Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 1,081,552 T→A V212D (GTT→GAT)  ldh → L‑lactate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM4066711,081,5520TA99.9% 4034.0 / ‑5.2 1044V212D (GTT→GAT) ldhL‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base A (536/506);  minor base G (1/0);  total (538/506)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

Only 100 of 1044 total aligned reads displayed.

TGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTCCTGTATCAGTATTC  >  AM406671/1081406‑1081697
                                                                                                                                                  |                                                                                                                                                 
   cGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAAATTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAAt                                                                                                                                              >  2:7823435/1‑149 (MQ=255)
           cTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAg                                                                                                                                      >  1:5244229/1‑149 (MQ=255)
               ttCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTg                                                                                                                                  <  2:606032/149‑1 (MQ=255)
                 cAATCCACGCATACATCATGGGTGAACACCGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTa                                                                                                                                <  2:6687062/149‑1 (MQ=255)
                  aaTCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTAc                                                                                                                               >  1:7720428/1‑149 (MQ=255)
                     ccACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTg                                                                                                                            <  1:504678/149‑1 (MQ=255)
                      cACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGa                                                                                                                           >  1:4489900/1‑149 (MQ=255)
                       aCGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGAt                                                                                                                          >  1:2102330/1‑149 (MQ=255)
                       aCGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTCGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAACTGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGAt                                                                                                                          >  2:11086766/1‑149 (MQ=255)
                        cGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATg                                                                                                                         <  1:9503034/149‑1 (MQ=255)
                          cATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGCACAATGGTTCCAAGAAAATGACTACCTTAAAGAAGCAGAAAGAGATGAATTCTTTGAGTCAGTAAGTGCTGCa                                                                                                                       >  2:7657344/1‑149 (MQ=255)
                          cATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCa                                                                                                                       <  1:1672054/149‑1 (MQ=255)
                          cATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCa                                                                                                                       >  2:10135869/1‑149 (MQ=255)
                           aTACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAg                                                                                                                      <  2:2796681/149‑1 (MQ=255)
                            tACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGc                                                                                                                     >  1:10335932/1‑149 (MQ=255)
                            tACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGc                                                                                                                     >  1:7173377/1‑149 (MQ=255)
                             aCATCATGGGTGAACACGGTTACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATTGTTCCAAAAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCt                                                                                                                    <  2:5871639/149‑1 (MQ=255)
                             aCATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCt                                                                                                                    >  2:10248338/1‑149 (MQ=255)
                             aCATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCt                                                                                                                    >  1:2685879/1‑149 (MQ=255)
                              catcatGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCtt                                                                                                                   <  1:4121279/149‑1 (MQ=255)
                                     ggTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAat                                                                                                            <  2:5715724/149‑1 (MQ=255)
                                     ggTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAat                                                                                                            >  1:10648575/1‑149 (MQ=255)
                                        gAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAatcat                                                                                                         <  1:11116826/149‑1 (MQ=255)
                                        gAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAatcat                                                                                                         >  1:8614974/1‑149 (MQ=255)
                                          acacGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCg                                                                                                       >  1:3590383/1‑149 (MQ=255)
                                               gTGACTCAGAATTTGCTGTTTGGTCATACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACNTGATGCAGCTTACTCAATCATCGCTaaa                                                                                                  <  1:6361596/149‑1 (MQ=255)
                                                tGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGtt                                                                                                                                          <  1:10477854/108‑1 (MQ=255)
                                                  aCTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTaaaaaa                                                                                               >  2:6332577/1‑149 (MQ=255)
                                                     cAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGt                                                                                            >  1:4299889/1‑149 (MQ=255)
                                                        aaTTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCa                                                                                         <  2:10817605/149‑1 (MQ=255)
                                                         aTTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCaa                                                                                        <  1:11321659/149‑1 (MQ=255)
                                                          tttGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAAc                                                                                       >  1:2513770/1‑149 (MQ=255)
                                                           ttGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACa                                                                                      <  1:5749962/149‑1 (MQ=255)
                                                            tGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACAt                                                                                     <  2:8262224/149‑1 (MQ=255)
                                                             gCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACAtt                                                                                    >  1:133902/1‑149 (MQ=255)
                                                             gCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACAtt                                                                                    <  2:2983593/149‑1 (MQ=255)
                                                             gCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACAtt                                                                                    <  2:8465877/149‑1 (MQ=255)
                                                             gCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTCCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATAATCGATAAAAAAGGTGCAACAtt                                                                                    <  2:9355197/149‑1 (MQ=255)
                                                              cTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTc                                                                                   <  1:10002548/149‑1 (MQ=255)
                                                                gTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTa                                                                                 >  2:11312888/1‑149 (MQ=255)
                                                                 tttGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTAc                                                                                >  1:9247053/1‑149 (MQ=255)
                                                                   tGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACgg                                                                              <  1:7901653/149‑1 (MQ=255)
                                                                      tCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGgtgt                                                                           <  2:5525834/149‑1 (MQ=255)
                                                                        acacGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTgg                                                                         <  1:8772538/149‑1 (MQ=255)
                                                                         cacGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGc                                                                        <  2:9513934/149‑1 (MQ=255)
                                                                           cGCTAACGGGGCTGGTGTTAAAATGGAACAATGGTGCAAAGAAAATGACTACCTTAACGAAGCAGAAAGCGATGAATTGTTAAAGTCTGTACGTGATAAAGCTTACTAAATCAACGATAAAAAAGGTGCAACATTCTACGGTGTGGCTg                                                                      <  2:4676832/149‑1 (MQ=255)
                                                                                 cGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGccc                                                                >  2:2023354/1‑149 (MQ=255)
                                                                                  gTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCt                                                               >  1:303746/1‑149 (MQ=255)
                                                                                   ttGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTCAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCtt                                                              >  2:3467819/1‑149 (MQ=255)
                                                                                    tGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTg                                                             <  1:8936816/149‑1 (MQ=255)
                                                                                    tGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTg                                                             <  1:3292996/149‑1 (MQ=255)
                                                                                    tGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTg                                                             <  1:3402482/149‑1 (MQ=255)
                                                                                     gCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGc                                                            >  2:5149311/1‑149 (MQ=255)
                                                                                     gCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGc                                                            >  1:4851008/1‑149 (MQ=255)
                                                                                      cTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCt                                                           <  2:8868747/149‑1 (MQ=255)
                                                                                       tGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGGGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGTTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTc                                                          <  2:2654143/149‑1 (MQ=255)
                                                                                         gtgtTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCTATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGGGGCTGTAGCCCTTGCTCGt                                                        <  2:2422206/149‑1 (MQ=255)
                                                                                         gtgtTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGt                                                        >  1:1569212/1‑149 (MQ=255)
                                                                                         gtgtTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGt                                                        <  1:7533888/149‑1 (MQ=255)
                                                                                         gtgtTAAATTGGAACAATGGTTACAAGAAAATGACTACATTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGAAGCTAACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGt                                                        <  2:129066/149‑1 (MQ=255)
                                                                                           gtTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTAt                                                      <  2:3061974/149‑1 (MQ=255)
                                                                                            ttAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTAtt                                                     <  1:8342996/149‑1 (MQ=255)
                                                                                             tAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTa                                                    <  1:10151117/149‑1 (MQ=255)
                                                                                               aaTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACt                                                  >  1:3757778/1‑149 (MQ=255)
                                                                                                aTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATTGCTAAACAAGGTGCAACATTCTACGGTGTGGCTGTAGCACTTGCTCGTATTACTa                                                 >  2:2280497/1‑149 (MQ=255)
                                                                                                aTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTa                                                 >  2:1613216/1‑149 (MQ=255)
                                                                                                aTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTa                                                 >  1:6030717/1‑149 (MQ=255)
                                                                                                 ttGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTaa                                                <  1:2313244/149‑1 (MQ=255)
                                                                                                   ggAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAg                                              <  1:11023089/149‑1 (MQ=255)
                                                                                                    gAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATCAATTGTTAGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGATCGTATTACTAAAGc                                             >  2:4117509/1‑149 (MQ=255)
                                                                                                      aCAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCaa                                           <  2:8756393/149‑1 (MQ=255)
                                                                                                         aTGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTc                                        >  1:10888575/1‑149 (MQ=255)
                                                                                                         aTGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTc                                        >  1:619192/1‑149 (MQ=255)
                                                                                                         aTGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTAGTACTAAAGCAATTc                                        >  1:7360787/1‑149 (MQ=255)
                                                                                                          tGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTCCGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCt                                       <  2:7128236/149‑1 (MQ=255)
                                                                                                           ggTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCtt                                      <  1:9909564/149‑1 (MQ=255)
                                                                                                             ttCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTtga                                    <  1:1971627/149‑1 (MQ=255)
                                                                                                              tCCAAGAAAATGTCTACCTTAACGAGGCAGAAATCAAGTCATTGTTTGAGTCTGTAAGCGACGCAGCTTACGCACTCATCGCTAAAAAAGATGCATCATTCTACGGTGTGGTTGTAGACCTTGCTCGTATTACTAAAGCAATTCTtgat                                   <  2:4281005/149‑1 (MQ=255)
                                                                                                                cAAGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTtgatga                                 >  1:4652773/1‑149 (MQ=255)
                                                                                                                 aaGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTtgatgat                                <  2:3330525/149‑1 (MQ=255)
                                                                                                                  aGAAAATGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTtgatgatg                               <  2:3778787/149‑1 (MQ=255)
                                                                                                                     aaaTGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAAc                            <  2:3879595/149‑1 (MQ=255)
                                                                                                                      caTGACTACCTTAACGAAGCAGAACTCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAACAAGCTGCAACATTCTACTGTGTGGCTGTAGCCCTTGCTCGTATTCCTAAAGCAATTCTTGATGATGAACa                           >  2:10490344/2‑149 (MQ=255)
                                                                                                                        tGACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATg                         <  1:3909075/149‑1 (MQ=255)
                                                                                                                         gACTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAGAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGc                        >  1:6951523/1‑149 (MQ=255)
                                                                                                                          aCTACCTTAACGAAGCAGAAATCGATGAATTGTTTTAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCa                       >  2:9738754/1‑149 (MQ=255)
                                                                                                                          aCTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCa                       <  1:9780710/149‑1 (MQ=255)
                                                                                                                           cTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAg                      >  2:4832361/1‑149 (MQ=255)
                                                                                                                           cTACCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAg                      <  1:2807085/149‑1 (MQ=255)
                                                                                                                             aCCTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTa                    >  1:8186487/1‑149 (MQ=255)
                                                                                                                               cTTAACGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACt                  <  2:5309691/149‑1 (MQ=255)
                                                                                                                                   aCGAGGCAGAAAACGATGAATTGTTTGACTCTGGACGTGATGCAGCTTACTCAATCGTCGCTAAGAAAGGTGCAACATTCTACGGTGTGGCCGTCGTCCGTGCGCGTAGTACTAACGCAATTCTTCGTGGTGCACATGCAGCACTTCCt              >  2:6931075/1‑149 (MQ=255)
                                                                                                                                   aCGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTCCt              >  2:4479294/1‑149 (MQ=255)
                                                                                                                                   aCGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTCCt              >  2:3641844/1‑149 (MQ=255)
                                                                                                                                    cGAAGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTCCTg             >  1:6540275/1‑149 (MQ=255)
                                                                                                                                       aGCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTCCTGTAt          <  2:7515918/149‑1 (MQ=255)
                                                                                                                                        gCATAAATCGATGAATTGTTTAAGCATAGACGTGATGCAGCGTACTCAATCATCGCTAAAAAAGGCGCAACAGTCTACGGCGTGGCTGTAACCCTTTCTCGTATTACTAAAGCAATTCTTGATTATGAACATTCAGTACTTCCTGTATc         <  2:9225514/149‑1 (MQ=255)
                                                                                                                                        gCAGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTCCTGTATc         >  1:5441887/1‑149 (MQ=255)
                                                                                                                                          aGAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTCCTGTATCAg       >  2:7376299/1‑149 (MQ=255)
                                                                                                                                           gAAATCGATGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTCCTGTATCAGt      >  2:1791201/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTACTAAAGCAATTCTTGATGATGAACATGCAGTACTTCCTGTATCAGTATTC  >  AM406671/1081406‑1081697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑