BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,588,245	2 bp→AT	coding (837‑838/1170 nt)	ugd ←	UDP‑glucose 6‑dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,588,245	0	T	A	99.7%	3729.9 / NA	992	T280S (ACA→TCA)	ugd	UDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (1/0); major base A (516/470); minor base C (1/2); total (518/474)
Fisher's exact test for biased strand distribution p-value = 6.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	AM406671	1,588,246	0	A	T	99.4%	3785.0 / NA	992	N279K (AAT→AAA)	ugd	UDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand): ref base A (0/2); major base T (515/467); minor base G (2/4); total (518/474)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 994 total aligned reads displayed.

TTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTTGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGTATTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATTATGTGTCCCAATCCGTGAATCCAACCCAACAC  >  AM406671/1588102‑1588388

||

ttGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGtttgatttga                                                                                                                                            <  2:11104274/149‑1 (MQ=255)

   cAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAACTTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACaa                                                                                                                                         <  1:8280385/149‑1 (MQ=255)

      gagTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACATTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAg                                                                                                                                      <  1:5866839/149‑1 (MQ=255)

      gagTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGATTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGGTTCGTTTGATTTGACAATaa                                                                                                                                      >  1:1147363/1‑148 (MQ=255)

       agTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGc                                                                                                                                     >  2:10792370/1‑149 (MQ=255)

          ttCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACTGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGt                                                                                                                                  >  1:1323267/1‑149 (MQ=255)

           tcatcaCATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGtc                                                                                                                                 >  1:11182517/1‑149 (MQ=255)

            catcacatcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGtca                                                                                                                                <  1:10348337/149‑1 (MQ=255)

             atcacatcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGtcat                                                                                                                               <  1:5357390/149‑1 (MQ=255)

               cacatcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGtcatca                                                                                                                             <  1:9887956/149‑1 (MQ=255)

                acatcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCaa                                                                                                                            <  1:9791379/149‑1 (MQ=255)

                 catcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAAc                                                                                                                           <  1:87151/149‑1 (MQ=255)

                 catcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAAc                                                                                                                           >  2:7530018/1‑149 (MQ=255)

                  atcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACt                                                                                                                          <  2:711919/149‑1 (MQ=255)

                       taatCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGa                                                                                                                     <  2:10546780/149‑1 (MQ=255)

                         atCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAgg                                                                                                                   >  2:7376764/1‑149 (MQ=255)

                         atCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAgg                                                                                                                   >  1:6764774/1‑149 (MQ=255)

                              aCTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGc                                                                                                              >  2:2073612/1‑149 (MQ=255)

                              aCTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGc                                                                                                              >  1:9021012/1‑149 (MQ=255)

                                 ctgtctgAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCt                                                                                                           <  2:8487866/149‑1 (MQ=255)

                                   gtctgAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCa                                                                                                         <  1:4495088/149‑1 (MQ=255)

                                   gtctgAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGCTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCa                                                                                                         >  1:10579535/1‑149 (MQ=255)

                                    tctgAACTTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACCGGCTGTGGAGATTTAGCCATAAACTGATCAACAATGTGCTGCTTTCAGGGTTCGTGTGATTTGACAATAGCCGTACTCAACTCCTGAGGCACGCCCTCaa                                                                                                        >  2:1454604/1‑148 (MQ=255)

                                    tatgACATTATCTGACCCAGCTTTCATGACCAACCGGTAACTTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGGTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCAt                                                                                                        <  2:8249345/147‑1 (MQ=255)

                                       gAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGTTGTTTTTGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAAc                                                                                                     >  2:6439869/1‑149 (MQ=255)

                                        aaaTTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACt                                                                                                    >  2:863122/1‑149 (MQ=255)

                                         aaTTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTg                                                                                                   >  1:7613526/1‑149 (MQ=255)

                                          aTTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGATTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTgg                                                                                                  <  2:7668425/149‑1 (MQ=255)

                                           ttATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTgtg                                                                                                 >  1:2262759/1‑147 (MQ=255)

                                                 gACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAg                                                                                           <  1:4954609/149‑1 (MQ=255)

                                                   cccAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAgct                                                                                         <  1:9203799/149‑1 (MQ=255)

                                                    ccAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAgctg                                                                                        >  1:3720131/1‑149 (MQ=255)

                                                     cAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGtcat                                                                                                                               >  2:6028811/1‑109 (MQ=255)

                                                        cTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCctt                                                                                    >  1:2528376/1‑149 (MQ=255)

                                                         tttCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTg                                                                                   <  2:6900805/149‑1 (MQ=255)

                                                            cATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGt                                                                                <  1:2920991/149‑1 (MQ=255)

                                                               gACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCtt                                                                             <  2:4572529/149‑1 (MQ=255)

                                                               gACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCtt                                                                             >  1:4722551/1‑149 (MQ=255)

                                                                 ccAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTc                                                                           >  2:2856274/1‑149 (MQ=255)

                                                                  cAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCg                                                                          >  1:1858205/1‑149 (MQ=255)

                                                                   aaCCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCgg                                                                         <  1:2402577/149‑1 (MQ=255)

                                                                     ccGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAAAAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAAGAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGATGCTTTGAGTCTTTCGGCa                                                                       >  2:5547403/1‑149 (MQ=255)

                                                                       ggTAAATTCCCACAGTTTTTGGTGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGCGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCTGCaaa                                                                     <  2:663467/149‑1 (MQ=255)

                                                                         tAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACa                                                                   >  1:8578972/1‑149 (MQ=255)

                                                                          aaaTTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACaa                                                                  >  1:10000247/1‑149 (MQ=255)

                                                                          aaaTTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACaa                                                                  <  1:9590516/149‑1 (MQ=255)

                                                                               ccAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATGGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAacc                                                             <  1:6976258/149‑1 (MQ=255)

                                                                               ccAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAacc                                                             >  2:10208991/1‑149 (MQ=255)

                                                                                  aCAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAaccacc                                                          <  1:8895214/149‑1 (MQ=255)

                                                                                    aGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCAt                                                        <  2:8131062/149‑1 (MQ=255)

                                                                                     gTTTTTGGAGATTTAGCCATAATCGTCTCAACAATGTGCAGTTTTCTCGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGACGAACGCCCTCATAACTGGCCACAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATa                                                       <  2:9528331/149‑1 (MQ=255)

                                                                                       ttttgGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAAc                                                     <  2:4262482/149‑1 (MQ=255)

                                                                                       ttttgGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAAc                                                     <  2:3055520/149‑1 (MQ=255)

                                                                                          tggagaTTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCaa                                                  <  1:7941178/147‑1 (MQ=255)

                                                                                             agaTTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAAc                                               <  1:638517/149‑1 (MQ=255)

                                                                                             agaTTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAAc                                               >  2:25430/1‑149 (MQ=255)

                                                                                              gaTTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACt                                              >  1:6044784/1‑149 (MQ=255)

                                                                                              gaTTTAGCCACAATCTGCTCAACACTGTGCTGTTTTCTGGTTTCGTTTCATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGTCCAAACGCTGCTTTGAGTCTTTCGGCATACAATAACCACCATAACCAAACCt                                              <  2:390407/149‑1 (MQ=255)

                                                                                               aTTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACtt                                             <  1:1103503/149‑1 (MQ=255)

                                                                                                tttAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTg                                            <  2:1289621/149‑1 (MQ=255)

                                                                                                 ttAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTgg                                           <  1:8395399/149‑1 (MQ=255)

                                                                                                  tAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGa                                          <  1:3527750/149‑1 (MQ=255)

                                                                                                   aGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGat                                         >  2:8864403/1‑149 (MQ=255)

                                                                                                     ccATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGatta                                       >  1:7507902/1‑149 (MQ=255)

                                                                                                      cATAATCTGCTCAACAATGTGCTGTCTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCGTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGattat                                      >  2:6257711/1‑149 (MQ=255)

                                                                                                       aTAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTTACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGattatt                                     <  1:10864674/149‑1 (MQ=255)

                                                                                                       aTAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGattatt                                     >  1:5172850/1‑149 (MQ=255)

                                                                                                        tAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGattatta                                    >  2:5829554/1‑149 (MQ=255)

                                                                                                         aaTCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGattattat                                   <  1:1580058/149‑1 (MQ=255)

                                                                                                           tCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGattattataa                                 >  2:9049392/1‑147 (MQ=255)

                                                                                                           tCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGattattataa                                 <  2:6776466/149‑3 (MQ=255)

                                                                                                           tCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGattattataa                                 <  1:9386614/149‑3 (MQ=255)

                                                                                                            cTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTTGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAATACTTGGATtattataat                                <  2:7933021/149‑1 (MQ=255)

                                                                                                             tGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAAtg                               <  2:5171237/149‑1 (MQ=255)

                                                                                                              gCTCAACAATGTGCTGTTTGCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAAtgt                              <  1:5608833/149‑1 (MQ=255)

                                                                                                               cTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAAtgtg                             <  2:11182517/149‑1 (MQ=255)

                                                                                                                tCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATgtgt                            >  2:9457704/1‑149 (MQ=255)

                                                                                                                  aacaaTGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTcc                          >  2:1655714/1‑149 (MQ=255)

                                                                                                                  aacaaTGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTcc                          >  2:336635/1‑149 (MQ=255)

                                                                                                                   acaaTGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTccc                         >  2:1007753/1‑149 (MQ=255)

                                                                                                                      aTGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAAt                      <  1:7177683/149‑1 (MQ=255)

                                                                                                                      aTGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAAt                      >  2:3657511/1‑149 (MQ=255)

                                                                                                                      aTGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAAt                      >  1:4063408/1‑149 (MQ=255)

                                                                                                                       tgtgCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGGACGCTCTCATAACTGGCCAAAAGCTGCTTGGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATc                     >  2:11381647/1‑149 (MQ=255)

                                                                                                                         tgCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCg                   >  2:9707303/1‑149 (MQ=255)

                                                                                                                          gCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGt                  >  1:7108415/1‑149 (MQ=255)

                                                                                                                           cTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTg                 >  2:9117385/1‑149 (MQ=255)

                                                                                                                           cTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCCTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACATGGATTATAATAATGTGTCACAATCCGTg                 >  2:5352182/1‑149 (MQ=255)

                                                                                                                            tGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGa                <  2:10866928/149‑1 (MQ=255)

                                                                                                                            tGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGa                <  1:8171201/149‑1 (MQ=255)

                                                                                                                             gTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGaa               >  1:3168476/1‑149 (MQ=255)

                                                                                                                                   tGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATccaac         >  1:271279/1‑149 (MQ=255)

                                                                                                                                    ggTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATccaacc        >  1:1019432/1‑149 (MQ=255)

                                                                                                                                     gTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATccaaccc       >  2:2329713/1‑149 (MQ=255)

                                                                                                                                      tttCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATccaaccca      >  2:4912231/1‑149 (MQ=255)

                                                                                                                                      tttCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAAACAAAACTTGGATTATTATAATGTGTCCCAATACGTGAATccaaccca      >  2:3514467/1‑149 (MQ=255)

                                                                                                                                       ttCGTTTTATTTAACAATAGACGTCATCAACTCATGAGTAACGCCCTCATAACTGGCCAAAAGCTGCTTTGATTCTTTCGTCAAACAATAACCACCATAACCAAAACTTGCATTATTATAATGTGTCCCAATCCTTGAATccaacccaa     <  2:4077127/149‑1 (MQ=255)

                                                                                                                                        tCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATccaacccaac    >  1:6572974/1‑149 (MQ=255)

                                                                                                                                        tCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATccaacccaac    <  1:10681353/149‑1 (MQ=255)

                                                                                                                                         cGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAaca   <  2:10307479/149‑1 (MQ=255)

||

TTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTTGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGTATTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATTATGTGTCCCAATCCGTGAATCCAACCCAACAC  >  AM406671/1588102‑1588388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`