Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 2,510,293 Δ1 bp coding (777/816 nt) purR ← pur operon repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM4066712,510,2920T.99.1% 5671.7 / 39.8 1222coding (778/816 nt)purRpur operon repressor
Reads supporting (aligned to +/- strand):  ref base T (6/5);  new base . (642/562);  total (654/568)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1265 total aligned reads displayed.

TCAGCGATTATTGGTGAAGCCAAATTACGAGTTTGGCCACTCAATAAAATTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAATTGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCAAGTAAACAATCAAATTCATGAACAAGACTTCTCATTCC  >  AM406671/2510144‑2510436
                                                                                                                                                    |                                                                                                                                                
tCAGCGATTATTGGTGAAGCTAAATTAAGAGTTTGGCCACTCAATAAAATTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTGAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAAt                                                                                                                                                  <  2:8343395/149‑1 (MQ=255)
   gCGATTATTGGTGAAGCCAAATTACGAGTTTGGCCACTCAATAAAATTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAAtgaa                                                                                                                                               >  2:3875751/1‑146 (MQ=255)
    cGATTATTGGTGAAGCCAAATTACGAGTTTGGCCACTCAATAAAATTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAAtgaac                                                                                                                                              >  1:1333862/1‑145 (MQ=255)
    cGATTATTGGTGAAGCCAAATTACGAGTTTGGCCACTCAATAAAATTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAAtgaac                                                                                                                                              <  2:1427918/149‑5 (MQ=255)
     gATTATTGGTGAAGCCAAATTACGAGTTTGGCCACTCAATAAAATTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACg                                                                                                                                            <  1:3966438/149‑1 (MQ=255)
         attGGTGAAGCCAAATTACGAGTTTGGCCACTCAATAAAATTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTg                                                                                                                                        <  1:3728125/149‑1 (MQ=255)
            ggTGAAGCCAAATTACGAGTTTGGCCACTCAATAAAATTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCa                                                                                                                                     <  1:1862658/149‑1 (MQ=255)
              tGAAGCCAAATTACGAGTTTGGCCACTCAATAAAATTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAat                                                                                                                                   <  2:8830424/149‑1 (MQ=255)
              tGAAGCCAAATTACGAGTTTGGCCACTCAATAAAATTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAat                                                                                                                                   <  1:3424764/149‑1 (MQ=255)
              tGAAGCCAAATTACGAGTTTGGCCACTCAATAAAATTTCTGCCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAat                                                                                                                                   <  1:9055750/149‑1 (MQ=255)
                 aGCCAAATTACGAGTTTGGCCACTCAATAAAATTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATc                                                                                                                                >  2:5621920/1‑149 (MQ=255)
                  gCCAAATTACGAGTTTGGCCACTCAATAAAATTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCa                                                                                                                               <  1:7562265/149‑1 (MQ=255)
                  gCCAAATTACGAGATTGTCCACTCAATAAAATATCGTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATATCCCACTTGAACATCAA‑TGAACGATTGCCAATAGCa                                                                                                                               >  2:4420779/1‑149 (MQ=38)
                   ccAAATTACGAGTTTGGCCACTCAATAAAATTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAACTAGATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATcga                                                                                                                              >  2:6649058/1‑147 (MQ=255)
                   ccAAATTACGAGTTTGGCCACTCAATAAAATTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCaa                                                                                                                              <  2:2173265/149‑1 (MQ=255)
                    cAAATTACGAGTTTGGCCACTCAATAAAATTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGAGTGGCAATATCAAt                                                                                                                             >  2:8934912/1‑149 (MQ=255)
                              ttttGGCCACTCAATAAAATTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACt                                                                                                                   <  2:7023607/148‑1 (MQ=255)
                              gTTTGGCCACTCAATAAAATTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGGCAGTAACTTTTTTAGTTGTCACTAAATATATTTCCAAATGGCACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACt                                                                                                                   >  2:3580380/1‑149 (MQ=255)
                                  ggCCACTCAATAAAATTTCTTTCTTTTAAGTAAATAAGCAACAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCa                                                                                                               >  1:4294100/1‑149 (MQ=255)
                                      aCTCAATAAAATTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAgat                                                                                                           >  2:4602402/1‑149 (MQ=255)
                                      aCTCAATAAAATTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAgat                                                                                                           <  2:6272669/149‑1 (MQ=255)
                                       cTCAATAAAATTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAgatt                                                                                                          <  1:2564345/149‑1 (MQ=255)
                                       cTCAAGAAAATTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTTAAAGAATGTCTTTCAGTAACTTTTTTATTTGTCCTTAAATATATTTCCCATTTGAACATCAATT‑AACGATTGGCAATATCAATTCGGTCAACTTTCAAgatt                                                                                                          <  2:8037214/149‑1 (MQ=255)
                                           aTAAACTTTCTTGCTTTTAAGTAAATAAACAAAAAAGATACTGACAGACTGTCAGTCAGTAAGTTTTTTATTTGTCCTTAAATATATTTGACAATTGAACATCTA‑TGACCGATTGGCAATATCAATTCGGTAAACTTTCAGgattgata                                                                                                      >  2:4104359/1‑148 (MQ=255)
                                           aTAAAATTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAgattgatt                                                                                                      >  1:4996037/1‑149 (MQ=255)
                                            tAAAATTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGAttt                                                                                                     >  1:8644765/1‑149 (MQ=255)
                                            tAAAATTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGACTGGCAATATCAATTCGGTCAACTTTCAAGATTGAttt                                                                                                     <  1:6134064/149‑1 (MQ=255)
                                              aaaTTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTtat                                                                                                   <  1:7004670/149‑1 (MQ=255)
                                               aaTTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTtata                                                                                                  >  1:8225308/1‑149 (MQ=255)
                                               aaTTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTtata                                                                                                  >  2:3420407/1‑149 (MQ=255)
                                                aTTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATaa                                                                                                 >  1:5692982/1‑149 (MQ=255)
                                                aTTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATaa                                                                                                 >  1:8413697/1‑149 (MQ=255)
                                                aTTCCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATaa                                                                                                 <  1:2130131/149‑1 (MQ=255)
                                                  ttctttctTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAatc                                                                                               <  1:4162193/149‑1 (MQ=255)
                                                  ttctttctTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAatc                                                                                               <  2:6863219/149‑1 (MQ=255)
                                                      ttctttTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAAtcatca                                                                                           <  2:9241112/149‑1 (MQ=255)
                                                       tctttTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCatcaa                                                                                          <  1:2833512/149‑1 (MQ=255)
                                                        ctttTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCatcaat                                                                                         >  2:7153089/1‑149 (MQ=255)
                                                         ttttAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCatcaatc                                                                                        <  1:7269368/149‑1 (MQ=255)
                                                          tttAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACTATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCatcaatca                                                                                       <  2:1808844/149‑1 (MQ=255)
                                                          tttAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAat                                                                                                >  2:230512/1‑140 (MQ=255)
                                                          tttAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCatcaatca                                                                                       >  1:5188805/1‑149 (MQ=255)
                                                           ttAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCAtcaatcaa                                                                                      <  1:9252723/149‑1 (MQ=255)
                                                            tAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATcaatcaat                                                                                     <  1:8813106/149‑1 (MQ=255)
                                                               gTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGt                                                                                  >  1:1524143/1‑149 (MQ=255)
                                                                tAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGtt                                                                                 >  2:2555580/1‑149 (MQ=255)
                                                                tAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGtt                                                                                 >  2:7468152/1‑149 (MQ=255)
                                                                 aaaTAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAGCGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAACCAATCGTTc                                                                                <  1:4768777/149‑1 (MQ=255)
                                                                 aaaTAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTc                                                                                <  1:4594020/149‑1 (MQ=255)
                                                                  aaTAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCa                                                                               >  1:3240352/1‑149 (MQ=255)
                                                                     aaGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGACTTATAATCATCAATCAATCGTTCAcat                                                                            >  2:9135005/1‑147 (MQ=255)
                                                                      aGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCtt                                                                           <  1:79613/149‑1 (MQ=255)
                                                                      aGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCtt                                                                           >  1:6832607/1‑149 (MQ=255)
                                                                       gCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCttt                                                                          >  2:4838047/1‑149 (MQ=255)
                                                                       gCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCttt                                                                          >  1:2003001/1‑149 (MQ=255)
                                                                        cAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTGCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTa                                                                         <  1:7435485/149‑1 (MQ=255)
                                                                         aaaaaaGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTaa                                                                        <  1:1079090/149‑1 (MQ=255)
                                                                           aaaaGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAAt                                                                      <  2:8681620/149‑1 (MQ=255)
                                                                            aaaGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATg                                                                     <  2:3269355/149‑1 (MQ=255)
                                                                                     aaCAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTCAATGGTCCTTCaa                                                            <  2:5113326/148‑1 (MQ=255)
                                                                                       cAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGa                                                          <  1:6410811/149‑1 (MQ=255)
                                                                                         gAATGTCTGTCAGTAACTTTTTTATTTTTTATTAAATATATTTCCCAATTGAACATCAA‑TGAATGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGaaa                                                        >  2:6425292/1‑149 (MQ=255)
                                                                                          aaTGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGaaaa                                                       <  1:5329854/149‑1 (MQ=255)
                                                                                            tgtctgtcAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCATTCGTTCACCTTTAAATGGTCCTTCAAGaaaaaa                                                     >  2:3742189/1‑148 (MQ=255)
                                                                                                tgtcAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCa                                                 >  1:7652722/1‑149 (MQ=255)
                                                                                                  tcAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAac                                               >  1:1008737/1‑149 (MQ=255)
                                                                                                   cAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAaca                                              <  2:7652656/149‑1 (MQ=255)
                                                                                                   cAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAaca                                              <  1:1225185/149‑1 (MQ=255)
                                                                                                     gTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACAcc                                            >  1:17103/1‑149 (MQ=255)
                                                                                                     gTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACAcc                                            <  2:545376/149‑1 (MQ=255)
                                                                                                      tAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCg                                           <  1:3618139/149‑1 (MQ=255)
                                                                                                        aCTTTTTTTTTTTTTTTTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATATTTATCAATTAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGc                                         >  2:5255070/1‑149 (MQ=255)
                                                                                                        aCTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGc                                         >  1:2392195/1‑149 (MQ=255)
                                                                                                         cTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCa                                        <  1:2700614/149‑1 (MQ=255)
                                                                                                         cTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCa                                        <  1:4450651/149‑1 (MQ=255)
                                                                                                           tttttATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCaaa                                      >  2:4301452/1‑148 (MQ=255)
                                                                                                              ttATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCAAGTaa                                   <  1:3070139/149‑1 (MQ=255)
                                                                                                               tATTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCAAGTaaa                                  <  2:1938614/149‑1 (MQ=255)
                                                                                                                aTTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCAAGTAAAc                                 >  1:8097816/1‑149 (MQ=255)
                                                                                                                aTTTGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGACAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCTTTCAAGAAATATGGCAGCACCGGCAAGTAAAc                                 >  2:1687400/1‑149 (MQ=255)
                                                                                                                 tttGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCAAGTAAACa                                >  1:228648/1‑149 (MQ=255)
                                                                                                                 tttGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCAAGTAAACa                                <  1:6008268/149‑1 (MQ=255)
                                                                                                                 tttGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTCTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCAAGTAAACa                                >  1:5848606/1‑149 (MQ=255)
                                                                                                                 tttGTCATTAAATATATTTACCAATAGAACATCAATT‑AACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGAATTCTAATCAACAATCAATCGTTCGCCTTTAAATGGTCCTTCAAAAATAACGGCAACACCGCCAAGTAAACa                                <  2:2917945/149‑1 (MQ=255)
                                                                                                                   tGTCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCAAGTAAACAAt                              >  2:1168881/1‑149 (MQ=255)
                                                                                                                     tCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCTATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCAAGTAAACAATCa                            <  1:5524497/149‑1 (MQ=255)
                                                                                                                     tCATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCAAGTAAACAATCa                            >  2:3143526/1‑149 (MQ=255)
                                                                                                                      cATTAAATATATTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCAAGTAAACAATCaa                           >  1:6271943/1‑149 (MQ=255)
                                                                                                                            atatatTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCAAGTAAACAATCAAATTCAt                     <  2:8411177/149‑1 (MQ=255)
                                                                                                                             tatatTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCAAGTAAACAATCAAATTCATg                    >  2:2733310/1‑149 (MQ=255)
                                                                                                                                atTTCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCAAGTAAACAATCAAATTCATGAAc                 <  2:4723220/149‑1 (MQ=255)
                                                                                                                                 tttCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCAAGTAAACAATCAAATTCATGAACa                >  1:7785928/1‑149 (MQ=255)
                                                                                                                                   tCCCAATTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCAAGTAAACAATCAAATTCATGAACaaa              >  2:1089917/1‑148 (MQ=255)
                                                                                                                                     ccAATTGAACATCAA‑TGAACGACTGGCAATATCAATTCGGTCAACTTTCAAGATCAATTTATAATCATCCATAAATCGCTCACCTTTAAATGGTCCTTCAAGAAACAGGGAAAAACCGACAAGTAAACACTCAAACTAATGAACAAgcc            >  2:7792354/1‑147 (MQ=255)
                                                                                                                                       aaTTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCAAGTAAACAATCAAATTCATGAACAAGACtt          >  2:5893519/1‑149 (MQ=255)
                                                                                                                                        aTTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCAAGTAAACAATCAAATTCATGAACAAGACTtc         <  2:1281857/149‑1 (MQ=255)
                                                                                                                                        aTTGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCAAGTAAACAATCAAATTCATGAACAAGACTtc         >  2:6091663/1‑149 (MQ=255)
                                                                                                                                          tGAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCAAGTAAACAATCAAATTCATGAACAAGACTtctc       <  1:7119468/149‑1 (MQ=255)
                                                                                                                                           gAACATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACCTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCAAGTAAACAATCAAATTCATGAACAAGACTTCTCa      <  1:6674242/149‑1 (MQ=255)
                                                                                                                                             aCATCAA‑TGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGGTTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCAAGTAAACAATCAAATTCATGAACAAGACTTCTCAtt    >  1:8532747/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TCAGCGATTATTGGTGAAGCCAAATTACGAGTTTGGCCACTCAATAAAATTTCTTTCTTTTAAGTAAATAAGCAAAAAAGTTACTGACAGAATGTCTGTCAGTAACTTTTTTATTTGTCATTAAATATATTTCCCAATTGAACATCAATTGAACGATTGGCAATATCAATTCGGTCAACTTTCAAGATTGATTTATAATCATCAATCAATCGTTCACCTTTAAATGGTCCTTCAAGAAAAACGGCAACACCGGCAAGTAAACAATCAAATTCATGAACAAGACTTCTCATTCC  >  AM406671/2510144‑2510436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑