BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,492,365	T→A	L262L (CTA→CTT)	kinE ←	sensor kinase protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,492,365	0	T	A	99.4%	3206.7 / NA	863	L262L (CTA→CTT)	kinE	sensor kinase protein
Reads supporting (aligned to +/- strand): ref base T (1/1); major base A (433/422); minor base C (3/2); total (437/426)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 863 total aligned reads displayed.

GCAGGTTGAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGATAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAATTTTTTGATTTAATC  >  AM406671/1492219‑1492510

  aGGTTGAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCg                                                                                                                                               >  1:3243994/1‑149 (MQ=255)

    gTTGAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAGGTTAAATGTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAGATTGTCAGTAAATCTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAGGCAAGCGAg                                                                                                                                             >  1:7658430/1‑149 (MQ=255)

     ttGAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGt                                                                                                                                            >  1:7292023/1‑149 (MQ=255)

      tGAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGtc                                                                                                                                           <  2:6285449/149‑1 (MQ=255)

      tGAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATCGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGtc                                                                                                                                           >  1:3048279/1‑149 (MQ=255)

       gAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGtca                                                                                                                                          >  1:7170046/1‑149 (MQ=255)

       gAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGtca                                                                                                                                          <  2:8738374/149‑1 (MQ=255)

        aaTTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGTCTCTTCATCAATATAATAAAGTGAGtcaa                                                                                                                                         >  2:223705/1‑148 (MQ=255)

         aTTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGACATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGtcatc                                                                                                                                        >  2:5282552/1‑149 (MQ=255)

             cACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTAACTTCCAAATGATTTCCCCGCGAAATTGTCATTAAATTTTCAATTAGCCCTTACATTCTAACCGACTATTGATCAATCTACGAAACCGAGTCATCaata                                                                                                                                    >  2:4122148/1‑149 (MQ=255)

               cTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCaataat                                                                                                                                  >  1:7021101/1‑149 (MQ=255)

                 gACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGATCCCTTTCATTTTCCGGGTCCCTTCATCAATATAACAAAGCGAGTCATCAATAAttt                                                                                                                                >  2:3736595/1‑149 (MQ=255)

                  aCCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTc                                                                                                                               <  1:7483539/149‑1 (MQ=255)

                     aCTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTGCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATGCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCggg                                                                                                                            <  1:5676363/149‑1 (MQ=255)

                      cTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGa                                                                                                                           <  2:5721246/149‑1 (MQ=255)

                      cTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTGCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGa                                                                                                                           <  1:132177/149‑1 (MQ=255)

                       tttGTCAGGAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGAt                                                                                                                          <  1:6722471/149‑1 (MQ=255)

                           tCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCGCGAGAAATTGGCAGTAAATTTGCAATGAGCCCTATCATTCTCAGTGACTCTACATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATgtgc                                                                                                                      >  2:6646392/1‑146 (MQ=255)

                           tCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTc                                                                                                                      <  1:6008914/149‑1 (MQ=255)

                            cAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCACGAGAAATTGTCAGTAACTTTTCAATGAGCCCTTCCATTCTCTGGGACTCTGCATCAATATAAGAAAGCGAGTCAGCAATAATTTCGTGATGCTCa                                                                                                                     >  2:1436077/1‑149 (MQ=255)

                               tAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGc                                                                                                                  >  2:202884/1‑149 (MQ=255)

                                aaaTTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGcc                                                                                                                 <  2:7093797/149‑1 (MQ=255)

                                     cAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCAtctt                                                                                                            >  2:2922019/1‑149 (MQ=255)

                                       gTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCAtcttct                                                                                                          <  1:4836982/149‑1 (MQ=255)

                                        tCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATcttctt                                                                                                         >  1:4027624/1‑149 (MQ=255)

                                              tAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAACCCTTTCATTCTCAGGGTGTCATCATCATTTTAAGAAAGCGAGTTATCAACAATTTCGGGCTGCTCGTGCCATCTTTTTCTttat                                                                                                   >  2:918387/1‑149 (MQ=255)

                                               aaaTTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTtaata                                                                                                  >  1:4441009/1‑149 (MQ=255)

                                               aaaTTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTtaata                                                                                                  >  1:4189120/1‑149 (MQ=255)

                                                aaTTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTtaataa                                                                                                 <  2:435189/149‑1 (MQ=255)

                                                 aTTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGTGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATaaa                                                                                                <  2:8578664/149‑1 (MQ=255)

                                                 aTTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGAGAGTCATCAATAATTTCGTGATGCTCATGCCATCTTCTTTTTAATaaa                                                                                                >  2:1528988/1‑149 (MQ=255)

                                                   ttttCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGt                                                                                              >  1:8215047/1‑149 (MQ=255)

                                                       cTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACa                                                                                          <  2:4027745/149‑1 (MQ=255)

                                                         ttatttaCTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGCCTCTTCATCAATATAAGAAAGCGATTCATAAATAATTTCGGGATGCACATGCCATCTGCTTTTTAATAAAGTGACATg                                                                                        >  2:7769605/1‑149 (MQ=255)

                                                         ttatttaCTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATg                                                                                        <  1:1208404/149‑1 (MQ=255)

                                                             ttaCTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCa                                                                                    >  1:6475744/1‑149 (MQ=255)

                                                             ttaCTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCa                                                                                    <  1:2124189/149‑1 (MQ=255)

                                                               aCTTCCAAATGATTGCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACg                                                                                  <  1:1606604/149‑1 (MQ=255)

                                                                cTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGa                                                                                 >  2:7668762/1‑149 (MQ=255)

                                                                 ttCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAATCGAGTCATCAAGAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGaa                                                                                <  1:231263/149‑1 (MQ=255)

                                                                 ttCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTAAATGAGCACTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGaa                                                                                <  2:3641305/149‑1 (MQ=255)

                                                                  tCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTcc                                                                                     <  2:5438770/143‑1 (MQ=255)

                                                                     aaaTGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTgc                                                                            >  1:1017110/1‑149 (MQ=255)

                                                                       aTGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTgcag                                                                          >  1:3525431/1‑149 (MQ=255)

                                                                        tGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTgcagc                                                                         >  2:7964468/1‑149 (MQ=255)

                                                                           tttCCCCGAGAAATTGTCAGTAATTTTTCCACTACGCCTATCATCCTCAGAGACTCTTCATCAATATAAGAAAGCGATCCATCAATACTTTCGGGATGCTCATTCCATCTTCTTTTTAATAAACTGACATGTCCACGAATTGCAGCAAc                                                                      <  2:327078/149‑1 (MQ=255)

                                                                           tttCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAAc                                                                      >  1:8354825/1‑149 (MQ=255)

                                                                           tttCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAAc                                                                      <  1:4628617/149‑1 (MQ=255)

                                                                                 cGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGTGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGt                                                                >  2:841876/1‑149 (MQ=255)

                                                                                  gagaAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTACAGGAATTGCAGAAACGGGCGTc                                                               >  2:5890958/1‑149 (MQ=255)

                                                                                   agaAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTcc                                                              <  1:9034393/149‑1 (MQ=255)

                                                                                   agaAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTcc                                                              >  1:1908515/1‑149 (MQ=255)

                                                                                   agaAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGGCATCAATAATTTCGGGATGCTAATGCCATCTTCTTTTTAATAAGGTGACATGTCCCTGAATTGCAGCAACGGGCGTcc                                                              >  1:1450175/1‑149 (MQ=255)

                                                                                    gaAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCt                                                             >  1:8895318/1‑149 (MQ=255)

                                                                                    gaAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCt                                                             >  2:128716/1‑149 (MQ=255)

                                                                                     aaaTTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTc                                                            <  2:5541343/149‑1 (MQ=255)

                                                                                      aaTTGTCAGTAAATTTTCAATGAGCCCTTTCATCCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTGTTAATAAAGTGACATGTCCAAGACTTGCAGCAAAGGGCGCCCTCa                                                           >  2:6928936/1‑149 (MQ=255)

                                                                                       aTTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAg                                                          >  2:7399884/1‑149 (MQ=255)

                                                                                        ttGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGt                                                         >  1:8140186/1‑149 (MQ=255)

                                                                                           tCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGTGTCATGAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCCCGAATTGCAGCAACGGGCGTCTTCAGTTCa                                                      >  1:8501368/1‑149 (MQ=255)

                                                                                            cAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCAt                                                     <  2:646744/149‑1 (MQ=255)

                                                                                             aGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCAtg                                                    <  1:2621707/149‑1 (MQ=255)

                                                                                             aGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCAtg                                                    >  2:5053427/1‑149 (MQ=255)

                                                                                                aaaTTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGa                                                 >  2:886993/1‑149 (MQ=255)

                                                                                                 aaTTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGTAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACCTGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGaa                                                <  2:179453/149‑1 (MQ=255)

                                                                                                 aaTTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGaa                                                >  1:6335998/1‑149 (MQ=255)

                                                                                                   ttttCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGc                                              >  2:1011727/1‑149 (MQ=255)

                                                                                                   ttttCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGc                                              <  1:5012003/149‑1 (MQ=255)

                                                                                                    tttCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCa                                             <  1:684642/149‑1 (MQ=255)

                                                                                                     ttaaaTGAGCCCTGGCATTCACAGCGACTCTTCATCATTATAAGAAAGCGAGTAATCAATAATTTCGGGATGCTCATACCTTCTGCTTTTTAATAACGTTATATGGCCACGAATCTCCCCATCGGGCGCCCTCAGTTATTGTGAAGCAt                                            >  2:7245175/4‑149 (MQ=255)

                                                                                                        aaTGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCg                                         <  1:5582934/149‑1 (MQ=255)

                                                                                                         attaGCCCTTTCATTCTCAAGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTAATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGa                                        <  2:3052124/146‑1 (MQ=255)

                                                                                                          tGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGaa                                       >  1:8655194/1‑149 (MQ=255)

                                                                                                           gAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGaaa                                      >  1:249935/1‑149 (MQ=255)

                                                                                                               ccTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGaaacaaa                                  <  1:8003884/149‑1 (MQ=255)

                                                                                                               ccTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGaaacaaa                                  <  2:2789801/149‑1 (MQ=255)

                                                                                                               ccTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGAGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGaaacaaa                                  <  1:2771746/149‑1 (MQ=255)

                                                                                                                 tttCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAAct                                >  2:6821410/1‑149 (MQ=255)

                                                                                                                  ttCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAActg                               <  1:5393708/149‑1 (MQ=255)

                                                                                                                  ttCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAActg                               <  1:3128790/149‑1 (MQ=255)

                                                                                                                   tCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAActgc                              <  2:4520600/149‑1 (MQ=255)

                                                                                                                   tCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAActgc                              <  1:3705185/149‑1 (MQ=255)

                                                                                                                   tCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAActgc                              >  1:1761678/1‑149 (MQ=255)

                                                                                                                      ttCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACtgctgc                           >  1:3869123/1‑149 (MQ=255)

                                                                                                                        ctcAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTc                         <  1:7881787/149‑1 (MQ=255)

                                                                                                                        ctcAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTc                         >  2:2425725/1‑149 (MQ=255)

                                                                                                                           aGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCAtt                      <  2:825219/149‑1 (MQ=255)

                                                                                                                             ggACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATtct                    >  1:6547849/1‑149 (MQ=255)

                                                                                                                             ggACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATtct                    >  2:1648409/1‑149 (MQ=255)

                                                                                                                             ggACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTACTCTGTTCATGTGATGCATCCGAAACAAACTGCTGCTCATtct                    >  1:6625923/1‑149 (MQ=255)

                                                                                                                                 tctTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAAt                <  2:7165813/149‑1 (MQ=255)

                                                                                                                                      atcaATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAAtttttt           >  1:583533/1‑149 (MQ=255)

                                                                                                                                      atcaATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAAtttttt           >  1:2865087/1‑149 (MQ=255)

                                                                                                                                       tcaATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAATTTTTTg          >  2:4860146/1‑149 (MQ=255)

                                                                                                                                          atatAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAATTTTTTGAtt       <  2:7577476/149‑1 (MQ=255)

                                                                                                                                             taAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAATTTTTTGATTTaa    >  1:5450889/1‑149 (MQ=255)

                                                                                                                                              aaGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAATTTTTTGATTTAAt   >  2:2608864/1‑149 (MQ=255)

                                                                                                                                              aaGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAATTTTTTGATTTAAt   <  1:9235032/149‑1 (MQ=255)

                                                                                                                                              aaGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCCCATTCTCAATTTTTTGATTTAAt   >  2:4717725/1‑149 (MQ=255)

                                                                                                                                               aGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAATTTTTTGATTTAATc  <  2:9031179/149‑1 (MQ=255)

GCAGGTTGAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGATAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAATTTTTTGATTTAATC  >  AM406671/1492219‑1492510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`