BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	945,239	+C	pseudogene (229/761 nt)	tnp904 →	pseudogene

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	945,239	1	.	C	99.8%	1018.8 / NA	441	pseudogene (229/761 nt)	tnp904	pseudogene
Reads supporting (aligned to +/- strand): ref base . (1/0); major base C (227/212); minor base A (1/0); total (229/212)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 475 total aligned reads displayed.

GGGACGCTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑G‑TTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGCTGGGAGACATGACCTATATCCCTACCAAAGAAGGCACCTTATACTTA  >  AM406671/945091‑945351

gggACGCTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑g                                                                                                                   >  1:7842432/1‑149 (MQ=38)

  gACGCTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑gct                                                                                                                 <  1:7337079/149‑3 (MQ=37)

    cGCTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑gcttc                                                                                                               <  2:3592802/149‑5 (MQ=25)

     gCTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑gcttcg                                                                                                              >  1:1901225/1‑144 (MQ=25)

      cTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGc                                                                                                             >  1:1723324/1‑149 (MQ=21)

       tATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACCCGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCt                                                                                                            >  1:1049676/1‑149 (MQ=12)

        aTGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCATGGGAAGCCGTTATAAATATAGAGATTATAACAGAAAAGGA‑GCTTCGCtt                                                                                                           >  2:1990275/1‑149 (MQ=11)

        aTGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCtt                                                                                                           <  1:4177609/149‑1 (MQ=21)

        aTGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCtt                                                                                                           <  1:2952194/149‑1 (MQ=21)

        aTGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCtt                                                                                                           <  1:769195/149‑1 (MQ=21)

         tGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCttt                                                                                                          <  1:456346/149‑1 (MQ=17)

         tGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCttt                                                                                                          >  2:6568752/1‑149 (MQ=18)

         tGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCttt                                                                                                          <  2:5877165/149‑1 (MQ=18)

         gggTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCttt                                                                                                          <  1:1569981/148‑1 (MQ=21)

          ggTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTc                                                                                                         >  1:5962296/1‑149 (MQ=21)

           gTGCGGTTAGAATTACCAAGGTACTTCATAATACTTGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCa                                                                                                        <  1:3681116/149‑1 (MQ=12)

           gTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCa                                                                                                        <  2:4122953/149‑1 (MQ=17)

            tGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCaa                                                                                                       >  1:4821762/1‑149 (MQ=21)

             gCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAg                                                                                                      <  2:8840155/149‑1 (MQ=21)

               ggTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTAGATAAGACTTTATGCCAAGGGAAGCCCTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGAc                                                                                                    <  2:5518903/149‑1 (MQ=11)

                gTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGAcc                                                                                                   >  1:7225/1‑149 (MQ=21)

                 ttAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGAccc                                                                                                  <  2:7705338/149‑1 (MQ=21)

                 ttAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGAccc                                                                                                  >  2:7884131/1‑149 (MQ=21)

                 ttAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGAccc                                                                                                  >  1:5574783/1‑149 (MQ=21)

                  tAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCa                                                                                                 <  1:8153621/149‑1 (MQ=18)

                     aaTTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAAtt                                                                                              <  2:3334588/149‑1 (MQ=21)

                     aaTTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAAtt                                                                                              >  2:1564781/1‑149 (MQ=17)

                      aTTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGAAGTTATGCCAAGGGGCGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGCCCCAAtct                                                                                             >  2:2239143/1‑147 (MQ=11)

                       ttACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATtta                                                                                            >  1:7599933/1‑149 (MQ=18)

                        tACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATttaa                                                                                           >  2:4820553/1‑149 (MQ=21)

                        tACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATttaa                                                                                           <  1:6591838/149‑1 (MQ=21)

                         aCCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATttaat                                                                                          <  1:5348528/149‑1 (MQ=21)

                           cAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATttaatta                                                                                        >  1:8837863/1‑149 (MQ=21)

                            aaGGTACTTCATAATACTGGTATTATAACCAACACGCAACGTGTGGGGAAACTGATGCACTTGATGGGCCTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATttaattaa                                                                                       >  1:8765658/1‑149 (MQ=255)

                            aaGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATttaattaa                                                                                       <  2:7011119/149‑1 (MQ=18)

                             aGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTtaattaat                                                                                      <  2:1708816/149‑1 (MQ=21)

                             aGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATCtaattaat                                                                                      >  2:3456634/1‑149 (MQ=255)

                              ggTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGAccc                                                                                                  <  1:7918634/136‑1 (MQ=21)

                              ggTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATc                                                                                     <  1:966330/149‑1 (MQ=18)

                               gTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCa                                                                                    >  1:8043351/1‑149 (MQ=21)

                                tACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAg                                                                                   <  1:7035631/149‑1 (MQ=21)

                                tACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAg                                                                                   <  2:2316071/149‑1 (MQ=21)

                                tACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAg                                                                                   >  1:8265981/1‑149 (MQ=21)

                                 aCTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGa                                                                                  <  2:8629366/149‑1 (MQ=21)

                                  cTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGAt                                                                                 <  1:6387498/149‑1 (MQ=21)

                                  cTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGAt                                                                                 >  2:3018095/1‑149 (MQ=17)

                                  cTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGAt                                                                                 <  1:2528214/149‑1 (MQ=21)

                                   ttCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATc                                                                                >  2:4332625/1‑149 (MQ=18)

                                   atCATAATACTGGTATTATAACCACCACGTAACGTGTGGGGAAACTGAAGCACTTGATGGAGCTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACCGAACAGGACC‑TTTGCTTTAAACACCCACTTTACTTAATCAGCTc                                                                                >  2:5984430/2‑149 (MQ=11)

                                    tCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCt                                                                               <  1:1299683/149‑1 (MQ=17)

                                     cATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCtt                                                                              >  2:8516580/1‑149 (MQ=18)

                                      ataataCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCttt                                                                             <  2:2842585/149‑1 (MQ=18)

                                      ataataATGGTATTATAAACAACACGAAACGTGGGGGGAAACTGATGCACTTGATGGGACTTTATGGCAAGGGAAGCCGTTATAAATATACACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCttt                                                                             >  2:4493634/1‑149 (MQ=11)

                                       taataCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTa                                                                            <  2:7254491/149‑1 (MQ=21)

                                       taataCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTa                                                                            <  2:5072336/149‑1 (MQ=17)

                                       taataCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTa                                                                            >  1:226100/1‑149 (MQ=21)

                                        aataCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTaa                                                                           <  1:7453537/149‑1 (MQ=21)

                                        aataCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTaa                                                                           >  1:3466376/1‑149 (MQ=21)

                                        aataCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTAGATGGGACTGTATGCGAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGTCCCAATTTAATTAATCCGATCTTTaa                                                                           <  2:8945278/149‑1 (MQ=11)

                                         ataCTGGTATTATAACCAACACGAAACGTGTGGGGAAAGTGTTTCACTTGATGGGACTATATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTaaa                                                                          >  2:4645524/1‑149 (MQ=11)

                                         ataCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTaaa                                                                          <  2:2630294/149‑1 (MQ=17)

                                          taCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGTCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAg                                                                         >  2:8689759/1‑149 (MQ=16)

                                          taCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAg                                                                         >  2:3907828/1‑149 (MQ=21)

                                           aCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGc                                                                        <  1:915029/149‑1 (MQ=18)

                                           aCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGc                                                                        >  1:329906/1‑149 (MQ=21)

                                            cTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCa                                                                       >  1:1219988/1‑149 (MQ=21)

                                            cTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCa                                                                       >  1:1836055/1‑149 (MQ=21)

                                             tGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCaa                                                                      <  2:4186186/149‑1 (MQ=21)

                                             tGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCaa                                                                      <  1:4517110/149‑1 (MQ=21)

                                             tGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATAAGATCTTTAAAGCaa                                                                      >  2:1830654/1‑149 (MQ=12)

                                              ggTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAAc                                                                     >  1:6818171/1‑149 (MQ=21)

                                              ggTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAAc                                                                     >  1:3079294/1‑149 (MQ=18)

                                               gTATTATAACCAACACGATACGTGTGGGGTAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCACTTTAATTAATCAGATCTTTAAAGCAACa                                                                    <  2:5374734/149‑1 (MQ=11)

                                               gTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTAGAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACa                                                                    >  2:5745014/1‑149 (MQ=255)

                                                tattatAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAg                                                                   <  2:9133677/149‑1 (MQ=21)

                                                 attatAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCCACAGc                                                                  <  2:797027/149‑1 (MQ=16)

                                                 attatAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGc                                                                  >  1:3848897/1‑149 (MQ=18)

                                                   tataACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTc                                                                >  1:4361486/1‑149 (MQ=21)

                                                   tataACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTc                                                                <  2:6708538/149‑1 (MQ=17)

                                                      aaCCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCta                                                             >  1:8212756/1‑149 (MQ=14)

                                                        ccAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCtaat                                                           >  1:2042508/1‑149 (MQ=11)

                                                         cAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATTACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCtaata                                                          <  1:5763383/149‑1 (MQ=2)

                                                         cAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCtaata                                                          >  2:8321420/1‑149 (MQ=14)

                                                          aaCACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCtaataa                                                         >  1:218895/1‑149 (MQ=1)

                                                          aaCACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCtaataa                                                         >  2:754157/1‑149 (MQ=14)

                                                            cacGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAg                                                       >  2:2508942/1‑149 (MQ=14)

                                                              cGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCATATCTTTAAAGCAACAGCTCCTAATAAAGTa                                                     <  2:112130/149‑1 (MQ=0)

                                                               gAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTAt                                                    >  1:6902644/1‑149 (MQ=11)

                                                               gAAACGTGTGATGACACTAATGCACTTGACTGGACTTTATGCCAAGGGAAGCCGTTATAAATATACTCATCCTAACAGAAAAGGA‑GCTTCGCTCGCGAGTCCCCATTTAACTAATCAGCTCTTGAAAGCAACACCTCCTAATAGAGtct                                                    <  2:4966042/149‑3 (MQ=0)

                                                                aaaCGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATg                                                   >  1:50159/1‑149 (MQ=11)

                                                                 aaCGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATgg                                                  >  2:6847648/1‑149 (MQ=14)

                                                                 aaCGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATgg                                                  >  2:3761350/1‑149 (MQ=14)

                                                                 aaCGTGAGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGAACTTTAAAGCAACAGCTCCTAATAAAGTATgg                                                  >  2:824596/1‑149 (MQ=2)

                                                                  aCGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGc                                                 >  2:8103074/1‑149 (MQ=11)

                                                                  aCGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGc                                                 <  1:2775512/149‑1 (MQ=11)

                                                                  aCGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATAGAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGc                                                 >  1:5390539/1‑149 (MQ=9)

                                                                   cGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGCt                                                >  2:6293108/1‑149 (MQ=11)

                                                                    gtgtGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGCTg                                               <  1:4636006/149‑1 (MQ=14)

                                                                    gtgtGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGCTg                                               <  2:927735/149‑1 (MQ=11)

                                                                     tgtgGGGAAACTGATGCACTTGATGGGACTTTATGCAAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGCTgg                                              >  2:6154163/1‑149 (MQ=9)

GGGACGCTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑G‑TTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGCTGGGAGACATGACCTATATCCCTACCAAAGAAGGCACCTTATACTTA  >  AM406671/945091‑945351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`