Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 295,026 T→C S40P (TCG→CCG)  rimI2 → Ribosomal‑protein‑alanine acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM406671295,0260TC99.8% 4865.2 / NA 1189S40P (TCG→CCG) rimI2Ribosomal‑protein‑alanine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (677/509);  minor base G (2/0);  total (680/509)
Fisher's exact test for biased strand distribution p-value = 5.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 1194 total aligned reads displayed.

GCAATTTGGATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGTCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCAATATCGCCATTCA  >  AM406671/294880‑295171
                                                                                                                                                  |                                                                                                                                                 
 cAATTTGGATGGTCAAAGAATTAAAAAAATGAAATTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGc                                                                                                                                                >  2:5325544/1‑149 (MQ=255)
   aTTTGGATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCt                                                                                                                                              <  1:3203477/149‑1 (MQ=255)
   aTTTGGATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCt                                                                                                                                              <  2:7513056/149‑1 (MQ=255)
   aTTTGGATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCt                                                                                                                                              <  2:4396834/149‑1 (MQ=255)
    tttGGATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCtt                                                                                                                                             <  1:2349051/149‑1 (MQ=255)
    tttGGATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCtt                                                                                                                                             <  2:4066596/149‑1 (MQ=255)
      tGGATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTgg                                                                                                                                           >  1:686564/1‑149 (MQ=255)
       ggATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGt                                                                                                                                          >  2:7824741/1‑149 (MQ=255)
        gATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTc                                                                                                                                         >  1:3743995/1‑149 (MQ=255)
        gATGGTCAAAGAATAAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTc                                                                                                                                         <  2:2675008/149‑1 (MQ=255)
          tGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTcac                                                                                                                                       >  1:2483704/1‑149 (MQ=255)
          tGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTcac                                                                                                                                       >  2:8239913/1‑149 (MQ=255)
             tCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGACGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATAAACTTTTATGAGTGGCTTATGGACAGCCGCCTTGGTCACaaa                                                                                                                                    >  2:3035141/1‑148 (MQ=255)
             gcAAATAATTAAAAAAATGCATATAAAACGTCTGTCTTACTCTGAGAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAACATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAg                                                                                                                                    <  1:3362200/148‑1 (MQ=255)
               aaaGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGtt                                                                                                                                  >  2:2574009/1‑149 (MQ=255)
                    aTTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTtatat                                                                                                                             <  2:4910279/149‑1 (MQ=255)
                        aaaaaaTGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCg                                                                                                                         >  1:1327572/1‑149 (MQ=255)
                         aaaaaTGAATTTAAAACGGCTGTCTTAGTCTGAAAATAAGTCGAAAGAGAAACATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATAGTTATGAACTTTTATGAGTGGCTTATGGACAGCGGACTTGGTCACAAGTTTATATATcta                                                                                                                        >  2:7073227/1‑147 (MQ=255)
                          aaaaTGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAg                                                                                                                       >  1:1474601/1‑149 (MQ=255)
                          aaaaTGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAg                                                                                                                       <  2:383847/149‑1 (MQ=255)
                               gAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGAtt                                                                                                                  <  1:3059378/149‑1 (MQ=255)
                                aaTTTAAAACGTCTGCCTTACTCTGAAAATAAAAAAAAAAAAAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGAttt                                                                                                                 <  2:8855223/149‑1 (MQ=255)
                                   ttAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGt                                                                                                              >  2:6397854/1‑149 (MQ=255)
                                   ttAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGt                                                                                                              >  1:8852771/1‑149 (MQ=255)
                                   ttAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGt                                                                                                              <  2:4623667/149‑1 (MQ=255)
                                   ttAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGt                                                                                                              <  2:6125710/149‑1 (MQ=255)
                                     aaaaCGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTaa                                                                                                            <  1:262140/149‑1 (MQ=255)
                                         cGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGa                                                                                                        >  1:1020373/1‑149 (MQ=255)
                                            ctgtctTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCATCTGTGGAGAAGTTATCAGACAATATTTATGAACTTTTATCAACGGCTTATGGACAGCCGACTAGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGaaaa                                                                                                     >  2:6634940/1‑149 (MQ=255)
                                              gtcgtgctctGAAAATAGCTCGCAAGACAAAAAGACTTTTCCACCGGTGGAAAAGGTATCAGTCAATCTTTATGAACTTTTATCAGTGGCTTATTGACAGCCGCCTTGGTCACAACTTTATATATCGAGTGATTGAGTAAACGAAAAtt                                                                                                   <  2:836687/143‑1 (MQ=255)
                                               tctTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTc                                                                                                  <  1:5344931/149‑1 (MQ=255)
                                               tctTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTc                                                                                                  >  1:1814048/1‑149 (MQ=255)
                                               tctTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTc                                                                                                  >  1:4442550/1‑149 (MQ=255)
                                                ctTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCg                                                                                                 <  2:2923097/149‑1 (MQ=255)
                                                      ctGAAAATAAGTCGAAAGACAAAAATACTATTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATAc                                                                                           >  2:5663448/1‑149 (MQ=255)
                                                       tGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACt                                                                                          >  1:4576302/1‑149 (MQ=255)
                                                        gAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATCTATCGAGTGATTTAGTAAACGAAAAATCGGAATACtt                                                                                         >  2:6322952/1‑149 (MQ=255)
                                                          aaaTAAGTCGAAAGACAAAAATACTTTTCCACCTGCGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGCACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACtttt                                                                                       <  2:5798250/149‑1 (MQ=255)
                                                           aaTAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACttttt                                                                                      >  2:3340908/1‑149 (MQ=255)
                                                            aTAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTa                                                                                     <  1:4183519/149‑1 (MQ=255)
                                                             tAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTAc                                                                                    <  1:6755644/149‑1 (MQ=255)
                                                                gTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTag                                                                                 >  1:8225946/1‑149 (MQ=255)
                                                                 tCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTaga                                                                                <  2:1887768/149‑1 (MQ=255)
                                                                 tCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTaga                                                                                >  2:8610049/1‑149 (MQ=255)
                                                                 tCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTaga                                                                                >  2:7002746/1‑149 (MQ=255)
                                                                 tCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGGGATTTAGTAAACGAAAATTCGGAATACTTTTTACTaga                                                                                >  2:1490128/1‑149 (MQ=255)
                                                                  cGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTagaa                                                                               >  1:5972888/1‑149 (MQ=255)
                                                                          aaaaaTACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGa                                                                       >  1:1648425/1‑149 (MQ=255)
                                                                           aaaaTACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAg                                                                      >  1:4855716/1‑149 (MQ=255)
                                                                               tACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCaat                                                                  >  2:2447435/1‑149 (MQ=255)
                                                                                   tttCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCaattaat                                                              >  2:4790198/1‑149 (MQ=255)
                                                                                    ttCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCaattaatt                                                             <  1:7741272/149‑1 (MQ=255)
                                                                                    ttCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCaattaatt                                                             <  2:1091730/149‑1 (MQ=255)
                                                                                     tCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTg                                                            <  2:2754956/149‑1 (MQ=255)
                                                                                     tCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTg                                                            <  1:3869629/149‑1 (MQ=255)
                                                                                      ccACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTgg                                                           <  1:7368596/149‑1 (MQ=255)
                                                                                       cACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGt                                                          >  1:9205738/1‑149 (MQ=255)
                                                                                       cACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGt                                                          <  2:7690163/149‑1 (MQ=255)
                                                                                        aCCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTc                                                         >  1:2189855/1‑149 (MQ=255)
                                                                                         ccTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTct                                                        >  2:2216993/1‑149 (MQ=255)
                                                                                          cTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTctg                                                       >  1:7498072/1‑149 (MQ=255)
                                                                                           tgtgGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTctgc                                                      >  1:1186727/1‑149 (MQ=255)
                                                                                              ggAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTgg                                                   >  1:2017986/1‑149 (MQ=255)
                                                                                               gAAAAGTTATCAGTCAATATTTATGATCTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGACAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGc                                                  <  2:3720000/149‑1 (MQ=255)
                                                                                               gAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTATACGAAAATTCGGAACACTGTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGc                                                  >  1:6642969/1‑149 (MQ=255)
                                                                                                aaaaGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGcc                                                 >  2:3163144/1‑149 (MQ=255)
                                                                                                aaaaGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGcc                                                 >  2:6831495/1‑149 (MQ=255)
                                                                                                   aGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCAtt                                              >  1:8558747/1‑149 (MQ=255)
                                                                                                   aGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCAtt                                              >  1:7165508/1‑149 (MQ=255)
                                                                                                    gTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCAttt                                             <  2:1261415/149‑1 (MQ=255)
                                                                                                     ttATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTc                                            >  1:6985718/1‑149 (MQ=255)
                                                                                                      tATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCa                                           >  1:6099955/1‑149 (MQ=255)
                                                                                                      tATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCa                                           >  1:3525261/1‑149 (MQ=255)
                                                                                                       aTCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCaa                                          >  1:3641341/1‑149 (MQ=255)
                                                                                                         cAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCaata                                        <  2:5964955/149‑1 (MQ=255)
                                                                                                         cAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCaata                                        <  1:6432191/149‑1 (MQ=255)
                                                                                                              aaTATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAatgat                                   >  2:4203375/1‑149 (MQ=255)
                                                                                                                tatTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATgatgg                                 <  1:7574740/149‑1 (MQ=255)
                                                                                                                 atTTATGAACTTTTATCAGTTGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATgatgga                                <  1:78914/149‑1 (MQ=255)
                                                                                                                 atTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATgatgga                                <  2:6516113/149‑1 (MQ=255)
                                                                                                                 atTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATgatgga                                >  1:621263/1‑149 (MQ=255)
                                                                                                                 atTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATgatgga                                <  1:6324949/149‑1 (MQ=255)
                                                                                                                  tttATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATgatggat                               <  2:1612742/149‑1 (MQ=255)
                                                                                                                  tttATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTATGCTGGCCATTTCAATAATgatggat                               >  1:5149762/1‑149 (MQ=255)
                                                                                                                    tATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGa                             <  2:5469005/149‑1 (MQ=255)
                                                                                                                       gAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAAtt                          >  2:3463071/1‑149 (MQ=255)
                                                                                                                        aaCTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTa                         >  2:9119073/1‑149 (MQ=255)
                                                                                                                         aCTTTTATCAGTGGCTTATGGACAGCCGCCTTGGGCACAAGTTTATATAGCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTATTTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAg                        >  1:2810106/1‑149 (MQ=255)
                                                                                                                          cTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGa                       <  1:9253902/149‑1 (MQ=255)
                                                                                                                              tATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTg                   >  1:5632317/1‑149 (MQ=255)
                                                                                                                              tATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTg                   <  2:2136762/149‑1 (MQ=255)
                                                                                                                                tCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGAc                 <  1:834006/149‑1 (MQ=255)
                                                                                                                                   gTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCaa              >  2:7340780/1‑149 (MQ=255)
                                                                                                                                    tGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATCTCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCAat             >  1:9048621/1‑149 (MQ=255)
                                                                                                                                    tGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTCTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCAat             <  1:5796424/149‑1 (MQ=255)
                                                                                                                                      gCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCAatat           <  1:5004282/149‑1 (MQ=255)
                                                                                                                                       cTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGCTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCAATATc          <  1:8055975/149‑1 (MQ=255)
                                                                                                                                        ttATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCAATATCg         <  1:5446763/149‑1 (MQ=255)
                                                                                                                                         tATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTCCAATAATGATGGATGAAGTAGAAGTGACCAatattgg        >  2:5128978/1‑146 (MQ=255)
                                                                                                                                              aCAGCCGTCTAGGTCACAAGTTTATATATCGAGTGATTTAGTAATCGAAGATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATCATGGATGAATTAGAAGTGACCAATATCGCCATTc   <  2:804234/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCAATTTGGATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGTCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCAATATCGCCATTCA  >  AM406671/294880‑295171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑