BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,916,379	Δ1 bp	pseudogene (355/598 nt)	llmg_pseudo_59 ←	pseudogene

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,916,379	0	T	.	99.7%	4639.5 / 1.9	977	pseudogene (355/598 nt)	llmg_pseudo_59	pseudogene
Reads supporting (aligned to +/- strand): ref base T (3/0); new base . (514/460); total (517/460)
Fisher's exact test for biased strand distribution p-value = 2.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 989 total aligned reads displayed.

CGCGGCTTCAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATATGGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCATTTTTAGATG  >  AM406671/1916235‑1916522

  cgGCTTCAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑Ggaagaa                                                                                                                                          <  1:1484353/149‑1 (MQ=255)

     cTTCAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAAtt                                                                                                                                       <  1:3150651/149‑1 (MQ=255)

        cAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCa                                                                                                                                    >  1:811218/1‑149 (MQ=255)

          atatCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAg                                                                                                                                  <  1:6040839/149‑1 (MQ=255)

                aaTTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGttt                                                                                                                            >  1:7786661/1‑149 (MQ=255)

                aaTTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGttt                                                                                                                            >  2:6053008/1‑149 (MQ=255)

                    ttCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAAGAACTTCTAAAAAAGA‑GGAAGAAATTCCAAGTTGTTTgtgg                                                                                                                        >  2:8787849/1‑146 (MQ=255)

                    ttCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGt                                                                                                                        >  1:6159796/1‑149 (MQ=255)

                        gTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAAt                                                                                                                    <  2:8616693/149‑1 (MQ=255)

                           aCCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCaa                                                                                                                 <  2:2904188/149‑1 (MQ=255)

                              gATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAgg                                                                                                              >  2:5254254/1‑149 (MQ=255)

                                tataGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTATGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCa                                                                                                            >  2:4085880/1‑149 (MQ=255)

                                 ataGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCAt                                                                                                           <  2:7553231/149‑1 (MQ=255)

                                 ataGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCAt                                                                                                           >  1:1718639/1‑149 (MQ=255)

                                   agtagGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCAttt                                                                                                         <  1:705255/149‑1 (MQ=255)

                                    gtagGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCAtttt                                                                                                        <  1:8904432/149‑1 (MQ=255)

                                    gtagGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCAtttt                                                                                                        >  1:3548291/1‑149 (MQ=255)

                                          ttCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCtt                                                                                                  <  2:8318001/149‑1 (MQ=255)

                                            catcaGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTtg                                                                                                <  2:2735262/149‑1 (MQ=255)

                                                aGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAg                                                                                            >  1:5357388/1‑149 (MQ=255)

                                                  tCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGta                                                                                          >  1:7304379/1‑149 (MQ=255)

                                                  tCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGta                                                                                          >  1:515768/1‑149 (MQ=255)

                                                  tCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATAAAAGGCATTTTTGCCTTTGTGAGta                                                                                          <  1:240567/149‑1 (MQ=255)

                                                   cTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGtat                                                                                         >  2:1845767/1‑149 (MQ=255)

                                                   cTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGtat                                                                                         >  2:6874716/1‑149 (MQ=255)

                                                   cTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGtat                                                                                         <  1:6294938/149‑1 (MQ=255)

                                                    tttAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGtata                                                                                        >  2:2376774/1‑149 (MQ=255)

                                                     ttAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATaa                                                                                       >  1:1364810/1‑149 (MQ=255)

                                                      tAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATaaa                                                                                      <  1:1000237/149‑1 (MQ=255)

                                                      gaaaTGATATTTTTGAACTAAGTAAGTGAATGCCTCAGGAGTTGGTTTTCTGTTAAAATTATTTTCAGAGGAAAAAACAACAAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATaaa                                                                                      <  2:5993971/148‑1 (MQ=255)

                                                       aaaTTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAAt                                                                                     >  2:1465693/1‑149 (MQ=255)

                                                       aaaTTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAAt                                                                                     <  2:6413136/149‑1 (MQ=255)

                                                       aaaTTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAAt                                                                                     >  1:6752137/1‑149 (MQ=255)

                                                        aaTTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATg                                                                                    <  1:3961733/149‑1 (MQ=255)

                                                         aTTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCTTTTGTGTTTATAAATGa                                                                                   >  2:3136078/1‑149 (MQ=255)

                                                         aTTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCGTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGa                                                                                   >  2:4688036/1‑149 (MQ=255)

                                                          ttATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGaa                                                                                  >  1:6903336/1‑149 (MQ=255)

                                                          ttATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGaa                                                                                  >  1:4562351/1‑149 (MQ=255)

                                                          ttATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGaa                                                                                  <  2:456761/149‑1 (MQ=255)

                                                            atatTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGt                                                                                >  1:8677036/1‑149 (MQ=255)

                                                            atatTTTTTAACTAAGTAACTGATTTCCTCAGGATTTGGTTTTCTTTTAAAATTATTATCAGACGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGt                                                                                <  2:5447112/149‑1 (MQ=255)

                                                            agatTTTTTAACTAAGTTATGAATTGCCGCAGGTATTGGGTTTCTTTGAATATTATGTAAAGAGGTAACTACTACGAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCGAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGt                                                                                <  2:7055571/147‑1 (MQ=255)

                                                                tttttAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTg                                                                            >  2:3378472/1‑149 (MQ=255)

                                                                  tttAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGaa                                                                          <  1:4053366/149‑1 (MQ=255)

                                                                  tttAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGaa                                                                          >  2:1720312/1‑149 (MQ=255)

                                                                    tAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAGCTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATg                                                                        >  1:4785876/1‑149 (MQ=255)

                                                                    tAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATg                                                                        >  2:3689625/1‑149 (MQ=255)

                                                                    tAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATg                                                                        <  1:2292155/149‑1 (MQ=255)

                                                                     aaCTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGt                                                                       <  1:5614289/149‑1 (MQ=255)

                                                                       cTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGttt                                                                     >  1:1299575/1‑149 (MQ=255)

                                                                        tAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGtttt                                                                    >  2:8006654/1‑149 (MQ=255)

                                                                         aaGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTc                                                                   <  1:4908845/149‑1 (MQ=255)

                                                                             aaTTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCtttt                                                               >  2:1959141/1‑149 (MQ=255)

                                                                             aaTTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCtttt                                                               <  1:7181217/149‑1 (MQ=255)

                                                                             aaTTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCtttt                                                               <  1:97825/149‑1 (MQ=255)

                                                                              attgattgCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCttttt                                                              >  1:9201063/1‑149 (MQ=255)

                                                                               ttgattaCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTGACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGCTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGCTTTGAATGTTTTCTTTTTg                                                             >  2:4460014/1‑149 (MQ=255)

                                                                                tgattgCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGa                                                            >  1:296876/1‑149 (MQ=255)

                                                                                tgattgCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGa                                                            >  1:4221443/1‑149 (MQ=255)

                                                                                 gattgCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAg                                                           >  2:2186803/1‑149 (MQ=255)

                                                                                   ttgCCTCAGGATTTGGTTTTCTTTTACAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCGTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGtc                                                         <  1:5879303/149‑1 (MQ=255)

                                                                                     gCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGtcca                                                       <  1:3760853/149‑1 (MQ=255)

                                                                                      ccTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGtctaa                                                      >  2:721461/1‑149 (MQ=255)

                                                                                      ccTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGtccaa                                                      <  1:5476107/149‑1 (MQ=255)

                                                                                       cTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGtccaat                                                     <  2:2092556/149‑1 (MQ=255)

                                                                                        tCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGtccaatc                                                    >  2:2477323/1‑149 (MQ=255)

                                                                                          aGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGtccaatcca                                                  >  2:3532835/1‑149 (MQ=255)

                                                                                          aGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGtccaatcca                                                  >  2:5667489/1‑149 (MQ=255)

                                                                                          aGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGtccaatcca                                                  <  2:479874/149‑1 (MQ=255)

                                                                                          aGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGtccaatcca                                                  >  1:1155214/1‑149 (MQ=255)

                                                                                            gATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCaaa                                                <  1:8792850/149‑1 (MQ=255)

                                                                                             aTTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCaaaa                                               <  1:3235392/149‑1 (MQ=255)

                                                                                                  gTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAActtct                                          <  1:7530511/149‑1 (MQ=255)

                                                                                                   ttttCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAActtctt                                         <  1:8420018/149‑1 (MQ=255)

                                                                                                   ttttCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAActtctt                                         <  2:6238332/149‑1 (MQ=255)

                                                                                                    tttCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCttt                                        <  1:4380307/149‑1 (MQ=255)

                                                                                                      tCTTTTAAAATTATTTTCAGAGGTAACAAATTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAg                                      <  2:7766626/149‑1 (MQ=255)

                                                                                                       cTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTTCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGc                                     >  2:1266197/1‑149 (MQ=255)

                                                                                                        ttttAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCa                                    <  2:6712045/149‑1 (MQ=255)

                                                                                                        ttttAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCa                                    <  2:3885348/149‑1 (MQ=255)

                                                                                                         tttAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCAc                                   >  2:842309/1‑149 (MQ=255)

                                                                                                         tttAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCAc                                   >  1:1871334/1‑149 (MQ=255)

                                                                                                           tAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAATTTCTTTAGCACCt                                 >  2:1133465/1‑149 (MQ=255)

                                                                                                           tAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCt                                 <  1:8235571/149‑1 (MQ=255)

                                                                                                           tAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑AAAAAAAATTCCAAGTAGTTTGAGTAAATCAAAGGCATAATTACCTTTGTAAGTATAAATGAACTCTAAAATTAGATCTTTCTGAGTCCAAACCAAAATTCCTCTATCACCt                                 >  2:4288073/1‑149 (MQ=255)

                                                                                                            aaaaTTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTTTTTTTTTTTTTATAAATGAAGTTTTGAATGTTTTCTTTTTGTTTCCAATCCAAAACTTCTTTAGctcttt                                >  2:7322943/1‑144 (MQ=255)

                                                                                                             aaaTTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTAc                               >  1:9032533/1‑149 (MQ=255)

                                                                                                               attattTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCa                             <  1:7991888/149‑1 (MQ=255)

                                                                                                                ttattTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCAt                            >  2:7478336/1‑149 (MQ=255)

                                                                                                                   ttttCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTaa                         <  1:3349771/149‑1 (MQ=255)

                                                                                                                      tCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAattt                      <  2:9064604/149‑1 (MQ=255)

                                                                                                                       cAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAatttt                     <  1:6611091/149‑1 (MQ=255)

                                                                                                                           ggTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAattttattt                 >  2:6551939/1‑149 (MQ=255)

                                                                                                                            gTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAattttatttt                <  1:5017236/149‑1 (MQ=255)

                                                                                                                                   cTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCAttt         >  1:6414475/1‑149 (MQ=255)

                                                                                                                                   cTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCAttt         <  1:2738438/149‑1 (MQ=255)

                                                                                                                                    ttCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTa                          <  1:2592065/131‑1 (MQ=255)

                                                                                                                                      ccAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCATTTTTa      >  2:5793599/1‑149 (MQ=255)

                                                                                                                                          aaaaTA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCATTTTTAGATg  <  1:2086768/149‑1 (MQ=255)

                                                                                                                                          aaaaTA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTGTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCTAAAACTTCTTTAGCTCCTACCATTATTTTTATTTTGTCATTTTTAGATg  <  2:5024336/149‑1 (MQ=255)

CGCGGCTTCAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATATGGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCATTTTTAGATG  >  AM406671/1916235‑1916522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`