Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 1,588,356 T→A N243Y (AAT→TAT)  ugd ← UDP‑glucose 6‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM4066711,588,3560TA99.8% 3881.5 / NA 991N243Y (AAT→TAT) ugdUDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base A (465/523);  minor base C (2/0);  total (467/524)
Fisher's exact test for biased strand distribution p-value = 2.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1042 total aligned reads displayed.

CTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGTATTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATTATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGCAAAAAGTTTGATTGCTTCT  >  AM406671/1588210‑1588501
                                                                                                                                                  |                                                                                                                                                 
  gCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAAtgt                                                                                                                                               >  2:2338688/1‑149 (MQ=255)
    tCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATgtgt                                                                                                                                             >  2:2635645/1‑149 (MQ=255)
      aactaTGTGCTGTTTTCTCGTTTCGTTTGCTTTTACAATAGTCGTCATCACCTGCTGATGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATACCCAGCTTTACCATAACTTGGATTATTATAATGTGTcc                                                                                                                                           <  2:6648154/149‑1 (MQ=255)
      aacaaTGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTcc                                                                                                                                           <  2:11352103/149‑1 (MQ=255)
      aacaaTGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTcc                                                                                                                                           <  2:6393019/149‑1 (MQ=255)
      aacaaTGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTcc                                                                                                                                           >  2:1655714/1‑149 (MQ=255)
          aTGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAAt                                                                                                                                       <  1:8509937/149‑1 (MQ=255)
          aTGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAAt                                                                                                                                       >  2:7794639/1‑149 (MQ=255)
            gtgCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATcc                                                                                                                                     <  2:7338335/149‑1 (MQ=255)
            gtgCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATcc                                                                                                                                     >  1:2313316/1‑149 (MQ=255)
                tGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCACAATCCGTGa                                                                                                                                 >  1:9448598/1‑149 (MQ=255)
                  ttttCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAAt                                                                                                                               >  1:8031531/1‑149 (MQ=255)
                  ttttCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAAt                                                                                                                               >  2:9515058/1‑149 (MQ=255)
                   tttCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATc                                                                                                                              <  2:131797/149‑1 (MQ=255)
                    ttCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATcc                                                                                                                             >  2:2811924/1‑149 (MQ=255)
                    ttCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATcc                                                                                                                             >  1:8353912/1‑149 (MQ=255)
                       tGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATccaac                                                                                                                          <  2:7160393/149‑1 (MQ=255)
                        ggTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATccaacc                                                                                                                         <  2:10662426/149‑1 (MQ=255)
                        ggTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATccaacc                                                                                                                         >  2:9699783/1‑149 (MQ=255)
                          tttCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAAACAAAACTTGGATTATTATAATGTGTCCCAATACGTGAATccaaccca                                                                                                                       >  2:3514467/1‑149 (MQ=255)
                             cGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAaca                                                                                                                    <  2:3626187/149‑1 (MQ=255)
                             cGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAaca                                                                                                                    <  2:10307479/149‑1 (MQ=255)
                              gtttgatttGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAacac                                                                                                                   <  2:4916962/142‑1 (MQ=255)
                               tttgatttGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATATCCAAAACGTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACAcc                                                                                                                  <  2:8268988/143‑1 (MQ=255)
                                ttgatttGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTGTTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCg                                                                                                                 >  1:8214715/6‑149 (MQ=255)
                                    tttGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTtaa                                                                                                             <  1:78171/148‑1 (MQ=255)
                                     ttGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTtaat                                                                                                            <  2:6045270/149‑1 (MQ=255)
                                     ttGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTAGGGAAACAATAACCACCATAACCAAAACTTGGATTATTAGAATGTGTCCCAAGTCGTGAATCCAACCCAACACCGTtaat                                                                                                            >  2:4267499/1‑149 (MQ=255)
                                      tGACAATAGCCGTCATCAACTCCTGATGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAANCCGTGAATCCAACCCAACACCGTtaata                                                                                                           <  1:6379060/149‑1 (MQ=255)
                                       gACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTtaataa                                                                                                          >  2:11252289/1‑149 (MQ=255)
                                       gACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGACTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTtaataa                                                                                                          >  2:3933725/1‑149 (MQ=255)
                                        aCAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTaataat                                                                                                         <  2:5931399/149‑1 (MQ=255)
                                          aaTAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAatat                                                                                                       >  1:10293078/1‑149 (MQ=255)
                                            tAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGt                                                                                                     <  2:5624319/149‑1 (MQ=255)
                                              gCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTg                                                                                                   <  1:2937939/149‑1 (MQ=255)
                                              gCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTg                                                                                                   <  1:7467876/149‑1 (MQ=255)
                                                 gTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACt                                                                                                <  2:7515841/149‑1 (MQ=255)
                                                   catcaACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTAt                                                                                              >  1:9262304/1‑149 (MQ=255)
                                                    atcaACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTAtt                                                                                             <  1:4582962/149‑1 (MQ=255)
                                                    atcaACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTAtt                                                                                             >  2:4789532/1‑149 (MQ=255)
                                                     tcaACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTAttt                                                                                            <  2:8025688/149‑1 (MQ=255)
                                                      caACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTa                                                                                           <  2:7018848/149‑1 (MQ=255)
                                                       aaCTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTaa                                                                                          >  2:4557709/1‑149 (MQ=255)
                                                         cTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAAtt                                                                                        <  1:1609357/149‑1 (MQ=255)
                                                             tGAGGAACGCCCTCATAACTGTCCAAAAGCTGCTTTGTGTCTTTCGGCAAACAATAACCACTATAACCAAACCTTGGATTATTATAATGTGTCCCTATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGAtt                                                                                    <  2:3142406/149‑1 (MQ=255)
                                                             tGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATATCCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGAtt                                                                                    <  2:9127668/149‑1 (MQ=255)
                                                               aGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGAtttt                                                                                  <  2:5253134/149‑1 (MQ=255)
                                                                       ccTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCa                                                                          <  2:5393511/149‑1 (MQ=255)
                                                                        cTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCAt                                                                         >  1:6129332/1‑149 (MQ=255)
                                                                        cTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCAt                                                                         >  1:5453305/1‑149 (MQ=255)
                                                                          cATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATaa                                                                       <  2:8486979/149‑1 (MQ=255)
                                                                             aaCTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTATCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTa                                                                    >  1:6984450/1‑149 (MQ=255)
                                                                              aCTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTAt                                                                   <  1:4178940/149‑1 (MQ=255)
                                                                                tGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGACTCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCa                                                                 <  1:6842647/149‑1 (MQ=255)
                                                                                      aaaGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCa                                                           >  1:8638120/1‑149 (MQ=255)
                                                                                           tgctTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTaaa                                                      <  1:10052595/149‑1 (MQ=255)
                                                                                           tgctTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTaaa                                                      >  1:10848276/1‑149 (MQ=255)
                                                                                            gctTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCATCCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTaaag                                                     <  2:8617378/149‑1 (MQ=255)
                                                                                             ctTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTaaaga                                                    <  1:9181580/149‑1 (MQ=255)
                                                                                             ctTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTaaaga                                                    <  2:3307449/149‑1 (MQ=255)
                                                                                              tttGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTaaagaa                                                   <  1:10669360/149‑1 (MQ=255)
                                                                                               ttGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTaaagaaa                                                  >  1:5116077/1‑149 (MQ=255)
                                                                                                tGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTaaagaaag                                                 <  2:10157249/149‑1 (MQ=255)
                                                                                                 gAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATGAGTATCAAGTTCATTAAAGAAAGc                                                <  1:8837592/149‑1 (MQ=255)
                                                                                                  aGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACTCCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGcc                                               <  1:7150410/149‑1 (MQ=255)
                                                                                                      tttCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCAcgc                                           >  1:4350473/1‑149 (MQ=255)
                                                                                                        tCGGCAAACAACAACCCCCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACgcgc                                         <  2:202601/149‑1 (MQ=255)
                                                                                                         cGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCa                                        <  2:6806941/149‑1 (MQ=255)
                                                                                                         cGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCa                                        <  2:881921/149‑1 (MQ=255)
                                                                                                          ggCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCAt                                       <  2:511717/149‑1 (MQ=255)
                                                                                                          ggCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCAt                                       <  1:11162484/149‑1 (MQ=255)
                                                                                                           gCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATGAAGTTCATTAAAGAAAGCCACGCGCATc                                      >  1:1176743/1‑149 (MQ=255)
                                                                                                           gCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATc                                      >  2:1495311/1‑149 (MQ=255)
                                                                                                             aaaCAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGc                                    >  1:1351493/1‑149 (MQ=255)
                                                                                                               aCAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCaa                                  <  1:1854815/149‑1 (MQ=255)
                                                                                                               aCAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCaa                                  <  1:67005/149‑1 (MQ=255)
                                                                                                                 aaTAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAagg                                >  2:6217753/1‑149 (MQ=255)
                                                                                                                   aaaCCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCACCCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAaggta                              <  1:347929/148‑1 (MQ=255)
                                                                                                                      ccaccaTAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAaggtaggt                           >  1:9728815/1‑149 (MQ=255)
                                                                                                                      ccaccaTAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAaggtaggt                           >  2:1003238/1‑149 (MQ=255)
                                                                                                                       caccaTAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAggtaggta                          <  2:6479329/149‑1 (MQ=255)
                                                                                                                        accaTAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTAt                         >  1:479554/1‑149 (MQ=255)
                                                                                                                         ccaTAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTAtt                        >  1:7692490/1‑149 (MQ=255)
                                                                                                                         ccaTAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTAtt                        >  1:2696442/1‑149 (MQ=255)
                                                                                                                         ccaTAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTAtt                        <  1:3940603/149‑1 (MQ=255)
                                                                                                                         ccaTAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATAAGATGACTATTTAATTGATTTTTTTCGGCATAACTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTAtt                        >  2:295755/1‑149 (MQ=255)
                                                                                                                          caTAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTa                       <  1:10453060/149‑1 (MQ=255)
                                                                                                                             aaCCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCAGCGCAAGGTAGGTATTAGCa                    >  1:5634822/1‑149 (MQ=255)
                                                                                                                             aaCCAAAACTTGGATTATTATAATGTATCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAACTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGCa                    <  1:7318053/149‑1 (MQ=255)
                                                                                                                              aCCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGCaa                   <  2:10470867/149‑1 (MQ=255)
                                                                                                                               ccAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTg                                                                                       <  2:9428134/80‑1 (MQ=255)
                                                                                                                                  aaaCTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGCAAAAAg               >  1:6572158/1‑149 (MQ=255)
                                                                                                                                   aaCTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGCAAAAAGt              >  1:3158092/1‑149 (MQ=255)
                                                                                                                                    aCTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGCAAAAAGtt             <  2:10804454/149‑1 (MQ=255)
                                                                                                                                    aCTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGCAAAAAGtt             >  2:8970825/1‑149 (MQ=255)
                                                                                                                                      ttGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGCAAAAAGTTTg           <  1:3670998/149‑1 (MQ=255)
                                                                                                                                       tGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATGAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGCACAAAGTTTGa          <  1:5916125/149‑1 (MQ=255)
                                                                                                                                          attattataATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGCAAAAAGTTTGATTg       >  1:2106304/1‑149 (MQ=255)
                                                                                                                                            tattataatGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGCAAAAAGTTTGATTGCt     <  2:1094900/149‑1 (MQ=255)
                                                                                                                                               tataatGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGCAAAAAGTTTGATTGCTTCt  <  1:5270411/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGTATTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATTATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGCAAAAAGTTTGATTGCTTCT  >  AM406671/1588210‑1588501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑