Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 912,618 T→A L46Q (CTA→CAA)  llmg_0944 → putative secreted protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM406671912,6180TA99.9% 3091.7 / NA 793L46Q (CTA→CAA) llmg_0944putative secreted protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (442/350);  minor base G (1/0);  total (443/350)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 795 total aligned reads displayed.

AGGAAAATAAATGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCTAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAATTTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTA  >  AM406671/912472‑912763
                                                                                                                                                  |                                                                                                                                                 
 ggAAAATAAATGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAAc                                                                                                                                                <  1:655923/149‑1 (MQ=255)
   aaaaTAAATGAATACTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAg                                                                                                                                              <  2:363398/149‑1 (MQ=255)
    aaataaatGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGa                                                                                                                                             <  2:1218287/149‑1 (MQ=255)
     aataaatGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGat                                                                                                                                            >  1:2729373/1‑149 (MQ=255)
     aataaatGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGat                                                                                                                                            >  1:7983414/1‑149 (MQ=255)
        aaatGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGataat                                                                                                                                         <  1:4391680/149‑1 (MQ=255)
         aatGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGataata                                                                                                                                        <  2:8688076/149‑1 (MQ=255)
           tGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATAtt                                                                                                                                      >  2:106898/1‑149 (MQ=255)
             aaTTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTgc                                                                                                                                    <  2:6343057/149‑1 (MQ=255)
                  tAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCaa                                                                                                                               <  1:2369181/149‑1 (MQ=255)
                   agaaaaagaaaaACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCaaa                                                                                                                              >  1:191391/1‑149 (MQ=255)
                    aaaaaagaaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCaaaa                                                                                                                             <  2:4528777/148‑1 (MQ=255)
                      aaaagaaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAAtt                                                                                                                           >  2:3888152/1‑149 (MQ=255)
                       aaagaaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAAttt                                                                                                                          <  1:7334368/149‑1 (MQ=255)
                        aagaaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTta                                                                                                                         >  2:1500988/1‑149 (MQ=255)
                        aagaaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTta                                                                                                                         <  1:6885781/149‑1 (MQ=255)
                         agaaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTtat                                                                                                                        <  1:1500988/149‑1 (MQ=255)
                          gaaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATGtata                                                                                                                       >  1:6789444/1‑149 (MQ=255)
                           aaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATAc                                                                                                                      >  2:9177081/1‑149 (MQ=255)
                            aaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATAcc                                                                                                                     <  2:7351898/149‑1 (MQ=255)
                              aaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAg                                                                                                                   <  1:1024176/149‑1 (MQ=255)
                               aCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTTGTATTGTATTGATAGGACGACACGTTATTTCTAGTCAAACAGATTATATTGCAGCAAAATTTATACCAGa                                                                                                                  >  2:8609965/1‑149 (MQ=255)
                                 agagAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAAt                                                                                                                >  2:7884065/1‑149 (MQ=255)
                                  gagaAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTtat                                                                                                                        >  2:4248006/1‑140 (MQ=255)
                                  gagaAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAAtc                                                                                                               >  2:4802825/1‑149 (MQ=255)
                                  gagaAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAAtc                                                                                                               >  1:2127059/1‑149 (MQ=255)
                                  gagaAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAAtc                                                                                                               >  1:8412911/1‑149 (MQ=255)
                                   agaagaAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAAtct                                                                                                              <  2:765544/149‑1 (MQ=255)
                                       gaaACGCAGAGGGGGGAGAGTATGTACAATATTACTCTACCTCACGATATGTCCGGCACTGCGTGTTTCGGGTATTGTAGTGCTAGGACGACACGTTATTGCTAGTCAAAGAGATAATATTGCAGCAAAATTTATAGAAGAATCTCaaa                                                                                                          >  2:5017504/1‑149 (MQ=255)
                                         aaCGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAAc                                                                                                        <  2:8124937/149‑1 (MQ=255)
                                           cGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACtt                                                                                                      >  2:7720283/1‑149 (MQ=255)
                                           cGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACtt                                                                                                      >  1:4519617/1‑149 (MQ=255)
                                               gagGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGAATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACg                                                                                                  >  2:6133419/1‑149 (MQ=255)
                                                agGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACgg                                                                                                 >  1:5717192/1‑149 (MQ=255)
                                                agGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACgg                                                                                                 >  2:1986118/1‑149 (MQ=255)
                                                   gggTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATg                                                                                              <  1:7010568/149‑1 (MQ=255)
                                                   gggTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATg                                                                                              >  1:6349865/1‑149 (MQ=255)
                                                         aGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCgg                                                                                        >  2:8372929/1‑149 (MQ=255)
                                                          gTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGa                                                                                       >  1:395245/1‑149 (MQ=255)
                                                          gTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGa                                                                                       >  2:1367585/1‑149 (MQ=255)
                                                             tttGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTTTTTTTTTTTTGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCa                                                                                    >  2:8837453/1‑149 (MQ=255)
                                                             tttGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCa                                                                                    >  1:7226126/1‑149 (MQ=255)
                                                               tGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCaaa                                                                                  <  1:5516086/149‑1 (MQ=255)
                                                                gCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAAt                                                                                 <  1:3824300/149‑1 (MQ=255)
                                                                 cAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAAtt                                                                                >  2:1660855/1‑149 (MQ=255)
                                                                 cAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAAtt                                                                                >  1:4047838/1‑149 (MQ=255)
                                                                 cAATATTACTCTCCCTTAGTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTCCAGCAAAATTTATACCAGAATCTCTAAACTTAACGGATGCAGCGGATCAAAtt                                                                                <  2:5356207/149‑1 (MQ=255)
                                                                  aaTATTACTCTCCCTTATTATATGTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGTATCAAATTg                                                                               <  2:3165656/149‑1 (MQ=255)
                                                                   atatTACTCTCCCTTATTATATTTCTGGCTCTGATTGTATTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTgg                                                                              <  1:5628652/149‑1 (MQ=255)
                                                                    tatTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGt                                                                             <  1:878165/149‑1 (MQ=255)
                                                                     atTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGtt                                                                            <  2:8503906/149‑1 (MQ=255)
                                                                      ttACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTc                                                                           >  1:7749012/1‑149 (MQ=255)
                                                                      ttACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTc                                                                           <  2:216964/149‑1 (MQ=255)
                                                                      ttACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAAACTCAAAACTTAACGGATGCAGCGGATCAAATTGGGTc                                                                           >  2:5141008/1‑149 (MQ=255)
                                                                      ttACTCTCCCTTATTACATTTCTGGCTCTGATTGTTTTTGATATTGTTATGATAGGATGACATGTTATTTCTAGTAAATCAGATAATATTGTAGCAAAATTTCTATCAGAATCTAAAAACTTACATGATGCAGCGGAGCAAGTTGAttg                                                                           >  2:6809499/1‑148 (MQ=255)
                                                                       tACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCg                                                                          <  2:6528981/149‑1 (MQ=255)
                                                                         ctctCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGc                                                                        >  1:1327971/1‑149 (MQ=255)
                                                                           ctcCCGTATTCTATTTCTGGCGCTGCTTGTGTTTGGTATTGTAATGATAAGACGACACGTCATCTCTAGTCAAACAGATAATATTGCCGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCAGCtc                                                                      >  2:9065631/1‑148 (MQ=255)
                                                                             cccTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACt                                                                    >  1:6124492/1‑149 (MQ=255)
                                                                               cTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTcc                                                                  >  2:6009701/1‑149 (MQ=255)
                                                                                ttattaTATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCg                                                                 >  1:8518943/1‑149 (MQ=255)
                                                                                 tattatATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGt                                                                >  1:1676769/1‑149 (MQ=255)
                                                                                 tattatATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGt                                                                <  2:2532783/149‑1 (MQ=255)
                                                                                  attatATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTc                                                               >  1:7507395/1‑149 (MQ=255)
                                                                                  attatATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTc                                                               <  2:7551469/149‑1 (MQ=255)
                                                                                      tatTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCt                                                           <  1:8620754/149‑1 (MQ=255)
                                                                                        tttCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTAt                                                         >  1:2976543/1‑149 (MQ=255)
                                                                                           cTGGCTCTGATTGTTTTTTGTTTTTTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTgc                                                      >  2:9020161/1‑149 (MQ=255)
                                                                                           cTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTgc                                                      <  1:4855809/149‑1 (MQ=255)
                                                                                            tGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTgca                                                     <  1:182084/149‑1 (MQ=255)
                                                                                                    aTTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGNCTACTCCGTCAGCTATTGCAAGCAAatt                                             <  1:5087713/149‑1 (MQ=255)
                                                                                                         ttttGGTATTGTAATGATAGGACGACACGTTATTTGTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATg                                        <  2:572302/149‑1 (MQ=255)
                                                                                                         ttttGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATg                                        >  1:4699872/1‑149 (MQ=255)
                                                                                                          tttGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATga                                       >  1:2277115/1‑149 (MQ=255)
                                                                                                           ttGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATgag                                      <  1:327668/149‑1 (MQ=255)
                                                                                                            tgttATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATgaga                                     >  2:4712504/4‑149 (MQ=255)
                                                                                                              gTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAAt                                   >  2:5516932/1‑149 (MQ=255)
                                                                                                                aTTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTc                                 <  2:4021035/149‑1 (MQ=255)
                                                                                                                   gTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCTGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGa                              >  1:537099/1‑149 (MQ=255)
                                                                                                                   gTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGa                              <  2:4413035/149‑1 (MQ=255)
                                                                                                                    tgaTGATAGGACGACACGTTATTTCTTGTCAAACAGATAATATTGCAGCAAAATTGATACCAGAATCTCAAGACTTAACGGATGCAGCGGATCAAATTGGTTCGGTTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGAt                             <  1:5877587/147‑1 (MQ=255)
                                                                                                                    tAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGAt                             >  1:8192433/1‑149 (MQ=255)
                                                                                                                      aTGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATAc                           >  1:254320/1‑149 (MQ=255)
                                                                                                                      aTGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATAc                           <  2:2275519/149‑1 (MQ=255)
                                                                                                                       tGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATAcc                          >  2:6977210/1‑149 (MQ=255)
                                                                                                                       tGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATAcc                          <  2:7055066/149‑1 (MQ=255)
                                                                                                                         aTAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCAt                        <  2:2974846/149‑1 (MQ=255)
                                                                                                                         aTAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCAt                        >  1:9141887/1‑149 (MQ=255)
                                                                                                                         aTAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGATACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCAt                        >  2:824252/1‑149 (MQ=255)
                                                                                                                           aGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCa                      <  2:3456866/149‑1 (MQ=255)
                                                                                                                                 acacGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTc                >  2:6714223/1‑149 (MQ=255)
                                                                                                                                  cacGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCa               >  2:8015569/1‑149 (MQ=255)
                                                                                                                                     gTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAAtc            >  2:7262291/1‑149 (MQ=255)
                                                                                                                                      ttATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAAtct           <  2:1873586/149‑1 (MQ=255)
                                                                                                                                       tATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAAtctc          >  1:8974804/1‑149 (MQ=255)
                                                                                                                                        aTTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCa         <  1:3698007/149‑1 (MQ=255)
                                                                                                                                          ttCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAgg       >  2:4082618/1‑149 (MQ=255)
                                                                                                                                             tAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGAt    <  2:2750370/149‑1 (MQ=255)
                                                                                                                                             tAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGAt    >  1:3369144/1‑149 (MQ=255)
                                                                                                                                               gTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTa  <  2:7164769/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AGGAAAATAAATGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCTAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAATTTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTA  >  AM406671/912472‑912763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑