Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 1,783,142 G→A A603V (GCA→GTA)  llmg_1803 ← putative secreted protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM4066711,783,1420GA99.8% 3928.0 / NA 1028A603V (GCA→GTA) llmg_1803putative secreted protein
Reads supporting (aligned to +/- strand):  ref base G (0/2);  major base A (486/537);  minor base C (2/0);  total (488/540)
Fisher's exact test for biased strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1028 total aligned reads displayed.

GGATTTTATTAGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTGCCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGCCATTAGTGATAGACGT  >  AM406671/1782996‑1783287
                                                                                                                                                  |                                                                                                                                                 
  aTTTTATTAGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGt                                                                                                                                               <  1:10817947/149‑1 (MQ=255)
  aTTTTATTAGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGt                                                                                                                                               >  1:8945049/1‑149 (MQ=255)
  aTTTTATTAGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGt                                                                                                                                               <  1:2671248/149‑1 (MQ=255)
     ttattaGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTtg                                                                                                                                            >  1:4385993/1‑149 (MQ=255)
     ttattaGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTtg                                                                                                                                            <  1:2933932/149‑1 (MQ=255)
      tattaGCCACTGTCTCTGTGGAACCTGTATTCCCGCCAGTTGATGGCGTGACATTTGCTCCTGTCGTCCCTCTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGGGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCCTTtgt                                                                                                                                           <  2:2535201/149‑1 (MQ=255)
      tattaGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTtgt                                                                                                                                           >  2:5750640/1‑149 (MQ=255)
       attaGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTtgtg                                                                                                                                          >  2:598409/1‑149 (MQ=255)
       attaGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTtgtg                                                                                                                                          <  2:4193728/149‑1 (MQ=255)
           gCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGtgctgc                                                                                                                                      <  2:2903179/149‑1 (MQ=255)
             cACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATATCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGtgctgctg                                                                                                                                    <  1:6733162/149‑1 (MQ=255)
             cACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGtgctgctg                                                                                                                                    <  1:5586993/149‑1 (MQ=255)
                tGTCGCTGTGGATGCTGTATTCCAGCTAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGctgctgctg                                                                                                                                 <  1:7348498/149‑1 (MQ=255)
                tGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGctgctgctg                                                                                                                                 >  1:806104/1‑149 (MQ=255)
                 gTCGCTGTGGAGGCTGTATTCCAGCCAGTTGGTGGGGTGACATTTGCTCCTGTCGGCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGt                                                                                                                                <  2:3360098/149‑1 (MQ=255)
                  tCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTc                                                                                                                               <  1:2090625/149‑1 (MQ=255)
                     cTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGc                                                                                                                            >  2:10576951/1‑149 (MQ=255)
                        tgGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTg                                                                                                                         <  2:9796680/149‑1 (MQ=255)
                         ggATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGa                                                                                                                        <  2:804268/149‑1 (MQ=255)
                           aTGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGAcc                                                                                                                      <  2:3643011/149‑1 (MQ=255)
                              cTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGta                                                                                                                   <  2:10896842/149‑1 (MQ=255)
                               tGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGtat                                                                                                                  <  2:2176207/149‑1 (MQ=255)
                                 tATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAg                                                                                                                <  1:7488048/149‑1 (MQ=255)
                                 tATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAg                                                                                                                <  1:10594718/149‑1 (MQ=255)
                                 tATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGCGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAg                                                                                                                <  2:6840036/149‑1 (MQ=255)
                                  aTTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGt                                                                                                               <  1:8607371/149‑1 (MQ=255)
                                   ttCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTCGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCCGCGTGTCCGGATAGtt                                                                                                              >  2:4702528/1‑149 (MQ=255)
                                     ccagccagTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTAt                                                                                                            >  1:8746258/1‑149 (MQ=255)
                                      cagccagTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTAtt                                                                                                           >  1:4687099/1‑149 (MQ=255)
                                       agccagTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGNTGCTGTCAGCGTGACCGTATAGTTATTg                                                                                                          <  1:6350131/149‑1 (MQ=255)
                                        gccagTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGt                                                                                                         <  1:3588155/149‑1 (MQ=255)
                                           agTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGttt                                                                                                       >  2:4042002/1‑148 (MQ=255)
                                             ttGGTGGTGTGACATTTGCTCCTGTCGTCCCACTGGCTGACAGGCGAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGGCGTAGTTGAAGGTTATTGTTTGTGCTGCTGTTGTTAGGGTGACCGTATAGTTATTGTTTGCt                                                                                                    >  2:7548492/1‑149 (MQ=255)
                                              tggtggTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCtt                                                                                                   <  1:388552/149‑1 (MQ=255)
                                               ggtggtGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTg                                                                                                  <  2:1713041/149‑1 (MQ=255)
                                                gtggtgTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTgg                                                                                                 <  1:823840/149‑1 (MQ=255)
                                                gtggtgTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTgg                                                                                                 <  1:5766596/149‑1 (MQ=255)
                                                 tggtgTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCCAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTggg                                                                                                >  1:11284265/1‑149 (MQ=255)
                                                   gtgtGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGtt                                                                                              >  1:4881643/1‑149 (MQ=255)
                                                    tgtgACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTg                                                                                             <  2:480093/149‑1 (MQ=255)
                                                    tgtgACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGAAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGGATAGTTATTGTTTGCTTGGGTTg                                                                                             >  2:7730254/1‑149 (MQ=255)
                                                       gACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCGGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTgcgc                                                                                          >  2:8400363/1‑149 (MQ=255)
                                                         cacttGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTa                                                                                        <  1:7635339/146‑1 (MQ=255)
                                                         cATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTAGAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTa                                                                                        <  1:5039275/149‑1 (MQ=255)
                                                          aTTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTaa                                                                                       <  2:3190719/149‑1 (MQ=255)
                                                           tttGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAAc                                                                                      >  1:1196489/1‑149 (MQ=255)
                                                            ttGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACt                                                                                     >  1:1835621/1‑149 (MQ=255)
                                                             tGCTCCTGTCGTCCCTCTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGTGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTg                                                                                    <  2:8679210/149‑1 (MQ=255)
                                                             tGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTCAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTg                                                                                    >  2:9295099/1‑149 (MQ=255)
                                                                  cTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTgttggg                                                                               >  2:8984083/1‑149 (MQ=255)
                                                                  cTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTgttggg                                                                               <  1:536281/149‑1 (MQ=255)
                                                                   tGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTgttgggt                                                                              <  1:6930421/149‑1 (MQ=255)
                                                                    gtcgtcCCACTCGCTGACAGGCTAGTAGGCATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTgttgggtt                                                                             >  2:7067341/1‑149 (MQ=255)
                                                                     tcgtcCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTgttgggttg                                                                            <  2:8175209/149‑1 (MQ=255)
                                                                     tcgtcCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTgttgggttg                                                                            >  1:3332468/1‑149 (MQ=255)
                                                                      cgtcCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTgttgggttgg                                                                           >  2:298208/1‑149 (MQ=255)
                                                                      cgtcCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTgttgggttgg                                                                           >  2:652530/1‑149 (MQ=255)
                                                                       gtcCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGc                                                                          <  1:4001022/149‑1 (MQ=255)
                                                                         cccACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCg                                                                        <  1:7837457/149‑1 (MQ=255)
                                                                            aCTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCtg                                                                     <  1:1019997/149‑1 (MQ=255)
                                                                            aCTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCtg                                                                     >  1:651648/1‑149 (MQ=255)
                                                                                    aCAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGtt                                                             >  2:887213/1‑149 (MQ=255)
                                                                                          tagtagGTATAGCAGGCGTGTTGAGTGCTCCTGTGACGTAGCCATAGTTGAATGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGAGTTGGCCGCTGTGAAAGTTGCCAgt                                                       <  2:1189141/149‑1 (MQ=255)
                                                                                           agtagGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAgtg                                                      <  1:11062406/149‑1 (MQ=255)
                                                                                                gTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCt                                                 >  2:2737239/1‑149 (MQ=255)
                                                                                                    aGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTATCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTCTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAg                                             <  2:6373082/149‑1 (MQ=255)
                                                                                                      cAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGtt                                           <  1:410881/149‑1 (MQ=255)
                                                                                                      cAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGtt                                           <  1:3194443/149‑1 (MQ=255)
                                                                                                        ggCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGt                                         <  2:4564027/149‑1 (MQ=255)
                                                                                                          cGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCg                                       <  1:9245408/149‑1 (MQ=255)
                                                                                                            tgtTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCa                                     >  2:1943219/1‑149 (MQ=255)
                                                                                                            tgtTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCa                                     >  1:8460532/1‑149 (MQ=255)
                                                                                                            tgtTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCa                                     <  2:9091041/149‑1 (MQ=255)
                                                                                                            tgtTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCa                                     >  2:11219208/1‑149 (MQ=255)
                                                                                                             gtTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCAc                                    >  2:5490663/1‑149 (MQ=255)
                                                                                                               tGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCCCCa                                  >  1:241945/1‑149 (MQ=255)
                                                                                                                 ggTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGt                                <  2:1462340/148‑1 (MQ=255)
                                                                                                                    gCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGtata                             <  2:96043/149‑1 (MQ=255)
                                                                                                                    gCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGtata                             >  1:525221/1‑149 (MQ=255)
                                                                                                                    gCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGtata                             <  1:5981095/149‑1 (MQ=255)
                                                                                                                       ccTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGt                          <  1:10075712/149‑1 (MQ=255)
                                                                                                                        cTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTa                         >  2:3803277/1‑149 (MQ=255)
                                                                                                                         tGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTAc                        <  2:5247884/149‑1 (MQ=255)
                                                                                                                         tGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTAc                        <  1:971248/149‑1 (MQ=255)
                                                                                                                          gagaCGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACt                       >  2:10137330/1‑149 (MQ=255)
                                                                                                                           agaCGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTc                      <  2:5021874/149‑1 (MQ=255)
                                                                                                                           agaCGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTc                      >  2:7199506/1‑149 (MQ=255)
                                                                                                                              cGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTg                   <  1:9058465/149‑1 (MQ=255)
                                                                                                                              cGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTg                   <  1:1401634/149‑1 (MQ=255)
                                                                                                                                tAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGcc                 >  1:7184923/1‑149 (MQ=255)
                                                                                                                                  gCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGCCAt               <  1:9565813/149‑1 (MQ=255)
                                                                                                                                   ccATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGGTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGATGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGCCGtt              >  2:10745701/1‑149 (MQ=255)
                                                                                                                                    cATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCCGTATAAGTACTCGTGCCATTa             >  1:170165/1‑149 (MQ=255)
                                                                                                                                    cATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGCCATTa             >  2:6181231/1‑149 (MQ=255)
                                                                                                                                     aTAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGCCATTAg            <  2:10373251/149‑1 (MQ=255)
                                                                                                                                      tAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGCCATTAGt           >  1:10383706/1‑149 (MQ=255)
                                                                                                                                       aGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGCCATTAGTg          <  1:6199313/149‑1 (MQ=255)
                                                                                                                                          tGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGCCATTAGTGATa       >  1:3706739/1‑149 (MQ=255)
                                                                                                                                           gAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGCCATTAGTGATAg      >  1:9985011/1‑149 (MQ=255)
                                                                                                                                              ggTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGCCATTAGTGATAGACg   >  2:4399215/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGATTTTATTAGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTGCCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGCCATTAGTGATAGACGT  >  AM406671/1782996‑1783287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑