BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,921,639	Δ1 bp	intergenic (‑129/‑256)	llmg_1941 ← / → glnP	conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,921,636	0	T	.	99.6%	5129.0 / 12.2	1081	intergenic (‑126/‑259)	llmg_1941/glnP	conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein
Reads supporting (aligned to +/- strand): ref base T (2/0); major base . (573/498); minor base A (3/1); total (582/499)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1168 total aligned reads displayed.

AAAAATCGAAAGTTGTACAAACATAATATCCCTTTTCAAAAGTTTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAACTTTTCTTATATTTTATTCAGGAGAACTCTTCAAATTTCAACAAATTTCAAAATGTCAGGAAAACCTAAACATTCAACTTGATTTCTAGAACAATTTTTGTGATATAATTTTTCCCAGAAATAAAATAAACTGAGCTTGTCTTCCTAAATATAGGGAAA  >  AM406671/1921487‑1921793

aaaaaTCGAAAGTTGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAACt                                                                                                                                                               <  2:366068/149‑1 (MQ=255)

aaaaaTAGAAAGTTATACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAAAACATAAATATCTCTATCAAGCACTCGTTAAAAAAAAAAAAAAAAAGGACTTTTTGACAATATAAACt                                                                                                                                                               <  2:2084500/149‑1 (MQ=255)

 aaaaTCGAAAGTTGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAACtt                                                                                                                                                              <  1:5880198/149‑1 (MQ=255)

 aaaaTCGAAAGTTGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAACtt                                                                                                                                                              >  2:518065/1‑149 (MQ=255)

  aaaTCGAAAGTTGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAACttt                                                                                                                                                             <  1:7233443/149‑1 (MQ=255)

   aaTCGAAAGTTGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTATACAACAAAAAGGACTTTTTGACAATATAAACtttc                                                                                                                                                            <  1:1298106/149‑2 (MQ=255)

     tCGAAAGTTGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAACtttctt                                                                                                                                                          >  1:10221870/1‑146 (MQ=255)

     tCGAAAGTTGTACAAACATAATATTCCTGTTCAAAAGTTT‑AAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAACtttctt                                                                                                                                                          <  1:8220451/149‑4 (MQ=255)

          aGTTGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATAtt                                                                                                                                                    <  2:722288/149‑1 (MQ=255)

           gTTGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATAttt                                                                                                                                                   >  2:10767018/1‑149 (MQ=255)

            ttGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATAtttt                                                                                                                                                  <  1:6467028/149‑1 (MQ=255)

            ttGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATAtttt                                                                                                                                                  <  1:10005297/149‑1 (MQ=255)

            ttGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATAtttt                                                                                                                                                  <  2:4923457/149‑1 (MQ=255)

            ttGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTACTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTAAAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATAtttt                                                                                                                                                  <  2:10410353/149‑1 (MQ=255)

             tGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTa                                                                                                                                                 >  2:11047717/1‑149 (MQ=255)

             tGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTa                                                                                                                                                 >  1:2739771/1‑149 (MQ=255)

              gTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTAt                                                                                                                                                >  2:7431538/1‑149 (MQ=255)

              gTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTAt                                                                                                                                                <  1:373840/149‑1 (MQ=255)

                aCAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTAtca                                                                                                                                              >  1:4644135/1‑147 (MQ=255)

                aCAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTAtca                                                                                                                                              <  2:1540123/149‑3 (MQ=255)

                aCAAACAGAATATTCCTTTCCAAAAG‑TTAAAAATTCTTACCGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCATTTCTACAACAAAAAGGACTTTTTGACGGTATAAAC‑TTTCTTATATTTTAtca                                                                                                                                              <  2:6221541/149‑3 (MQ=255)

                   aaCATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTAtcagag                                                                                                                                           >  1:7615912/1‑144 (MQ=255)

                    aCATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTAtcagaga                                                                                                                                          <  2:8135547/149‑7 (MQ=255)

                          tattccTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAActctc                                                                                                                                  >  1:10646704/5‑148 (MQ=255)

                          tatTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAActctc                                                                                                                                  <  1:2547532/149‑2 (MQ=255)

                            ttccTTTTCAAAAG‑TTAAAAATTCTTACTGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAActctcaa                                                                                                                                <  1:7877449/147‑4 (MQ=255)

                             tccTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAActctcaaa                                                                                                                               >  2:4033304/2‑145 (MQ=255)

                              ccTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑Tcaaat                                                                                                                             <  1:1805953/149‑1 (MQ=255)

                                ttttCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑Tcaaattt                                                                                                                           <  2:5567304/149‑1 (MQ=255)

                                  ttCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑Tcaaatttca                                                                                                                         >  2:3788418/1‑149 (MQ=255)

                                  ttCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑Tcaaatttca                                                                                                                         >  2:4315994/1‑149 (MQ=255)

                                  ttCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑Tcaaatttca                                                                                                                         >  1:3565539/1‑149 (MQ=255)

                                        aagttAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑tcaaatttc‑acaaat                                                                                                                   >  1:9228163/4‑149 (MQ=255)

                                           ttAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑tcaaatttc‑acaaattca                                                                                                                <  1:6777762/149‑3 (MQ=255)

                                            tAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑tcaaatttc‑acaaattcaa                                                                                                               <  2:1150384/149‑4 (MQ=255)

                                            tAAAAATTCTTACAGTAGTTCTGTTTACTGAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑tcaaatttc‑acaaattcaa                                                                                                               <  1:140839/149‑4 (MQ=255)

                                                aaTTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATATTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATg                                                                                                          >  2:7922147/1‑149 (MQ=255)

                                                 aTTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGt                                                                                                         >  2:5376196/1‑149 (MQ=255)

                                                  ttCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTc                                                                                                        >  1:9693726/1‑149 (MQ=255)

                                                  ttCTGACAGTAGTTCTTGTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTc                                                                                                        <  2:1861664/149‑1 (MQ=255)

                                                   tCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCa                                                                                                       <  2:168610/149‑1 (MQ=255)

                                                   tCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCa                                                                                                       >  1:8906160/1‑149 (MQ=255)

                                                     ttACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑CCAAA‑TTCACAATGTCAga                                                                                                     >  2:301567/1‑148 (MQ=255)

                                                     ttACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCAga                                                                                                     <  1:2947607/149‑2 (MQ=255)

                                                          gtagtTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTGCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑AC‑AACTTCAAAATGTCA‑GAAAAcc                                                                                               >  1:5398100/1‑149 (MQ=255)

                                                           tagtTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCt                                                                                              <  2:10132190/149‑1 (MQ=255)

                                                               tCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCTaaca                                                                                          <  2:2354847/149‑3 (MQ=255)

                                                                cTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCTaacat                                                                                         <  1:11191165/149‑4 (MQ=255)

                                                                 tGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCTaacatc                                                                                        >  1:19917/1‑145 (MQ=255)

                                                                    ttACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCTaacatcaac                                                                                     <  2:6643513/149‑8 (MQ=255)

                                                                         tAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGAt                                                                              >  1:5693358/1‑149 (MQ=255)

                                                                           aaTAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGAttc                                                                            >  1:10374790/1‑148 (MQ=255)

                                                                            aTAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGAttct                                                                           >  2:950202/1‑147 (MQ=255)

                                                                             tAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGAttcta                                                                          <  1:1562616/149‑4 (MQ=255)

                                                                             tAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGAttcta                                                                          >  2:3338963/1‑146 (MQ=255)

                                                                              aGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGAttctag                                                                         <  1:2152939/149‑5 (MQ=255)

                                                                                aaTACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAGac                                                                      <  2:9807912/149‑2 (MQ=255)

                                                                                aaTACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAGac                                                                      <  1:7431538/149‑2 (MQ=255)

                                                                                 aTACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAGaca                                                                     <  2:7776794/149‑3 (MQ=255)

                                                                                  tACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTg                                                                                >  1:7046645/1‑138 (MQ=255)

                                                                                  tACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAGacaa                                                                    <  1:4150453/149‑4 (MQ=255)

                                                                                     aTAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAAttt                                                                <  1:9100192/149‑1 (MQ=255)

                                                                                     aTAAATATCTCTATCAAGCACTCGTTCAATTATACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAAttt                                                                <  2:2500094/149‑1 (MQ=255)

                                                                                      tAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAAtttt                                                               >  2:5881935/1‑149 (MQ=255)

                                                                                       aaaTATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAAttttt                                                              <  2:4644135/149‑1 (MQ=255)

                                                                                       aaaTATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAAttttt                                                              >  1:5524906/1‑149 (MQ=255)

                                                                                         atatCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTtgt                                                            <  1:8471045/149‑1 (MQ=255)

                                                                                               ctATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGAtata                                                      >  1:9373160/1‑149 (MQ=255)

                                                                                               ctATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGAtata                                                      >  2:2665064/1‑149 (MQ=255)

                                                                                               ctATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGAtata                                                      >  2:5706669/1‑149 (MQ=255)

                                                                                                 aTCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAt                                                    <  2:8587198/149‑1 (MQ=255)

                                                                                                   cAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAttt                                                  <  2:713686/149‑1 (MQ=255)

                                                                                                    aaGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAtttt                                                 <  2:2862231/149‑1 (MQ=255)

                                                                                                    aaGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAtttt                                                 <  1:10505627/149‑1 (MQ=255)

                                                                                                       cACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAATTTTccc                                              >  1:10831381/1‑149 (MQ=255)

                                                                                                         cTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAttttccaag                                            <  2:1301145/149‑6 (MQ=255)

                                                                                                         cTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAg                                           >  1:3340652/1‑149 (MQ=255)

                                                                                                          tCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAGa                                          >  2:6859824/1‑149 (MQ=255)

                                                                                                          tCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAGa                                          >  2:8828304/1‑149 (MQ=255)

                                                                                                             ttCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAGaata                                       <  1:8151609/149‑3 (MQ=255)

                                                                                                                 aTTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAg‑aataaaat                                  <  1:2355672/149‑1 (MQ=255)

                                                                                                                  ttttACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAg‑aataaaata                                 <  2:10633595/149‑1 (MQ=255)

                                                                                                                  ttttACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAg‑aataaaata                                 <  2:4205719/149‑1 (MQ=255)

                                                                                                                      acaacaAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACt                             >  2:8319034/1‑149 (MQ=255)

                                                                                                                          caaAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGc                         >  1:8027648/1‑149 (MQ=255)

                                                                                                                              aaGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGt                     <  1:4854778/149‑1 (MQ=255)

                                                                                                                              aaGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGt                     >  2:6449959/1‑149 (MQ=255)

                                                                                                                               aGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTc                    >  1:6050718/1‑149 (MQ=255)

                                                                                                                                ggACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTCt                   >  1:3958798/1‑149 (MQ=255)

                                                                                                                                ggACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTCt                   >  2:9125056/1‑149 (MQ=255)

                                                                                                                                  aCTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTCtcc                 >  1:8698417/1‑147 (MQ=255)

                                                                                                                                  aCTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTCtcc                 >  1:2239614/1‑147 (MQ=255)

                                                                                                                                  aCTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTCtcc                 <  2:9381434/149‑3 (MQ=255)

                                                                                                                                     ttttGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTaa             <  1:9990085/149‑1 (MQ=255)

                                                                                                                                       ttGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAat           <  1:6281539/149‑1 (MQ=255)

                                                                                                                                           cAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAg       >  1:3181849/1‑149 (MQ=255)

                                                                                                                                            aaTATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAgg      <  1:4513932/149‑1 (MQ=255)

                                                                                                                                               ataAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAggaaa   >  1:9814632/1‑146 (MQ=255)

                                                                                                                                               ataAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TGCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAGGaaa   >  2:10939807/1‑149 (MQ=255)

                                                                                                                                                taAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAGGaaac  >  1:5161848/1‑148 (MQ=255)

AAAAATCGAAAGTTGTACAAACATAATATCCCTTTTCAAAAGTTTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAACTTTTCTTATATTTTATTCAGGAGAACTCTTCAAATTTCAACAAATTTCAAAATGTCAGGAAAACCTAAACATTCAACTTGATTTCTAGAACAATTTTTGTGATATAATTTTTCCCAGAAATAAAATAAACTGAGCTTGTCTTCCTAAATATAGGGAAA  >  AM406671/1921487‑1921793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`