BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,916,379	Δ1 bp	pseudogene (355/598 nt)	llmg_pseudo_59 ←	pseudogene

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,916,379	0	T	.	99.6%	5569.3 / 9.9	1167	pseudogene (355/598 nt)	llmg_pseudo_59	pseudogene
Reads supporting (aligned to +/- strand): ref base T (4/1); new base . (574/586); total (580/587)
Fisher's exact test for biased strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1184 total aligned reads displayed.

CGCGGCTTCAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATATGGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCATTTTTAGATG  >  AM406671/1916235‑1916522

  cgGCTTCAATATCCAATTTTCGGTCACCGATATAATAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGAATTGGTTTTCTTTTAAAATTATTTTCAGAGGTAAAAAAAAAAAAAAAATA‑Ggaagaa                                                                                                                                          <  2:11198674/149‑1 (MQ=255)

     cTTCAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAAtt                                                                                                                                       >  2:1417785/1‑149 (MQ=255)

      ttCAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAATAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTc                                                                                                                                      >  2:7182851/1‑149 (MQ=255)

       tCAATATCCAATTTTCGGTCACCGATATAGTAGGATTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTcc                                                                                                                                     <  2:115905/149‑1 (MQ=255)

        cAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCa                                                                                                                                    >  1:69374/1‑149 (MQ=255)

         aaTATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCaa                                                                                                                                   <  2:10102935/149‑1 (MQ=255)

         aaTATCCAATTTTCGGTCACCGATATAGAAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCGAAAAAAT‑GGGAAGAAATTCCaa                                                                                                                                   >  1:2492955/1‑149 (MQ=255)

          atatCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAg                                                                                                                                  >  1:1017418/1‑149 (MQ=255)

          atatCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAg                                                                                                                                  <  1:9989921/149‑1 (MQ=255)

           tatCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAgt                                                                                                                                 <  2:9051947/149‑1 (MQ=255)

                 aTTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTg                                                                                                                           >  1:3721433/1‑149 (MQ=255)

                 aTTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTg                                                                                                                           <  2:5956927/149‑1 (MQ=255)

                  ttttCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGa                                                                                                                          <  2:8328537/149‑1 (MQ=255)

                     tCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTa                                                                                                                       <  1:8466610/149‑1 (MQ=255)

                       ggTCACCGATATAGTAGGTATCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTaaa                                                                                                                     <  1:5622123/149‑1 (MQ=255)

                        atCACCGATATAGGAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTACAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAAt                                                                                                                    <  2:850315/148‑1 (MQ=255)

                         tCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATc                                                                                                                   >  2:3932654/1‑149 (MQ=255)

                            ccGATATAGTAGTTTTCATCAGTCTTCAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGCGGTAAAAAAAAAAAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCaaa                                                                                                                <  2:4544506/149‑1 (MQ=255)

                               atatAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGc                                                                                                             >  1:10579061/1‑149 (MQ=255)

                                tataGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCa                                                                                                            >  1:2085081/1‑149 (MQ=255)

                                   agtagGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCAttt                                                                                                         >  1:594605/1‑149 (MQ=255)

                                     tagGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCAttttt                                                                                                       >  2:9437069/1‑149 (MQ=255)

                                          ttCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACCACTTCCAAAAAATA‑GTAAGAAATTCCAAGTTGTTTGAGTAAATCAAACGCATTTTTACCtt                                                                                                  >  2:4437956/1‑149 (MQ=255)

                                               caGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGa                                                                                             <  2:7641632/149‑1 (MQ=255)

                                               caGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGa                                                                                             >  1:5216674/1‑149 (MQ=255)

                                                aGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAg                                                                                            >  1:7745787/1‑149 (MQ=255)

                                                  tCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGta                                                                                          <  1:8607000/149‑1 (MQ=255)

                                                  tCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGta                                                                                          <  1:10005666/149‑1 (MQ=255)

                                                    tttAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGtata                                                                                        <  1:7943478/149‑1 (MQ=255)

                                                    tttAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGtata                                                                                        >  1:10386404/1‑149 (MQ=255)

                                                     ttAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATaa                                                                                       >  2:6876234/1‑149 (MQ=255)

                                                     ttAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATaa                                                                                       <  1:1495272/149‑1 (MQ=255)

                                                      tAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATaaa                                                                                      <  1:9008730/149‑1 (MQ=255)

                                                      tAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATaaa                                                                                      >  1:7131177/1‑149 (MQ=255)

                                                        aaTTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATg                                                                                    >  1:6770656/1‑149 (MQ=255)

                                                         aTTATACTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCGTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAATATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTCCCTTTGTGAGTATAAATGa                                                                                   >  2:5548226/1‑149 (MQ=255)

                                                          atatataTTTTAACTAAGAAATTGATTGCATCAGTATTAGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGTAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCATATATGAGTATAAATGaa                                                                                  >  2:4750927/2‑149 (MQ=255)

                                                            atatTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGt                                                                                <  1:2715039/149‑1 (MQ=255)

                                                                  tttAACTAAGTAATTGATTGCCTCAACAATTGGTTTTCATTTAAAATTATTTTCAGAGGTAAAAAAAAAAAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGaa                                                                          <  2:10373986/149‑1 (MQ=255)

                                                                    tAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATg                                                                        <  2:703214/149‑1 (MQ=255)

                                                                      aCTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGtt                                                                      <  1:9292656/149‑1 (MQ=255)

                                                                       cTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTtgtg                                                                                              <  1:11243632/124‑1 (MQ=255)

                                                                          aGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTTCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCt                                                                  >  2:6426832/1‑149 (MQ=255)

                                                                           gTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCtt                                                                 >  1:3094198/1‑149 (MQ=255)

                                                                           gTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTACAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCtt                                                                 >  1:7298145/1‑149 (MQ=255)

                                                                            tAATTGATTGCCTCAGGATTTGGTCTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGAATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCttt                                                                >  1:4463970/1‑149 (MQ=255)

                                                                              attgattgCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGtttttttttt                                                              >  2:2678005/1‑149 (MQ=255)

                                                                               ttgattgCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTg                                                             >  2:1655470/1‑149 (MQ=255)

                                                                                tgattgCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGa                                                            <  1:9794220/149‑1 (MQ=255)

                                                                                 gattgCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAg                                                           <  1:1366665/149‑1 (MQ=255)

                                                                                 gattgCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAg                                                           <  2:2888855/149‑1 (MQ=255)

                                                                                  attgCCTCAGGATTTGGTTTTCTTTTAANATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATAAAGTTTTGAATGTTTTCTTTTTGAGt                                                          >  1:6364556/1‑149 (MQ=255)

                                                                                  attgCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGt                                                          >  2:792302/1‑149 (MQ=255)

                                                                                     gCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATCAATGAAGTTTTGAATGTTTTCTTTTTGAGtcca                                                       >  2:2527274/1‑149 (MQ=255)

                                                                                      ccTCAGGATTTTGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGtccaa                                                      <  2:1837683/149‑1 (MQ=255)

                                                                                      ccTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGtccaa                                                      >  2:9740299/1‑149 (MQ=255)

                                                                                       cTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGtccaat                                                     <  1:10727572/149‑1 (MQ=255)

                                                                                        tCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGtccaatc                                                    >  2:9216546/1‑149 (MQ=255)

                                                                                         cAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGtccaatcc                                                   <  1:2994691/149‑1 (MQ=255)

                                                                                          aGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAATAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGtctaatcta                                                  >  2:5238358/1‑147 (MQ=255)

                                                                                             aTTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCataa                                               >  2:3697718/1‑149 (MQ=255)

                                                                                              tttGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAAc                                              >  1:5380441/1‑149 (MQ=255)

                                                                                                tGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAActt                                            <  1:4797709/149‑1 (MQ=255)

                                                                                                  gTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAActtct                                          <  1:6162734/149‑1 (MQ=255)

                                                                                                   ttttCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAActtctt                                         <  2:568250/149‑1 (MQ=255)

                                                                                                    tttCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCttt                                        <  1:1731567/149‑1 (MQ=255)

                                                                                                      tCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGACATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATATATGAAGTTTTGAATGTTTTCTTTGTTAGTCCAATCCAAAACTTCTTTAg                                      <  2:6227406/149‑1 (MQ=255)

                                                                                                         tttAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCAc                                   <  1:3303011/149‑1 (MQ=255)

                                                                                                            aaaaTTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTa                                <  1:4603463/149‑1 (MQ=255)

                                                                                                            aaaaTTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTa                                >  1:118444/1‑149 (MQ=255)

                                                                                                                ttattTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCAt                            >  1:4225142/1‑149 (MQ=255)

                                                                                                                  attCTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTa                          <  2:8803278/149‑1 (MQ=255)

                                                                                                                   ttttCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTTCAATCCAAAACTTCTTTAGCACCTACCATTaa                         >  2:10656513/1‑149 (MQ=255)

                                                                                                                     ttCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGCGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAatt                       >  2:4122968/1‑149 (MQ=255)

                                                                                                                        agagGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAatttta                    >  1:3958802/1‑149 (MQ=255)

                                                                                                                         gagGTAACAACTTCCAAATAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATTTTTTCTTTTTGAGTCCAATCCAAATCTTCTTTAGCACCTACCATTAattttat                   <  2:6659802/149‑1 (MQ=255)

                                                                                                                         gagGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAattttat                   >  2:9572778/1‑149 (MQ=255)

                                                                                                                         gagGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAattttat                   <  2:3198243/149‑1 (MQ=255)

                                                                                                                          agGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCTTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTGattttatt                  >  1:6581471/1‑149 (MQ=255)

                                                                                                                          agGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTTATTCCAATCAAAAACTTTTTTATAACCTACCATTAattttatt                  >  2:4940415/1‑149 (MQ=255)

                                                                                                                          agGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAattttatt                  >  1:9601484/1‑149 (MQ=255)

                                                                                                                           ggTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAattttattt                 >  1:5064821/1‑149 (MQ=255)

                                                                                                                            gTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAattttatttt                >  2:10810936/1‑149 (MQ=255)

                                                                                                                            gTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAattttatttt                >  2:3062632/1‑149 (MQ=255)

                                                                                                                            gTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAattttatttt                <  1:3561054/149‑1 (MQ=255)

                                                                                                                             tAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCACTCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTg               >  1:8265692/1‑149 (MQ=255)

                                                                                                                              tatatcTTACAAAAAATA‑GGAAGAAATTCTAAGTTGTTTGAGTATATCAAAGGAATTTTTGCCTTTGTGAGTATAAATTAAGTTTTTAATGTTTTCTTTTTTATTCCAATCCAAAACGTGTTTAGAACCTATCATTAATTTTAtgttgt              <  2:2141854/144‑1 (MQ=255)

                                                                                                                              aacaacTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGt              >  1:5836639/1‑149 (MQ=255)

                                                                                                                                caacTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCa            <  1:11030026/149‑1 (MQ=255)

                                                                                                                                caacTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCa            >  1:8116961/1‑149 (MQ=255)

                                                                                                                                caacTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCa            >  1:7616173/1‑149 (MQ=255)

                                                                                                                                caacTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCa            >  1:4966437/1‑149 (MQ=255)

                                                                                                                                 aacTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCAt           >  1:10834723/1‑149 (MQ=255)

                                                                                                                                   cTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCAttt         <  2:5115571/149‑1 (MQ=255)

                                                                                                                                   cTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCAttt         >  1:3784428/1‑149 (MQ=255)

                                                                                                                                       cAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCATTTTTAg     >  1:2299720/1‑149 (MQ=255)

                                                                                                                                         aaaaaTA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCATTTTTAGAt   <  2:7347338/149‑1 (MQ=255)

                                                                                                                                         aaaaaTA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCATTTTTAGAt   <  1:8852457/149‑1 (MQ=255)

                                                                                                                                         aaaaaTA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCATTTTTAGAt   <  2:3476811/149‑1 (MQ=255)

                                                                                                                                          aaaaTA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCATTTTTAGATg  <  2:11007262/149‑1 (MQ=255)

CGCGGCTTCAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATATGGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCATTTTTAGATG  >  AM406671/1916235‑1916522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`