Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 1,933,214 2 bp→CG coding (224‑225/714 nt) atpB ← ATP synthase F0, A subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM4066711,933,2140GC99.7% 4522.7 / NA 1085G75A (GGC→GCG) atpBATP synthase F0, A subunit
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base C (540/541);  total (543/542)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*AM4066711,933,2150CG99.7% 4499.2 / NA 1085G75A (GGC→GCG) atpBATP synthase F0, A subunit
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base G (543/537);  total (543/542)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1085 total aligned reads displayed.

ACAAGAAGTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGGCCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGAAAACAAT  >  AM406671/1933071‑1933357
                                                                                                                                               ||                                                                                                                                              
   agaagTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCg                                                                                                                                         >  1:10272590/1‑149 (MQ=255)
     aagTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCgtg                                                                                                                                       <  2:5405157/149‑1 (MQ=255)
       gTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCgtggg                                                                                                                                     >  2:3998637/1‑148 (MQ=255)
        tGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCgtggtg                                                                                                                                    >  1:10547439/1‑149 (MQ=255)
          cAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCt                                                                                                                                  >  1:880516/1‑149 (MQ=255)
            aGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTc                                                                                                                                <  1:7416987/149‑1 (MQ=255)
            aGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTc                                                                                                                                >  1:7587740/1‑149 (MQ=255)
             gtgtCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCa                                                                                                                               >  1:5511049/1‑149 (MQ=255)
             gtgtCAAATCCACTGTTGGATTTGCAGTTGGTGAATTCCAAAGAGAACCCTCACCACGCGTACTAAATTAAGTTCCTAGCCCAATGCAGTTGGCAACAAGAAGAAAAGAAAAAAAGGTAAAGTTAATGAGCGAAAAGCGTGCTGCTTCa                                                                                                                               >  2:2749880/1‑149 (MQ=255)
              tgtCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAGAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCAt                                                                                                                              >  1:8214383/1‑149 (MQ=255)
              tgtCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCAt                                                                                                                              <  2:141172/149‑1 (MQ=255)
              tgtCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCAt                                                                                                                              <  1:1801933/149‑1 (MQ=255)
               gtCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATg                                                                                                                             >  1:8417460/1‑149 (MQ=255)
                 cAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAg                                                                                                                           <  2:7263982/149‑1 (MQ=255)
                 cAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAg                                                                                                                           >  1:4074662/1‑149 (MQ=255)
                  aaaTCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAgg                                                                                                                          >  1:2411084/1‑149 (MQ=255)
                  aaaTCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAgg                                                                                                                          <  1:423914/149‑1 (MQ=255)
                    aTCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGc                                                                                                                        >  2:9711794/1‑149 (MQ=255)
                    aTCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGc                                                                                                                        >  1:10769457/1‑149 (MQ=255)
                         cTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACg                                                                                                                   <  2:3791057/149‑1 (MQ=255)
                         cTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACg                                                                                                                   >  1:7275062/1‑149 (MQ=255)
                         cTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACg                                                                                                                   <  1:5399259/149‑1 (MQ=255)
                          tGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGt                                                                                                                  <  1:6096303/149‑1 (MQ=255)
                           gTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGtt                                                                                                                 >  2:2173206/1‑149 (MQ=255)
                              ggATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATc                                                                                                              >  2:361555/1‑149 (MQ=255)
                              ggATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATc                                                                                                              <  1:4826756/149‑1 (MQ=255)
                                aTTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCtt                                                                                                            <  2:6997675/149‑1 (MQ=255)
                                 tttGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCttt                                                                                                           >  1:5029567/1‑149 (MQ=255)
                                  ttGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTg                                                                                                          <  2:10437749/149‑1 (MQ=255)
                                    gCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTGGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGACGACGTTATCTTTGGa                                                                                                        >  2:5848257/1‑149 (MQ=255)
                                    gCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGa                                                                                                        <  2:5051479/149‑1 (MQ=255)
                                     cAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGaa                                                                                                       <  1:2038073/149‑1 (MQ=255)
                                     cAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGaa                                                                                                       <  2:876301/149‑1 (MQ=255)
                                       gTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGACGGACGTTATCTTTGGAAAc                                                                                                     >  1:5753502/1‑149 (MQ=255)
                                        ttGGTGATTTCCACAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCACTGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACt                                                                                                    >  2:5244354/1‑149 (MQ=255)
                                          ggTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCCATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGCGGGCCGACGTTATCTTTGGAAACTcc                                                                                                  >  2:1284061/1‑149 (MQ=255)
                                           gTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCa                                                                                                 >  2:3475499/1‑149 (MQ=255)
                                           gTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCa                                                                                                 >  1:2849159/1‑149 (MQ=255)
                                           gTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCa                                                                                                 >  1:2974140/1‑149 (MQ=255)
                                            tGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCAt                                                                                                >  2:1169640/1‑149 (MQ=255)
                                            tGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCAt                                                                                                <  2:6121792/149‑1 (MQ=255)
                                             gATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCAtt                                                                                               >  1:6625425/1‑149 (MQ=255)
                                              aTTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTg                                                                                              <  1:6296383/149‑1 (MQ=255)
                                                   cAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACaaa                                                                                         <  1:7109761/149‑1 (MQ=255)
                                                      agagAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATc                                                                                      >  1:6510008/1‑149 (MQ=255)
                                                       gagaAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATca                                                                                     <  1:6855394/149‑1 (MQ=255)
                                                        agaAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAATCTCCATTGACAAAATcaa                                                                                    <  2:2994060/149‑1 (MQ=255)
                                                        agaAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATcaa                                                                                    <  1:3183915/149‑1 (MQ=255)
                                                          aaaCATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATcaaca                                                                                  >  2:6554432/1‑149 (MQ=255)
                                                               tCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCa                                                                             <  2:7587740/149‑1 (MQ=255)
                                                                   cTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCa                                                                         <  1:3877273/149‑1 (MQ=255)
                                                                    tGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCaa                                                                        <  1:9190961/149‑1 (MQ=255)
                                                                      gCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGa                                                                      >  2:1832795/1‑149 (MQ=255)
                                                                      gCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGa                                                                      <  1:9739836/149‑1 (MQ=255)
                                                                        gTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAAc                                                                    >  1:2516972/1‑149 (MQ=255)
                                                                        gTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAAc                                                                    >  1:4561461/1‑149 (MQ=255)
                                                                         tACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACCACCCACTCAAGAACa                                                                   <  2:6309561/149‑1 (MQ=255)
                                                                         tACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTCCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACa                                                                   <  1:5940133/149‑1 (MQ=255)
                                                                          aCTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACAt                                                                  <  1:1241409/149‑1 (MQ=255)
                                                                           cTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACAtt                                                                 <  1:264494/149‑1 (MQ=255)
                                                                           cTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACAtt                                                                 >  1:3600313/1‑149 (MQ=255)
                                                                           cTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACAtt                                                                 <  2:1655796/149‑1 (MQ=255)
                                                                            tAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACAttt                                                                <  1:9899313/149‑1 (MQ=255)
                                                                              aTTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTg                                                              <  2:1566444/149‑1 (MQ=255)
                                                                                  tAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTa                                                          <  2:8412579/149‑1 (MQ=255)
                                                                                     ttACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCt                                                       >  2:9534695/1‑149 (MQ=255)
                                                                                       aCTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCttt                                                     <  2:2584028/149‑1 (MQ=255)
                                                                                        cTAGCCTGACGCAGTTGACGATAAGAGGGAAAGAAAAGTGGGTAAGGTTCATGAGCGCAAAGCGTCGTGGTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAGAATCAACAACCCACTCAAGTACATTTTGTTTACCTTTg                                                    <  2:2373556/149‑1 (MQ=255)
                                                                                        cTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTg                                                    <  1:9511857/149‑1 (MQ=255)
                                                                                           gCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAATCTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGc                                                 <  1:1651297/149‑1 (MQ=255)
                                                                                               aaTGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGa                                             >  2:9036431/1‑149 (MQ=255)
                                                                                                aTGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAg                                            >  1:3469182/1‑149 (MQ=255)
                                                                                                    aGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGc                                        <  1:8078369/149‑1 (MQ=255)
                                                                                                       tGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCata                                     >  1:11306621/1‑149 (MQ=255)
                                                                                                        ggCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCatat                                    >  2:9295066/1‑149 (MQ=255)
                                                                                                        ggCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCatat                                    >  1:8625712/1‑149 (MQ=255)
                                                                                                           aaTAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGAGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTTTTTACCTTTGGGCTTGAGCGGCATATTGc                                 >  2:8219102/1‑149 (MQ=255)
                                                                                                            aTAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCg                                <  1:899690/149‑1 (MQ=255)
                                                                                                              aagaagGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGc                              <  2:7942979/149‑1 (MQ=255)
                                                                                                                gaagGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTc                            >  2:4862147/1‑149 (MQ=255)
                                                                                                                 aagGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCg                           >  2:6770150/1‑149 (MQ=255)
                                                                                                                  agGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGc                          >  2:11070186/1‑149 (MQ=255)
                                                                                                                     aaagaaaagaGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCa                       >  1:11102139/1‑149 (MQ=255)
                                                                                                                     aaagaaaagaGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCa                       >  2:10245578/1‑149 (MQ=255)
                                                                                                                      aagaaaagaGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAg                      >  2:10674229/1‑149 (MQ=255)
                                                                                                                        gaaaagaGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGaa                    >  1:10013305/1‑149 (MQ=255)
                                                                                                                        gaaaagaGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGaa                    >  2:4220440/1‑149 (MQ=255)
                                                                                                                           aagaGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATAc                 <  1:1469767/149‑1 (MQ=255)
                                                                                                                           aagaGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATAc                 >  1:3364877/1‑149 (MQ=255)
                                                                                                                            agagGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACCAAATCAACAGCCCACTCAAGAACATTTTGTTTACCTTTGGGCTTCGCCTGCATCTTGCGGCTCGCCCAGAGTAcc                >  2:7436777/1‑149 (MQ=255)
                                                                                                                             gagGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCa               >  1:7795668/1‑149 (MQ=255)
                                                                                                                               gggTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGa             >  1:10446637/1‑149 (MQ=255)
                                                                                                                               gggTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGa             <  1:2183101/149‑1 (MQ=255)
                                                                                                                                ggTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGAc            <  2:5610500/149‑1 (MQ=255)
                                                                                                                                ggTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGAc            <  2:4538519/149‑1 (MQ=255)
                                                                                                                                ggTAAAGTTCATGAGCGCAAAGCGTGGGGCTTCATGAGGGCCGACGATAACTTTGAAAACACCATTGACCAAATCAAGAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAACTGCATAATGCGGCTCGCCCAGAATACCAGAc            >  2:328810/1‑149 (MQ=255)
                                                                                                                                 gTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGAcc           <  1:5260317/149‑1 (MQ=255)
                                                                                                                                    aaGTTCATGAGCGCAAAGCGTTGTGCTCCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGaa        >  2:2041641/1‑149 (MQ=255)
                                                                                                                                    aaGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGaa        >  2:8850946/1‑149 (MQ=255)
                                                                                                                                      gTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGaaaa      >  2:7813545/1‑149 (MQ=255)
                                                                                                                                               ||                                                                                                                                              
ACAAGAAGTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGGCCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGAAAACAAT  >  AM406671/1933071‑1933357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑