BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	2,266,724	T→C	I10T (ATT→ACT)	argR →	arginine repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	2,266,724	0	T	C	99.9%	5856.7 / NA	1434	I10T (ATT→ACT)	argR	arginine repressor
Reads supporting (aligned to +/- strand): ref base T (0/1); major base C (741/690); minor base G (1/1); total (742/692)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1453 total aligned reads displayed.

ACCATCGATTGCAGCAGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTATTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACGGGACAAAGGAGAG  >  AM406671/2266578‑2266869

  cATCGATTGCAGCAGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTaaa                                                                                                                                               >  1:2035921/1‑149 (MQ=255)

   tttgATTGCAACAGACCATGCTTGGCTTATTAGTTATTTTTCATCCATTCATTAATTATACAAAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAg                                                                                                                                              <  2:10078658/146‑1 (MQ=255)

    tCGATTGCAGCAGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGa                                                                                                                                             >  1:10884176/1‑149 (MQ=255)

    tCGATTGCAGCAGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGa                                                                                                                                             >  1:6079402/1‑149 (MQ=255)

          gcagcaGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATtgt                                                                                                                                       <  1:278426/149‑1 (MQ=255)

             gcagACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGAc                                                                                                                                    >  1:8774175/1‑149 (MQ=255)

              cagACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACa                                                                                                                                   <  1:7069065/149‑1 (MQ=255)

               agACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACaa                                                                                                                                  >  1:2554954/1‑149 (MQ=255)

                  cAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTAGGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAaata                                                                                                                               <  2:3342877/149‑1 (MQ=255)

                   aGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAaataa                                                                                                                              >  2:1932973/1‑149 (MQ=255)

                     tGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAataata                                                                                                                            <  2:2576024/149‑1 (MQ=255)

                       cTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATaaa                                                                                                                          >  2:4723102/1‑149 (MQ=255)

                        ttGGCTTACTAGTTGTTTTTCTTCTATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATaaaa                                                                                                                         >  2:11263701/1‑149 (MQ=255)

                        ttGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATaaaa                                                                                                                         <  2:5795463/149‑1 (MQ=255)

                             ttACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTg                                                                                                                    >  1:7776601/1‑149 (MQ=255)

                              tACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGa                                                                                                                   >  1:8314582/1‑149 (MQ=255)

                                 tAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTc                                                                                                                >  1:10400838/1‑149 (MQ=255)

                                    ttgttTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCaag                                                                                                             <  2:8735500/149‑1 (MQ=255)

                                          ttCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGc                                                                                                       <  1:7305041/149‑1 (MQ=255)

                                           tCGTGCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAATGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCt                                                                                                      >  2:822603/1‑149 (MQ=255)

                                            cGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCtt                                                                                                     >  1:9715438/1‑149 (MQ=255)

                                              tCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCa                                                                                                   >  1:47462/1‑149 (MQ=255)

                                              tCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCa                                                                                                   <  1:1886509/149‑1 (MQ=255)

                                              tCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCa                                                                                                   >  2:1384829/1‑149 (MQ=255)

                                               ccATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCaa                                                                                                  >  1:10006158/1‑149 (MQ=255)

                                               ccATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCaa                                                                                                  <  2:9636704/149‑1 (MQ=255)

                                               ccATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCaa                                                                                                  >  1:6671115/1‑149 (MQ=255)

                                                 aTTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAAtc                                                                                                >  2:5377257/1‑149 (MQ=255)

                                                   tCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAAtctc                                                                                              <  2:229352/149‑1 (MQ=255)

                                                   tCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAAtctc                                                                                              >  2:7977293/1‑149 (MQ=255)

                                                   tCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGATTCAAtctc                                                                                              >  2:2735620/1‑149 (MQ=255)

                                                    cACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATctct                                                                                             >  1:11101714/1‑149 (MQ=255)

                                                    cACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATctct                                                                                             <  1:5243559/149‑1 (MQ=255)

                                                      cTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCtat                                                                                           >  2:11010309/1‑149 (MQ=255)

                                                            ataCAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAg                                                                                     <  1:2436756/149‑1 (MQ=255)

                                                            ataCAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAg                                                                                     >  2:6115695/1‑149 (MQ=255)

                                                             taCAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATTCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGa                                                                                    >  2:1740491/1‑149 (MQ=255)

                                                             taCAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGa                                                                                    <  1:3732686/149‑1 (MQ=255)

                                                              aCAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGaa                                                                                   >  1:3355429/1‑149 (MQ=255)

                                                               cAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAAc                                                                                  >  2:3803153/1‑149 (MQ=255)

                                                               cAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAAc                                                                                  <  2:3153417/149‑1 (MQ=255)

                                                                 gAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAAcgc                                                                                >  1:8469672/1‑149 (MQ=255)

                                                                  aaaaaaCACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAATAAGAGCTTCAATCTCTATTATTAGAAcgcg                                                                               >  1:3541041/1‑149 (MQ=255)

                                                                  aaaaaaCACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAAcgcg                                                                               <  1:1323802/149‑1 (MQ=255)

                                                                    aaaaCACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGgt                                                                             >  1:9145457/1‑149 (MQ=255)

                                                                         acTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTaga                                                                        >  1:7642647/1‑149 (MQ=255)

                                                                          cTAATTTTGTCAATATATAGACCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTagag                                                                       <  1:927485/149‑1 (MQ=255)

                                                                           tAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAgg                                                                      >  2:358534/1‑149 (MQ=255)

                                                                            aaTTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGAGAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCACTCTCTATTATTAGAACGCGGTGTAGAggg                                                                     >  2:6696864/1‑148 (MQ=255)

                                                                                 tGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAacac                                                                <  2:4235196/149‑1 (MQ=255)

                                                                                 tGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAacac                                                                <  2:10804883/149‑1 (MQ=255)

                                                                                  gTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAAcaca                                                               <  2:9181100/149‑1 (MQ=255)

                                                                                   tCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACaa                                                              >  2:7117795/1‑149 (MQ=255)

                                                                                     aaTATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGc                                                            >  1:10639937/1‑149 (MQ=255)

                                                                                      atatatAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGcc                                                           >  2:6527270/1‑149 (MQ=255)

                                                                                       tatataGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCa                                                          >  2:4900416/1‑149 (MQ=255)

                                                                                       tatataGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGAAACAAGAAGAGATTAGATGTCTATTAATAGAAAGCGGAATAGAGATAACACAAGACa                                                          >  2:7773684/1‑149 (MQ=255)

                                                                                         tataGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACt                                                        >  1:3202001/1‑149 (MQ=255)

                                                                                          ataGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACtt                                                       <  1:6807750/149‑1 (MQ=255)

                                                                                           taGTCCCTTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATTTCTATTATTAGAACGCTGTGTAGATGTAACACAAGCCACttt                                                      >  2:1195319/1‑149 (MQ=255)

                                                                                            aGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTa                                                     <  2:9383129/149‑1 (MQ=255)

                                                                                               ccATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATcc                                                  <  2:10516710/149‑1 (MQ=255)

                                                                                                cATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCa                                                 <  1:6545065/149‑1 (MQ=255)

                                                                                                  ttATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCaga                                               <  1:4512995/149‑1 (MQ=255)

                                                                                                  ttATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCaga                                               <  1:812784/149‑1 (MQ=255)

                                                                                                  ttATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCaga                                               >  1:6206466/1‑149 (MQ=255)

                                                                                                   tataTGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCagag                                              <  1:9475522/149‑1 (MQ=255)

                                                                                                   tataTGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACGCAAGAAGAGCTGCAATCTCTATTATTAGAACGCGGTGGAGAGGTAACACAAGCCACTTTATCCagag                                              <  2:952584/149‑1 (MQ=255)

                                                                                                    atatGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATAATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAgaga                                             >  2:4528733/1‑149 (MQ=255)

                                                                                                      atGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGata                                           <  2:8528521/149‑1 (MQ=255)

                                                                                                       tgctataATTATGCTATGAAAAGAGATAAAAGATTAGTAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACCCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGatat                                          <  2:6385774/146‑1 (MQ=255)

                                                                                                       tGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGatat                                          >  2:7617366/1‑149 (MQ=255)

                                                                                                        ggTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATAtt                                         <  1:8591182/149‑1 (MQ=255)

                                                                                                          tataATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCATAGATATTAg                                       <  2:3455956/149‑1 (MQ=255)

                                                                                                          tataATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAg                                       >  1:7931907/1‑149 (MQ=255)

                                                                                                           ataATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGa                                      <  2:10232326/149‑1 (MQ=255)

                                                                                                             aaTTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGTGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGaaa                                    <  1:5892941/149‑1 (MQ=255)

                                                                                                             aaTTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGATTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACAACAGCCACTTTATACAGAGATATTAGaaa                                    >  2:1544747/1‑149 (MQ=255)

                                                                                                              aTTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGaaaa                                   <  1:7479788/149‑1 (MQ=255)

                                                                                                                tATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAAtt                                 >  1:4876268/1‑149 (MQ=255)

                                                                                                                  tGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTaa                               >  1:2209145/1‑149 (MQ=255)

                                                                                                                    cTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAat                             >  2:8985509/1‑149 (MQ=255)

                                                                                                                     tATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAata                            >  1:2989341/1‑149 (MQ=255)

                                                                                                                       tGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAAtatt                          >  2:5585088/1‑149 (MQ=255)

                                                                                                                        gAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAAtatta                         <  2:5961921/149‑1 (MQ=255)

                                                                                                                            agagATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATaaa                     >  1:502843/1‑149 (MQ=255)

                                                                                                                            agagATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTCACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGTGATATTCGAAAATTAAATATTATaaa                     >  1:4089726/1‑149 (MQ=255)

                                                                                                                                 tAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAAc                <  1:11319978/149‑1 (MQ=255)

                                                                                                                                    aaGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACggg             >  1:1627674/1‑149 (MQ=255)

                                                                                                                                     aGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCGAGAGATATTAGAAAATTAAATATTATAAAAAAACGGGa            >  2:9815888/1‑149 (MQ=255)

                                                                                                                                     aGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATAGTCGAAAATTAAATATTATAAAAAAACGGGa            >  2:5107063/1‑149 (MQ=255)

                                                                                                                                      gATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAACTATTATAAAAAAACGGGAc           >  1:5623262/1‑149 (MQ=255)

                                                                                                                                      gATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACGGGAc           >  1:5423636/1‑149 (MQ=255)

                                                                                                                                         tAGAAATTACTAAAGGAATTGTGACAAATAATAAGATGTTGACTCAAGAAGAGCTTCAAGCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGGGATATTAGAAAATTAAATATTATAAAAAAACGGGACaaa        <  2:2466730/149‑1 (MQ=255)

                                                                                                                                         tAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACGGGACaaa        >  2:683415/1‑149 (MQ=255)

                                                                                                                                          aGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACGGGACAAAg       >  2:2136475/1‑149 (MQ=255)

                                                                                                                                           aaaaTTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACGGGACAAAgg      <  1:4346029/148‑1 (MQ=255)

                                                                                                                                           aaaaTTACTAAAGAAATGGTGACAAATAATAAAATAAAGACTCAAGAAGAACTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACAAAAACCACTTTATCCAGAGATATTAGAAAATGAAATATTATAAAAAAACGGGACAAAgg      <  2:7345413/148‑1 (MQ=255)

                                                                                                                                             aaTTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACGGGACAAAGgag    >  1:893255/1‑149 (MQ=255)

                                                                                                                                              attactAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACGGGACAAAGgaga   >  1:9895043/1‑149 (MQ=255)

ACCATCGATTGCAGCAGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTATTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACGGGACAAAGGAGAG  >  AM406671/2266578‑2266869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`