Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 827,811 C→T intergenic (+153/‑32) uxuA → / → uxuT D‑mannonate hydrolase/Na‑galactoside symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM406671827,8110CT98.9% 3441.2 / NA 917intergenic (+153/‑32)uxuA/uxuTD‑mannonate hydrolase/Na‑galactoside symporter
Reads supporting (aligned to +/- strand):  ref base C (0/2);  major base T (496/406);  minor base A (0/10);  total (497/420)
Fisher's exact test for biased strand distribution p-value = 3.67e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 937 total aligned reads displayed.

GTTTTATGAAGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGACAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACAATATTGTT  >  AM406671/827665‑827956
                                                                                                                                                  |                                                                                                                                                 
  tttATGAAGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATaaaa                                                                                                                                               <  1:4477598/149‑1 (MQ=255)
   ttATGAAGGAATCTATTTTAAAGAACTTTTTTTTTTTTTTTTTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACATAGTTCACTATTTAACTAAGTTTATGGAAAAATTTGTCAATTTTTTTTGATaaaaa                                                                                                                                              >  2:4595205/1‑149 (MQ=255)
   ttATGAAGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATaaaaa                                                                                                                                              <  1:3780133/149‑1 (MQ=255)
      tGAAGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAatat                                                                                                                                           <  1:8405460/149‑1 (MQ=255)
        aaGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATaa                                                                                                                                         >  1:5650614/1‑149 (MQ=255)
        aaGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATaa                                                                                                                                         <  1:6789624/149‑1 (MQ=255)
           gAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATaaaaa                                                                                                                                      <  1:1224229/149‑1 (MQ=255)
           gAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATaaaaa                                                                                                                                      >  1:5179114/1‑149 (MQ=255)
            aaTCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGATAAATTAAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGAAAAAAAAAAAAAAAt                                                                                                                                     <  2:4254447/149‑1 (MQ=255)
              tCTATTTTAAAGAACTTTTTTTTTTTTTTTGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAg                                                                                                                                   >  2:8064775/1‑149 (MQ=255)
                tATTTTAAAGAACTTATTGTAAAAAAATGTGCGGACACTATTCTCAGCAAAAATTAGAACGACGGAGTAAGCGTTTCACAAATTTCACAATATAACAAAGTTTCTAGAAAAATTTGTCAATTTTCATTGAAAAAAAAAAAAAAATAGga                                                                                                                                 <  2:6660534/149‑1 (MQ=255)
                 aTTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGgag                                                                                                                                <  1:2721722/149‑1 (MQ=255)
                     tAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATc                                                                                                                            <  1:1012488/149‑1 (MQ=255)
                      aaaGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCt                                                                                                                           >  1:7036596/1‑149 (MQ=255)
                       aaGAACTTTTTGTGAAAAAATGTGAGAAAAAAATTAACCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAACTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGAAAAAAAAAAAAAAATAGGAGAATCTg                                                                                                                          <  2:5760267/149‑1 (MQ=255)
                        aGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGt                                                                                                                         <  1:8822507/149‑1 (MQ=255)
                        aGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTATAAAAAAAAAAAATATAAAAATAGGAGAATCTGt                                                                                                                         <  2:4101365/149‑1 (MQ=255)
                         gAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGtt                                                                                                                        <  2:3018009/149‑1 (MQ=255)
                         gAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGtt                                                                                                                        >  2:4910026/1‑149 (MQ=255)
                         gAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGtt                                                                                                                        <  2:3343390/149‑1 (MQ=255)
                         gAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGtt                                                                                                                        <  2:8580606/149‑1 (MQ=255)
                          aaCTTTTTGTGAAAAAATGTGCTGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAATTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGATAATCTGttt                                                                                                                       <  2:6794002/149‑1 (MQ=255)
                              ttttGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTaaaa                                                                                                                   <  2:3166262/149‑1 (MQ=255)
                              ttttGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTaaaa                                                                                                                   >  1:755923/1‑149 (MQ=255)
                               tttGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAAt                                                                                                                  >  1:2979337/1‑149 (MQ=255)
                                   tgAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCa                                                                                                              <  2:1302122/149‑1 (MQ=255)
                                    gAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGCTCACAATTTAACAAAGTTTATGGAAAAACTTGTCAATCTTCACTGCTAAAAATATACAAATAGGAGAATCTGTTTAAAATGGCAg                                                                                                             >  2:1069291/1‑149 (MQ=255)
                                       aaaaTGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGaaa                                                                                                          <  1:2875025/149‑1 (MQ=255)
                                       aaaaTGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGaaa                                                                                                          <  2:6035886/149‑1 (MQ=255)
                                       aaaaTGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGaaa                                                                                                          <  2:8460007/149‑1 (MQ=255)
                                        aaaTGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGaaaa                                                                                                         >  1:2567127/1‑149 (MQ=255)
                                        aaaTGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGaaaa                                                                                                         >  2:625600/1‑149 (MQ=255)
                                         aaTGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGaaaaa                                                                                                        <  2:250987/149‑1 (MQ=255)
                                          aTGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAAc                                                                                                       >  1:6367244/1‑149 (MQ=255)
                                          aTGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTATATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAACATGGCAGaacaac                                                                                                       >  2:2249832/1‑149 (MQ=255)
                                             tgCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGGGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACaaa                                                                                                    <  1:4619634/149‑1 (MQ=255)
                                             tgCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACaaa                                                                                                    >  2:4419137/1‑149 (MQ=255)
                                             tgCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACaaa                                                                                                    >  1:6626113/1‑149 (MQ=255)
                                             tgCGGAGACTTTTCACCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAAATAAAAAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGaaaaaaaaaaaa                                                                                                    <  2:8209989/149‑1 (MQ=255)
                                              gCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACaaaa                                                                                                   <  1:1539046/149‑1 (MQ=255)
                                                ggAGACATATCACCGCTAAATTTAGAACGACGGAGTAAGCATATCACAAAGAACACAATTAAACAAAGTATATAAAAAAATTTGTCAATTTTCACTAATAAAAATATAAAAATAGGAGAATCTGTTTAAAAAAAAAAAAAAACaaaaaa                                                                                                 <  2:5321496/149‑1 (MQ=255)
                                                 gagaCTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAAc                                                                                                >  2:6439099/1‑149 (MQ=255)
                                                  agaCTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACt                                                                                               <  1:1859775/149‑1 (MQ=255)
                                                    aCTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTc                                                                                             >  1:8166255/1‑149 (MQ=255)
                                                         tctcCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTggg                                                                                        <  1:4194366/149‑1 (MQ=255)
                                                            ccGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGttt                                                                                     >  2:8975086/1‑149 (MQ=255)
                                                                 aaaTTTAGAACGACGGCGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTacaca                                                                                <  1:6190074/149‑1 (MQ=255)
                                                                 aaaTTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTacaca                                                                                >  2:5179443/1‑149 (MQ=255)
                                                                 aaaTTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTacaca                                                                                >  1:3629760/1‑149 (MQ=255)
                                                                   aTTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACAcc                                                                              >  1:7746927/1‑149 (MQ=255)
                                                                   aTTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACAcc                                                                              <  2:7556182/149‑1 (MQ=255)
                                                                        gAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAg                                                                         >  2:9079774/1‑149 (MQ=255)
                                                                          acgacgGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGaa                                                                       >  1:3189862/1‑149 (MQ=255)
                                                                           cgacgGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGaaa                                                                      <  1:7333421/149‑1 (MQ=255)
                                                                            gacgGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGCATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAAt                                                                     >  1:6091083/1‑149 (MQ=255)
                                                                             acgGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAAtt                                                                    >  2:3704661/1‑149 (MQ=255)
                                                                              cgGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAACATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTa                                                                   >  2:5410259/1‑149 (MQ=255)
                                                                              cgGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTa                                                                   >  2:6566054/1‑149 (MQ=255)
                                                                               ggAATAAACGTATCACAAAGGTAACAAAATAACAAAGTTTATGCAAAAAATTGTCAATTTTAACAGAAAAAAAAATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTaa                                                                  <  2:3935743/149‑1 (MQ=255)
                                                                                 aGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATa                                                                >  2:5587269/1‑149 (MQ=255)
                                                                                    aaGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACaa                                                             >  2:1826642/1‑149 (MQ=255)
                                                                                    aaGAGTTTCAAAAAGTTCACAATATAACAAAGTTTATGGAAAAATATGTCAATTATAACTGATAAAAATATAAAAATAAGAGAAAATGTAAAAAAAAAAAAAAAAAAAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACaa                                                             <  2:5920112/149‑1 (MQ=255)
                                                                                     aGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAg                                                            <  2:6922142/149‑1 (MQ=255)
                                                                                         tttCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAACAGAAATTAATACAAggtgg                                                        >  2:4738677/1‑146 (MQ=255)
                                                                                          ttCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTg                                                       >  1:3549573/1‑149 (MQ=255)
                                                                                          ttCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTg                                                       >  2:1472701/1‑149 (MQ=255)
                                                                                           tCACAAAGTTCACAATTTAATAAAGTATATGAAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTgg                                                      <  2:2022885/149‑1 (MQ=255)
                                                                                           tCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTgg                                                      >  1:2399594/1‑149 (MQ=255)
                                                                                             acaAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAgg                                                           <  1:6873311/142‑1 (MQ=255)
                                                                                             acaAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGtc                                                    <  1:9100828/149‑1 (MQ=255)
                                                                                                aaGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGtcttc                                                 >  1:65147/1‑149 (MQ=255)
                                                                                                 aGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGtcttct                                                >  1:545952/1‑149 (MQ=255)
                                                                                                 aGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGtcttct                                                >  1:7919449/1‑149 (MQ=255)
                                                                                                     cacaATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTAc                                            >  1:3396106/1‑149 (MQ=255)
                                                                                                        aaTTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTATACACCCCAAAAGAAATTAATACAAGGCGAGGTCTTCTTTACGGc                                         >  1:4333245/1‑149 (MQ=255)
                                                                                                         aTTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCt                                        <  1:2252872/149‑1 (MQ=255)
                                                                                                         aTTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCt                                        >  1:1712700/1‑149 (MQ=255)
                                                                                                          tttAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCtt                                       <  1:8650083/149‑1 (MQ=255)
                                                                                                          tttAACAAAGTTTATGGAAAAATGTCTCAATTTTCACTGATAAAAATATGAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGGGGTCTTCTTTACGGCtt                                       >  2:5002932/1‑149 (MQ=255)
                                                                                                           ttAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTa                                      >  2:263432/1‑149 (MQ=255)
                                                                                                           ttAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTa                                      >  1:3930970/1‑149 (MQ=255)
                                                                                                              aCAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAAcc                                   >  2:3493069/1‑149 (MQ=255)
                                                                                                               cAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGGCTTCTTTACGGCTTAACCg                                  >  1:2043391/1‑149 (MQ=255)
                                                                                                                 aaGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGAt                                <  2:7242943/149‑1 (MQ=255)
                                                                                                                 aaGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGAt                                >  1:1369352/1‑149 (MQ=255)
                                                                                                                  aGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGAtt                               <  1:5400240/149‑1 (MQ=255)
                                                                                                                    tttATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTa                             >  1:4828435/1‑149 (MQ=255)
                                                                                                                      tATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAAt                           >  2:8771167/1‑149 (MQ=255)
                                                                                                                        tGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAACAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATgg                         <  1:5802305/149‑1 (MQ=255)
                                                                                                                          gAAAAATTTGTCAATATTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAACAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGCCTTCTTTACGGCTTAACCGATTTAATgggg                       >  2:9194130/1‑148 (MQ=255)
                                                                                                                            aaaaTTTGTCAATTTTCACAGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCgg                     <  2:1614725/149‑1 (MQ=255)
                                                                                                                               aTTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAgg                  >  2:769136/1‑149 (MQ=255)
                                                                                                                                  tGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTg               >  1:4935194/1‑149 (MQ=255)
                                                                                                                                       aTTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACa          >  1:7512229/1‑149 (MQ=255)
                                                                                                                                          ttCACTGATAAAAATATAAAAATAGGAGAAGCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACAata       <  2:7425249/149‑1 (MQ=255)
                                                                                                                                           tCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACAatat      >  2:7922223/1‑149 (MQ=255)
                                                                                                                                           tCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACAatat      >  1:929914/1‑149 (MQ=255)
                                                                                                                                           tCACTGATAAAAAAATAAAAATCGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACAatat      >  2:612818/1‑149 (MQ=255)
                                                                                                                                            cACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGCCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTAGAACAACAtt     >  2:2832758/1‑149 (MQ=255)
                                                                                                                                             aCTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACAATATTg    >  1:8970993/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GTTTTATGAAGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGACAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACAATATTGTT  >  AM406671/827665‑827956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑