Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 1,826,407 A→G Y12Y (TAT→TAC)  llmg_1845 ← metal‑dependent hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM4066711,826,4070AG99.8% 5014.6 / NA 1235Y12Y (TAT→TAC) llmg_1845metal‑dependent hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (563/668);  minor base C (0/2);  total (565/670)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1238 total aligned reads displayed.

TAATGGCTCAAAATAACCGCATCCAACTGAGTCGGTTGTAACTCATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGATACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTA‑TTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACGTCCTATATTATTCTAAATGGCACTTATCCACTTCAATTAT  >  AM406671/1826261‑1826551
                                                                                                                                                  |                                                                                                                                                   
 aaTGGCTCAAAATAACCGCATCCAACTGAGTCGGTTGTAACTCATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTAc                                                                                                                                                  >  1:1639928/1‑149 (MQ=255)
   tGGCTCAAAATAACCGCATCCAACTGAGTCGGTTGTAACTCATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTAccc                                                                                                                                                >  2:10186839/1‑149 (MQ=255)
    ggCTCAAAATAACCGCATCCAACTGAGTCGGTTGTAACTCATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCt                                                                                                                                               >  2:880688/1‑149 (MQ=255)
      cTCAAAATAACCGCATCCAACTGAGTCGGTTGTAACTCATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTcc                                                                                                                                             >  1:1275667/1‑149 (MQ=255)
             tAACCGCATCCAACTGAGTCGGTTGTAACTCATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAaca                                                                                                                                      >  2:2087647/1‑149 (MQ=255)
             tAACCGCATCCAACTGAGTCGGTTGTAACTCATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAaca                                                                                                                                      <  2:4865944/149‑1 (MQ=255)
                     tCCAACTGAGTCGGTTGTAACTCATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAgcgc                                                                                                                              <  1:3231210/149‑1 (MQ=255)
                      ccAACTGAGTCGGTTGTAACTCATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCt                                                                                                                             >  1:2755247/1‑149 (MQ=255)
                      ccAACTGAGTCGGTTGTAACTCATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCt                                                                                                                             <  1:8152820/149‑1 (MQ=255)
                      ccAACTGAGTCGGTTGTAACTCATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCt                                                                                                                             <  1:1746056/149‑1 (MQ=255)
                       cAACTGAGTCGGTTGTAACTCATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTg                                                                                                                            <  2:10398048/149‑1 (MQ=255)
                         aCTGAGTCGGTTGTAACTCATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGtt                                                                                                                          <  1:3675914/149‑1 (MQ=255)
                          cTGAGTCGGTTGTAACTCATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTa                                                                                                                         >  2:3420725/1‑149 (MQ=255)
                              gTCGGTTGTAACTCATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAAttt                                                                                                                     <  2:5991860/149‑1 (MQ=255)
                              gTCGGTTGTAACTCATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTAACCTCCCCAAACACCTAGCGCTGTTAAttt                                                                                                                     <  2:7052314/149‑1 (MQ=255)
                               tCGGTTGTAACTTATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTc                                                                                                                    >  1:2922778/1‑149 (MQ=255)
                                cGGTTGTAACTCATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCa                                                                                                                   <  1:658222/149‑1 (MQ=255)
                                cGGTTGTAACTCATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCa                                                                                                                   >  1:6219770/1‑149 (MQ=255)
                                  gTTGTAACTCATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCAtt                                                                                                                 >  2:3644860/1‑149 (MQ=255)
                                   ttGTAACTCATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCAttt                                                                                                                >  1:8980171/1‑149 (MQ=255)
                                    tGTAACTCATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCATGCATCACGAGTGGGGTACCCTCCCCAAACACCAAGCGCTGTTAATTTCAtttt                                                                                                               >  1:8847256/1‑149 (MQ=255)
                                     gTAACTCATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCAttttt                                                                                                              <  2:9513185/149‑1 (MQ=255)
                                          tCATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTtctc                                                                                                         <  1:10995796/149‑1 (MQ=255)
                                          tCATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGGCTAACAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCGCCAAACACCTAGCGCTGTTAATTTCATTTTTTtctc                                                                                                         >  2:7688895/1‑149 (MQ=255)
                                           cATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTtctct                                                                                                        <  1:5969302/149‑1 (MQ=255)
                                            aTGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTtctctc                                                                                                       <  2:4695339/149‑1 (MQ=255)
                                              gTTCAAGCTCAGTCACCGCACGAGACCCGCAGGCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAGAAGATAAGAAGTTGTTCCTGCATCACGGGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATGTTTTTCTCTCtt                                                                                                     <  2:2777146/149‑1 (MQ=255)
                                               ttCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCttt                                                                                                    <  1:8588994/149‑1 (MQ=255)
                                                tCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTc                                                                                                   <  1:64232/149‑1 (MQ=255)
                                                 cAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAACAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCt                                                                                                  >  2:10573356/1‑149 (MQ=255)
                                                 cAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCt                                                                                                  >  2:8987926/1‑149 (MQ=255)
                                                  aaGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTa                                                                                                 <  1:5715508/149‑1 (MQ=255)
                                                  aaGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTa                                                                                                 <  2:8560766/149‑1 (MQ=255)
                                                   aGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTTCCCTCCTCATTCACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTA‑t                                                                                               <  2:9258549/149‑1 (MQ=255)
                                                       cAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTA‑ttt‑tt                                                                                          >  1:976276/1‑149 (MQ=255)
                                                          tCACCGCACGATACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTATCGCTGTTAATTTCATTTTTTTCTCTCTTTCTA‑ttt‑tt‑ttc                                                                                      <  2:1506963/149‑3 (MQ=255)
                                                          tCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTA‑ttt‑tt‑ttc                                                                                      >  1:4113034/1‑147 (MQ=255)
                                                           cACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTA‑ttt‑tt‑ttct                                                                                     <  2:3785615/149‑4 (MQ=255)
                                                           cACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTA‑ttt‑tt‑ttct                                                                                     <  1:11338550/149‑4 (MQ=255)
                                                              cGCACGAGACACTCAGTATAAAAGTATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGTGTACCCTCCCCAAACACTTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTaa                                                                                   <  2:7906305/149‑1 (MQ=255)
                                                               gCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCTCGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAAt                                                                                  <  1:6946579/149‑1 (MQ=255)
                                                               gCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAAt                                                                                  <  2:1362525/149‑1 (MQ=255)
                                                                 aCGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCANTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTc                                                                                <  1:10206773/149‑1 (MQ=255)
                                                                   gagaCCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCTCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCta                                                                              <  1:6695845/149‑1 (MQ=255)
                                                                   gagaCCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCta                                                                              <  1:1851353/149‑1 (MQ=255)
                                                                    agaCCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCtat                                                                             <  2:7245176/149‑1 (MQ=255)
                                                                    agaCCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCtat                                                                             <  1:10810418/149‑1 (MQ=255)
                                                                      aaccGCAGTCCAAAAGCATGTTGAAGCCAAGCTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCtatta                                                                           <  1:9940478/147‑1 (MQ=255)
                                                                      aCCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTA‑ttt‑tt‑ttc                                                                                      <  1:8333436/137‑3 (MQ=255)
                                                                        ccGAAGTCTAAAAGCATATTGAAGCCAGACTCAGATTGTAAAAGATAATAAGTTGTTCCTGCATCACGAGTAAGGTACCCTCCCCAATCACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATtata                                                                         <  2:578573/149‑1 (MQ=255)
                                                                         cGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAg                                                                        <  1:1469562/149‑1 (MQ=255)
                                                                          gCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGt                                                                       >  2:2514000/1‑149 (MQ=255)
                                                                          gCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGt                                                                       >  1:9559373/1‑149 (MQ=255)
                                                                             gTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCa                                                                    <  2:6105923/149‑1 (MQ=255)
                                                                                 aaaaGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGc                                                                >  1:3131567/1‑149 (MQ=255)
                                                                                     gCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCt                                                            >  1:2017358/1‑149 (MQ=255)
                                                                                        tGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTaa                                                         <  1:7508748/149‑1 (MQ=255)
                                                                                         gTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAAt                                                        <  2:5259493/149‑1 (MQ=255)
                                                                                          ttGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATc                                                       <  1:10297787/149‑1 (MQ=255)
                                                                                           tGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCt                                                      >  1:4795457/1‑149 (MQ=255)
                                                                                           tGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCt                                                      <  1:8052869/149‑1 (MQ=255)
                                                                                            gatgCCAGACTCCGATTTTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTTGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCtt                                                     <  2:1962726/146‑1 (MQ=255)
                                                                                            gAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCtt                                                     <  1:10010327/149‑1 (MQ=255)
                                                                                             aaGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTa                                                    >  1:11165787/1‑149 (MQ=255)
                                                                                                ccAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTg                                                 <  1:3953446/149‑1 (MQ=255)
                                                                                                  aGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTCATTCTATTATAGTTCAAAGCACCTTCATCTTAGTGCt                                               >  2:6761790/1‑149 (MQ=255)
                                                                                                  aGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCt                                               >  2:3097673/1‑149 (MQ=255)
                                                                                                  aGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCt                                               <  1:4548146/149‑1 (MQ=255)
                                                                                                   gACTCCGATTGTAAAAGATAAGAGGCTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTA‑CTTATT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTa                                              >  2:6243346/1‑149 (MQ=255)
                                                                                                       ccGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATATTTTAAAGCACCTTAATCTTATTGCTAGGCg                                          >  2:5440642/1‑149 (MQ=255)
                                                                                                       ccGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACg                                          <  2:7431986/149‑1 (MQ=255)
                                                                                                       ccGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACg                                          <  2:9755782/149‑1 (MQ=255)
                                                                                                        cGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACGt                                         <  1:5543528/149‑1 (MQ=255)
                                                                                                           ttGTAAAAGATAAGAAGTTGTTCCTGCATCCCGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAGTTTTATTATCGTTCAAAGCACCTTAATCTTCGTGCTCTACGTCCt                                      >  2:8337386/1‑149 (MQ=255)
                                                                                                           ttGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACGTCCt                                      >  2:3258930/1‑149 (MQ=255)
                                                                                                           ttGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACGTCCt                                      <  2:2922778/149‑1 (MQ=255)
                                                                                                             gTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACGTCCtat                                    <  2:396488/149‑1 (MQ=255)
                                                                                                              tAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACGTCCtata                                   >  2:2266842/1‑149 (MQ=255)
                                                                                                                aaaGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACGTCCTAtatt                                 >  1:257560/1‑149 (MQ=255)
                                                                                                                 aaGATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATATTTCAAAGCACCTTAATCTTAGTGCTAGACGTCCTAtattt                                >  2:10877408/1‑148 (MQ=255)
                                                                                                                   gATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACGTCCTATattatt                              >  1:9228153/1‑149 (MQ=255)
                                                                                                                   gATAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACGTCCTATattatt                              >  2:6523269/1‑149 (MQ=255)
                                                                                                                     tAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACGTCCTATATTATTCt                            >  1:7099500/1‑149 (MQ=255)
                                                                                                                     tAAGAAGTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACGTCCTATATTATTCt                            <  1:10534059/149‑1 (MQ=255)
                                                                                                                       agaagTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACGTCCTATATTATTCTaa                          <  1:3437595/149‑1 (MQ=255)
                                                                                                                       agaagTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACGTCCTATATTATTCTaa                          <  1:788210/149‑1 (MQ=255)
                                                                                                                       agaagTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACGTCCTATATTATTCTaa                          <  1:5306223/149‑1 (MQ=255)
                                                                                                                         aagTTGTTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACGTCCTATATTATTCTAAAt                        <  2:1841016/149‑1 (MQ=255)
                                                                                                                         aagTTGCTCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCACTGTTAATTTCATTTTTTTCTCTCTTTCTA‑TTT‑TTCTCTTCATTTTATTATATTTCAACGCAGCTTAATCTTTGTTCTAGACGTCATATTTTATTCTAAAt                        >  2:5568916/1‑149 (MQ=255)
                                                                                                                            ttgttCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACGTCCTATATTATTCTAAATGGc                     <  1:2309306/149‑1 (MQ=255)
                                                                                                                            ttgttCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACGTCCTATATTATTCTAAATGGc                     >  1:10666011/1‑149 (MQ=255)
                                                                                                                               ttCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACGTCCTATATTATTCTAAATGGCACt                  >  1:4367152/1‑149 (MQ=255)
                                                                                                                                tCCTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACGTCCTATATTATTCTAAATGGCACtt                 <  2:11242190/149‑1 (MQ=255)
                                                                                                                                 ccTGCATCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTATTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TTTTAATTCTATTATAGTTCAAAGCACCTTCATCTTAGTGTTAGACGTCCTATATTATTCTAAATGGCAtttt                >  2:4100198/1‑148 (MQ=255)
                                                                                                                                      aTCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTTTTAATTTCATTTTTTTCGCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTAAATCTTAGTGCTAGACGTCCTATATTATTCTAAACGGCACTTATGCAc           <  2:5005992/149‑1 (MQ=255)
                                                                                                                                       tCACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACGTCCTATATTATTCTAAATGGCACTTATCCACt          >  1:5023477/1‑149 (MQ=255)
                                                                                                                                        cACGAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTGATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACGTCCTATGTTATTCTAAATGGCACTTATCCACtt         >  1:7307652/1‑149 (MQ=255)
                                                                                                                                           gAGTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACGTCCTATATTATTCTAAATGGCACTTATCCACTTCaa      >  1:7712450/1‑149 (MQ=255)
                                                                                                                                             gTGGGGTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTATCTCTCTATCTATTTT‑TT‑TCTTAATTCTAGTATAGTTCAAACAACCTTAATCTTAGTGCTAGACGTCCTATATTATTCTAAATGGCACTTATCCACTTCAAtt    >  2:8221745/1‑149 (MQ=255)
                                                                                                                                               ggggTACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTATTTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACGTCCTATATTATTCTAAATGGCACTTATCCACTTCAATtat  <  2:4342638/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TAATGGCTCAAAATAACCGCATCCAACTGAGTCGGTTGTAACTCATGTTCAAGCTCAGTCACCGCACGAGACCCGCAGTCTAAAAGCATGTTGAAGCCAGACTCCGATTGTAAAAGATAAGAAGTTGTTCCTGCATCACGAGTGGGATACCCTCCCCAAACACCTAGCGCTGTTAATTTCATTTTTTTCTCTCTTTCTA‑TTT‑TT‑TCTTAATTCTATTATAGTTCAAAGCACCTTAATCTTAGTGCTAGACGTCCTATATTATTCTAAATGGCACTTATCCACTTCAATTAT  >  AM406671/1826261‑1826551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑