Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 1,223,555 Δ1 bp intergenic (+243/+302) llmg_1251 → / ← llmg_1252 hypothetical protein predicted by Glimmer/Critica/conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM4066711,223,5550G.92.8% 2544.8 / 215.6 668intergenic (+243/+302)llmg_1251/llmg_1252hypothetical protein predicted by Glimmer/Critica/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  major base . (262/354);  minor base T (21/27);  total (285/383)
Fisher's exact test for biased strand distribution p-value = 8.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 669 total aligned reads displayed.

AGGGTCAGTATTAGGGTCGGTATTAGGGTCAGTATTAGGGTCGGTATTGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAAGTGATATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCGTTATTATTTTAATCGTTAAAATTAAGATAAGAATCTTT  >  AM406671/1223408‑1223694
                                                                                                                                                   |                                                                                                                                           
           tagggtcGGTATTAGGGTCAGTATTAGGGTCGGTATTGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAAt                                                                                                                                >  2:1880294/1‑149 (MQ=255)
              ggtcGGTATTAGGGTCAGTATTAGGGTCGGTATTGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAAtttt                                                                                                                             <  1:8294180/149‑1 (MQ=255)
               gtcGGTATTAGGGTCAGTATTAGGGTCGGTATTGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTa                                                                                                                            <  1:1266128/149‑1 (MQ=255)
                   gtattagggtcggtattagggtcgGTATTGAGGTCAGTATTGAGGTTAGTATCGCGGTCAGTATTCAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCACTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATAc                                                                                                                        <  2:8892840/149‑1 (MQ=21)
                      ttAGGGTCAGTATTAGGGTCGGTATTGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAg                                                                                                                     <  1:4727617/149‑1 (MQ=255)
                       tAGGGTCAGTATTAGGGTCGGTATTGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGa                                                                                                                    >  2:6502888/1‑149 (MQ=255)
                       tAGGGTCAGTATTAGGGTCGGTATTGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGa                                                                                                                    >  2:8276946/1‑149 (MQ=255)
                       tAGGGTCAGTATTAGGGTCGGTATTGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGa                                                                                                                    >  1:5667991/1‑149 (MQ=255)
                        aGGTTCAGTATTAGGGTCGGTATTGAGGTCAGTATTGAGGTTAGTATTGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGAc                                                                                                                   <  2:7385234/149‑1 (MQ=21)
                         gggTCAGTATTAGGGTCGGTATTGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGAcc                                                                                                                  >  1:4321872/1‑149 (MQ=255)
                                tATTGGGGTCGGTATTGGGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACaaaa                                                                                                           <  2:7249370/149‑1 (MQ=25)
                                tATTAGGGTCGGTATTGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACaaaa                                                                                                           >  2:2711372/1‑149 (MQ=255)
                                tATTAGGGTCGGTATTGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACaaaa                                                                                                           <  2:6985532/149‑1 (MQ=255)
                                  ttAGGGTCGGTATTGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAAt                                                                                                         >  1:1010116/1‑149 (MQ=255)
                                  ttAGGGTCGGTATTGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAAt                                                                                                         >  1:5449989/1‑149 (MQ=255)
                                   tAGGGTCGGTATTGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAAtt                                                                                                        <  1:3968629/149‑1 (MQ=255)
                                   tAGGGTCGGTATTGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAAtt                                                                                                        >  1:721293/1‑149 (MQ=255)
                                    aGGGTCGGTATTGAGGTCAGTATTTAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCAGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAAttt                                                                                                       <  2:4096774/149‑1 (MQ=25)
                                    aGGGTCGGTATTGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAAttt                                                                                                       >  1:7734066/1‑149 (MQ=255)
                                      ggTCGTTATTGAGGTTAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTtat                                                                                                     <  1:9101982/149‑1 (MQ=37)
                                       gTCGTTATTGGGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTtata                                                                                                    <  1:4877005/149‑1 (MQ=37)
                                        tCGGTTTTGAGGTGAGTATTGGGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAg                                                                                                   <  1:8838852/149‑1 (MQ=25)
                                          ggTATTGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCt                                                                                                 <  1:1455409/149‑1 (MQ=255)
                                          ggTATTGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCt                                                                                                 <  1:3785611/149‑1 (MQ=255)
                                           gTATTGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTa                                                                                                <  2:3627184/149‑1 (MQ=255)
                                           gTATTGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTa                                                                                                >  2:8531644/1‑149 (MQ=255)
                                           gTATTGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTa                                                                                                >  2:7701702/1‑149 (MQ=255)
                                           gTATTGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTACTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTa                                                                                                <  1:6679829/149‑1 (MQ=38)
                                            tATTGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTAt                                                                                               <  1:4077918/149‑1 (MQ=255)
                                             aTTGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATg                                                                                              <  1:2343760/149‑1 (MQ=255)
                                              ttGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGc                                                                                             <  2:5353404/149‑1 (MQ=255)
                                               tGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCt                                                                                            >  2:4784391/1‑149 (MQ=255)
                                                  ggTCAGTTTTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTg                                                                                         <  2:4970660/149‑1 (MQ=37)
                                                   gTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTga                                                                                        <  1:5410310/149‑1 (MQ=255)
                                                   gTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTga                                                                                        <  2:5647064/149‑1 (MQ=255)
                                                      aGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAg                                                                                                   <  2:1671549/135‑1 (MQ=255)
                                                      aGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAg                                                                                                   >  1:1671549/1‑135 (MQ=255)
                                                         aTTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGaaaaa                                                                                  >  1:2503083/1‑149 (MQ=255)
                                                          ttGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAg                                                                                 <  2:7479973/149‑1 (MQ=255)
                                                          ttGAGGGTAGGAACAGGGGCCGTATTGAGGTCTGTATTAGGGTTAGTATTAGGGTCGATATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATTCTTTGAGaaaaat                                                                                 >  2:3819102/1‑148 (MQ=25)
                                                           tGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGc                                                                                <  1:5246139/149‑1 (MQ=255)
                                                            gAGGTTAGTATCGGGGTCAGTATTGACGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCa                                                                               <  1:5880116/149‑1 (MQ=38)
                                                              ggTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCaaa                                                                             <  2:5470656/149‑1 (MQ=255)
                                                                 tAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACtt                                                                          <  1:1824046/149‑1 (MQ=255)
                                                                 tAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACtt                                                                          >  2:617240/1‑149 (MQ=255)
                                                                 tAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTAAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACtt                                                                          <  2:6055271/149‑1 (MQ=38)
                                                                  aGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACttt                                                                         <  1:4994036/149‑1 (MQ=255)
                                                                     aTCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTc                                                                      >  2:3098170/1‑149 (MQ=255)
                                                                       cGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGt                                                                    >  2:4293973/1‑149 (MQ=255)
                                                                       cGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGt                                                                    >  1:5546942/1‑149 (MQ=255)
                                                                         gggTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTaa                                                                  <  1:622843/149‑1 (MQ=255)
                                                                         gggTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTaa                                                                  <  2:2294528/149‑1 (MQ=255)
                                                                           gTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAg                                                                <  1:7111648/149‑1 (MQ=255)
                                                                           gTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAg                                                                <  1:7522598/149‑1 (MQ=255)
                                                                              aGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGata                                                             >  2:9221944/1‑149 (MQ=255)
                                                                               gTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGatat                                                            <  2:6320543/149‑1 (MQ=255)
                                                                               gTATTGAGGTCAGTATTAAGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGatat                                                            <  1:5806493/149‑1 (MQ=255)
                                                                                 aTTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATAc                                                          >  1:3118165/1‑149 (MQ=255)
                                                                                  ttGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATAcc                                                         <  2:5565171/149‑1 (MQ=255)
                                                                                  ttGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATAcc                                                         <  1:8593979/149‑1 (MQ=255)
                                                                                   tGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCt                                                        >  1:4564392/1‑149 (MQ=255)
                                                                                          aGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAAt                                                 <  2:2851683/149‑1 (MQ=255)
                                                                                            tATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATga                                               >  2:1186831/1‑149 (MQ=255)
                                                                                            tATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATga                                               <  2:7636500/149‑1 (MQ=255)
                                                                                             aTTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATgag                                              <  1:264944/149‑1 (MQ=255)
                                                                                              ttAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATgaga                                             >  1:7862290/1‑149 (MQ=255)
                                                                                              ttAGGGTTAGTATTAGGATCGTTATTAGGGTCAATATTAAGATCAGTATTAAATAGTATTATAAATTTTAATACAAGACCTACAAAAATTTATAGATAAGCTTTAAGAAAAAGCAAACTTTTTCGTAAAAAAATAAAAAAAAAATgaga                                             <  2:1427700/149‑1 (MQ=21)
                                                                                              ataagtttAGTATTAGGGACAGTATTAGGATCAGTATTAAGGTAAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATgaga                                             <  2:4488656/143‑1 (MQ=21)
                                                                                               tAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATgagag                                            >  1:7998208/1‑149 (MQ=255)
                                                                                               tAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATgagag                                            <  1:2858058/149‑1 (MQ=255)
                                                                                               tAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATgagag                                            >  2:8622451/1‑149 (MQ=255)
                                                                                                 gggTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGagagag                                          >  2:3005271/1‑149 (MQ=255)
                                                                                                   gTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCg                                        >  1:1547015/1‑149 (MQ=255)
                                                                                                   gTTAGTATTAGGGTCGGTATTAGGGTCACTATTAAGGTCCGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCg                                        <  1:6619147/149‑1 (MQ=37)
                                                                                                   gTTAGTATTAGGGTCAGTATTAGGGTCAGTATTAAGGTCAAAAATAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTAAGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATAAAAAAAAAAAATGAGAGAGCg                                        <  2:5183187/149‑1 (MQ=21)
                                                                                                     tAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCGtt                                      >  2:7121126/1‑149 (MQ=255)
                                                                                                         aTTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCGttatta                                  >  1:6860901/1‑149 (MQ=255)
                                                                                                         aTTAGGGTCGGTATTAGGGTCAGTACTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCGttatta                                  >  2:5043780/1‑149 (MQ=38)
                                                                                                            aGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCGTTATTAttt                               <  1:933578/149‑1 (MQ=255)
                                                                                                              ggTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCGTTATTATTTTa                             >  1:8961173/1‑149 (MQ=255)
                                                                                                              ggTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCGTTATTATTTTa                             >  1:3285784/1‑149 (MQ=255)
                                                                                                              ggTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCGTTATTATTTTa                             <  1:7599787/149‑1 (MQ=255)
                                                                                                              ggTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCGTTATTATTTTa                             <  1:7358221/149‑1 (MQ=255)
                                                                                                              ggTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCGTTATTATTTTa                             >  1:7016757/1‑149 (MQ=255)
                                                                                                                  ggTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCGTTATTATTTTAATCg                         <  2:5989310/149‑1 (MQ=255)
                                                                                                                  ggTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCAAAACTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCGTTATTATTTTAATCg                         <  2:9096358/149‑1 (MQ=25)
                                                                                                                   gTATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTTTAATACTAGACCTACAAACATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACATAAAAAATGAGAGAGCGTTATTATTTTAATAGt                        >  2:3949354/1‑149 (MQ=37)
                                                                                                                    tATTAGGGTCAGTATTAAGGTCAGTATTAAATAGTATTATTAATTATAATACTAGAGCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACATTTTCGTAAAGATATACGTAAAAAATGAGAGAGCGTTATTATTTTACTCGtt                       >  2:6707727/1‑149 (MQ=25)
                                                                                                                            tcagtattaaggtcagtattaaATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCGTTATTATTTTAATCGTTAAAATtaa               >  2:687521/1‑149 (MQ=255)
                                                                                                                             cagtattaaggtcagtattaaATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCGTTATTATTTTAATCGTTAAAATtaag              <  2:2175157/149‑1 (MQ=255)
                                                                                                                               gtattaaggtcagtattaaATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCGTTATTATTTTAATCGTTAAAATtaagat            >  1:2191291/1‑149 (MQ=255)
                                                                                                                               gtattaaggtcagtattaaATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCGTTATTATTTTAATCGTTAAAATtaagat            <  2:7989399/149‑1 (MQ=255)
                                                                                                                                  ttaaggtcagtattaaATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCGTTATTATTTTAATCGTTAAAATtaagataag         <  2:778300/149‑1 (MQ=255)
                                                                                                                                   taaggtcagtattaaATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCGTTATTATTTTAATCGTTAAAATtaagataaga        <  1:3603715/149‑1 (MQ=255)
                                                                                                                                   taaggtcagtattaaATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCGTTATTATTTTAATCGTTAAAATtaagataaga        >  1:8137683/1‑149 (MQ=255)
                                                                                                                                   taaggtcagtattaaATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCGTTATTATTTTAATCGTTAAAATtaagataaga        <  2:2529374/149‑1 (MQ=255)
                                                                                                                                      ggtcagtattaaATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCGTTATTATTTTAATCGTTAAAATTAAGATAAGAATc     <  1:2027128/149‑1 (MQ=255)
                                                                                                                                        tcagtattaaATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCGTTATTATTTTAATCGTTAAAATTAAGATAAGAATCtt   <  1:636621/149‑1 (MQ=255)
                                                                                                                                        tcagtattaaATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCGTTATTATTNTAATCGTTAAAATTAAGATAAGAATCtt   <  1:5101895/149‑1 (MQ=255)
                                                                                                                                        tcagtattaaATAGTATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTAGGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCGTTATTATTTTAATCGTTAAAATTAAGATAAGAATCtt   <  2:8387306/149‑1 (MQ=39)
                                                                                                                                                   |                                                                                                                                           
AGGGTCAGTATTAGGGTCGGTATTAGGGTCAGTATTAGGGTCGGTATTGAGGTCAGTATTGAGGTTAGTATCGGGGTCAGTATTGAGGTCAGTATTAGGGTTAGTATTAGGGTCGGTATTAGGGTCAGTATTAAGGTCAGTATTAAAGTGATATTATTAATTTTAATACTAGACCTACAAAAATTTATAGCTATGCTTTGAGAAAAAGCAAACTTTTTCGTAAAGATATACCTAAAAAATGAGAGAGCGTTATTATTTTAATCGTTAAAATTAAGATAAGAATCTTT  >  AM406671/1223408‑1223694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: