BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,081,502	A→T	K195N (AAA→AAT)	ldh →	L‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,081,502	0	A	T	99.8%	3414.7 / NA	882	K195N (AAA→AAT)	ldh	L‑lactate dehydrogenase
Reads supporting (aligned to +/- strand): ref base A (0/1); major base T (400/479); minor base G (0/2); total (400/482)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 887 total aligned reads displayed.

GGTACTTCACTTGATACTGCACGTTTCCGTCAAGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTA  >  AM406671/1081356‑1081647

ggTACTTCACTTGATACTGCACGTTTCCGTCAAGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAttt                                                                                                                                                 <  1:7943811/149‑1 (MQ=255)

 gTACTTCACTTGATACTGCACGTTTCCGTCAAGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTg                                                                                                                                                >  2:1494605/1‑149 (MQ=255)

 gTACTTCACTTGATACTGCACGTTTCCGTCAAGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTg                                                                                                                                                >  1:567255/1‑149 (MQ=255)

  tACTTCACTTGATACTGCACGTTTCCGTCAAGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTgg                                                                                                                                               <  1:7841279/149‑1 (MQ=255)

  tACTTCACTTGATACTGCACGTTTCCGTCAAGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTgg                                                                                                                                               >  2:8032503/1‑149 (MQ=255)

      tCACTTGATACTGCACGTTTCCGTCAAGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACa                                                                                                                                           <  1:8474796/149‑1 (MQ=255)

      tCACTTGATACTGCACGTTTCCGTCAAGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACa                                                                                                                                           <  1:2141585/149‑1 (MQ=255)

      tCACTTGATACTGCACGTTTCCGTCAAGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACa                                                                                                                                           >  1:6777896/1‑149 (MQ=255)

        aCTTGATACTGCACGTTTCCGTCAAGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAAt                                                                                                                                         <  1:6223197/149‑1 (MQ=255)

         cTTGATACTGCACGTTTCCGTCAAGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATg                                                                                                                                        <  1:8117519/149‑1 (MQ=255)

         cTTGATACTGCACGTTTCCGTCAAGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATg                                                                                                                                        >  1:7064470/1‑149 (MQ=255)

                 tGCACGTTTCCGTCAAGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAg                                                                                                                                >  1:9026725/1‑149 (MQ=255)

                    aCGTTTCCGTCAAGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGaaa                                                                                                                             <  2:4107382/149‑1 (MQ=255)

                     cGTTTCCGTCAAGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATg                                                                                                                                        <  1:4370505/137‑1 (MQ=255)

                       tttCCGTCAAGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATg                                                                                                                          <  2:7606474/149‑1 (MQ=255)

                        ttCCGTCAAGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGa                                                                                                                         >  1:1011589/1‑149 (MQ=255)

                          ccGTCAAGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACt                                                                                                                       <  1:6960184/149‑1 (MQ=255)

                           cGTCAAGCATTGGCTGAAAGAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTa                                                                                                                      <  2:5510253/149‑1 (MQ=255)

                            gTCAAGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTAc                                                                                                                     <  1:8254261/149‑1 (MQ=255)

                             tCAAGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTAcc                                                                                                                    >  2:4569422/1‑149 (MQ=255)

                             tCAAGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTAcc                                                                                                                    <  1:3435910/149‑1 (MQ=255)

                             tCAAGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTAcc                                                                                                                    >  1:4830366/1‑149 (MQ=255)

                              cAAGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCt                                                                                                                   >  2:8395416/1‑149 (MQ=255)

                              cAAGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCt                                                                                                                   >  1:2654935/1‑149 (MQ=255)

                               aaGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGACTTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCtt                                                                                                                  <  2:2060243/149‑1 (MQ=255)

                               aaGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCtt                                                                                                                  <  2:4718847/149‑1 (MQ=255)

                               aaGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCtt                                                                                                                  >  1:5506844/1‑149 (MQ=255)

                                aGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTa                                                                                                                 >  1:5937354/1‑149 (MQ=255)

                                 gCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTaa                                                                                                                <  2:6275284/149‑1 (MQ=255)

                                   aTTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACg                                                                                                              >  1:1779718/1‑149 (MQ=255)

                                    ttGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGa                                                                                                             >  1:5242640/1‑149 (MQ=255)

                                       gCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGc                                                                                                          <  2:2767936/149‑1 (MQ=255)

                                              aaGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATc                                                                                                   <  1:7733143/149‑1 (MQ=255)

                                               aGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTcc                                                                                                                                   >  2:5203970/1‑116 (MQ=255)

                                               aGTTGACGTTGATGCTCGTGCAATCCACTCATACATCAGGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCg                                                                                                  <  1:7443275/149‑1 (MQ=255)

                                                 ttGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGtt                                                                                                >  1:2410638/1‑149 (MQ=255)

                                                   gACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGa                                                                                              <  2:3550126/149‑1 (MQ=255)

                                                    aCGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGaa                                                                                             <  1:1487148/149‑1 (MQ=255)

                                                    aCGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGaa                                                                                             >  1:3907527/1‑149 (MQ=255)

                                                       ttGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTg                                                                                          <  2:4364748/149‑1 (MQ=255)

                                                         gATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGtt                                                                                        >  1:1630696/1‑149 (MQ=255)

                                                          aTGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGttt                                                                                       <  2:6919331/149‑1 (MQ=255)

                                                          aTGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGttt                                                                                       <  2:6550445/149‑1 (MQ=255)

                                                          aTGCTCGTTCAATCCACGCATACATCATCGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGttt                                                                                       <  1:6326604/149‑1 (MQ=255)

                                                           tGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTg                                                                                      <  2:159273/149‑1 (MQ=255)

                                                              tCGTTCGATCGGCGCATGCATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAGCGAAGCAGAAATCGTTGAATTGTTTGAGt                                                                                   <  2:7750673/149‑1 (MQ=255)

                                                               cGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTc                                                                                  >  2:1848103/1‑149 (MQ=255)

                                                               cGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTc                                                                                  <  1:8617710/149‑1 (MQ=255)

                                                                gTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCt                                                                                 <  1:582119/149‑1 (MQ=255)

                                                                  tCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGt                                                                               <  2:6738420/149‑1 (MQ=255)

                                                                   cAATCCACGCATACATCATAGGTGAACACGGTGACTCAGAGTTTGCTGTTTGGTCACACGCTAACGTTGCAGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACATTAACGCAGAAGAAATAGTTGAAATGTTTGAGTCTGtt                                                                              >  2:2190593/1‑148 (MQ=255)

                                                                     aTCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACg                                                                            <  1:7192183/149‑1 (MQ=255)

                                                                          cGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATg                                                                       <  1:1165371/149‑1 (MQ=255)

                                                                             aTACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAg                                                                    >  1:3551885/1‑149 (MQ=255)

                                                                                   catGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACt                                                              >  1:3221305/1‑149 (MQ=255)

                                                                                   catGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACATTAACGAAGCAGAAATCGTTGAATGGTTTGAGTCTGTACGTGATGCAGCTTACt                                                              >  2:3174650/1‑149 (MQ=255)

                                                                                          gAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAatcat                                                       <  2:1334390/149‑1 (MQ=255)

                                                                                            acacGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCg                                                     <  2:7502791/149‑1 (MQ=255)

                                                                                             cacGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGc                                                    <  2:3275512/149‑1 (MQ=255)

                                                                                               cGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGCACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTa                                                  >  2:2976302/1‑149 (MQ=255)

                                                                                               cGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTa                                                  >  1:8769474/1‑149 (MQ=255)

                                                                                                 gTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTaaa                                                >  2:2559555/1‑149 (MQ=255)

                                                                                                   gACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTaaaaa                                              <  1:2310857/149‑1 (MQ=255)

                                                                                                    aCTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTaaaaaa                                             <  2:1151980/149‑1 (MQ=255)

                                                                                                    aCTCAGAATTTGCTGTTTCGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTGCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTaaaaaa                                             <  2:8695794/149‑1 (MQ=255)

                                                                                                     cTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGATCAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAg                                            <  2:563548/149‑1 (MQ=255)

                                                                                                      tCAGAATTTGCTGCTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAgg                                           <  1:65700/149‑1 (MQ=255)

                                                                                                       cAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTg                                                                                      <  2:3698739/105‑1 (MQ=255)

                                                                                                       cAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTg                                                                                      >  1:3698739/1‑105 (MQ=255)

                                                                                                        aGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTg                                         >  1:4530791/1‑149 (MQ=255)

                                                                                                        aGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTGAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTg                                         <  2:3413295/149‑1 (MQ=255)

                                                                                                         gAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGc                                        >  2:5862951/1‑149 (MQ=255)

                                                                                                          aaTTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACACTGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGAAGCTTACTCAATCATCGCTAAAAAAGGTGCa                                       >  2:4020774/1‑149 (MQ=255)

                                                                                                          aaTTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCa                                       <  2:8523177/149‑1 (MQ=255)

                                                                                                            tttGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTCTTTGAGTCTGTACGTGATGCAGCTAACTCAATCATCGCTAAAAAAGGTGCAAc                                     <  2:5357378/149‑1 (MQ=255)

                                                                                                             ttGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACa                                    <  1:3323880/149‑1 (MQ=255)

                                                                                                             ttGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACa                                    >  1:8873034/1‑149 (MQ=255)

                                                                                                               gCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACAtt                                  >  2:5985766/1‑149 (MQ=255)

                                                                                                               gCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACAtt                                  <  1:9150562/149‑1 (MQ=255)

                                                                                                                 tGTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCt                                <  2:2323112/149‑1 (MQ=255)

                                                                                                                  gTTTGGTCACACGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTa                               <  2:7306860/149‑1 (MQ=255)

                                                                                                                       gTCACACGCTACCGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGgtg                          >  1:4681545/1‑149 (MQ=255)

                                                                                                                         cacaCGCTAGCGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAACATGACTACCTTAACGAAGCAGAAATCGTTTAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGtgtg                        <  2:2894980/149‑1 (MQ=255)

                                                                                                                          acacGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTgg                       <  2:3912338/149‑1 (MQ=255)

                                                                                                                          acacGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTgg                       <  1:2874736/149‑1 (MQ=255)

                                                                                                                          acacGCTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTgg                       >  1:6665/1‑149 (MQ=255)

                                                                                                                               ttAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTAGGTGATGCAGCTTACTCAATCATCGCTAAAAAGGGTGCAACATTCTACGGTGTGGCTGTa                  <  2:7856371/148‑1 (MQ=255)

                                                                                                                               cTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTa                  <  2:9056706/149‑1 (MQ=255)

                                                                                                                               cTAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTa                  <  2:8143109/149‑1 (MQ=255)

                                                                                                                                tAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAg                 <  2:9220064/149‑1 (MQ=255)

                                                                                                                                tAACGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAg                 >  2:4987284/1‑149 (MQ=255)

                                                                                                                                  aCGTTGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGcc               >  1:5012024/1‑149 (MQ=255)

                                                                                                                                   cGTTGCTGGTGTGAATTTGGGACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGccc              <  1:2013933/149‑1 (MQ=255)

                                                                                                                                     ttGCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCtt            <  1:969500/149‑1 (MQ=255)

                                                                                                                                       gCTGGTGTTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGc          >  1:4158888/1‑149 (MQ=255)

                                                                                                                                       gCTGGTGTGAATTTGGAACAATGGTTCCAAGAAAATGACTACATTAGCGAAGCAGAAATCGTTGAATTGTTTGAGGATGTACGTGATGCAGCTTACTCAGTCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAgaatttgc          <  2:2842692/149‑8 (MQ=255)

                                                                                                                                            tgtTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTa     >  2:8864550/1‑149 (MQ=255)

                                                                                                                                             gtTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCACTCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTAt    >  2:71248/1‑149 (MQ=255)

                                                                                                                                             gtTAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTAt    >  1:1362659/1‑149 (MQ=255)

                                                                                                                                              ttAATTTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTAtt   >  1:1896024/1‑149 (MQ=255)

GGTACTTCACTTGATACTGCACGTTTCCGTCAAGCATTGGCTGAAAAAGTTGACGTTGATGCTCGTTCAATCCACGCATACATCATGGGTGAACACGGTGACTCAGAATTTGCTGTTTGGTCACACGCTAACGTTGCTGGTGTTAAATTGGAACAATGGTTCCAAGAAAATGACTACCTTAACGAAGCAGAAATCGTTGAATTGTTTGAGTCTGTACGTGATGCAGCTTACTCAATCATCGCTAAAAAAGGTGCAACATTCTACGGTGTGGCTGTAGCCCTTGCTCGTATTA  >  AM406671/1081356‑1081647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`