Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 912,618 T→A L46Q (CTA→CAA)  llmg_0944 → putative secreted protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM406671912,6180TA99.7% 4082.8 / NA 1044L46Q (CTA→CAA) llmg_0944putative secreted protein
Reads supporting (aligned to +/- strand):  ref base T (3/0);  new base A (570/470);  total (574/470)
Fisher's exact test for biased strand distribution p-value = 2.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1046 total aligned reads displayed.

AGGAAAATAAATGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCTAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAATTTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTA  >  AM406671/912472‑912763
                                                                                                                                                  |                                                                                                                                                 
    aaataaatGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGa                                                                                                                                             <  1:6557994/149‑1 (MQ=255)
      ataaatGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGata                                                                                                                                           <  1:9625234/149‑1 (MQ=255)
      ataaatGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGata                                                                                                                                           >  2:5925390/1‑149 (MQ=255)
       taaatGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGataa                                                                                                                                          >  1:1002968/1‑149 (MQ=255)
       taaatGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGataa                                                                                                                                          >  1:10563695/1‑149 (MQ=255)
        aaatGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGataat                                                                                                                                         <  2:3213096/149‑1 (MQ=255)
         aatGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGataata                                                                                                                                        >  2:10592967/1‑149 (MQ=255)
          atGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAatat                                                                                                                                       >  1:5159781/1‑149 (MQ=255)
            gAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTg                                                                                                                                     >  1:1212679/1‑149 (MQ=255)
              atacTAGAAAAAGGAAAACAGAGAAGAAACGCAGAGGGGGGAAAGTATTTGCAATGTTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTgca                                                                                                                                   <  2:5760206/146‑1 (MQ=255)
                 cTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTgcagca                                                                                                                                >  1:9437852/1‑149 (MQ=255)
                    gaaaaagaaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCaaaa                                                                                                                             >  1:1751333/1‑149 (MQ=255)
                      aaaagaaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAAtt                                                                                                                           >  2:2607230/1‑149 (MQ=255)
                       aaagaaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAAttt                                                                                                                          <  2:6057227/149‑1 (MQ=255)
                         agaaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTtat                                                                                                                        >  2:8956034/1‑149 (MQ=255)
                           aaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATAc                                                                                                                      <  1:3155211/149‑1 (MQ=255)
                             aaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTCGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCa                                                                                                                    <  1:7140931/149‑1 (MQ=255)
                              aaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAg                                                                                                                   <  2:2995502/149‑1 (MQ=255)
                              aaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAg                                                                                                                   >  1:10810319/1‑149 (MQ=255)
                              aaCAGAGAAGAAACGCAGACGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGTTCTGATTGTTTTTGGTATTGTAATGATAGGACGACTCGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTTTACCAg                                                                                                                   <  2:5547289/149‑1 (MQ=255)
                               aCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGa                                                                                                                  >  2:1238101/1‑149 (MQ=255)
                                  gagaAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAAtc                                                                                                               <  1:9923838/149‑1 (MQ=255)
                                   agaagaAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAAtct                                                                                                              <  1:924514/149‑1 (MQ=255)
                                     aagaaACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCa                                                                                                            >  2:1665228/1‑149 (MQ=255)
                                      agaaACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCaa                                                                                                           >  2:8445488/1‑149 (MQ=255)
                                        aaaCGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCaaaa                                                                                                         <  1:2539949/149‑1 (MQ=255)
                                        aaaCGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCaaaa                                                                                                         >  2:9132243/1‑149 (MQ=255)
                                          aCGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACt                                                                                                       >  2:2831766/1‑149 (MQ=255)
                                                agGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACgg                                                                                                 >  1:8865267/1‑149 (MQ=255)
                                                 gggggTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTCCAGCAAAATTTATACCAGAATCTCAAACCTTAACGGa                                                                                                >  2:8680584/1‑149 (MQ=255)
                                                  ggggTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGAt                                                                                               <  1:368736/149‑1 (MQ=255)
                                                   gggTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATg                                                                                              <  2:4197723/149‑1 (MQ=255)
                                                      tAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAg                                                                                           <  2:227419/149‑1 (MQ=255)
                                                      tAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAg                                                                                           >  1:3886060/1‑149 (MQ=255)
                                                      tAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAg                                                                                           >  1:5811858/1‑149 (MQ=255)
                                                       aaaGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGc                                                                                          >  2:3883541/1‑149 (MQ=255)
                                                         aGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCgg                                                                                        <  1:3298341/149‑1 (MQ=255)
                                                         aGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCgg                                                                                        >  1:4460651/1‑149 (MQ=255)
                                                         aGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCgg                                                                                        >  2:2441726/1‑149 (MQ=255)
                                                           tATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGAt                                                                                      <  2:6567168/149‑1 (MQ=255)
                                                           tATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGAt                                                                                      >  2:7375251/1‑149 (MQ=255)
                                                              ttGCAATATTACTCTCCCTTATTATCTTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCaa                                                                                   >  2:6225056/1‑149 (MQ=255)
                                                               tGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCaaa                                                                                  >  1:6949880/1‑149 (MQ=255)
                                                                 cAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAAtt                                                                                <  2:9776139/149‑1 (MQ=255)
                                                                 cAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAAtt                                                                                >  2:8088092/1‑149 (MQ=255)
                                                                 cAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGAACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAAtt                                                                                >  1:6732364/1‑149 (MQ=255)
                                                                   atatTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTgg                                                                              >  1:2357560/1‑149 (MQ=255)
                                                                      ttACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTc                                                                           >  1:1411138/1‑149 (MQ=255)
                                                                      ttACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTCATTCCTAATCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTc                                                                           >  2:7163663/1‑149 (MQ=255)
                                                                         ctctCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGTCGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACTAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGc                                                                        >  2:7681378/1‑149 (MQ=255)
                                                                         ctctCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGc                                                                        >  1:4765827/1‑149 (MQ=255)
                                                                          tctcCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCt                                                                       <  1:9809755/149‑1 (MQ=255)
                                                                              ccTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTc                                                                   >  1:2857506/1‑149 (MQ=255)
                                                                              ccTTATTATATTTCTGGCGCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTc                                                                   <  1:1108911/149‑1 (MQ=255)
                                                                               cTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTcc                                                                  >  2:10068083/1‑149 (MQ=255)
                                                                                ttattaTATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCg                                                                 >  2:10265583/1‑149 (MQ=255)
                                                                                ttattaTATTTCTGGCTCTGATTGTTTTTGGTATTGTAAGGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATATCAAAACTGAACGGATGCAGCGGATCAAATTGGTTCGGCTACTACg                                                                 >  2:6386045/1‑149 (MQ=255)
                                                                                 tattatATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGt                                                                >  2:5332418/1‑149 (MQ=255)
                                                                                 tattatATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGt                                                                >  1:10385704/1‑149 (MQ=255)
                                                                                   ttatACTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCa                                                              <  2:4330801/149‑1 (MQ=255)
                                                                                     atatTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGc                                                            <  1:4344746/149‑1 (MQ=255)
                                                                                     atatTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGc                                                            >  1:7975661/1‑149 (MQ=255)
                                                                                      tatTTCTGGCTCTGATTGTTTTTGGTATAGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGAAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAAATGGTTCGGCTACTCCGTCAGCt                                                           <  2:8574812/149‑1 (MQ=255)
                                                                                      tatTGCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCt                                                           <  2:4626908/149‑1 (MQ=255)
                                                                                       atTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTa                                                          <  1:8212351/149‑1 (MQ=255)
                                                                                         ttCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTAtt                                                        <  2:9513874/149‑1 (MQ=255)
                                                                                          tCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTg                                                       >  1:2105987/1‑149 (MQ=255)
                                                                                           cTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAAAGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTgc                                                      <  2:8247969/149‑1 (MQ=255)
                                                                                            tGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTgca                                                     <  2:5215134/149‑1 (MQ=255)
                                                                                             ggCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTgcaa                                                    >  2:6892397/1‑149 (MQ=255)
                                                                                                 ctGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTgcaagcaa                                                <  2:9196170/149‑1 (MQ=255)
                                                                                                  tGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTgcaagcaaa                                               <  2:9350299/149‑2 (MQ=255)
                                                                                                   gATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTgcaagcaaat                                              >  2:4487663/1‑147 (MQ=255)
                                                                                                   gATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTgcaagcaaat                                              >  2:10375975/1‑147 (MQ=255)
                                                                                                   gATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTgcaagcaaat                                              <  1:6462631/149‑3 (MQ=255)
                                                                                                    aTTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTgcaagcaaatt                                             >  1:7386651/1‑146 (MQ=255)
                                                                                                      tGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCTGCTATTGCAAGCAAATTaa                                           >  1:5640187/1‑149 (MQ=255)
                                                                                                         ttttGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATg                                        >  1:5341670/1‑149 (MQ=255)
                                                                                                         ttttGGTATTGTAATAATAGGACGACACGTTATTTCTAGTCAAACATATAATATTACTGCCTAATTTATACCAGAATCTAAAAACTTACCGGATACAGCGGATCAAATTTGTACGGCTAATCCGTCAACTATTACAAGCACATTAAATg                                        >  2:3700640/1‑149 (MQ=255)
                                                                                                          tttGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATga                                       <  1:4128488/149‑1 (MQ=255)
                                                                                                            tGGTATTGTAATGGTAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATgaga                                     <  1:3458758/149‑1 (MQ=255)
                                                                                                              gTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACTGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAAt                                   <  1:837919/149‑1 (MQ=255)
                                                                                                               tATTGTAATGATAGGACAACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATAACAGAATCTCAAACCTTAACGGATGCAGCGGATCAAATTGCTTCGGCTACTCCGTCATCTATTGCAAGCAAATTAGATGAGAAtt                                  <  2:11142755/149‑1 (MQ=255)
                                                                                                                    tAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGAt                             <  2:7895948/149‑1 (MQ=255)
                                                                                                                      aTGATAGTACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATAc                           <  2:1470284/149‑1 (MQ=255)
                                                                                                                      aTGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATgaga                                     <  1:5009903/139‑1 (MQ=255)
                                                                                                                      aTGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATAc                           >  2:4800248/1‑149 (MQ=255)
                                                                                                                      aTGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATAc                           >  1:9060981/1‑149 (MQ=255)
                                                                                                                       tGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATAcc                          <  1:1637127/149‑1 (MQ=255)
                                                                                                                        gATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCa                         <  1:7699920/149‑1 (MQ=255)
                                                                                                                         aTAGGACGACACGTTATCTCTAGTCAAACAGATAATATGGCAGCAAAATTTATACCAGAATCTCAAAACTTAGCGGACGCAGCGGATCAAATTGGTTCGGCTTCTCCGTCCGCTATTGCAAGCAAACTAACTGCGAATTCGGATAcctt                        <  2:5017950/149‑3 (MQ=255)
                                                                                                                             gacgacACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAag                    >  1:6046851/1‑149 (MQ=255)
                                                                                                                              acgacACGTTATTTCTAGTCAAACAGATAGTATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAaga                   <  2:6781714/149‑1 (MQ=255)
                                                                                                                                  cgcGTTATTTCTAGTCAAACAGATAATATTGAAGCAAAATGTATACCGGACCCTCAAAGCTTAACGGATGCAGCGGATCAAAGTGGTTCGGCTACTCCGGCAGCTATTGCAAGCAACTTAACTGAGAAGGCGGATACCGCCAAGAGTCa               <  2:3481668/147‑1 (MQ=255)
                                                                                                                                        aTTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATTTCa         >  2:1917522/1‑149 (MQ=255)
                                                                                                                                        aTTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCa         >  2:10909195/1‑149 (MQ=255)
                                                                                                                                         tttCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAg        <  2:11369599/149‑1 (MQ=255)
                                                                                                                                           tCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAggg      >  1:619476/1‑149 (MQ=255)
                                                                                                                                             tAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGAt    >  1:8536996/1‑149 (MQ=255)
                                                                                                                                              aGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGAtt   >  1:10961760/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AGGAAAATAAATGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCTAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAATTTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTA  >  AM406671/912472‑912763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑