BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,572,258	G→A	intergenic (+56/+181)	llmg_1598 → / ← deoD	putative secreted protein/DeoD protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,572,258	0	G	A	100.0%	3335.2 / NA	843	intergenic (+56/+181)	llmg_1598/deoD	putative secreted protein/DeoD protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (401/442); total (401/442)

Only 100 of 847 total aligned reads displayed.

TCTTGGACAAAAGGTTCAACAGAAAGTGTTACCGTTCAATACGACAAAGCTTCTGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAATAGAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAACAGAAATTTACTTAGATTGAGAA  >  AM406671/1572113‑1572400

  ttGGACAAAAGGTTCAACAGAAAGTGTTACCGTTCAATACGACAAAGCTTCTGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCtt                                                                                                                                            <  1:8591514/149‑1 (MQ=255)

    ggACAAAAGGTTTTACAGAAATTGTTACCGTTCAATACGACAAAGCTTCTGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGTtttt                                                                                                                                          >  2:1319271/1‑149 (MQ=255)

      aCAAAAGGTTCAACAGAAAGTGTTACCGTTCAATACGACAAAGCTTCTGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGa                                                                                                                                        >  1:5930264/1‑149 (MQ=255)

       cAAAAGGTTCAACAGAAAGTGTTACCGTTCAATACGACAAAGCTTCTGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGaa                                                                                                                                       >  1:9158884/1‑149 (MQ=255)

         aaaGGTTCAACAGAAAGTGTTACCGTTCAATACGACAAAGCTTCTGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATa                                                                                                                                     <  1:8968952/149‑1 (MQ=255)

          aaGGTTCAACAGAAAGTGTTACCGTTCAATACGACAAAGCTTCTGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATaa                                                                                                                                    <  1:2852902/149‑1 (MQ=255)

               tCAACAGAAAGTGTTACCGTTCAATACGACAAAGCTTCTGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCttt                                                                                                                               <  1:1432819/149‑1 (MQ=255)

               tCAACAGAAAGTGTTACCGTTCAATACGACAAAGCTTCTGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCttt                                                                                                                               >  1:2535828/1‑149 (MQ=255)

                cAACAGAAAGTGTTACCGTTCAATACGACAAAGCTTCTGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCtttt                                                                                                                              <  1:3409034/149‑1 (MQ=255)

                   cAGAAAGTGTTACCGTTCAATACGACAAAGCTTCTGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTa                                                                                                                           >  2:2722383/1‑149 (MQ=255)

                     gAAGGTGTTACCGGTCAATACGAAAAAGCTTCTGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGGTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTAtt                                                                                                                         <  2:2461026/149‑1 (MQ=255)

                       aaGTGTTACCGTTCAATACGACAAAGCTTCTGGTCAAATTACAAGTAAATCTACATTTAATGCATAAGAAACTTTACATCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAGAAAAGCTATTGAATAAGCTTTTTTAtttt                                                                                                                       >  2:5389069/1‑149 (MQ=255)

                       aaGTGTTACCGTTCAATACGACAAAGCTTCTGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTAtttt                                                                                                                       >  1:7273624/1‑149 (MQ=255)

                         gtgtTACCGTTCAATACGACAAAGCTTCTGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCa                                                                                                                     >  1:6259214/1‑149 (MQ=255)

                              aaagTTAAATACGACAAAGCTTCTAGTCAAATTACAAATAAAACTAAATTTAATGAATAAGAAACTTTAACTCAAATCTAAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAAt                                                                                                                <  2:1085626/146‑1 (MQ=255)

                              aCCGTTCAATACGACAAAGCTTCTGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAAt                                                                                                                <  2:8967247/149‑1 (MQ=255)

                              aCCGTTCAATACGACAAAGCTTCTGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAAt                                                                                                                <  1:8405153/149‑1 (MQ=255)

                                  ttCAATACGACAAAGCTTCTGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTAACTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACa                                                                                                            <  2:8742687/149‑1 (MQ=255)

                                    cAATACGACAAAGCTTCTGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACaga                                                                                                          >  1:5589236/1‑149 (MQ=255)

                                      aTACGACAAAGCTTCTGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACagaag                                                                                                        <  1:570672/149‑1 (MQ=255)

                                       tACGACAAAGCTTCTGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACagaaga                                                                                                       >  1:3766129/1‑149 (MQ=255)

                                        aCGACAAAGCTTCTGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGAt                                                                                                      >  2:6405391/1‑149 (MQ=255)

                                        aCGACAAAGCTTCTGGTCAAATTAAAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATATAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGAt                                                                                                      <  2:8527487/149‑1 (MQ=255)

                                           aCAAAGCTTCTGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATTGa                                                                                                   >  2:3098807/1‑149 (MQ=255)

                                                 cTTCTGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAActc                                                                                             >  1:4207425/1‑149 (MQ=255)

                                                 cTTCTGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAActc                                                                                             >  1:3121408/1‑149 (MQ=255)

                                                  ttCTGGTAAAATTACAAGTAAAACTAAATTTAATGAATAAGAAACATTACATCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAAAAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAActct                                                                                            <  2:5616359/149‑1 (MQ=255)

                                                   tCTGATAAAATTAAAAATAAATCTAAATTTAATGAATAAGAAACTTTACCACATCTCTGAAAAGATGAAAGGAAAGTTTATATAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACtctt                                                                                           <  2:7521684/149‑1 (MQ=255)

                                                    ttagTCAAAATACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACtcttc                                                                                          <  2:40095/146‑1 (MQ=255)

                                                     tGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACtcttct                                                                                         <  1:3521139/149‑1 (MQ=255)

                                                       gTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCtaa                                                                                       >  1:4928429/1‑149 (MQ=255)

                                                       gTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCtaa                                                                                       <  1:6110251/149‑1 (MQ=255)

                                                        tCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCtaat                                                                                      >  1:8707234/1‑149 (MQ=255)

                                                         cAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCtaata                                                                                     >  2:6234072/1‑149 (MQ=255)

                                                         aaaaTTACAAGAAAATCTAAAAATAATGCATAAGAAACTCTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATATAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCtaata                                                                                     <  2:7710857/148‑1 (MQ=255)

                                                         aaaaTTAAAAGAAAATATAAATGTAATGAATAAGAAACTTTATCTCATCTCTGAAAAGATTGAAAGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCtaata                                                                                     <  2:6935723/148‑1 (MQ=255)

                                                          aaaTTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCtaataa                                                                                    >  2:3857492/1‑149 (MQ=255)

                                                          aaaTTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCtaataa                                                                                    <  2:5114428/149‑1 (MQ=255)

                                                           aaTTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGAt                                                                                                      >  2:5958545/1‑130 (MQ=255)

                                                           aaTTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATaaa                                                                                   <  2:1534431/149‑1 (MQ=255)

                                                             ttACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATCTTCAGAAATCACAGCAGATGGAAAAATCTTATACTcaaac                                                                                 >  2:882433/1‑148 (MQ=255)

                                                                        tCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGt                                                                      >  1:7090758/1‑149 (MQ=255)

                                                                        tCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGt                                                                      >  2:2371816/1‑149 (MQ=255)

                                                                         cTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTg                                                                     <  2:8250351/149‑1 (MQ=255)

                                                                         cTAAAATGAATGCATAAGAAACCTCAACCCAAGTCGCAACAGATGTAAAGAAAGTATATCCAAAATGAAAAAAAGCTTAAGAATAACCCTTTTTATCTTCAGAAAGAGCCGAAGATGGAAAACTCGTCTAATAAAATAGAACTTGAGTg                                                                     <  2:3275509/149‑1 (MQ=255)

                                                                           aaaTTTAATGAATAAGAAAATTAACATCATCTATAAAAAGATAGAAGAAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGAt                                                                   <  2:4619940/149‑1 (MQ=255)

                                                                               ttAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTGTAATAAAATAGAAATTGAGTGATaaaa                                                               >  1:397565/1‑149 (MQ=255)

                                                                                tAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATaaaaa                                                              >  1:7555936/1‑149 (MQ=255)

                                                                                 aaTGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATaaaaaa                                                             <  1:1716715/149‑1 (MQ=255)

                                                                                  aTGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAAt                                                            >  1:7015233/1‑149 (MQ=255)

                                                                                  aTGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAAt                                                            <  1:8841860/149‑1 (MQ=255)

                                                                                  aTGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGAAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAAt                                                            <  2:1620525/149‑1 (MQ=255)

                                                                                   tGCATAAGACACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATa                                                           >  1:4758228/1‑149 (MQ=255)

                                                                                     cATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATNAAATAGAACTTGAGTGATAAAAAATAGa                                                         <  1:5064904/149‑1 (MQ=255)

                                                                                        aaGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCt                                                      <  2:9158884/149‑1 (MQ=255)

                                                                                        aaGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCt                                                      >  1:4635655/1‑149 (MQ=255)

                                                                                        aaGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTATAATAAAATAGAACTTGAGTGATAAAAAATAGAcat                                                      >  1:6878659/1‑147 (MQ=255)

                                                                                          gAAACTTTAACTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTc                                                    <  2:6792209/149‑1 (MQ=255)

                                                                                            aaCTTTACATCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTa                                                  <  2:429636/149‑1 (MQ=255)

                                                                                             aCTATACCTCAACACTGAAAAGATGGAAGGAAAGTTTAAATAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTaa                                                 <  2:444247/149‑1 (MQ=255)

                                                                                              cTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTaaa                                                <  1:4397887/149‑1 (MQ=255)

                                                                                                ttACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAgg                                              <  1:2357168/149‑1 (MQ=255)

                                                                                                  aCCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTc                                            <  1:7804414/149‑1 (MQ=255)

                                                                                                  aCCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTc                                            <  1:7187581/149‑1 (MQ=255)

                                                                                                   ccTCATCTCGGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTATGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTGCTAACGGTCt                                           >  2:3458756/1‑149 (MQ=255)

                                                                                                      cATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACATTCTAAAGGTCTA‑tt                                       >  2:1815821/1‑149 (MQ=255)

                                                                                                         ctctGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑ttttt                                    >  1:8159124/1‑149 (MQ=255)

                                                                                                                aaGATGGAAGGAAAGTATAAATAAAAAAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGAGGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTaa                             <  2:8040767/149‑1 (MQ=255)

                                                                                                                 aGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGACATCACAGAAGATGCAAAACTCTTCTAATACAATAGAACTCGCGTGATAAAACATAGACCTTCTAAACGTCTC‑ttttcttttttcc                            >  2:7131286/1‑146 (MQ=255)

                                                                                                                 aGATGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAg                            <  1:1275485/149‑1 (MQ=255)

                                                                                                                  gATGGAAAGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGa                           <  2:5242948/149‑1 (MQ=255)

                                                                                                                   aTGGAAGGAAAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGaa                          <  1:2247365/149‑1 (MQ=255)

                                                                                                                   aTGGAAGGAAAGTATAACTAAAATAAAAAAAAGGTTTTGAATAAGCTTTTGTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGaa                          <  2:4785188/149‑1 (MQ=255)

                                                                                                                     ggaaggaaAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAAc                        >  1:7448877/1‑149 (MQ=255)

                                                                                                                     ggaaggaaAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAACTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACACTTCTAATAAAATAGAAAGTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAAc                        <  2:3731633/149‑1 (MQ=255)

                                                                                                                      gaaggaaAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAACa                       <  1:5250585/149‑1 (MQ=255)

                                                                                                                      gaaggaaAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAACa                       >  1:301391/1‑149 (MQ=255)

                                                                                                                      gaaggaaAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAACa                       >  1:2098498/1‑149 (MQ=255)

                                                                                                                      gaaggaaAGTTTATCTAAAATAAAAAAAAGCTTTTGAAGAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTTATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAACa                       <  1:2001765/149‑1 (MQ=255)

                                                                                                                       aaggaaAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGGAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAACAg                      >  2:7288746/1‑149 (MQ=255)

                                                                                                                          gaaAGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACGCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAACAGaaa                   >  1:5810552/1‑149 (MQ=255)

                                                                                                                            aaGTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAAAATAAAtt                 >  2:7838959/1‑149 (MQ=255)

                                                                                                                              gTTTATCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTACTAATAAAATAGAACTTGAGTGATAAAAAATCGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAACAGAAAttaa               >  2:5745561/1‑147 (MQ=255)

                                                                                                                              gTTTATCTAAAAGAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAACAGAAATTTa               <  1:1163211/149‑1 (MQ=255)

                                                                                                                                  aTCTAAAATAAAAAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAACAGAAATTTACTTa           >  1:6496856/1‑149 (MQ=255)

                                                                                                                                     taaaataaaaAAAAGGTTTTGAATAAGCTTTTTTATTTTCAGAAATAAGAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTTAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAACAGAAATTTACTTAGAt        <  2:3561349/149‑1 (MQ=255)

                                                                                                                                     taaaataaaaAAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAACAGAAATTTACTTAGAt        <  1:3609118/149‑1 (MQ=255)

                                                                                                                                      aaaataaaaaAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGCTAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAACAGAAATTTACTTAGAtt       <  1:5388101/149‑1 (MQ=255)

                                                                                                                                      aaaataaaaaAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAg                            <  1:7943331/128‑1 (MQ=255)

                                                                                                                                      aaaataaaaaAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAACATAAATTTACTTAGAtt       >  2:8368671/1‑149 (MQ=255)

                                                                                                                                      aaaataaaaaAAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAACAGAAATTTACTTAGAtt       >  1:1003201/1‑149 (MQ=255)

                                                                                                                                       aaataaaaaaAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAACAGAAATTTACTTCGATTg      >  2:4944653/1‑149 (MQ=255)

                                                                                                                                        aataaaaaaAAGCTTTTGAATAAGCTTTTTTCTTTTCAGAAATAACCGAAGATGGAAGACTCTTCTACTGTCATCGAGCTTGAGTGATAAAACATAGACCTTGCAATGGTCTA‑TTTTTTTTTTATGAAACAGAAATTTACTTAGCTTgt     >  2:2148361/1‑148 (MQ=255)

                                                                                                                                         ataaaaaaAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAACAGAAATTTACTTAGATTgag    >  1:3277572/1‑149 (MQ=255)

                                                                                                                                         ataaaaaaAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAACAGAAATTTACTTAGATTgag    <  1:1906370/149‑1 (MQ=255)

                                                                                                                                         ataaaaaaAAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAAATCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAACAGAAATTTACTTAGATTgaa    >  2:2862505/1‑148 (MQ=255)

                                                                                                                                         ataaaaaaAAGCTTTTGAATAAACTTTTTTAATTTCAGAAATAACATAAGATAGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGAACTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAACAGAAATTTACTTAGATTgag    <  2:6532660/149‑1 (MQ=255)

                                                                                                                                          taaaaaaAAGCTTTTGAATAAGCTTTTTGATTTTCAGAATTAACAGAAGATGGAAAACGCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAACAGAAATTTACTTAGATTgaga   <  1:6631894/149‑1 (MQ=255)

                                                                                                                                           aaaaaaaaGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAACAGAAATTTACTTAGATTGAGaa  <  1:2651145/149‑1 (MQ=255)

                                                                                                                                           aaaaaaaaGCGTTTGAATAAGCATTTTTAATTTCAGAAATAACAGAAAATAGAAAAATCTTATAATAAAATAAAACTTAAGTGATAAAAAATATACCTTCTAAAAGTTTATTTTTTTTTTTAAGAAACAGAAATTTACTTAGATTgaga   <  1:1599328/149‑1 (MQ=255)

TCTTGGACAAAAGGTTCAACAGAAAGTGTTACCGTTCAATACGACAAAGCTTCTGGTCAAATTACAAGTAAATCTAAATTTAATGCATAAGAAACTTTACCTCATCTCTGAAAAGATGGAAGGAAAGTTTATCTAAAATAAAATAGAGCTTTTGAATAAGCTTTTTTATTTTCAGAAATAACAGAAGATGGAAAACTCTTCTAATAAAATAGAACTTGAGTGATAAAAAATAGACCTTCTAAAGGTCTA‑TTTTTTTTTTAAGAAACAGAAATTTACTTAGATTGAGAA  >  AM406671/1572113‑1572400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`