BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,854,998	T→A	V95V (GTA→GTT)	glgA ←	GlgA protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,854,998	0	T	A	99.9%	5229.6 / NA	1329	V95V (GTA→GTT)	glgA	GlgA protein
Reads supporting (aligned to +/- strand): ref base T (1/0); major base A (624/701); minor base C (2/0); total (628/701)
Fisher's exact test for biased strand distribution p-value = 2.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1333 total aligned reads displayed.

ACATGCAAAATGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTTACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCAACAGAAG  >  AM406671/1854852‑1855143

aCATGCAAAATGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCGCATTATCTAAAAAATAATATTTAAc                                                                                                                                                 <  2:6283400/149‑1 (MQ=255)

aCATGCAAAATGTCAGCAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAAc                                                                                                                                                 <  1:7685327/149‑1 (MQ=255)

 cATGCAAAATATCAGGAATAAAATCTAATTTTTCTAAGAGCATACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACa                                                                                                                                                <  1:5198977/149‑1 (MQ=255)

   tGCAAAATGTCAGGAATAAAATCTAATTTTTATAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAAGCGTAAATCTGCCCTCCATCAAAATGATGCTCATTAAAAAAAAAATAATATTTAACAtt                                                                                                                                              <  2:5495469/149‑1 (MQ=255)

     cAAAATGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATAGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTAt                                                                                                                                            <  1:4556975/149‑1 (MQ=255)

     aaaaaTGTCAGGAATAAAACCTAATTTTTCTAAGGGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTAt                                                                                                                                            <  2:1913148/148‑1 (MQ=255)

        aaTGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCtt                                                                                                                                         >  2:10207933/1‑149 (MQ=255)

         aTGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCttt                                                                                                                                        <  2:6566376/149‑1 (MQ=255)

         aTGTCAGGAATAAAATCTAATGTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTACTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTAGCTAAAAAATAATATTTAACATTATCttt                                                                                                                                        <  2:5961742/149‑1 (MQ=255)

          tGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCtttt                                                                                                                                       >  2:2451380/1‑149 (MQ=255)

           gTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTGGCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTa                                                                                                                                      >  2:7245040/1‑149 (MQ=255)

            tCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTAc                                                                                                                                     >  1:9109315/1‑149 (MQ=255)

             cAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTGCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACt                                                                                                                                    <  2:79838/149‑1 (MQ=255)

              aGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTa                                                                                                                                   >  2:10022611/1‑149 (MQ=255)

               ggAATAAAATCTAATTTTTCTAAGAGATGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTaa                                                                                                                                  <  2:174707/149‑1 (MQ=255)

                 aaTAAAATCTAATTTTTTTTTTTTTTTACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTTCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTATATAATAATATTTAACATTATCTTTTACTAAgg                                                                                                                                >  2:10847748/1‑149 (MQ=255)

                 aaTAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAgg                                                                                                                                >  2:2944173/1‑149 (MQ=255)

                 aaTAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAgg                                                                                                                                <  2:10592707/149‑1 (MQ=255)

                   tAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGtt                                                                                                                              >  2:10995898/1‑149 (MQ=255)

                     aaaTCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGtttt                                                                                                                            <  2:8157809/149‑1 (MQ=255)

                     aaaTCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGtttt                                                                                                                            >  2:2206028/1‑149 (MQ=255)

                      aaTCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTa                                                                                                                           >  1:5766519/1‑149 (MQ=255)

                       aTCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTaa                                                                                                                          <  1:11109002/149‑1 (MQ=255)

                       aTCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTaa                                                                                                                          >  2:4272150/1‑149 (MQ=255)

                        tCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAAc                                                                                                                         >  2:4022093/1‑149 (MQ=255)

                         cTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACt                                                                                                                        <  1:7901924/149‑1 (MQ=255)

                         cTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACt                                                                                                                        <  1:1564033/149‑1 (MQ=255)

                                ttCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAAGATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACat                                                                                                                 >  1:8213207/1‑149 (MQ=255)

                                     agagCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCa                                                                                                            <  2:749047/149‑1 (MQ=255)

                                     agagCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCa                                                                                                            <  2:3165132/149‑1 (MQ=255)

                                      gagCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCAt                                                                                                           <  2:9504642/149‑1 (MQ=255)

                                      gagCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCAt                                                                                                           >  1:378741/1‑149 (MQ=255)

                                       agCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATg                                                                                                          >  1:2626031/1‑149 (MQ=255)

                                        gCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATgc                                                                                                         <  1:4945293/149‑1 (MQ=255)

                                         cTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAGATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATgcc                                                                                                        >  1:6952596/1‑149 (MQ=255)

                                          tGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATgccg                                                                                                       >  1:4176433/1‑149 (MQ=255)

                                          tGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATgccg                                                                                                       >  1:9771121/1‑149 (MQ=255)

                                            aCAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATgccgcc                                                                                                     >  1:3435284/1‑149 (MQ=255)

                                             cAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCa                                                                                                    <  2:6112911/149‑1 (MQ=255)

                                               aaCGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAAc                                                                                                  >  2:11332491/1‑149 (MQ=255)

                                                 cGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCt                                                                                                <  1:2231133/149‑1 (MQ=255)

                                                     tAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACAt                                                                                            >  1:8473277/1‑149 (MQ=255)

                                                     tAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACAt                                                                                            <  1:3930055/149‑1 (MQ=255)

                                                     tAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACAt                                                                                            <  1:9283566/149‑1 (MQ=255)

                                                             aaaaGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACaaaa                                                                                    >  1:6184238/1‑149 (MQ=255)

                                                                 ggCAAAACGAGCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCAt                                                                                >  2:10436776/1‑149 (MQ=255)

                                                                  gCAAAACGCTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCCAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATCTTCATGCCGCCAACCTACATCAACAAAATCATa                                                                               >  2:5828311/1‑149 (MQ=255)

                                                                      aaCGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAAt                                                                           <  2:2051803/149‑1 (MQ=255)

                                                                        cGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTc                                                                         <  1:8089510/149‑1 (MQ=255)

                                                                         gTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCa                                                                        <  1:1718062/149‑1 (MQ=255)

                                                                            ctcCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATcg                                                                     <  1:891359/149‑1 (MQ=255)

                                                                             tcCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATcga                                                                    >  2:6911545/1‑149 (MQ=255)

                                                                              ccATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATcgac                                                                   >  2:5084368/1‑149 (MQ=255)

                                                                               catcatCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATcgacg                                                                  >  1:3253811/1‑149 (MQ=255)

                                                                                 tcatcaCCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGag                                                                <  2:153224/149‑1 (MQ=255)

                                                                                  catcaCCATAACCGTAAATCTGCCCTCGACCAACATAATGCTCATTATCTACAAACTAATATTTAACCTTCTCTTTTACTCAGGGTCTACCTCCAACATATCCATCCCGCCAACCTCCCTCACCACCATCATACAATTCATCGCCGcgt                                                               >  2:2308096/1‑149 (MQ=255)

                                                                                  catcaCCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGagt                                                               <  1:10024164/149‑1 (MQ=255)

                                                                                    tcaCCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGagtag                                                             >  1:1829787/1‑149 (MQ=255)

                                                                                    tcaCCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGagtag                                                             <  1:10363636/149‑1 (MQ=255)

                                                                                     caCCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGagtagt                                                            <  2:8493418/149‑1 (MQ=255)

                                                                                            aCCGTAAATCTGCCCTCGACCAGAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGat                                                     <  2:7873462/149‑1 (MQ=255)

                                                                                            aCCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGat                                                     <  2:8948709/149‑1 (MQ=255)

                                                                                              cGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGatat                                                   <  2:8386939/149‑1 (MQ=255)

                                                                                                tAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATAttc                                                 >  1:10674132/1‑149 (MQ=255)

                                                                                                 aaaTCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATAttct                                                <  1:2959101/149‑1 (MQ=255)

                                                                                                 aaaTCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATAttct                                                <  1:7503527/149‑1 (MQ=255)

                                                                                                 aaaTCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATAttct                                                >  1:722338/1‑149 (MQ=255)

                                                                                                    tCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATtcttct                                             <  1:9507078/149‑1 (MQ=255)

                                                                                                    tCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTATAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATtcttct                                             >  2:4456935/1‑149 (MQ=255)

                                                                                                            cGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCtttcatttc                                     <  1:10160810/149‑1 (MQ=255)

                                                                                                                 aaaaTAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTTCATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCAtttt                                >  2:3859747/1‑149 (MQ=255)

                                                                                                                 aaaaTAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCAtttt                                <  1:10517483/149‑1 (MQ=255)

                                                                                                                  aaaTAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCAttttt                               <  1:55357/149‑1 (MQ=255)

                                                                                                                   aataatGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACATCATCATAAAATTCATCGACGAGTAGTGAACGCTATTCTTCTTTCATTTCATTTTTg                              <  2:9307088/149‑1 (MQ=255)

                                                                                                                   aataatGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTg                              >  2:8688626/1‑149 (MQ=255)

                                                                                                                     taatGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGaa                            <  1:4362461/149‑1 (MQ=255)

                                                                                                                     taatGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGaa                            <  2:3632861/149‑1 (MQ=255)

                                                                                                                        tGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATa                         >  2:1346129/1‑149 (MQ=255)

                                                                                                                          cTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAg                       >  2:9138907/1‑149 (MQ=255)

                                                                                                                            cATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATCTTCATGCCGCCAACCTACCTCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCCTTTCATTTTTGAAATAACGt                     >  2:3326128/1‑149 (MQ=255)

                                                                                                                            cATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGt                     >  1:798689/1‑149 (MQ=255)

                                                                                                                            cATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGt                     >  1:6000292/1‑149 (MQ=255)

                                                                                                                             aTTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTa                    <  1:6395090/149‑1 (MQ=255)

                                                                                                                             aTTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTa                    <  1:9889766/149‑1 (MQ=255)

                                                                                                                             aTTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTa                    >  1:10810985/1‑149 (MQ=255)

                                                                                                                              ttATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTaa                   >  2:7122960/1‑149 (MQ=255)

                                                                                                                               tATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTaaa                  >  1:1323293/1‑149 (MQ=255)

                                                                                                                                 tCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAAt                <  1:4785128/149‑1 (MQ=255)

                                                                                                                                   tAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGATAAAATca              >  1:6577006/1‑149 (MQ=255)

                                                                                                                                    aaaaaaTAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATcac             >  1:11326973/1‑149 (MQ=255)

                                                                                                                                    aaaaaaTAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATcac             >  1:6703402/1‑149 (MQ=255)

                                                                                                                                     aaaaaTAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATcaca            <  2:4695182/149‑1 (MQ=255)

                                                                                                                                      aaaaTAATCTTTAACATTATCTTTTACTAAGGTACTAACTCCAACATACTCATGCCGCTAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCacac           >  2:2681645/1‑149 (MQ=255)

                                                                                                                                      aaaaTAATATGTAAATTTATCTTTTACTAAGGTTCTAACTCCAACATATTCATGCAGCCAACTTACATCAACAAAATGATAAATTTCATGGACGATTATTGAACGATATTCTTCTTTCATATCATTTTTGAAATAAGGTAGCATCacac           >  2:7715223/1‑149 (MQ=255)

                                                                                                                                       aaaTAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACAcc          <  1:8658625/149‑1 (MQ=255)

                                                                                                                                        aataatATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCa         >  2:5256127/1‑149 (MQ=255)

                                                                                                                                           aataTTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCAACa      >  1:5422216/1‑149 (MQ=255)

                                                                                                                                              atTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCAACagaa   >  2:4955318/1‑149 (MQ=255)

                                                                                                                                              atTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCAACagaa   >  1:2425145/1‑149 (MQ=255)

                                                                                                                                               tttaACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCAACagaag  <  2:6457053/149‑1 (MQ=255)

ACATGCAAAATGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTTACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCAACAGAAG  >  AM406671/1854852‑1855143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`