BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,614,130	+T	coding (1149/1278 nt)	mleP ←	malate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,614,128	1	.	T	98.7%	3201.2 / 21.3	835	coding (1151/1278 nt)	mleP	malate transporter
Reads supporting (aligned to +/- strand): ref base . (3/8); new base T (418/404); total (422/413)
Fisher's exact test for biased strand distribution p-value = 1.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 885 total aligned reads displayed.

TTTTTTTAGGTTATGGTTATTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑G‑TTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGCCATGTAAAT  >  AM406671/1613980‑1614271

tttttttAGGTTATGGTTATTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑g                                                                                                                                                  <  1:4228218/149‑1 (MQ=255)

ttttttgAGGTTAAGCCTAATTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGCACAATTGCACCACAGAGTCTATTAGCCAGCTGAGCAAAAGCCATCATTTCCATTCGATCTGATGCAGACAAGGCAGCAATGTTACCT‑g                                                                                                                                                  <  2:2767058/149‑1 (MQ=255)

 ttttttAGTTTATGGTTATTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑G‑t                                                                                                                                                >  2:205477/1‑149 (MQ=255)

 ttttttAGGTTATGGTTATTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑G‑t                                                                                                                                                <  1:5667402/149‑1 (MQ=255)

      tAGGTTATGGTTATTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑G‑tttcca                                                                                                                                           <  1:804841/149‑5 (MQ=255)

           tatggttatTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCAt                                                                                                                                       >  1:5478801/1‑149 (MQ=255)

           tatggttatTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCAt                                                                                                                                       >  1:8726771/1‑149 (MQ=255)

           tatggttatTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCAt                                                                                                                                       >  1:3131184/1‑149 (MQ=255)

            atggttatTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATa                                                                                                                                      >  1:5301512/1‑149 (MQ=255)

            atggtcatTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATa                                                                                                                                      <  2:2979404/149‑1 (MQ=255)

             tggttatTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGAACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAg                                                                                                                                     <  1:3711691/149‑1 (MQ=255)

              ggttatTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGa                                                                                                                                    >  1:4589544/1‑149 (MQ=255)

              ggttatTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATATGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGa                                                                                                                                    >  2:1005527/1‑149 (MQ=255)

                ttatTTTAATAAAAGAATCGTATAAGAATCCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCAATTCGATCTGATGCAGACAAGACAGCAATGTTACAT‑GTTTCCAACCATAGAat                                                                                                                                  >  2:5664585/1‑149 (MQ=255)

                  atTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAatta                                                                                                                                <  1:4465027/149‑1 (MQ=255)

                      taataaAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGa                                                                                                                            <  2:1936817/149‑1 (MQ=255)

                       aataaAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGAt                                                                                                                           >  2:7324919/1‑149 (MQ=255)

                         taaAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCa                                                                                                                         >  2:8161651/1‑149 (MQ=255)

                           aaaGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATa                                                                                                                       >  2:3356470/1‑149 (MQ=255)

                            aaGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATAc                                                                                                                      >  1:788166/1‑149 (MQ=255)

                            aaGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATAc                                                                                                                      <  1:4920133/149‑1 (MQ=255)

                             aGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATAcc                                                                                                                     >  1:624210/1‑149 (MQ=255)

                              gAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATAATAcca                                                                                                                    >  2:4641500/1‑148 (MQ=255)

                                aTCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGc                                                                                                                  <  1:7236726/149‑1 (MQ=255)

                                 tCGTATAAGAATTCCACCGAATATGCGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGcc                                                                                                                 >  2:4992181/1‑148 (MQ=255)

                                 tCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCa                                                                                                                 >  1:1298460/1‑149 (MQ=255)

                                 tCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCa                                                                                                                 >  2:3834763/1‑149 (MQ=255)

                                 tCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCa                                                                                                                 >  1:146260/1‑149 (MQ=255)

                                     ataAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCaa                                                                                                             <  1:676289/149‑1 (MQ=255)

                                      taAGACTTCCACCGAATATGAGGACAATTGCACCACAGCGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACACCAATGTTACCT‑GTTTCCACCCATAGAATTATTCATCATACCTGCACCAAt                                                                                                            >  2:8511648/1‑149 (MQ=255)

                                       aaGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATCTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATg                                                                                                           >  2:5789489/1‑149 (MQ=255)

                                        aGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATgg                                                                                                          <  1:1688674/149‑1 (MQ=255)

                                        aGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATgg                                                                                                          <  2:1344854/149‑1 (MQ=255)

                                          aaTTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCa                                                                                                        <  1:6907050/149‑1 (MQ=255)

                                          aaTTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCa                                                                                                        <  1:262349/149‑1 (MQ=255)

                                           aTTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAg                                                                                                       <  1:4378599/149‑1 (MQ=255)

                                           aTTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAg                                                                                                       >  1:6470195/1‑149 (MQ=255)

                                            ttCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGt                                                                                                      >  1:8516349/1‑149 (MQ=255)

                                            ttCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGt                                                                                                      <  2:7718033/149‑1 (MQ=255)

                                              ccACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTTATCATACCTGCACCAATGGCAGttt                                                                                                    <  2:3113530/149‑1 (MQ=255)

                                                  cGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGAc                                                                                                <  2:8045339/149‑1 (MQ=255)

                                                     atatGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTgg                                                                                             <  1:6153250/149‑1 (MQ=255)

                                                      tatGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGa                                                                                            <  2:4281864/149‑1 (MQ=255)

                                                       atGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGAt                                                                                           >  2:2477455/1‑149 (MQ=255)

                                                        tGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATa                                                                                          >  1:3528186/1‑149 (MQ=255)

                                                               aaTTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCa                                                                                   >  1:6593925/1‑149 (MQ=255)

                                                                  tGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAag                                                                                >  2:9216189/1‑149 (MQ=255)

                                                                  tGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAag                                                                                <  1:2931949/149‑1 (MQ=255)

                                                                    caccacAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAagag                                                                              <  1:1623754/149‑1 (MQ=255)

                                                                    caccacAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAagag                                                                              <  1:5563405/149‑1 (MQ=255)

                                                                           gagTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGcc                                                                       <  1:9009703/149‑1 (MQ=255)

                                                                            agTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCa                                                                      <  1:212167/149‑1 (MQ=255)

                                                                             gTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCaa                                                                     <  1:7086226/149‑1 (MQ=255)

                                                                              tCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGANTGGATAAAGGCCAAAGAGGCGAGCCAAg                                                                    <  1:5102283/149‑1 (MQ=255)

                                                                              tCTATGAGCCATATGAGAAAAAGCAAGCATTTCCATTCGAGCTGATGCAGAGAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAACGATTGATCATACCTGCACCAATGGAAGTTTCGTCTGGATAAAGGCCAAAGACGCGAGCCAAg                                                                    <  2:5421700/149‑1 (MQ=255)

                                                                                tATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGaa                                                                  >  2:743930/1‑149 (MQ=255)

                                                                                  ttAGCCAGCTGAGCAAAAGCAAGCATTTCCATTCGAGCTGATGCAGACCAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATCAAGGCCAAAGAGGCGAGCCAAGAAcc                                                                <  2:1750239/149‑1 (MQ=255)

                                                                                   tAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCa                                                               <  1:7594336/149‑1 (MQ=255)

                                                                                    aGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCaa                                                              >  2:9087039/1‑149 (MQ=255)

                                                                                    aGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCaa                                                              <  2:1184830/149‑1 (MQ=255)

                                                                                     gCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAAc                                                             <  2:3994648/149‑1 (MQ=255)

                                                                                       cATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTa                                                           <  2:3582744/149‑1 (MQ=255)

                                                                                          cTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATAACTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCa                                                        >  1:2458983/1‑149 (MQ=255)

                                                                                           tGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCaa                                                       <  1:2207376/149‑1 (MQ=255)

                                                                                            gAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAg                                                      <  1:7763363/149‑1 (MQ=255)

                                                                                             aGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGt                                                     <  2:6068571/149‑1 (MQ=255)

                                                                                                aaaaGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTAGATAAAGACCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTccc                                                  >  2:6181212/1‑149 (MQ=255)

                                                                                                 aaaGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCa                                                 >  2:673502/1‑149 (MQ=255)

                                                                                                  aaGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCAt                                                >  2:2278801/1‑149 (MQ=255)

                                                                                                  aaGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCAt                                                <  1:276574/149‑1 (MQ=255)

                                                                                                       aTCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTacaa                                           <  2:8709846/149‑1 (MQ=255)

                                                                                                        tCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTacaac                                          >  2:7200495/1‑149 (MQ=255)

                                                                                                         cATTTCCAGTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACACATAGAATTGTTGATCATACCTGCACCAATGGCAGTTCCGACTAGATAAAGGCCAAAGAGGCGAGCCAAAAACCAACTAGCAAGTCCCATTacaaca                                         <  2:6464859/149‑1 (MQ=255)

                                                                                                            ttCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTacaacaaca                                      >  2:3921706/1‑149 (MQ=255)

                                                                                                             tCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAacac                                     <  1:1034649/149‑1 (MQ=255)

                                                                                                               cATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACtt                                   >  1:1154702/1‑149 (MQ=255)

                                                                                                                aTTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTg                                  >  2:6579592/1‑149 (MQ=255)

                                                                                                                  tCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTc                                <  2:1634107/149‑1 (MQ=255)

                                                                                                                  tCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTc                                <  2:7817648/149‑1 (MQ=255)

                                                                                                                   cGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCa                               <  2:5281613/149‑1 (MQ=255)

                                                                                                                   cGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCa                               >  2:7576001/1‑149 (MQ=255)

                                                                                                                       cTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCa                           >  1:188457/1‑149 (MQ=255)

                                                                                                                          aTGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAg                        >  2:2602227/1‑149 (MQ=255)

                                                                                                                             cAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGTCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTac                     >  2:5095635/1‑149 (MQ=255)

                                                                                                                              agacaagacaGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTaca                    >  2:4534642/1‑149 (MQ=255)

                                                                                                                              agacaagacaGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTaca                    >  1:6334987/1‑149 (MQ=255)

                                                                                                                               gacaagacaGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTacaa                   >  2:8320360/1‑149 (MQ=255)

                                                                                                                               gaaaagacaGCCATGTTACCTCT‑TTCCACCCATAGAATTATTGAGCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTacaa                   <  2:5899030/146‑1 (MQ=255)

                                                                                                                                 caagacaGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTacaaca                 >  1:5999419/1‑149 (MQ=255)

                                                                                                                                  aagacaGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCGATTACAACAACACTTGTCAAGCAAAGTAcaacaa                >  2:8907800/1‑149 (MQ=255)

                                                                                                                                    gacaGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAAt              <  1:3374903/149‑1 (MQ=255)

                                                                                                                                     acaGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAAtt             >  1:815950/1‑149 (MQ=255)

                                                                                                                                     acaGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAAtt             >  2:4794766/1‑149 (MQ=255)

                                                                                                                                       aGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGc           <  2:7004951/149‑1 (MQ=255)

                                                                                                                                       aGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGc           <  1:4776364/149‑1 (MQ=255)

                                                                                                                                        gCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGcc          >  2:4387270/1‑149 (MQ=255)

                                                                                                                                        gCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGcc          <  1:7421079/149‑1 (MQ=255)

                                                                                                                                          aaTGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGCCAt        <  1:3967095/149‑1 (MQ=255)

                                                                                                                                            tGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGCCATGt      >  1:9108597/1‑149 (MQ=255)

                                                                                                                                               tACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGCCATGTaaa   >  1:3036461/1‑149 (MQ=255)

TTTTTTTAGGTTATGGTTATTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑G‑TTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGCCATGTAAAT  >  AM406671/1613980‑1614271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`