Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 1,277,684 T→A N231I (AAT→ATT)  llmg_1306 ← conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM4066711,277,6840TA99.9% 3541.2 / NA 904N231I (AAT→ATT) llmg_1306conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (433/470);  minor base C (1/0);  total (434/470)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 904 total aligned reads displayed.

CTGAATTTTGTAAAATTTTACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCATTAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCTTTCAGGCC  >  AM406671/1277538‑1277829
                                                                                                                                                  |                                                                                                                                                 
 tGAATTTTGTAAAATTTTACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATaa                                                                                                                                                >  2:8059202/1‑149 (MQ=255)
      tttGTAAAATTTTACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGaa                                                                                                                                           >  2:3731617/1‑149 (MQ=255)
        tGTAAAATTTTACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAACAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTAGTTGTCTCAATAAAAGAAcc                                                                                                                                         >  2:6035567/1‑149 (MQ=255)
               ttttACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAggg                                                                                                                                  <  1:8446724/149‑1 (MQ=255)
                tttACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGt                                                                                                                                 <  1:3225983/149‑1 (MQ=255)
                 ttAATCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGtt                                                                                                                                <  2:763887/149‑1 (MQ=255)
                   aCTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTc                                                                                                                              >  2:6691176/1‑149 (MQ=255)
                     tCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCt                                                                                                                            <  1:1614951/149‑1 (MQ=255)
                     tCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCt                                                                                                                            <  1:1509514/149‑1 (MQ=255)
                     tCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCt                                                                                                                            >  1:2717478/1‑149 (MQ=255)
                     tCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCt                                                                                                                            <  2:3427402/149‑1 (MQ=255)
                      cGTATCTAACCATGCAATTTGGCTCCAATGTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTa                                                                                                                           >  2:5654854/1‑149 (MQ=255)
                       gTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAg                                                                                                                          >  1:2011447/1‑149 (MQ=255)
                        tATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGa                                                                                                                         <  1:6710199/149‑1 (MQ=255)
                        tATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGa                                                                                                                         >  1:719731/1‑149 (MQ=255)
                         aTCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGAt                                                                                                                        <  1:5831524/149‑1 (MQ=255)
                         aTCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGAt                                                                                                                        <  1:7369724/149‑1 (MQ=255)
                         aTCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGAt                                                                                                                        >  2:7098013/1‑149 (MQ=255)
                         aTCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGAt                                                                                                                        >  2:7883622/1‑149 (MQ=255)
                         aTCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTAAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTAGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGAt                                                                                                                        >  2:8770578/1‑149 (MQ=255)
                           cTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGCTTCCTAGAtgg                                                                                                                      >  2:6917451/1‑148 (MQ=255)
                             aaCCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGc                                                                                                                    <  1:7033916/149‑1 (MQ=255)
                             aaCCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGc                                                                                                                    >  1:7760380/1‑149 (MQ=255)
                              aCCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGCAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACCATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAACGGGTTTCCTAGATGTGcc                                                                                                                   >  2:4553610/1‑148 (MQ=255)
                              aCCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCt                                                                                                                   >  1:3626504/1‑149 (MQ=255)
                                cATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTcc                                                                                                                 >  1:8608206/1‑149 (MQ=255)
                                cATGCAATTTCGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTcc                                                                                                                 <  2:3601618/149‑1 (MQ=255)
                                cATGCAATTTCGCTCCAACCTCCTCCAGCAAAACCTGCTTGAGTGGAAAAAGTTACGGTGCGCCCAGAATTACAACGCGGACTATTAGCTGCGGTCGGTGTCGGTGTTGTCTCAATAAAAGAACCGAAAGGGTTCCCTAGATGTGCGcc                                                                                                                 >  2:3225664/1‑149 (MQ=255)
                                 attcAATGTGGCTCCAATCTCCAAAAACAAAACAATCTTCAGTGGAAAAAGATACTGTTAATACAAAATTTAAAAGAGGAATATAAGCTGCCGACGGTGTCGTTGTTGTCTCAATAAAAGAACCGCAAGGGTTTCCTAGATGTGCGCCt                                                                                                                <  2:1099302/146‑1 (MQ=255)
                                    cAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTa                                                                                                             <  1:3128126/149‑1 (MQ=255)
                                      aTTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACGATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGCTTCCTAGATGTGCTCCTTGAtt                                                                                                           >  1:6473732/1‑149 (MQ=255)
                                        ttGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTAtttt                                                                                                         <  1:6280964/149‑1 (MQ=255)
                                         tGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAANGGTTTCCTAGATGTGCTCCTTTATTTTc                                                                                                        <  1:5095803/149‑1 (MQ=255)
                                         tGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTc                                                                                                        >  1:2319744/1‑149 (MQ=255)
                                         tGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTc                                                                                                        <  1:4352712/149‑1 (MQ=255)
                                          ggCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCa                                                                                                       >  2:5081426/1‑149 (MQ=255)
                                          ggCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCa                                                                                                       <  2:7218156/149‑1 (MQ=255)
                                           gCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAAATGTGCTCCTTTATTTTCAg                                                                                                      <  2:8985472/149‑1 (MQ=255)
                                             tCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAggg                                                                                                    >  2:4248347/1‑149 (MQ=255)
                                              ccAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGt                                                                                                   <  1:4718739/149‑1 (MQ=255)
                                              ccAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGt                                                                                                   >  1:4211536/1‑149 (MQ=255)
                                                   ctcCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTAtt                                                                                              >  1:1867990/1‑149 (MQ=255)
                                                    tcCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTAttt                                                                                             >  2:5864780/1‑149 (MQ=255)
                                                     ccTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTc                                                                                            >  2:9152416/1‑149 (MQ=255)
                                                          aGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGc                                                                                       >  1:3850/1‑149 (MQ=255)
                                                              aaaCCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTaa                                                                                   >  1:8218306/1‑149 (MQ=255)
                                                                 ccTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCt                                                                                <  1:6837713/149‑1 (MQ=255)
                                                                   tGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTAt                                                                              >  1:6002777/1‑149 (MQ=255)
                                                                      ttCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGAt                                                                           <  2:4010475/149‑1 (MQ=255)
                                                                      ttCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGAt                                                                           <  2:6812705/149‑1 (MQ=255)
                                                                           tGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTatca                                                                      <  2:2231524/149‑1 (MQ=255)
                                                                            ggAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTatcat                                                                     <  2:1841100/149‑1 (MQ=255)
                                                                               aaaaGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTatcatcat                                                                  >  1:7624563/1‑149 (MQ=255)
                                                                               aaaaGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTatcatcat                                                                  <  1:7462426/149‑1 (MQ=255)
                                                                                  aGTTACTGTGCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTCCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATcatcatcat                                                               <  2:2584616/149‑1 (MQ=255)
                                                                                    ttACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCAGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTa                                                             <  2:6350268/149‑1 (MQ=255)
                                                                                      aCTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACt                                                           <  2:4429016/149‑1 (MQ=255)
                                                                                               ccAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTgagg                                                  <  2:4662748/149‑1 (MQ=255)
                                                                                                cAGAATTTAAAAGCGGACTATTAGCTCCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTgagga                                                 <  2:1990048/149‑1 (MQ=255)
                                                                                                 aGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTgaggag                                                <  1:8311389/149‑1 (MQ=255)
                                                                                                   aaTTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTa                                              <  2:4148347/149‑1 (MQ=255)
                                                                                                   aaTTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTa                                              <  2:6202705/149‑1 (MQ=255)
                                                                                                     tttAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTAtt                                            <  2:7547865/149‑1 (MQ=255)
                                                                                                      ttAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATACTTACTTTGTTGAGGAGTATTc                                           >  1:1776884/1‑149 (MQ=255)
                                                                                                       tAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTcc                                          >  1:5357251/1‑149 (MQ=255)
                                                                                                          aaGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAAt                                       <  2:4955340/149‑1 (MQ=255)
                                                                                                           aGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATa                                      >  1:4541346/1‑149 (MQ=255)
                                                                                                            gCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGTAAGGGTGTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAg                                     <  2:162833/149‑1 (MQ=255)
                                                                                                            gCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAg                                     >  2:3100066/1‑149 (MQ=255)
                                                                                                            gCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAg                                     <  2:1386023/149‑1 (MQ=255)
                                                                                                            gCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAg                                     <  2:8314681/149‑1 (MQ=255)
                                                                                                             cGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAgg                                    <  1:548583/149‑1 (MQ=255)
                                                                                                                aCTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTAt                                 >  1:2513433/1‑149 (MQ=255)
                                                                                                                 cTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCCTCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATc                                >  2:6439702/1‑149 (MQ=255)
                                                                                                                   aTTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCaa                              >  1:9060783/1‑149 (MQ=255)
                                                                                                                     tAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAAt                            >  2:3854205/1‑149 (MQ=255)
                                                                                                                        cTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTta                         <  2:4874010/149‑1 (MQ=255)
                                                                                                                          gCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTtata                       <  1:3991384/149‑1 (MQ=255)
                                                                                                                             gtcggtGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAAcc                    >  1:804844/1‑149 (MQ=255)
                                                                                                                             gtcggtGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAAcc                    >  2:5553006/1‑149 (MQ=255)
                                                                                                                             gtcggtGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAAcc                    <  1:339019/149‑1 (MQ=255)
                                                                                                                              tcggtGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCa                   >  1:1039325/1‑149 (MQ=255)
                                                                                                                              tcggtGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCa                   >  1:1216201/1‑149 (MQ=255)
                                                                                                                                ggtGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAAt                 <  2:2701125/149‑1 (MQ=255)
                                                                                                                                  tgtCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAg               <  2:8596952/149‑1 (MQ=255)
                                                                                                                                   gtCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGc              <  1:1345952/149‑1 (MQ=255)
                                                                                                                                    tCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCt             >  1:2119123/1‑149 (MQ=255)
                                                                                                                                     cGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTc            <  1:2916310/149‑1 (MQ=255)
                                                                                                                                     cGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTc            >  2:5426277/1‑149 (MQ=255)
                                                                                                                                     cGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGCATTCCAATAGGTATCAAATTTATAACCACTAGCTc            >  2:7989271/1‑149 (MQ=255)
                                                                                                                                        tgttgtCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCtt         >  1:918943/1‑149 (MQ=255)
                                                                                                                                        tgttgtCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCtt         >  1:5646916/1‑149 (MQ=255)
                                                                                                                                         gttgtCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCttt        <  2:7442659/149‑1 (MQ=255)
                                                                                                                                         gttgtCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCttt        <  2:2937591/149‑1 (MQ=255)
                                                                                                                                         gttgtCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCttt        <  1:8852988/149‑1 (MQ=255)
                                                                                                                                         gttgtCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCttt        <  2:5245932/149‑1 (MQ=255)
                                                                                                                                          ttgtCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCTTTc       >  2:2824788/1‑149 (MQ=255)
                                                                                                                                          ttgtCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCTTTc       >  1:7913507/1‑149 (MQ=255)
                                                                                                                                          ttgtCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCTTTc       >  1:4841355/1‑149 (MQ=255)
                                                                                                                                          ttgtCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCTTTc       <  1:4989569/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CTGAATTTTGTAAAATTTTACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCATTAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCTTTCAGGCC  >  AM406671/1277538‑1277829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑