  2:5704628/149‑1 (MQ=255)
                                                                                                      ttGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCat                                      >  1:3307994/1‑149 (MQ=255)
                                                                                                       tGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCata                                     <  1:4140960/149‑1 (MQ=255)
                                                                                                          cAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCatatgg                                  <  2:8606181/149‑3 (MQ=255)
                                                                                                           aaTAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGc                                 <  2:5536023/149‑1 (MQ=255)
                                                                                                             tAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCgg                               >  2:4060780/1‑149 (MQ=255)
                                                                                                                   ggAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGcc                         >  2:8032864/1‑149 (MQ=255)
                                                                                                                    gaaagaaaAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGccc                        <  1:9435098/149‑1 (MQ=255)
                                                                                                                     aaagaaaagaGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGc                                           >  2:8725379/1‑129 (MQ=255)
                                                                                                                     aaagaaaagaGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCa                       >  1:7038802/1‑149 (MQ=255)
                                                                                                                       agaaaagaGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGa                     <  1:2616210/149‑1 (MQ=255)
                                                                                                                         aaaagaGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGTAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAAt                   >  1:5768682/1‑149 (MQ=255)
                                                                                                                         aaaagaGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAAt                   >  1:8072090/1‑149 (MQ=255)
                                                                                                                          aaagaGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATa                  <  2:1271399/149‑1 (MQ=255)
                                                                                                                            agagGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATAcc                <  2:4647564/149‑1 (MQ=255)
                                                                                                                             gagGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCa               <  1:2306810/149‑1 (MQ=255)
                                                                                                                                 gTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGAcc           <  1:4535528/149‑1 (MQ=255)
                                                                                                                                  tAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCg          <  2:7001162/149‑1 (MQ=255)
                                                                                                                                   aaaGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGa         <  1:1761052/149‑1 (MQ=255)
                                                                                                                                   aaaGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGa         >  1:118860/1‑149 (MQ=255)
                                                                                                                                   aaaGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGa         >  1:775623/1‑149 (MQ=255)
                                                                                                                                   aaaGTTCATGAGCGCAAAGCGCGGTGCTTCATGAGGGCCGACGTTATCTTTGGCAACTCCATTGACAACATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGCGCTGCATCTTGCGGCTCCCCCAGAATACCAGACCgc         >  2:8957852/1‑148 (MQ=255)
                                                                                                                                     aGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGaaa       >  2:9119335/1‑149 (MQ=255)
                                                                                                                                       ttCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGaaaaa     >  2:4515524/1‑148 (MQ=255)
                                                                                                                                       ttCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGAAAAc     <  2:8248764/149‑1 (MQ=255)
                                                                                                                                        tCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGGTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCTGGAATACCCGACCGGAAACa    >  2:1968402/1‑149 (MQ=255)
                                                                                                                                               ||                                                                                                                                              
ACAAGAAGTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGGCCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGAAAACAAT  >  AM406671/1933071‑1933357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑