Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 295,026 T→C S40P (TCG→CCG)  rimI2 → Ribosomal‑protein‑alanine acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM406671295,0260TC99.8% 6133.8 / NA 1510S40P (TCG→CCG) rimI2Ribosomal‑protein‑alanine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  major base C (811/692);  minor base A (2/1);  total (815/695)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1514 total aligned reads displayed.

GCAATTTGGATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGTCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCAATATCGCCATTC  >  AM406671/294880‑295170
                                                                                                                                                  |                                                                                                                                                
gCAATTTGGATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCg                                                                                                                                                >  1:6992723/1‑149 (MQ=255)
gCAATTTGGATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAAAAAGTCGAAAGACAAAAATACTTTTCCACATGTGGAAAAGTTATAAGTCAATATTTATGAACTATTATCAGTGGCTTATGGAAAGACg                                                                                                                                                >  2:5713106/1‑149 (MQ=255)
         aTGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTTTCTTACTCTGAAAATAAAACGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTca                                                                                                                                       <  2:7733044/149‑1 (MQ=255)
         aTGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTca                                                                                                                                       <  2:3315020/149‑1 (MQ=255)
          tGGTCAAAGAATTAAAAAAATGAATTTAAACCGTCTGTCGTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTcac                                                                                                                                      <  1:3429487/149‑1 (MQ=255)
           ggTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTcaca                                                                                                                                     <  1:2025575/149‑1 (MQ=255)
                aaGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGttt                                                                                                                                >  1:11196080/1‑149 (MQ=255)
                 aGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTta                                                                                                                               >  1:6214166/1‑149 (MQ=255)
                  gAATTAAAAAAATGAAGTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTtat                                                                                                                              <  2:3731757/149‑1 (MQ=255)
                    aTTAAAAAAACGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTtatat                                                                                                                            <  1:10580712/149‑1 (MQ=255)
                        aaaaaaTGAATTTAAAACGCCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCg                                                                                                                        >  1:11337493/1‑149 (MQ=255)
                         aaaaaTGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGa                                                                                                                       >  2:10758482/1‑149 (MQ=255)
                            aaTGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTg                                                                                                                    <  2:10485677/149‑1 (MQ=255)
                             aTGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGa                                                                                                                   >  1:8822216/1‑149 (MQ=255)
                               gAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGAtt                                                                                                                 <  1:786657/149‑1 (MQ=255)
                                aaTTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGAttt                                                                                                                >  1:919550/1‑149 (MQ=255)
                                 aTTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTa                                                                                                               >  2:3136918/1‑149 (MQ=255)
                                 aTTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTa                                                                                                               >  1:4994268/1‑149 (MQ=255)
                                  tttAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAg                                                                                                              >  2:4479226/1‑149 (MQ=255)
                                   ttAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGt                                                                                                             <  2:1601796/149‑1 (MQ=255)
                                    tAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTa                                                                                                            >  1:1369595/1‑149 (MQ=255)
                                        aCGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACg                                                                                                        <  2:6319065/149‑1 (MQ=255)
                                         cGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGa                                                                                                       <  1:10971637/149‑1 (MQ=255)
                                          gtctgtctTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGaa                                                                                                      <  1:1222963/149‑1 (MQ=255)
                                           tctgtctTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACGAGTTTATATATCGAGTGATTTAGTAAACGaaa                                                                                                     <  2:4870917/149‑1 (MQ=255)
                                            ctgtctTACTCTGAAAATAAGTCGAAAGAGAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGaaaa                                                                                                    >  2:7027062/1‑149 (MQ=255)
                                             tgtctTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAAt                                                                                                   >  1:5932418/1‑149 (MQ=255)
                                                 ttACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCgg                                                                                               >  1:2281706/1‑149 (MQ=255)
                                                  tACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGa                                                                                              >  2:9820052/1‑149 (MQ=255)
                                                   aCTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGaa                                                                                             <  2:10072163/149‑1 (MQ=255)
                                                    ctctGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAAt                                                                                            >  1:2710403/1‑149 (MQ=255)
                                                      ctGAAAATAAGTCGAAAGATAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATAc                                                                                          <  1:5564739/149‑1 (MQ=255)
                                                      ctGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATAc                                                                                          <  1:938424/149‑1 (MQ=255)
                                                       tGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACt                                                                                         <  1:6444784/149‑1 (MQ=255)
                                                       tGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACt                                                                                         >  2:9618079/1‑149 (MQ=255)
                                                       tGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACt                                                                                         >  1:5388440/1‑149 (MQ=255)
                                                        gAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACtt                                                                                        >  1:4422253/1‑149 (MQ=255)
                                                        gAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACtt                                                                                        >  1:250909/1‑149 (MQ=255)
                                                         aaaaTAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACttt                                                                                       >  1:9890520/1‑149 (MQ=255)
                                                          aaaTAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGCACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACttgt                                                                                      >  2:4224545/1‑147 (MQ=255)
                                                           aaTAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACttttt                                                                                     <  1:9464562/149‑1 (MQ=255)
                                                            aTAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTa                                                                                    >  1:10006631/1‑149 (MQ=255)
                                                            aTAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGATTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGGGTGAGTTAGTAAACGGAAATTGGGAGTACTTCTTa                                                                                    >  2:4705047/1‑149 (MQ=255)
                                                             tAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTAc                                                                                   <  1:8394918/149‑1 (MQ=255)
                                                             tAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTAc                                                                                   >  1:4076243/1‑149 (MQ=255)
                                                             tAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTGAGTAAACGAAAATTCGGAATACTTTTTAc                                                                                   >  2:9151490/1‑149 (MQ=255)
                                                              aaGTCGAAAGACAAAAATACTTTTCCACATGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACt                                                                                  <  1:8239213/149‑1 (MQ=255)
                                                              aaGACGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTCTATATATCGAGTGATTTAGTCAAGGAAAATGCGGAATGCTGTTGACt                                                                                  >  2:3560185/1‑149 (MQ=255)
                                                                gTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTAGGAATACTTTTTATTag                                                                                >  2:20465/1‑149 (MQ=255)
                                                                 tCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTaga                                                                               >  1:2965839/1‑149 (MQ=255)
                                                                 tCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTaga                                                                               >  1:8048288/1‑149 (MQ=255)
                                                                 tCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTACGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTaga                                                                               >  1:7214302/1‑149 (MQ=255)
                                                                    aaaGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTagaaga                                                                            >  1:1721908/1‑149 (MQ=255)
                                                                     aataCAAAAATACTTTTCCACCTGTGGAAAAGGTATCAGTCAATATTCATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTGTATATATCGAGTGATTTAGTAACCGAAAAATCTGAATACTTTTTACTagaagaa                                                                           <  1:10786891/146‑1 (MQ=255)
                                                                     aaGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTagaagaa                                                                           >  1:3176774/1‑149 (MQ=255)
                                                                       gACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTagaagaaga                                                                         >  2:9318347/1‑149 (MQ=255)
                                                                         cAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATg                                                                       <  1:4570964/149‑1 (MQ=255)
                                                                         cAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATg                                                                       <  1:5175796/149‑1 (MQ=255)
                                                                           aaaaTACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAg                                                                     >  1:150521/1‑149 (MQ=255)
                                                                            aaaTACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGc                                                                    >  2:2682404/1‑149 (MQ=255)
                                                                            aaaTACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGc                                                                    >  1:9697393/1‑149 (MQ=255)
                                                                            aaaTACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGc                                                                    <  1:10211606/149‑1 (MQ=255)
                                                                            aaaTACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGc                                                                    <  1:8959518/149‑1 (MQ=255)
                                                                             aaTACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCa                                                                   <  2:10262445/149‑1 (MQ=255)
                                                                                aCTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCaatt                                                                <  2:1371876/149‑1 (MQ=255)
                                                                                 cTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCaatta                                                               >  2:6541692/1‑149 (MQ=255)
                                                                                   tttCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCaattaat                                                             <  1:6767091/149‑1 (MQ=255)
                                                                                      ccACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTgg                                                          <  2:3029050/149‑1 (MQ=255)
                                                                                       cACCTGTGAAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATAGCGGGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGt                                                         <  2:2206321/149‑1 (MQ=255)
                                                                                        aCCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTc                                                        >  1:10441193/1‑149 (MQ=255)
                                                                                          cTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTctg                                                      >  2:2400396/1‑149 (MQ=255)
                                                                                            gtgGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTctgct                                                    >  2:8635416/1‑149 (MQ=255)
                                                                                            gtgGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTctgct                                                    >  1:4817371/1‑149 (MQ=255)
                                                                                             tgGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTctgctg                                                   <  2:8848405/149‑1 (MQ=255)
                                                                                             tgGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTctgctg                                                   >  2:6172707/1‑149 (MQ=255)
                                                                                              ggAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTgg                                                  <  2:5054721/149‑1 (MQ=255)
                                                                                               gAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTATGCTGGc                                                 >  1:8632112/1‑149 (MQ=255)
                                                                                                 aaaGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACGTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCa                                               <  1:3778101/149‑1 (MQ=255)
                                                                                                  aaGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCAt                                              >  1:7378590/1‑149 (MQ=255)
                                                                                                   aGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCAtt                                             <  2:7971407/149‑1 (MQ=255)
                                                                                                      tATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTAACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCa                                          <  1:575612/149‑1 (MQ=255)
                                                                                                         cAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCaata                                       >  2:1127918/1‑149 (MQ=255)
                                                                                                         cAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCaata                                       <  2:10626486/149‑1 (MQ=255)
                                                                                                         aaGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCaata                                       <  2:8161814/148‑1 (MQ=255)
                                                                                                           gTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCaataat                                     >  2:6008077/1‑149 (MQ=255)
                                                                                                               atatTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAatgatg                                 >  1:3610401/1‑149 (MQ=255)
                                                                                                                  tttATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATgatggat                              <  1:4231234/149‑1 (MQ=255)
                                                                                                                    tATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGa                            <  2:8441772/149‑1 (MQ=255)
                                                                                                                      tGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAAt                          >  2:6691255/1‑149 (MQ=255)
                                                                                                                      tGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAAt                          >  2:2801974/1‑149 (MQ=255)
                                                                                                                      tGAACTTTTATCAGTGGCTTATCGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAAt                          <  2:7290967/149‑1 (MQ=255)
                                                                                                                         aCTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAg                       <  2:548649/149‑1 (MQ=255)
                                                                                                                         aCTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAg                       <  1:3865607/149‑1 (MQ=255)
                                                                                                                            tttATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAg                    <  2:11034944/149‑1 (MQ=255)
                                                                                                                            tttATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGCCCATTTCACTAATGATGGGAGAACTAGAAg                    >  2:7553423/1‑149 (MQ=255)
                                                                                                                             ttATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGt                   <  2:8976115/149‑1 (MQ=255)
                                                                                                                                        ttATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCAATATCg        <  2:4023250/149‑1 (MQ=255)
                                                                                                                                          aTGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCAATATCGcc      >  1:7557170/1‑149 (MQ=255)
                                                                                                                                           tGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCAATATCGCCa     <  2:1187204/149‑1 (MQ=255)
                                                                                                                                            ggACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCAATATCGCCAt    <  2:5259082/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GCAATTTGGATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGTCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCAATATCGCCATTC  >  AM406671/294880‑295170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑