Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 1,588,193 T→G Q297P (CAA→CCA)  ugd ← UDP‑glucose 6‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM4066711,588,1930TG98.7% 4119.3 / ‑4.3 1014Q297P (CAA→CCA) ugdUDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (10/3);  new base G (507/492);  total (518/496)
Fisher's exact test for biased strand distribution p-value = 9.12e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1025 total aligned reads displayed.

CATAATCTTTCAAATCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTTGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGTATTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACC  >  AM406671/1588047‑1588338
                                                                                                                                                  |                                                                                                                                                 
cATAATCTTTCAAATCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGga                                                                                                                                                 <  2:8351518/149‑1 (MQ=255)
    aTCTTTCAAATCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGAtt                                                                                                                                             <  2:5370972/149‑1 (MQ=255)
     tCTTTCAAATCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGAttt                                                                                                                                            <  1:10957623/149‑1 (MQ=255)
     tCTTTCAAATCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGAttt                                                                                                                                            >  1:1289621/1‑149 (MQ=255)
      cTTTCAAATCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTa                                                                                                                                           <  1:2858756/149‑1 (MQ=255)
        ttCAAATATAGTGTAGGTTCATAGATATGAACCGTTATCCCCTTATCTTGCTAGAGTTTCATCTCATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGc                                                                                                                                         <  2:9835286/149‑1 (MQ=255)
          cAAATCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCa                                                                                                                                       >  2:6910121/1‑149 (MQ=255)
          cAAATCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCa                                                                                                                                       <  1:7289427/149‑1 (MQ=255)
           aaaTCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCAt                                                                                                                                      <  1:10237098/149‑1 (MQ=255)
           aaaTCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCAt                                                                                                                                      <  2:4654256/149‑1 (MQ=255)
            aaTCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATCATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATa                                                                                                                                     <  1:3316713/149‑1 (MQ=255)
            aaTCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATa                                                                                                                                     >  1:7160393/1‑149 (MQ=255)
            aaTCTAGTGTAGGTTCATAGATAAGAACCGTTATCACCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACTCTGCTTTCATGACCAACCGTTAAATTTCAACAGTTTTTGGCGCTTTAGCCATa                                                                                                                                     >  2:3839158/1‑149 (MQ=255)
                ttgtgtAGGTTCATAGATAAAACCCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCACGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATActct                                                                                                                                 <  2:6508155/147‑1 (MQ=255)
                 aGTGTAGGTTCATAGGTAAGAACCTTTATCCCCTTATCTTTCAAGAATTTCATCACATCAATAATCGAACTCTGTCTTAAATTATCTGACCCAGCTTTCATGACAAACCGGTAAATTCCAACATTTTTTGGAGATTTAGCCATAATCTg                                                                                                                                <  2:11257318/149‑1 (MQ=255)
                 aGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTg                                                                                                                                >  1:3142406/1‑149 (MQ=255)
                 aGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTg                                                                                                                                <  1:3750926/149‑1 (MQ=255)
                 aGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATCACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTg                                                                                                                                >  1:8913786/1‑149 (MQ=255)
                  gtgtAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGc                                                                                                                               >  1:1599138/1‑149 (MQ=255)
                   tgtAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCt                                                                                                                              >  1:10386658/1‑149 (MQ=255)
                       ggTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCTAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTcaac                                                                                                                          >  1:6693300/1‑149 (MQ=255)
                        gTTCATAGATAAGAACCGTCATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCGGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGTTcaaca                                                                                                                         <  2:5565941/149‑1 (MQ=255)
                          tCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGATATTATCTGACCCAGCTTTCTTGACCAACCGGTAAATTCCAACCGTTTTTTGAGATTTAGCCATAATCTGCTcaacagt                                                                                                                       <  2:4268905/149‑3 (MQ=255)
                          tCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAAt                                                                                                                       <  2:676007/149‑1 (MQ=255)
                           cATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAACTTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTCTTTGGAGATTTAGCCATACTCTGCTCAACCAtt                                                                                                                      >  2:7270174/1‑148 (MQ=255)
                                aTAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTg                                                                                                                 <  2:10599863/149‑1 (MQ=255)
                                 tAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGt                                                                                                                <  2:1705812/149‑1 (MQ=255)
                                 tAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGt                                                                                                                <  1:3537701/149‑1 (MQ=255)
                                  aaGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGtt                                                                                                               >  2:2957718/1‑149 (MQ=255)
                                     aaCCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTc                                                                                                            <  2:4902955/149‑1 (MQ=255)
                                     aaCCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTc                                                                                                            >  1:5029932/1‑149 (MQ=255)
                                     aaCCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTc                                                                                                            <  2:1914719/149‑1 (MQ=255)
                                     aaCCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTc                                                                                                            >  2:10308208/1‑149 (MQ=255)
                                     aaCCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGAGTTAGCCATAATCTGCTCAACAATGTGCTGTTTTc                                                                                                            >  2:3566803/1‑149 (MQ=255)
                                     aaCCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCACCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGCTTTc                                                                                                            >  2:8554907/1‑149 (MQ=255)
                                        cGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTgg                                                                                                         >  1:8775547/1‑149 (MQ=255)
                                         gTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGt                                                                                                        >  1:1421890/1‑149 (MQ=255)
                                           tATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGttt                                                                                                      <  1:4682045/149‑1 (MQ=255)
                                           tATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGgtt                                                                                                      >  2:7518163/1‑149 (MQ=255)
                                            aTCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTc                                                                                                     >  1:234120/1‑149 (MQ=255)
                                              ccccTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGt                                                                                                   <  1:7464330/149‑1 (MQ=255)
                                              ccccTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGt                                                                                                   <  1:4381295/149‑1 (MQ=255)
                                               cccTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGtt                                                                                                  >  2:7941178/1‑149 (MQ=255)
                                               cccTGATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGtt                                                                                                  <  2:238664/149‑1 (MQ=255)
                                                  ttATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAAtgtg                                                                                                                    <  1:3004300/128‑1 (MQ=255)
                                                     tCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTgattt                                                                                            <  1:10585101/149‑5 (MQ=255)
                                                     tCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTgattt                                                                                            >  1:7047582/1‑145 (MQ=255)
                                                     tCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTgattt                                                                                            >  2:92310/1‑145 (MQ=255)
                                                      cTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTgatttg                                                                                           >  1:2629107/1‑144 (MQ=255)
                                                        tGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCTATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTgatttgac                                                                                         <  2:5845840/149‑8 (MQ=255)
                                                        tGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGAc                                                                                         >  1:5399279/1‑149 (MQ=255)
                                                          cAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACaa                                                                                       <  2:452220/149‑1 (MQ=255)
                                                             gagTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGATTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGGTTCGTTTGATTTGACAATaa                                                                                    >  1:1147363/1‑148 (MQ=255)
                                                                      cacatcaATAATCGAACTCTGTCTGAAATTATCTGACCTAGCTTTTATAACCAACCGGTAAATTCCAACAGTTGTTAGAGATTTAGCAATAATATGCTAAACAAGTTTCTGAATTCTAGTTTCGTTTGATTTGACAATTACCGtcatca                                                                           >  2:1096980/1‑149 (MQ=255)
                                                                      cacatcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGtcatca                                                                           >  1:5918608/1‑149 (MQ=255)
                                                                       acatcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCaa                                                                          <  1:9791379/149‑1 (MQ=255)
                                                                        catcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAAc                                                                         <  1:920568/149‑1 (MQ=255)
                                                                         atcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACt                                                                        <  2:1525315/149‑1 (MQ=255)
                                                                         atcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACt                                                                        <  2:711919/149‑1 (MQ=255)
                                                                                 tCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGAACAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGa                                                                >  2:6116547/1‑149 (MQ=255)
                                                                                  cGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGaa                                                               >  1:6516294/1‑149 (MQ=255)
                                                                                   gAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAAc                                                              >  2:8737268/1‑149 (MQ=255)
                                                                                     aCTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGc                                                            >  2:2073612/1‑149 (MQ=255)
                                                                                       tctgtctgAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGccc                                                          <  2:6315000/149‑1 (MQ=255)
                                                                                          gtctgAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCa                                                       >  1:9397530/1‑149 (MQ=255)
                                                                                                aaTTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTg                                                 >  1:7613526/1‑149 (MQ=255)
                                                                                                aaTTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTg                                                 <  2:5122855/149‑1 (MQ=255)
                                                                                                 aTTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAGTGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTgg                                                >  1:5610692/1‑149 (MQ=255)
                                                                                                 aTTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTgg                                                >  2:8145737/1‑149 (MQ=255)
                                                                                                 aTTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGATTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTgg                                                <  2:7668425/149‑1 (MQ=255)
                                                                                                    aTCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCa                                             >  1:9957550/1‑149 (MQ=255)
                                                                                                    aTCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCa                                             <  2:8917716/149‑1 (MQ=255)
                                                                                                       tGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCGGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCaaaa                                          >  1:2409828/1‑149 (MQ=255)
                                                                                                         aCCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAgc                                        >  2:3257746/1‑149 (MQ=255)
                                                                                                         aCCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAgc                                        <  2:2751699/149‑1 (MQ=255)
                                                                                                           ccAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAgctg                                      <  1:7740267/149‑1 (MQ=255)
                                                                                                            cAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCCACTCCTGAGGAACGCCCTCATAACTGGCCAAAAgctgc                                     >  2:4166601/1‑149 (MQ=255)
                                                                                                            cAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAgctgc                                     >  2:1310888/1‑149 (MQ=255)
                                                                                                             aGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAgctgct                                    >  2:3960544/1‑149 (MQ=255)
                                                                                                              gCTTTCATGGCCAACCGGTAAATCCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGGCCTCATAACTGGCCAAAAGCTGCtt                                   >  1:10730416/1‑149 (MQ=255)
                                                                                                              gCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCtt                                   <  1:4105654/149‑1 (MQ=255)
                                                                                                               cTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCttt                                  >  1:2193283/1‑149 (MQ=255)
                                                                                                                     tGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCt                            <  1:8359217/149‑1 (MQ=255)
                                                                                                                       aCCAACCGGTAAATTCCAACAGCCTTTGGCGATTTAGCCATAATCTGCTCAACAATGTCCTGGTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCCGCTGTGAGTCttt                          >  2:946728/1‑149 (MQ=255)
                                                                                                                         cAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCg                        >  1:1858205/1‑149 (MQ=255)
                                                                                                                         cAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCg                        >  1:9014818/1‑149 (MQ=255)
                                                                                                                            ccGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCa                     >  1:9584691/1‑149 (MQ=255)
                                                                                                                            ccGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCa                     >  1:6887595/1‑149 (MQ=255)
                                                                                                                            ccGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCa                     >  1:4852869/1‑149 (MQ=255)
                                                                                                                              ggTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCaaa                   >  1:6133087/1‑149 (MQ=255)
                                                                                                                               gTAAATTCCAACAGTTTTTGGAGATTTAGCCAGAATCTGCTCAACCATGTGCTGTTTTCTGGTTTCGTGTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCaacc                  >  2:1114157/1‑147 (MQ=255)
                                                                                                                                tAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACa                 >  1:8578972/1‑149 (MQ=255)
                                                                                                                                 aaaTTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACaa                >  2:10150459/1‑149 (MQ=255)
                                                                                                                                 aaaTTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACaa                >  1:10000247/1‑149 (MQ=255)
                                                                                                                                   aTTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATa              <  2:3446743/149‑1 (MQ=255)
                                                                                                                                    ttCCAACAGTTTTTGGAGATTTAGCCATNATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATaa             >  1:6345483/1‑149 (MQ=255)
                                                                                                                                     tCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAac            <  1:8231782/149‑1 (MQ=255)
                                                                                                                                       cAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAacca          >  1:5176291/1‑149 (MQ=255)
                                                                                                                                        aaCAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAaccac         <  2:9592778/149‑1 (MQ=255)
                                                                                                                                           aGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCAt      <  1:11149702/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CATAATCTTTCAAATCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTTGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGTATTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACC  >  AM406671/1588047‑1588338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑