BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,210,283	(A)_5→6	pseudogene (515/1046 nt)	llmg_pseudo_39 ←	pseudogene

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,210,278	1	.	A	98.1%	3518.6 / 39.9	903	pseudogene (520/1046 nt)	llmg_pseudo_39	pseudogene
Reads supporting (aligned to +/- strand): ref base . (11/6); new base A (439/444); total (453/450)
Fisher's exact test for biased strand distribution p-value = 3.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 956 total aligned reads displayed.

TTCAGTCAAAATTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAG‑AA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGCATCAA  >  AM406671/1210130‑1210421

       aaaaTTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAG‑aa‑aaaat                                                                                                                                          >  2:579607/1‑147 (MQ=255)

         aaTTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAG‑aa‑aaaatat                                                                                                                                        >  1:2532353/1‑145 (MQ=255)

         aaTTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAG‑aa‑aaaatat                                                                                                                                        <  1:9329897/149‑5 (MQ=255)

          aTTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAG‑aa‑aaaatatt                                                                                                                                       >  1:7209526/1‑144 (MQ=255)

           tttCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATAttt                                                                                                                                       >  1:4653663/1‑149 (MQ=255)

            ttCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTg                                                                                                                                      <  2:4965218/149‑1 (MQ=255)

             tctcGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGc                                                                                                                                     <  2:4439333/149‑1 (MQ=255)

               tcGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAG‑aa‑aaaa                                                                                                                                           >  1:8988898/1‑139 (MQ=255)

                cGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGa                                                                                                                                  <  1:837708/149‑1 (MQ=255)

                cGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGa                                                                                                                                  <  1:2785360/149‑1 (MQ=255)

                cGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGa                                                                                                                                  <  1:10971623/149‑1 (MQ=255)

                cGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCATTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGa                                                                                                                                  >  2:6837839/1‑149 (MQ=255)

                 gAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGAc                                                                                                                                 <  2:8397449/149‑1 (MQ=255)

                   aaaCTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAg                                                                                                                               >  1:4477324/1‑149 (MQ=255)

                       tATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTATAAAAAGAGAAA‑AAATATTTGCTGACAGATGa                                                                                                                           <  2:799932/149‑1 (MQ=255)

                        aTCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAg                                                                                                                          >  1:1401342/1‑149 (MQ=255)

                        aTCTGTTCTCTCCTGAGTCATCAATGTATTGGACGAACCAACAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTAAAAAAAAAGAAA‑AAATATTTGCTGACAGATGAg                                                                                                                          <  2:4246731/149‑1 (MQ=255)

                         tCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGt                                                                                                                         >  2:1835825/1‑149 (MQ=255)

                                   ccTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGt                                                                                                               <  1:10389968/149‑1 (MQ=255)

                                       aGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCaa                                                                                                           >  1:2656618/1‑149 (MQ=255)

                                        gTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAg                                                                                                          <  2:11114027/149‑1 (MQ=255)

                                          cATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTa                                                                                                        <  1:3162040/149‑1 (MQ=255)

                                             ccATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGaa                                                                                                     <  2:692408/149‑1 (MQ=255)

                                                 gTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAg                                                                                                 >  2:5967901/1‑149 (MQ=255)

                                                  tATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAgg                                                                                                <  1:2332825/149‑1 (MQ=255)

                                                   aTTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAggg                                                                                               <  1:7439782/149‑1 (MQ=255)

                                                      ggACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGaa                                                                                            >  2:6379039/1‑149 (MQ=255)

                                                        aCGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAAtt                                                                                          >  1:9114298/1‑149 (MQ=255)

                                                          gAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTc                                                                                        >  2:1634127/1‑149 (MQ=255)

                                                          gAACCACCAATGAAAGCGAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTc                                                                                        <  1:11322165/149‑1 (MQ=255)

                                                           aaCCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCt                                                                                       <  1:4304841/149‑1 (MQ=255)

                                                            accaccAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCtt                                                                                      >  1:3409565/1‑149 (MQ=255)

                                                              caccaATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTg                                                                                    >  1:8744007/1‑149 (MQ=255)

                                                               accaATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTgg                                                                                   >  1:10496442/1‑149 (MQ=255)

                                                                ccaATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGc                                                                                  <  2:7302263/149‑1 (MQ=255)

                                                                     gAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGTGGAATTTCTTTGGCTAAAt                                                                             >  1:5605478/1‑149 (MQ=255)

                                                                       aaGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTg                                                                           <  1:6883880/149‑1 (MQ=255)

                                                                       aaGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTg                                                                           <  2:884271/149‑1 (MQ=255)

                                                                        aGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGCATAAGGGGAATTTCTTTGGCTAAATTGc                                                                          >  2:2693260/1‑149 (MQ=255)

                                                                        aGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGc                                                                          <  2:2367978/149‑1 (MQ=255)

                                                                        aGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGc                                                                          >  1:9809098/1‑149 (MQ=255)

                                                                        aGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGc                                                                          >  1:2899338/1‑149 (MQ=255)

                                                                          cTAATTTCTGATTGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTa                                                                        >  2:10471229/1‑149 (MQ=255)

                                                                          cTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTa                                                                        <  2:10919659/149‑1 (MQ=255)

                                                                           tAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTaa                                                                       <  1:3303857/149‑1 (MQ=255)

                                                                            aaTTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAag                                                                      <  2:3082638/149‑1 (MQ=255)

                                                                            aaTTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAag                                                                      >  2:3950736/1‑149 (MQ=255)

                                                                             aTTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAggg                                                                                               <  2:5088264/123‑1 (MQ=255)

                                                                             aTTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAaga                                                                     <  1:8621807/149‑1 (MQ=255)

                                                                              tttCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAagag                                                                    >  1:3604759/1‑149 (MQ=255)

                                                                              tttCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAagag                                                                    <  1:4814659/149‑1 (MQ=255)

                                                                                  tGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGagcagc                                                                <  1:5417846/149‑1 (MQ=255)

                                                                                  tGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGagcagc                                                                >  2:7111633/1‑149 (MQ=255)

                                                                                     tAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTg                                                             >  1:10020691/1‑149 (MQ=255)

                                                                                     tAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTg                                                             >  1:7955194/1‑149 (MQ=255)

                                                                                      aGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTTGTCTCTTTTTAGAGAAA‑AAATATTTGCTTACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTgg                                                            <  2:6746101/149‑1 (MQ=255)

                                                                                          tttttGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGAAGCTTGGTAGt                                                        >  2:9654703/1‑149 (MQ=255)

                                                                                           ttttGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTc                                                       <  2:3302405/149‑1 (MQ=255)

                                                                                            tttGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGTATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCa                                                      >  1:8192991/1‑149 (MQ=255)

                                                                                                 aTCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGAtt                                                 >  1:5831515/1‑149 (MQ=255)

                                                                                                  tCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTa                                                <  2:9845071/149‑1 (MQ=255)

                                                                                                    aTGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAat                                              <  2:3509144/149‑1 (MQ=255)

                                                                                                     tgtgAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAata                                             >  1:6219262/1‑149 (MQ=255)

                                                                                                     tgtgAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAata                                             >  2:10688891/1‑149 (MQ=255)

                                                                                                      tggcgaAACAAGTAGTTGAGTGGAAGTACGTTGATCTCTTTTTAAAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAatat                                            <  2:10316386/145‑1 (MQ=255)

                                                                                                       tgAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTa                                                                                                        >  2:2171401/1‑88 (MQ=255)

                                                                                                       tgAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTa                                                                                                        <  1:2171401/88‑1 (MQ=255)

                                                                                                         agaAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAg                                         <  2:4854086/149‑1 (MQ=255)

                                                                                                         agaAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAg                                         <  2:1158518/149‑1 (MQ=255)

                                                                                                            aaCAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAG‑AATTAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGttt                                      >  2:9129758/1‑149 (MQ=255)

                                                                                                                 gtagtTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCt                                 >  1:7673440/1‑149 (MQ=255)

                                                                                                                   agtTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCtaa                               <  2:5422623/149‑1 (MQ=255)

                                                                                                                   agtTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCtaa                               >  1:1564647/1‑149 (MQ=255)

                                                                                                                    gtTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCtaat                              <  2:3820986/149‑1 (MQ=255)

                                                                                                                    gtTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCtaat                              <  1:8915365/149‑1 (MQ=255)

                                                                                                                    gtTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCtaat                              <  1:4923362/149‑1 (MQ=255)

                                                                                                                       gAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTaataat                           >  2:10038763/1‑149 (MQ=255)

                                                                                                                        aGTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAAtc                          <  1:6672928/149‑1 (MQ=255)

                                                                                                                         gTGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAAtcc                         <  1:4116584/149‑1 (MQ=255)

                                                                                                                          tGGGAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAAtcct                        >  1:5088418/1‑149 (MQ=255)

                                                                                                                           gggAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAAtcctc                       <  1:5967566/149‑1 (MQ=255)

                                                                                                                           gggAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAAtcctc                       >  2:6150498/1‑149 (MQ=255)

                                                                                                                            ggAGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAAtcctcc                      <  2:2532353/149‑1 (MQ=255)

                                                                                                                              aGTGCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTa                    <  2:9485819/149‑1 (MQ=255)

                                                                                                                                 gCGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATTAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTaaaa                 >  2:2904572/1‑149 (MQ=255)

                                                                                                                                  cGTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAAt                <  2:7562385/149‑1 (MQ=255)

                                                                                                                                   gTTGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAAtt               <  2:5546096/149‑1 (MQ=255)

                                                                                                                                     tGGTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTaa             <  2:8673083/149‑1 (MQ=255)

                                                                                                                                       gactctTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTCAAACACGTCCAAGTAGAATGCGGGGAATTTCTCTGGCTAAATTGCTAAGAGCAGTTTGGTAGGCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTaaaa           <  2:8229935/147‑1 (MQ=255)

                                                                                                                                       gTCTCTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTaaaa           >  1:9664788/1‑149 (MQ=255)

                                                                                                                                        tctcTTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAAc          >  1:1226504/1‑149 (MQ=255)

                                                                                                                                         ctctTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACt         >  2:1324764/1‑149 (MQ=255)

                                                                                                                                         ctctTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCACCTAATAATCCTCCTAAAATTAAAACt         >  1:6442383/1‑149 (MQ=255)

                                                                                                                                          tctTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTg        >  1:1847573/1‑149 (MQ=255)

                                                                                                                                          tctTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTg        <  1:10740614/149‑1 (MQ=255)

                                                                                                                                           ctTTTTAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGc       <  2:9284224/149‑1 (MQ=255)

                                                                                                                                             ttttAGAGAAA‑AAATATTTGCTGACAGAGGAGCTACAACTTGTCTAAGTATTATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATATAATTATCCTCCTAAAATTAAAACTGCAt     >  2:4655775/1‑149 (MQ=255)

                                                                                                                                              tttAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGCATc    >  1:6753863/1‑149 (MQ=255)

                                                                                                                                              tttAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGCATc    >  1:3938318/1‑149 (MQ=255)

                                                                                                                                               ttAGAGAAA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGCATCa   <  1:10237903/149‑1 (MQ=255)

TTCAGTCAAAATTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAG‑AA‑AAATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGCATCAA  >  AM406671/1210130‑1210421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`