BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,921,652	Δ1 bp	intergenic (‑142/‑243)	llmg_1941 ← / → glnP	conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,921,651	0	T	.	99.9%	4465.4 / ‑2.1	938	intergenic (‑141/‑244)	llmg_1941/glnP	conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base . (517/419); total (518/420)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 1007 total aligned reads displayed.

GTACAAACATAATATCCCTTTTCAAAAGTTTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAACTTTTCTTATATTTTATTCAGGAGAACTCTTCAAATTTCAACAAATTTCAAAATGTCAGGAAAACCTAAACATTCAACTTGATTTCTAGAACAATTTTTGTGATATAATTTTTCCCAGAAATAAAATAAACTGAGCTTGTCTTCCTAAATATAGGGAAACTAAAAGAATGTT  >  AM406671/1921501‑1921806

gTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTAt                                                                                                                                                             <  2:4036284/149‑1 (MQ=255)

   cAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTCTATTTTAttag                                                                                                                                                          >  1:5367696/1‑147 (MQ=255)

   cAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTAtcag                                                                                                                                                          >  1:6610553/1‑146 (MQ=255)

   cAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTAtcag                                                                                                                                                          <  2:837775/149‑4 (MQ=255)

    aaaCATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTAtcaga                                                                                                                                                         >  1:4889636/1‑145 (MQ=255)

           atatTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAActct                                                                                                                                                <  2:2931606/149‑1 (MQ=255)

           atatTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAActct                                                                                                                                                <  1:4086800/149‑1 (MQ=255)

            tattccTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAACTATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAActctc                                                                                                                                               >  1:4788032/5‑148 (MQ=255)

            tattccTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAActctc                                                                                                                                               >  2:6499561/5‑148 (MQ=255)

             attccTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCTCTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAActctca                                                                                                                                              <  2:1871113/146‑3 (MQ=255)

             attccTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAActctca                                                                                                                                              >  2:8557687/4‑147 (MQ=255)

              ttccTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAActctcaa                                                                                                                                             <  2:3639779/147‑4 (MQ=255)

                 cTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACCTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑Tcaaatt                                                                                                                                         >  2:4241848/1‑149 (MQ=255)

                    ttCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑Tcaaatttca                                                                                                                                      <  2:5964014/149‑1 (MQ=255)

                     tCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑Tcaaatttcac                                                                                                                                     >  2:8787317/1‑148 (MQ=255)

                       aaaaG‑TTAAAAATTCTTACAGTAGTTCTGTTTAGTTAAATAGAATACATAAATATCTATATCAAGCACTCGTTCAATTTTACAAGAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACAC‑Tcaaatttcacaa                                                                                                                                   >  2:755620/1‑146 (MQ=255)

                       aaaaG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑Tcaaatttcacaa                                                                                                                                   <  1:6399688/149‑4 (MQ=255)

                            gttAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑tcaaatttc‑acaaattc                                                                                                                              >  2:6271122/2‑148 (MQ=255)

                             ttAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑tcaaatttc‑acaaattca                                                                                                                             <  1:3232060/149‑3 (MQ=255)

                             ttAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTAAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑tcaaatttc‑acaaattca                                                                                                                             >  2:1548475/1‑147 (MQ=255)

                                aaaaTTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCaaaa                                                                                                                         >  1:1957971/1‑149 (MQ=255)

                                 aaaTTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAAt                                                                                                                        >  1:5246198/1‑149 (MQ=255)

                                    ttCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTc                                                                                                                     >  2:8248749/1‑149 (MQ=255)

                                    ttCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTc                                                                                                                     <  1:2534168/149‑1 (MQ=255)

                                     tCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCa                                                                                                                    >  2:6063530/1‑149 (MQ=255)

                                      cTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCAg                                                                                                                   <  1:3744899/149‑1 (MQ=255)

                                      cTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCAg                                                                                                                   <  2:4142665/149‑1 (MQ=255)

                                       ttACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAActctc                                                                                                                                               <  2:7939132/123‑2 (MQ=255)

                                       ttACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCAga                                                                                                                  >  1:61167/1‑148 (MQ=255)

                                       ttACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCAga                                                                                                                  <  1:1287561/149‑2 (MQ=255)

                                        tACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCAgaa                                                                                                                 >  2:3300162/1‑147 (MQ=255)

                                        tACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCAgaa                                                                                                                 >  1:7104831/1‑147 (MQ=255)

                                        tACAGTAGTGCTGTTTACTTAAATAGAATACATACATATCTCTATCCAGCACTCGTTCAATTTTACCACAAAAAGGACTTTTTGACAATATAAAC‑CTTCTTATATTTTA‑TCA‑GAGAACTC‑TCACATTTC‑ACAAA‑TTCATAACGTCAgaa                                                                                                                 >  2:8911158/1‑147 (MQ=255)

                                           agtagtTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAAc                                                                                                             >  2:2532590/1‑149 (MQ=255)

                                           agtagtTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAAc                                                                                                             <  2:2196191/149‑1 (MQ=255)

                                            gtagtTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAAcc                                                                                                            >  1:1813658/1‑149 (MQ=255)

                                             tagtTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCt                                                                                                           >  2:2004445/1‑149 (MQ=255)

                                              agtTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCTa                                                                                                          <  2:5223251/149‑1 (MQ=255)

                                               gtTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCTaa                                                                                                         >  2:387019/1‑149 (MQ=255)

                                               gtTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCTaa                                                                                                         <  1:3962940/149‑1 (MQ=255)

                                                     tttACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCTaacatcaa                                                                                                   <  2:1407929/149‑7 (MQ=255)

                                                        aCTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑Tcaaatttcacaa                                                                                                                                   <  2:9007324/117‑4 (MQ=255)

                                                         cTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTg                                                                                             <  1:4223037/149‑1 (MQ=255)

                                                          ttAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGa                                                                                            >  2:9164895/1‑149 (MQ=255)

                                                             aaTAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGAttc                                                                                         <  2:312005/149‑2 (MQ=255)

                                                             aaTAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGAttc                                                                                         <  2:1136038/149‑2 (MQ=255)

                                                              aTAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTTACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGAttct                                                                                        >  2:6628320/1‑147 (MQ=255)

                                                              aTAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTG‑TTTCt                                                                                       >  1:2192346/1‑149 (MQ=255)

                                                                 gAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAGa                                                                                    >  1:33453/1‑149 (MQ=255)

                                                                   aTACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAGaca                                                                                  <  1:1436534/149‑3 (MQ=255)

                                                                   aTACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAGaca                                                                                  >  1:4390005/1‑147 (MQ=255)

                                                                     aCATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAGacaat                                                                                <  2:4533298/149‑5 (MQ=255)

                                                                     aCATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAGacaat                                                                                >  1:7257836/1‑145 (MQ=255)

                                                                      cATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAAtt                                                                              <  2:4911227/149‑1 (MQ=255)

                                                                       aTAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAAttt                                                                             <  1:8024488/149‑1 (MQ=255)

                                                                         aaaTATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAAttttt                                                                           >  1:8135071/1‑149 (MQ=255)

                                                                           atatCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTtgt                                                                         <  1:4500174/149‑1 (MQ=255)

                                                                            tatCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GCAAACCT‑AACA‑TCAACTTGA‑TTCTCG‑ACAATTTTtgtg                                                                        >  2:5132754/1‑149 (MQ=255)

                                                                              tctcTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGat                                                                      <  1:3500337/149‑1 (MQ=255)

                                                                               ctctATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGata                                                                     >  2:4349285/1‑149 (MQ=255)

                                                                                  tATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATaa                                                                  <  2:2364654/149‑1 (MQ=255)

                                                                                    tCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAtt                                                                >  2:6942341/1‑149 (MQ=255)

                                                                                       aGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AAGA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAttttc                                                             <  2:5547977/149‑2 (MQ=255)

                                                                                        gCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAttttcc                                                            >  2:471147/1‑147 (MQ=255)

                                                                                        gCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAttttcc                                                            <  1:7754485/149‑3 (MQ=255)

                                                                                        gCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATCTTTGTGATATAAttttcc                                                            >  2:2677413/1‑147 (MQ=255)

                                                                                         cACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAATTTTccc                                                           <  1:6994086/149‑1 (MQ=255)

                                                                                           cTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAg                                                        >  1:503345/1‑149 (MQ=255)

                                                                                             cGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAGaa                                                      <  1:4624889/149‑1 (MQ=255)

                                                                                                tCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAGaataa                                                   >  1:6809920/1‑146 (MQ=255)

                                                                                                tCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAGaataa                                                   <  1:2881184/149‑4 (MQ=255)

                                                                                                     tttACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑aataaaataa                                             <  1:7884868/149‑1 (MQ=255)

                                                                                                       tACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCTAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAAc                                           <  1:5588108/149‑1 (MQ=255)

                                                                                                          aacaaAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGa                                        >  2:3456582/1‑149 (MQ=255)

                                                                                                          aacaaAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGa                                        >  1:5469626/1‑149 (MQ=255)

                                                                                                            caaAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGc                                      >  2:1683410/1‑149 (MQ=255)

                                                                                                               aaaGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTg                                   >  2:5866599/1‑149 (MQ=255)

                                                                                                               aaaGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACGC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTg                                   >  1:767121/1‑149 (MQ=255)

                                                                                                                aaGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGAttcta                                                                                       <  2:773217/100‑4 (MQ=255)

                                                                                                                aaGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGt                                  >  2:2844943/1‑149 (MQ=255)

                                                                                                                      tttttGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTCtccta                            >  1:2389531/1‑145 (MQ=255)

                                                                                                                      tttttGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTCtccta                            >  1:8888010/1‑145 (MQ=255)

                                                                                                                       ttttGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTaa                          <  1:8683319/149‑1 (MQ=255)

                                                                                                                         ttGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAat                        >  2:5694608/1‑149 (MQ=255)

                                                                                                                         ttGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑GCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAat                        <  1:9126158/149‑1 (MQ=255)

                                                                                                                          tGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAata                       <  1:7391067/149‑1 (MQ=255)

                                                                                                                           gACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAatat                      <  1:1627867/149‑1 (MQ=255)

                                                                                                                           gACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAatat                      >  2:7114691/1‑149 (MQ=255)

                                                                                                                                  taAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAggaaac               <  2:5431512/149‑5 (MQ=255)

                                                                                                                                  taAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAggaaac               >  2:7200319/1‑145 (MQ=255)

                                                                                                                                     aactttCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTa            >  1:6210514/4‑149 (MQ=255)

                                                                                                                                      actttCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTaa           <  1:1005215/147‑1 (MQ=255)

                                                                                                                                       ctttCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTaaa          >  1:8448074/2‑149 (MQ=255)

                                                                                                                                         ttCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAg        >  1:8974039/1‑149 (MQ=255)

                                                                                                                                         ttCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAg        >  1:8306959/1‑149 (MQ=255)

                                                                                                                                             tataTTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATg    >  1:2985498/1‑149 (MQ=255)

                                                                                                                                             tataTTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATg    >  2:7375423/1‑149 (MQ=255)

                                                                                                                                               tatTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGtt  >  1:2698206/1‑149 (MQ=255)

                                                                                                                                               tatTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGtt  <  1:5939304/149‑1 (MQ=255)

                                                                                                                                               tatTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGtt  >  1:962609/1‑149 (MQ=255)

GTACAAACATAATATCCCTTTTCAAAAGTTTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAACTTTTCTTATATTTTATTCAGGAGAACTCTTCAAATTTCAACAAATTTCAAAATGTCAGGAAAACCTAAACATTCAACTTGATTTCTAGAACAATTTTTGTGATATAATTTTTCCCAGAAATAAAATAAACTGAGCTTGTCTTCCTAAATATAGGGAAACTAAAAGAATGTT  >  AM406671/1921501‑1921806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`