Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 447,854 G→A A98T (GCT→ACT)  llmg_0454 → putative beta‑glucosides‑specific IIBC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM406671447,8540GA99.9% 5520.9 / NA 1429A98T (GCT→ACT) llmg_0454putative beta‑glucosides‑specific IIBC component
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (689/738);  minor base C (1/0);  total (690/739)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1450 total aligned reads displayed.

TACCTGGTGTCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTGCTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGATTATTAAGTATCTT  >  AM406671/447708‑447999
                                                                                                                                                  |                                                                                                                                                 
tACCTGGTGTCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTtact                                                                                                                                                 <  1:10126763/149‑1 (MQ=255)
tACCTGGTGTCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTtact                                                                                                                                                 >  1:3726402/1‑149 (MQ=255)
 aCCTGGTGTCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTtactg                                                                                                                                                <  1:100022/149‑1 (MQ=255)
  ccTGGTGTCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTtactgc                                                                                                                                               <  1:3888276/149‑1 (MQ=255)
    tGGTGTCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCaa                                                                                                                                             <  2:2936903/149‑1 (MQ=255)
    tGGTGTCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCaa                                                                                                                                             <  2:1087857/149‑1 (MQ=255)
    tGGTGTCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCaa                                                                                                                                             <  1:2516079/149‑1 (MQ=255)
      gtgtCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACa                                                                                                                                           <  1:8739483/149‑1 (MQ=255)
        gtCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGc                                                                                                                                         >  1:218534/1‑149 (MQ=255)
         tCGCTGGATCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACGTACTGCAACAGCa                                                                                                                                        <  1:11061071/149‑1 (MQ=255)
         tCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCa                                                                                                                                        <  2:9862789/149‑1 (MQ=255)
           gCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTTTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCAcc                                                                                                                                      <  1:7339122/149‑1 (MQ=255)
           gCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCAcc                                                                                                                                      <  1:8971836/149‑1 (MQ=255)
             tGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCgg                                                                                                                                    >  1:11368028/1‑149 (MQ=255)
             tGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACAgg                                                                                                                                    <  1:3101512/149‑1 (MQ=255)
                 gCTGTTTATAACTCAAACTTGAATCAATAACAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAg                                                                                                                                <  2:6687706/149‑1 (MQ=255)
                  cTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGt                                                                                                                               >  1:1808270/1‑149 (MQ=255)
                  cTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGt                                                                                                                               <  1:6123937/149‑1 (MQ=255)
                   tGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTg                                                                                                                              <  1:581407/149‑1 (MQ=255)
                    gTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTgg                                                                                                                             <  1:4193581/149‑1 (MQ=255)
                     tttATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGt                                                                                                                            <  2:7569575/149‑1 (MQ=255)
                          aaCTCAAACTTGAATCAATACCAAGTAGTGATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCGACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTaaaaa                                                                                                                       >  2:8065145/1‑149 (MQ=255)
                           aCTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGCACAGCTTGGAGATTCAGTTGTTGATGAAGATGGAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGCGGTaaaaaa                                                                                                                      >  2:5183149/1‑149 (MQ=255)
                           aCTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTaaaaaa                                                                                                                      <  1:2327215/149‑1 (MQ=255)
                            cTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAAc                                                                                                                     <  1:7847940/149‑1 (MQ=255)
                            cTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAAc                                                                                                                     <  1:9298753/149‑1 (MQ=255)
                             tCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACa                                                                                                                    >  1:1612476/1‑149 (MQ=255)
                               aaaCTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACaaa                                                                                                                  >  1:3326162/1‑149 (MQ=255)
                                 aCTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAAt                                                                                                                >  1:810009/1‑149 (MQ=255)
                                  cTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATc                                                                                                               >  1:5577943/1‑149 (MQ=255)
                                      aaTCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAAt                                                                                                           >  1:11200147/1‑149 (MQ=255)
                                         cAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGt                                                                                                        >  2:8956769/1‑149 (MQ=255)
                                          aaTATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGtt                                                                                                       <  2:1926004/149‑1 (MQ=255)
                                           atatCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTc                                                                                                      >  1:8555223/1‑149 (MQ=255)
                                             atCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCAt                                                                                                    >  1:1039111/1‑149 (MQ=255)
                                                aaGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCt                                                                                                 >  2:9330225/1‑149 (MQ=255)
                                                aaGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCt                                                                                                 <  2:7764639/149‑1 (MQ=255)
                                                  gtagtTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCttt                                                                                               <  2:2416493/149‑1 (MQ=255)
                                                   tagtTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTAGTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTc                                                                                              >  2:1691762/1‑149 (MQ=255)
                                                   tagtTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTc                                                                                              <  2:8763231/149‑1 (MQ=255)
                                                    agtTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTcc                                                                                             >  1:9788118/1‑149 (MQ=255)
                                                     gtTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCa                                                                                            >  2:1466059/1‑149 (MQ=255)
                                                     gtTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCa                                                                                            <  1:9992724/149‑1 (MQ=255)
                                                       tATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAg                                                                                          <  1:1996683/149‑1 (MQ=255)
                                                        aTTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGt                                                                                         >  1:10264313/1‑149 (MQ=255)
                                                        aTTGGACAAGCTGTAGAAGATGTGTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGt                                                                                         <  2:5648735/149‑1 (MQ=255)
                                                         ttGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTg                                                                                        <  1:4955277/149‑1 (MQ=255)
                                                          tGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGCACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCAAAGTgg                                                                                       >  2:9158070/1‑149 (MQ=255)
                                                           ggACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGt                                                                                      >  1:2934109/1‑149 (MQ=255)
                                                                 gCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTgg                                                                                <  2:4580346/149‑1 (MQ=255)
                                                                 gCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTgg                                                                                <  1:3577996/149‑1 (MQ=255)
                                                                      agaagaTGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACaa                                                                           >  1:9138621/1‑149 (MQ=255)
                                                                      agaagaTGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACaa                                                                           >  1:4313087/1‑149 (MQ=255)
                                                                        aagaTGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTAGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAAtt                                                                         <  2:6445546/149‑1 (MQ=255)
                                                                        aagaTATGTATGGCGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGAGCAAGCACTTACTGCAACAGCACAGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAAtt                                                                         <  2:4148518/149‑1 (MQ=255)
                                                                           aTGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACa                                                                      <  1:7557307/149‑1 (MQ=255)
                                                                           aTGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACa                                                                      >  2:5499776/1‑149 (MQ=255)
                                                                           aTGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACa                                                                      >  2:5064038/1‑149 (MQ=255)
                                                                              tttATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTNCCAAGTGGTTATTGGGACAATTACAGGt                                                                   <  1:6337383/149‑1 (MQ=255)
                                                                                    aCGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCgatg                                                             <  2:9712621/149‑1 (MQ=255)
                                                                                    aCGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCgatg                                                             <  2:2602256/149‑1 (MQ=255)
                                                                                       aGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGAtt                                                          <  1:9540426/149‑1 (MQ=255)
                                                                                        ggTTGTTGAACAGCTTGGAGATTCAGTTATTGATGAAGATGCACCGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTc                                                         <  1:8265416/149‑1 (MQ=255)
                                                                                         gttgttGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTcc                                                        >  2:6945437/1‑149 (MQ=255)
                                                                                         gttgttGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTcc                                                        <  2:3860502/149‑1 (MQ=255)
                                                                                          ttgttgAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCa                                                       <  1:5289067/149‑1 (MQ=255)
                                                                                           tgttgAGCAGCATGGAGATTCAGTTGTTGATGAGAAGACAGCGGCGCAAGCACTTACTGCACCAGCACCGGGTAGTGGTAAAAAACAAAATCCAATTGTTCATGCCTTCCAAGTGGTTATTTGGCAAGTTACAGGTTCGATGATGCCac                                                      >  2:5389719/1‑148 (MQ=255)
                                                                                             ttgAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAatt                                                    >  1:10763363/1‑149 (MQ=255)
                                                                                             ttgAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAatt                                                    >  2:1293393/1‑149 (MQ=255)
                                                                                               gAAAAGCTTGGAGATTCAGTTGTTGATGAAGATGCATCGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAattat                                                  <  2:9556655/149‑1 (MQ=255)
                                                                                                aaCAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAattatt                                                 <  2:7469394/149‑1 (MQ=255)
                                                                                                aaCAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCGAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAattatt                                                 <  1:4554950/149‑1 (MQ=255)
                                                                                                    gCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGtt                                             <  2:717340/149‑1 (MQ=255)
                                                                                                      taggAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTAGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTa                                           <  2:8249203/147‑1 (MQ=255)
                                                                                                       tGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTAc                                          >  2:2260614/1‑149 (MQ=255)
                                                                                                       tGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTAc                                          <  2:7959766/149‑1 (MQ=255)
                                                                                                        ggAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACt                                         <  1:277374/149‑1 (MQ=255)
                                                                                                            aTTCAGTTGTTGATGAAGATGCTACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTATTTGTGCTTTCCCGGTGGTTATTGGGACAATTCCGGGTTTGCTGATTCCCATTATTGCTTTCCTcgcg                                     >  2:4017591/1‑149 (MQ=255)
                                                                                                              tCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGc                                   <  1:10603754/149‑1 (MQ=255)
                                                                                                                aGTTGGTGGTGAAGATGCAACGGCGCAAGCACTTACTGCAACAGGACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCtg                                 <  2:4353585/149‑1 (MQ=255)
                                                                                                                  ttgttgATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACCGGTTCGATGATTCCACTTATTGGTTTACTTGCGGCtggt                               >  1:481254/1‑149 (MQ=255)
                                                                                                                   tgttgATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCtggtg                              >  2:34094/1‑149 (MQ=255)
                                                                                                                    gttgATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCtggtgg                             <  2:10257321/149‑1 (MQ=255)
                                                                                                                     ttgATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCACGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTggg                            <  1:6857825/149‑1 (MQ=255)
                                                                                                                     ttgATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTggg                            >  1:1373449/1‑149 (MQ=255)
                                                                                                                     ttgATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTggg                            <  2:11262240/149‑1 (MQ=255)
                                                                                                                       gatgaAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGgat                          >  2:11076389/1‑149 (MQ=255)
                                                                                                                       gatgaAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGgat                          >  2:1049915/1‑149 (MQ=255)
                                                                                                                       gatgaAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGgat                          >  2:3186997/1‑149 (MQ=255)
                                                                                                                          gaAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGgatgat                       >  2:590615/1‑149 (MQ=255)
                                                                                                                              aTGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAAt                   >  1:10928707/1‑149 (MQ=255)
                                                                                                                                  aaCTGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGat               <  2:211063/149‑1 (MQ=255)
                                                                                                                                  aaCGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGat               <  2:5777314/149‑1 (MQ=255)
                                                                                                                                  aaCGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGat               <  1:7069210/149‑1 (MQ=255)
                                                                                                                                  aaCGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGat               >  2:10659960/1‑149 (MQ=255)
                                                                                                                                     ggCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGattat            >  2:8515859/1‑149 (MQ=255)
                                                                                                                                          aaGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGATTATTAAGt       <  1:6614997/149‑1 (MQ=255)
                                                                                                                                            gCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTTTTCATGCTTTCCAAGTGGTTATTCGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGACTGGTGGGATGATTAATGGATTATTCAGTAt     >  2:4794496/1‑149 (MQ=255)
                                                                                                                                            gCACGTACTGCAACAGCGGCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGATTATTAAGTAt     <  2:6130220/149‑1 (MQ=255)
                                                                                                                                             cACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGATTATTAAGTATc    <  2:3595695/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TACCTGGTGTCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTGCTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGATTATTAAGTATCTT  >  AM406671/447708‑447999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑