BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,921,777	Δ1 bp	intergenic (‑267/‑118)	llmg_1941 ← / → glnP	conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,921,776	0	T	.	99.8%	5798.6 / 4.0	1212	intergenic (‑266/‑119)	llmg_1941/glnP	conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein
Reads supporting (aligned to +/- strand): ref base T (1/0); major base . (533/673); minor base A (3/0); total (538/674)
Fisher's exact test for biased strand distribution p-value = 8.69e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1248 total aligned reads displayed.

GGACTTTTTGACAATATAAACTTTTCTTATATTTTATTCAGGAGAACTCTTCAAATTTCAACAAATTTCAAAATGTCAGGAAAACCTAAACATTCAACTTGATTTCTAGAACAATTTTTGTGATATAATTTTTCCCAGAAATAAAATAAACTGAGCTTGTCTTCCTAAATATAGGGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAGGAGACAAAATGAAGAAATTATTTTTCGCTCTGGCA  >  AM406671/1921615‑1921921

ggACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTCt                                                                                                                                                   >  1:5488830/1‑149 (MQ=255)

ggACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTCt                                                                                                                                                   >  2:9125056/1‑149 (MQ=255)

  aCTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTCtcc                                                                                                                                                 <  1:7766108/149‑3 (MQ=255)

   cTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTCtcct                                                                                                                                                >  1:5306951/1‑146 (MQ=255)

      tttGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTaaa                                                                                                                                            <  2:9000492/149‑1 (MQ=255)

           cAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAg                                                                                                                                       <  2:8548126/149‑1 (MQ=255)

           cAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAg                                                                                                                                       >  1:3181849/1‑149 (MQ=255)

            aaTATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAgg                                                                                                                                      <  1:4513932/149‑1 (MQ=255)

             atatAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAgga                                                                                                                                     >  1:7976407/1‑148 (MQ=255)

             atatAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAgga                                                                                                                                     <  2:2514297/149‑2 (MQ=255)

              tataAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAggaa                                                                                                                                    >  2:10423520/1‑147 (MQ=255)

              tataAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAggaa                                                                                                                                    <  1:5794023/149‑3 (MQ=255)

              tataAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAggaa                                                                                                                                    >  1:10416169/1‑147 (MQ=255)

               ataAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAggaaa                                                                                                                                   >  1:940576/1‑146 (MQ=255)

               ataAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAGGaaa                                                                                                                                   >  1:8143214/1‑149 (MQ=255)

                  aaactttCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑ACCA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACt                                                                                                                                <  2:6343009/145‑1 (MQ=255)

                     ctttCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTaaa                                                                                                                             <  1:6554398/148‑1 (MQ=255)

                      tttCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTaaaa                                                                                                                            <  1:6073406/149‑1 (MQ=255)

                       ttCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAg                                                                                                                           >  1:9505282/1‑149 (MQ=255)

                       ttCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAg                                                                                                                           >  1:691645/1‑149 (MQ=255)

                         cTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGaa                                                                                                                         >  1:3011125/1‑149 (MQ=255)

                          ttATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAAt                                                                                                                        >  1:1153596/1‑149 (MQ=255)

                            atatTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGt                                                                                                                      >  1:7347589/1‑149 (MQ=255)

                             tatTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGtt                                                                                                                     <  1:10753549/149‑1 (MQ=255)

                             tatTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGtt                                                                                                                     >  2:7437778/1‑149 (MQ=255)

                                  tttatcagagaACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTAtt                                                                                                                  <  1:3788418/142‑1 (MQ=255)

                                    tatcagagaACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTcc                                                                                                                >  2:9533982/6‑149 (MQ=255)

                                         gagaACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGAttt                                                                                                           <  2:5928267/149‑1 (MQ=255)

                                         gagaACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGAttt                                                                                                           <  1:3370998/149‑1 (MQ=255)

                                           gaACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGAttttt                                                                                                         <  1:5094261/149‑1 (MQ=255)

                                                 ctCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCa                                                                                                    <  1:4158803/148‑1 (MQ=255)

                                                 ctCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCa                                                                                                    >  2:6136402/2‑149 (MQ=255)

                                                  tCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCag                                                                                                   >  1:3918152/1‑149 (MQ=255)

                                                  tCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCag                                                                                                   <  1:9230308/149‑1 (MQ=255)

                                                     aatttc‑acaaa‑ttcaaaaTGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCagaag                                                                                                <  2:9832113/149‑1 (MQ=255)

                                                      atttc‑acaaa‑ttcaaaaTGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCagaaga                                                                                               >  2:1595786/1‑149 (MQ=255)

                                                      atttc‑acaaa‑ttcaaaaTGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCagaaga                                                                                               <  1:9046751/149‑1 (MQ=255)

                                                      atttc‑acaaa‑ttcaaaaTGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTACTCCGATTTTTATTCagaaga                                                                                               <  1:8417557/149‑1 (MQ=255)

                                                        tttcacaaa‑ttcaaaATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAgaagaa                                                                                              <  2:11320776/145‑1 (MQ=255)

                                                          tcacaaa‑ttcaaaATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACa                                                                                            <  2:3875680/147‑1 (MQ=255)

                                                              caaattcaaAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACAttt                                                                                         <  1:162569/145‑1 (MQ=255)

                                                               aaattcaaAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACAtttt                                                                                        <  2:10155069/146‑1 (MQ=255)

                                                               aaattcaaAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACAtttt                                                                                        >  1:11102681/4‑149 (MQ=255)

                                                                  ttcaaAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACAttttttt                                                                                     >  2:4034988/1‑149 (MQ=255)

                                                                     aaaaTGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAAt                                                                                  <  2:5706642/149‑1 (MQ=255)

                                                                     aaaaTGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAAt                                                                                  <  1:997198/149‑1 (MQ=255)

                                                                     aaaaTGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTAAGAAGAACATTTTTTTAAt                                                                                  >  1:5613954/1‑149 (MQ=255)

                                                                      aaaTGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAAtt                                                                                 <  2:2843041/149‑1 (MQ=255)

                                                                        aTGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTc                                                                               >  2:184680/1‑149 (MQ=255)

                                                                        aTGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTc                                                                               >  2:9377951/1‑149 (MQ=255)

                                                                        aTGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTc                                                                               <  2:426977/149‑1 (MQ=255)

                                                                         tGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCa                                                                              >  1:8615903/1‑149 (MQ=255)

                                                                           gtcagAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCAt                                                                             <  2:6862666/145‑1 (MQ=255)

                                                                            tcagAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCAtt                                                                            <  1:2573356/146‑1 (MQ=255)

                                                                             cagAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCAttt                                                                           <  1:4813757/147‑1 (MQ=255)

                                                                             cagAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCAttt                                                                           <  2:346165/147‑1 (MQ=255)

                                                                               gAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCAttttt                                                                         <  2:8115060/149‑1 (MQ=255)

                                                                               gAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCAttttt                                                                         <  2:1405503/149‑1 (MQ=255)

                                                                                aaaaCCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTACCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGATATTCCGATTTTTATTCAGAAGAACATTTTCTTAATTTCAtttttt                                                                        >  2:8787756/1‑149 (MQ=255)

                                                                                 aaaCCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCAttttttt                                                                       <  2:3191866/149‑1 (MQ=255)

                                                                                      cctaacatCAACTTGA‑TTCTAG‑ACAATTNTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTtata                                                                    >  1:6388764/8‑149 (MQ=255)

                                                                                       ctaacatCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTatat                                                                   >  1:7617432/7‑149 (MQ=255)

                                                                                       ctaacatCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTatat                                                                   >  1:619493/7‑149 (MQ=255)

                                                                                         aacatCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCTTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTAttttt                                                                 >  2:11013377/5‑148 (MQ=255)

                                                                                                      attCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTc                                                     >  1:2395765/2‑149 (MQ=255)

                                                                                                        tCTAG‑ACAATTTTTGTGTTATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCaa                                                   >  2:5082267/1‑149 (MQ=255)

                                                                                                            agaCAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAAtc                                                >  1:10943364/3‑149 (MQ=255)

                                                                                                               cAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCa                                             <  2:4422486/149‑1 (MQ=255)

                                                                                                                aaTTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCat                                            >  1:1823658/1‑149 (MQ=255)

                                                                                                                 aTTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCata                                           <  1:10638075/149‑1 (MQ=255)

                                                                                                                  tttttGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCatat                                          <  2:10309641/149‑1 (MQ=255)

                                                                                                                       gtgATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTCTTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAg                                     >  1:7144869/1‑149 (MQ=255)

                                                                                                                       gtgATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAg                                     >  2:7777350/1‑149 (MQ=255)

                                                                                                                       gtgATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAg                                     <  1:10179961/149‑1 (MQ=255)

                                                                                                                        tgATATAA‑TTTTCCAAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAgg                                    <  2:8457130/149‑1 (MQ=255)

                                                                                                                         gATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAGga                                   <  1:10024767/149‑1 (MQ=255)

                                                                                                                          atatAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCa                                             >  2:759582/1‑138 (MQ=255)

                                                                                                                          atatAA‑TTTTCCCAG‑AATAAAATAAACTGAGCATGT‑ATCCTTAATA‑AAGGAAACTAAAAGAATGTTATTCCGCTTTTTATTCAGAAGAACATTTATTTAATTGCATTTTTGTATTTTTAGGGATGATATAAACTCTTATATTCTAGgag                                  >  2:3728453/1‑149 (MQ=255)

                                                                                                                           tataA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAGgaga                                 >  2:9651724/1‑149 (MQ=255)

                                                                                                                           tataA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAGgaga                                 <  2:10620809/149‑1 (MQ=255)

                                                                                                                                 ttttCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAGGAGACaaaa                            <  1:1428219/149‑1 (MQ=255)

                                                                                                                                 ttttCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAGGAGACaaaa                            <  1:1983272/149‑1 (MQ=255)

                                                                                                                                   ttCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAGGAGACAAAATg                          <  1:2816254/149‑1 (MQ=255)

                                                                                                                                    tCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAGGAGACAAAATga                         <  1:4697007/149‑1 (MQ=255)

                                                                                                                                     cccAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAGGAGACAAAATgaa                        >  2:5379525/1‑149 (MQ=255)

                                                                                                                                       ccagaataaaataaACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAGGAGACAAAATgaag                       >  2:7918620/5‑149 (MQ=255)

                                                                                                                                         agaataaaataaACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAGGAGACAAAATgaagaa                     >  1:8790490/3‑149 (MQ=255)

                                                                                                                                           aataaaataaACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTTATTTTTTTATATTAAGGGATAATATCAAATCTCATATTCAAGGAGACAAAATGAAGAAat                   >  2:5502369/1‑149 (MQ=255)

                                                                                                                                            ataaaataaaCTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAGGAGACAAAATGAAGAAatt                  <  1:4340450/149‑1 (MQ=255)

                                                                                                                                             taaaataaaCTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAGGAGACAAAATGAAGAAatta                 <  2:2165717/149‑1 (MQ=255)

                                                                                                                                                aataaaCTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAATATTTTTTTAATTTTATTTTTTTATATTAAGGGATAATCTCAAAACTCATTTTCAAGGAGACAAAATGAAGAAATTAttt              >  2:7197781/1‑149 (MQ=255)

                                                                                                                                                aataaaCTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAGGAGACAAAATGAAGAAATTAttt              <  2:4576198/149‑1 (MQ=255)

                                                                                                                                                aataaaCTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAGGAGACAAAATGAAGAAATTAttt              <  1:2184006/149‑1 (MQ=255)

                                                                                                                                                     aCTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAGGAGACAAAATGAAGAAATTATTTTTCGc         <  2:2058389/149‑1 (MQ=255)

                                                                                                                                                       tGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAGGAGACAAAATGAAGAAATTATTTTTCGctc       <  1:3548255/149‑1 (MQ=255)

                                                                                                                                                       tGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAGGAGACAAAATGAAGAAATTATTTTTCGctc       >  2:2332547/1‑149 (MQ=255)

                                                                                                                                                         aGCTTGTC‑TCCTATATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATGTTTTTAATTTCATTTTTTTATATTAAGGGATATTCTCATATCTCGTATTCCAGGAGACAAAATGAAGCAATTATTTTTCGCTCTg     <  2:6516490/149‑1 (MQ=255)

                                                                                                                                                           cTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTTTTTTTTTTATCATTTTTTTAATTTCATTTTTTTATATTATTGTATAAGCTCAAATCTCATATTTAAGGATACAAAATTAAGACATTATTTTTCGCTCTTGc   >  2:4803490/1‑149 (MQ=255)

                                                                                                                                                            ttGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAGGAGACAAAATGAAGAAATTATTTTTCGCTCTGGCa  <  1:9794861/149‑1 (MQ=255)

                                                                                                                                                            ttGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAGGAGACAAAATGAAGAAATTATTTTTCGCTCTGGCa  <  2:10851495/149‑1 (MQ=255)

GGACTTTTTGACAATATAAACTTTTCTTATATTTTATTCAGGAGAACTCTTCAAATTTCAACAAATTTCAAAATGTCAGGAAAACCTAAACATTCAACTTGATTTCTAGAACAATTTTTGTGATATAATTTTTCCCAGAAATAAAATAAACTGAGCTTGTCTTCCTAAATATAGGGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAGGAGACAAAATGAAGAAATTATTTTTCGCTCTGGCA  >  AM406671/1921615‑1921921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`