BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,921,747	(T)_5→4	intergenic (‑237/‑148)	llmg_1941 ← / → glnP	conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,921,743	0	T	.	98.0%	4283.4 / 66.7	940	intergenic (‑233/‑152)	llmg_1941/glnP	conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein
Reads supporting (aligned to +/- strand): ref base T (11/8); new base . (421/499); total (433/507)
Fisher's exact test for biased strand distribution p-value = 3.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 999 total aligned reads displayed.

ATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAACTTTTCTTATATTTTATTCAGGAGAACTCTTCAAATTTCAACAAATTTCAAAATGTCAGGAAAACCTAAACATTCAACTTGATTTCTAGAACAATTTTTGTGATATAATTTTTCCCAGAAATAAAATAAACTGAGCTTGTCTTCCTAAATATAGGGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAGGAGA  >  AM406671/1921584‑1921890

aTCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAt                                                                                                                                                     >  1:4881163/1‑149 (MQ=255)

   aaGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAtttt                                                                                                                                                  >  1:1451483/1‑149 (MQ=255)

   aaGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAtttt                                                                                                                                                  >  2:7269608/1‑149 (MQ=255)

     gCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAttttcc                                                                                                                                                <  2:5449778/149‑3 (MQ=255)

       aCTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAttttccca                                                                                                                                              >  1:1676936/1‑145 (MQ=255)

         tCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAGa                                                                                                                                           <  2:9093336/149‑1 (MQ=255)

          cGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAGaa                                                                                                                                          >  2:442196/1‑149 (MQ=255)

          cGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAGaa                                                                                                                                          <  1:4500208/149‑1 (MQ=255)

                 ttttACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAg‑aataaaata                                                                                                                                  <  1:4280205/149‑1 (MQ=255)

                    tACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAAc                                                                                                                               >  2:7138712/1‑149 (MQ=255)

                    tACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAAc                                                                                                                               <  1:3982042/149‑1 (MQ=255)

                      caacaaAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTg                                                                                                                             >  1:8300943/1‑149 (MQ=255)

                        acaaAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAg                                                                                                                           <  2:4780574/149‑1 (MQ=255)

                         caaAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGc                                                                                                                          >  1:7073779/1‑149 (MQ=255)

                         caaAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGc                                                                                                                          <  1:6128768/149‑1 (MQ=255)

                            aaaGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGCTCTAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTg                                                                                                                       >  2:5331465/1‑149 (MQ=255)

                             aaGGACTTTTTGACAACATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGt                                                                                                                      <  1:6200912/149‑1 (MQ=255)

                              aGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTc                                                                                                                     >  1:2247601/1‑149 (MQ=255)

                               ggACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTCt                                                                                                                    <  1:7365013/149‑1 (MQ=255)

                                 aCTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTCtcc                                                                                                                  >  1:3627379/1‑147 (MQ=255)

                                 aCTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTCtcc                                                                                                                  <  2:1600093/149‑3 (MQ=255)

                                  cTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTCtcct                                                                                                                 >  1:5977864/1‑146 (MQ=255)

                                    ttttGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTaa                                                                                                              <  1:7224607/149‑1 (MQ=255)

                                     tttGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTaaa                                                                                                             >  1:695662/1‑149 (MQ=255)

                                     tttGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTaaa                                                                                                             <  2:5959401/149‑1 (MQ=255)

                                     tttGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTaaa                                                                                                             >  1:5611198/1‑149 (MQ=255)

                                          cAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAg                                                                                                        <  1:6732096/149‑1 (MQ=255)

                                          cAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAg                                                                                                        >  2:2450640/1‑149 (MQ=255)

                                          cAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAg                                                                                                        <  1:1973758/149‑1 (MQ=255)

                                          cAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAg                                                                                                        >  2:7495274/1‑149 (MQ=255)

                                            atatAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAgga                                                                                                      <  1:30067/149‑2 (MQ=255)

                                            atatAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAgga                                                                                                      >  2:7656673/1‑148 (MQ=255)

                                            atatAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAgga                                                                                                      <  2:3506970/149‑2 (MQ=255)

                                            atatAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAgga                                                                                                      >  1:1846387/1‑148 (MQ=255)

                                             tataAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAggaa                                                                                                     >  1:5423561/1‑147 (MQ=255)

                                             tataAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAggaa                                                                                                     <  2:4000156/149‑3 (MQ=255)

                                               taAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAggaaac                                                                                                   >  1:8426390/1‑145 (MQ=255)

                                               taAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAGGaaac                                                                                                   >  2:1953986/1‑148 (MQ=255)

                                                   actttCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTaa                                                                                               <  1:7765088/147‑1 (MQ=255)

                                                   actttCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTaa                                                                                               <  1:1005215/147‑1 (MQ=255)

                                                    ctttCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTaaa                                                                                              <  2:4651158/148‑1 (MQ=255)

                                                    ctttCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTaaa                                                                                              <  2:8453507/148‑1 (MQ=255)

                                                      ttCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAg                                                                                            <  1:881094/149‑1 (MQ=255)

                                                       tCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGa                                                                                           >  1:2427778/1‑149 (MQ=255)

                                                        cTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGaa                                                                                          <  2:2050008/149‑1 (MQ=255)

                                                         ttATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAAt                                                                                         >  1:1260462/1‑149 (MQ=255)

                                                           atatTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGt                                                                                       <  2:5209232/149‑1 (MQ=255)

                                                                 tttatcagagaACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTAtt                                                                                   >  2:1255682/8‑149 (MQ=255)

                                                                 tttatcagagaACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTC‑CGAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATACAATAAACTGAGCTTTTC‑TCCTAAATATA‑GGAAACTAAAAGAATGCTAtt                                                                                   >  2:4287756/8‑149 (MQ=255)

                                                                  ttatcagagaACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTc                                                                                  >  1:9244928/7‑149 (MQ=255)

                                                                  ttatcagagaACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTc                                                                                  <  1:4611425/143‑1 (MQ=255)

                                                                   tatcagagaACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTcc                                                                                 >  1:6381958/6‑149 (MQ=255)

                                                                   tatcagagaACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTcc                                                                                 >  2:2617833/6‑149 (MQ=255)

                                                                      cagagaACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGAt                                                                              <  2:1796582/147‑1 (MQ=255)

                                                                        gagaACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGAttt                                                                            <  1:5239419/149‑1 (MQ=255)

                                                                        gagaACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGAttt                                                                            >  1:3412410/1‑149 (MQ=255)

                                                                           aaCTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTa                                                                         >  1:5477307/1‑149 (MQ=255)

                                                                                  caaatttc‑acaaa‑ttcaaaaTGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCaga                                                                   <  1:2763891/149‑1 (MQ=255)

                                                                                    aatttc‑acaaa‑ttcaaaaTGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCagaag                                                                 <  1:655550/149‑1 (MQ=255)

                                                                                     atttc‑acaaa‑ttcaaaaTGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCagaaga                                                                <  1:98528/149‑1 (MQ=255)

                                                                                        ttcacaaa‑ttcaaaATGTC‑CGAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑AGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAAc                                                              <  1:3827684/146‑1 (MQ=255)

                                                                                        tgcacaaa‑ttcaaaATGTCA‑GAAAACCT‑AACA‑TCAACTTG‑CTTCTAG‑ACAATTTTTGTGACATAA‑TTTTCCCA‑CAATAAAATAAACTGAGCTTGT‑GTCCTAAACATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAAc                                                              <  2:6622040/146‑1 (MQ=255)

                                                                                          cacaaa‑ttcaaaATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACAt                                                            <  2:8631787/148‑1 (MQ=255)

                                                                                           acaaa‑ttcaaaATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACAtt                                                           >  2:4145108/1‑149 (MQ=255)

                                                                                               aattcaaAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCag                                                                    >  1:7660185/3‑137 (MQ=255)

                                                                                               aattcaaAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACAttttt                                                        <  1:3179247/147‑1 (MQ=255)

                                                                                               aattcaaAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACAttttt                                                        >  2:215893/3‑149 (MQ=255)

                                                                                                attcaaAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACAtttttt                                                       <  2:3768027/148‑1 (MQ=255)

                                                                                                      aaTGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAAttt                                                 >  1:9052930/1‑149 (MQ=255)

                                                                                                      aaTGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAAttt                                                 <  2:6469964/149‑1 (MQ=255)

                                                                                                        tGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCa                                               <  2:8798102/149‑1 (MQ=255)

                                                                                                            cagAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCAttt                                            <  2:3387489/147‑1 (MQ=255)

                                                                                                            cagAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCAttt                                            >  2:7966606/3‑149 (MQ=255)

                                                                                                            cagAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCAttt                                            <  1:7997518/147‑1 (MQ=255)

                                                                                                              gAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCAttttt                                          <  1:7465305/149‑1 (MQ=255)

                                                                                                                aaaCCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCAttttttt                                        >  1:4709286/1‑149 (MQ=255)

                                                                                                                     cctaacatCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑CTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTtata                                     <  2:6347875/142‑1 (MQ=255)

                                                                                                                        aacatCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTa                                  <  2:3133602/145‑1 (MQ=255)

                                                                                                                          catCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAg                                >  1:2625542/3‑149 (MQ=255)

                                                                                                                           atCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAgg                               <  2:5740770/148‑1 (MQ=255)

                                                                                                                            tCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAggg                              <  1:5830358/149‑1 (MQ=255)

                                                                                                                                  ttgattCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAg                         <  2:3657920/145‑1 (MQ=255)

                                                                                                                                      ttCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCa                     >  1:808503/1‑149 (MQ=255)

                                                                                                                                         ctagaCAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCaaa                   <  1:2862344/145‑1 (MQ=255)

                                                                                                                                          tagaCAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTTATTTCATTTTTTTATATTAAGGGATAAGCTCAAAt                  >  2:7799406/4‑149 (MQ=255)

                                                                                                                                          tagaCAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAAt                  <  1:4133672/146‑1 (MQ=255)

                                                                                                                                          tagaCAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATCCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCANTTTTTTATATTAAGGGATAAGCTCAAAt                  <  1:5058577/146‑1 (MQ=255)

                                                                                                                                           agaCAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAAtt                 >  2:5622302/3‑148 (MQ=255)

                                                                                                                                            gaCAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAAtct                >  2:494429/2‑149 (MQ=255)

                                                                                                                                              cAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCa              <  2:8180258/149‑1 (MQ=255)

                                                                                                                                                aTTTTTGTGATATAA‑TTTTCCCAG‑CATAAAATAAACTGAGCTTGAC‑TCCTAATTATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCata            >  1:8631157/1‑149 (MQ=255)

                                                                                                                                                aTTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCata            <  2:8973661/149‑1 (MQ=255)

                                                                                                                                                 tttttGTGATATAA‑GTTTCACAG‑AATAAAATAAACTGCGTTTGTC‑TCCTATATATA‑GGTAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTATTTTCATTTTTTTATATTAAGGGATAAGCCCAAATCTCatct           <  2:6182724/149‑3 (MQ=255)

                                                                                                                                                  ttttGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTATTTTCATTTTTTTATTTTATTGGATAATCTCATATCTCTTAtt          >  2:6799363/1‑149 (MQ=255)

                                                                                                                                                  ttttGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATAtt          <  2:7011525/149‑1 (MQ=255)

                                                                                                                                                  ttttGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATAtt          >  2:2762546/1‑149 (MQ=255)

                                                                                                                                                    ttGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCa        <  1:1097125/149‑1 (MQ=255)

                                                                                                                                                     tgtgATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCaa       >  2:8337753/1‑149 (MQ=255)

                                                                                                                                                      gtgATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAg      <  1:8965500/149‑1 (MQ=255)

                                                                                                                                                       tgATATAA‑TTTTCCCAG‑AATAAAATCAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAgg     >  2:5052068/1‑149 (MQ=255)

ATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAACTTTTCTTATATTTTATTCAGGAGAACTCTTCAAATTTCAACAAATTTCAAAATGTCAGGAAAACCTAAACATTCAACTTGATTTCTAGAACAATTTTTGTGATATAATTTTTCCCAGAAATAAAATAAACTGAGCTTGTCTTCCTAAATATAGGGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCATTTTTTTATATTAAGGGATAAGCTCAAATCTCATATTCAAGGAGA  >  AM406671/1921584‑1921890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`