Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 1,588,193 T→G Q297P (CAA→CCA)  ugd ← UDP‑glucose 6‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM4066711,588,1930TG99.5% 3523.4 / NA 863Q297P (CAA→CCA) ugdUDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base G (426/432);  total (429/434)
Fisher's exact test for biased strand distribution p-value = 3.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 875 total aligned reads displayed.

CATAATCTTTCAAATCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTTGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGTATTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACC  >  AM406671/1588047‑1588338
                                                                                                                                                  |                                                                                                                                                 
cATAATCTTTCAAATCTAGTGTAGGTTCATCGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGga                                                                                                                                                 >  2:3982964/1‑149 (MQ=255)
cATAATCTTTCAAATCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGga                                                                                                                                                 <  1:842397/149‑1 (MQ=255)
 aTAATCTTTCAAATCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGgag                                                                                                                                                <  1:1007488/149‑1 (MQ=255)
   aaTCTTTCAAATCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTTACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGAt                                                                                                                                              >  2:2224479/1‑149 (MQ=255)
    aTCTTTCAAATCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGAtt                                                                                                                                             <  1:8077296/149‑1 (MQ=255)
    aTCTTTCAAATCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGAtt                                                                                                                                             >  2:293869/1‑149 (MQ=255)
     tCTTTCAAATCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGAttt                                                                                                                                            <  1:7677214/149‑1 (MQ=255)
         tCAAATCTAGTGGAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTATGAAATTATCTGACCCAGCGTTCATGACCCACCGGTCACTTCCAACAGTTTTTGGAGAGTTAGcc                                                                                                                                        >  2:3593915/1‑149 (MQ=255)
          cAAATCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCa                                                                                                                                       <  1:6463776/149‑1 (MQ=255)
             aTCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATaa                                                                                                                                    <  1:8594399/149‑1 (MQ=255)
             aTCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTACATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTATTGGAGATTTAGCCATaa                                                                                                                                    >  2:5350781/1‑149 (MQ=255)
               cTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATc                                                                                                                                  <  1:2754084/149‑1 (MQ=255)
                 aGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTg                                                                                                                                <  1:6911143/149‑1 (MQ=255)
                 aGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTg                                                                                                                                <  1:6590562/149‑1 (MQ=255)
                   tgtAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCt                                                                                                                              <  1:1836803/149‑1 (MQ=255)
                    gtAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTc                                                                                                                             >  1:7584597/1‑149 (MQ=255)
                      aGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTcaa                                                                                                                           <  2:7291268/149‑1 (MQ=255)
                         ttCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTcaacaa                                                                                                                        <  1:5154880/149‑1 (MQ=255)
                          tCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAAt                                                                                                                       >  1:3024798/1‑149 (MQ=255)
                           cATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAAtg                                                                                                                      <  1:888324/149‑1 (MQ=255)
                             tAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAAtgtg                                                                                                                    >  2:2362669/1‑149 (MQ=255)
                              aGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGc                                                                                                                   >  1:4549299/1‑149 (MQ=255)
                              aGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGc                                                                                                                   >  1:1321331/1‑149 (MQ=255)
                              aGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGc                                                                                                                   >  1:2573910/1‑149 (MQ=255)
                                  aaGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGtt                                                                                                               <  2:5615869/149‑1 (MQ=255)
                                   aGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGttt                                                                                                              >  2:2474015/1‑149 (MQ=255)
                                   aGAACCGTTAGCCCCTTATCTTGCAAGAGTTTCATCACATCAGTAATCGAACTCTGTCTGAAGTTATCTGACCCAGCTTTCATGACCAACCGGTAAACTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGttt                                                                                                              <  2:1508331/149‑1 (MQ=255)
                                     aaCCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTc                                                                                                            <  2:3192680/149‑1 (MQ=255)
                                       ccGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTg                                                                                                          <  2:1927841/149‑1 (MQ=255)
                                        cGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTgg                                                                                                         <  1:7120210/149‑1 (MQ=255)
                                         gTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTcaaca                                                                                                                         <  1:3201519/132‑1 (MQ=255)
                                         gTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGt                                                                                                        <  1:2318154/149‑1 (MQ=255)
                                             tCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCg                                                                                                    <  2:7511041/149‑1 (MQ=255)
                                             tCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCg                                                                                                    <  2:3084388/149‑1 (MQ=255)
                                             tCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCCCAACAATGTGCTGTTTTCTGGTTTCg                                                                                                    >  1:7481568/1‑149 (MQ=255)
                                              ccccTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGt                                                                                                   <  1:9024009/149‑1 (MQ=255)
                                              ccccTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGt                                                                                                   >  1:7313511/1‑149 (MQ=255)
                                                ccTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGttt                                                                                                 >  1:3587946/1‑149 (MQ=255)
                                                  ttATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTCTCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATTTGCTGTTTTCTGGTTTCGTTTgc                                                                                               >  2:8323629/1‑148 (MQ=255)
                                                  ttATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTga                                                                                               >  1:7930957/1‑148 (MQ=255)
                                                      cTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTgatttg                                                                                           >  1:2452493/1‑144 (MQ=255)
                                                      cTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTgatttg                                                                                           >  2:8515178/1‑144 (MQ=255)
                                                         gCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACa                                                                                        >  2:5075572/1‑149 (MQ=255)
                                                          cAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACaa                                                                                       >  1:151557/1‑149 (MQ=255)
                                                            agagTTTCATCACATCAACAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATa                                                                                     <  2:4527052/149‑1 (MQ=255)
                                                             gtgTTTCATCACATCAATAATCGAACTCTGTCTGAAATTAGCTCACCCAGCTTTCATGACCAACCGGTAAATTACAACAGTTTTTGGAGATTTAGACATAATCAGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAg                                                                                    <  2:6656319/147‑1 (MQ=255)
                                                             gagTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAg                                                                                    <  1:5544361/149‑1 (MQ=255)
                                                                   catcacatcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGtca                                                                              >  2:605912/1‑149 (MQ=255)
                                                                   catcacatcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGtca                                                                              >  2:8782969/1‑149 (MQ=255)
                                                                   catcacatcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGtca                                                                              <  2:6536339/149‑1 (MQ=255)
                                                                    atcacatcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGtcat                                                                             >  1:7778665/1‑149 (MQ=255)
                                                                    atcacatcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCAATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGtcat                                                                             >  2:4669809/1‑149 (MQ=255)
                                                                      cacatcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGtcatca                                                                           >  2:1698902/1‑149 (MQ=255)
                                                                        catcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAAc                                                                         >  2:5205905/1‑149 (MQ=255)
                                                                         atcaATAATCGATCTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACt                                                                        <  1:8784331/149‑1 (MQ=255)
                                                                         atcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACt                                                                        >  1:2894418/1‑149 (MQ=255)
                                                                          tcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTc                                                                       <  2:5465237/149‑1 (MQ=255)
                                                                          tcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTc                                                                       >  2:4877511/1‑149 (MQ=255)
                                                                            gataatCGAACTCTGTCTGAAAGTAGCTGACCCAGCGGGCATGACCAACCGGTAAATTCCAACAGGTTTTGGAGATTTAGCCATAATCTGCTCAACAAGGTGCTGTTTTCTGGTTTCGTTTGATTTGACAAGAGCCGTCATCAACTCCt                                                                     <  2:895067/148‑1 (MQ=255)
                                                                             ataatCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCaa                                                                          <  1:5851611/143‑1 (MQ=255)
                                                                             ataatCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCaa                                                                          >  2:5851611/1‑143 (MQ=255)
                                                                             ataatCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTg                                                                    <  1:6268095/149‑1 (MQ=255)
                                                                              taatCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGa                                                                   <  1:4224816/149‑1 (MQ=255)
                                                                               aatCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAg                                                                  <  2:2622924/149‑1 (MQ=255)
                                                                               aatCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAg                                                                  >  1:96699/1‑149 (MQ=255)
                                                                               aatCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCCGAg                                                                  >  1:4927674/1‑149 (MQ=255)
                                                                                atCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAgg                                                                 >  2:2859137/1‑149 (MQ=255)
                                                                                atCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAgg                                                                 >  2:8633551/1‑149 (MQ=255)
                                                                                 tCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGa                                                                <  2:7089968/149‑1 (MQ=255)
                                                                                 tCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGa                                                                >  1:9204793/1‑149 (MQ=255)
                                                                                  cGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGaa                                                               <  1:7393394/149‑1 (MQ=255)
                                                                                  cGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGaa                                                               <  2:8000622/149‑1 (MQ=255)
                                                                                     aCTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGc                                                            >  1:1997375/1‑149 (MQ=255)
                                                                                       tctgtctgAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTTCTCAACAATGTTCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCTTCATCAACTCCTGAGGAACGccc                                                          <  2:7447965/149‑1 (MQ=255)
                                                                                       tctgtctgAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGccc                                                          >  1:6745173/1‑149 (MQ=255)
                                                                                         tgtctgAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTc                                                        <  1:3768822/149‑1 (MQ=255)
                                                                                           tctgAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGATATTTAGCCATAATCTGCTCAACAATGTGCTGTATTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTACTGAGGAACGCCCTCAt                                                      >  2:3719638/1‑149 (MQ=255)
                                                                                            ctgAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATa                                                     >  2:1130297/1‑149 (MQ=255)
                                                                                            ctgAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATa                                                     >  1:8279584/1‑149 (MQ=255)
                                                                                              gAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGGTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATATCCGTCATCAACTCCTGAGGAACGCCCTCATAAc                                                   <  2:1295182/149‑1 (MQ=255)
                                                                                                  ttATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGc                                               <  2:4432984/149‑1 (MQ=255)
                                                                                                           ccAGCTTACATGACCAACCGGTAACTGCCAACAAATATTGCAGACTCAGCAATCATCTGCCCAAAAAAGTGCTGTTTCCTGGTTTCGATTGATATCACAATAGCCGTCATCAACTCCTGAGGAACAACCACTAAACTGTCCCAAAgcta                                      >  2:4123060/1‑148 (MQ=255)
                                                                                                            cAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGATGTTTTCTGGTTTCTTTTGATTTGACAATAGCCATCATCAAATCATGAGGAACGCACTCATAACTAGCCAAAAactgg                                     >  2:6262962/1‑148 (MQ=255)
                                                                                                             aGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAgctgct                                    <  1:1696268/149‑1 (MQ=255)
                                                                                                               cTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCttt                                  >  1:5728457/1‑149 (MQ=255)
                                                                                                                 ttCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCCAAAGCTGCTTTGa                                >  2:9126458/1‑149 (MQ=255)
                                                                                                                    aTGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTc                             <  1:6058272/149‑1 (MQ=255)
                                                                                                                    aTGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTc                             <  1:3502505/149‑1 (MQ=255)
                                                                                                                      gACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCtt                           <  1:4404374/149‑1 (MQ=255)
                                                                                                                        ccAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTc                         <  2:7811747/149‑1 (MQ=255)
                                                                                                                         cAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGGGTCTTTCg                        >  1:5305598/1‑149 (MQ=255)
                                                                                                                          aaCCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGTCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCgg                       <  1:5417730/149‑1 (MQ=255)
                                                                                                                           aCCGGTAAATTCCAACAGTTTTTTTAGTTTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGc                      >  2:212252/1‑149 (MQ=255)
                                                                                                                           aCCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGc                      <  1:1082797/149‑1 (MQ=255)
                                                                                                                            ccGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCa                     >  1:3983010/1‑149 (MQ=255)
                                                                                                                              ggTAAATTACAAGAGTTTTTGCAGATTTAGCGATAATGTGCTAAACAATGTGATGTTTTCTGATTTAGTTTGATTTGACATTAGCCGTCATCAACTCCTGACGATCGTCCTAATAACTGGCGAATGTCTGCTTTAGGTCTTTCGGCaaa                   <  2:68144/149‑1 (MQ=255)
                                                                                                                                tAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAACAGCTGCTTTGAGTCTTTCGGCAACCa                 >  1:4747752/1‑149 (MQ=255)
                                                                                                                                         aCAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAaccacc        >  1:2157331/1‑149 (MQ=255)
                                                                                                                                          cAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAAccacca       <  2:3359793/149‑1 (MQ=255)
                                                                                                                                             tttttgGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATaa    <  2:8217828/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CATAATCTTTCAAATCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTTGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGTATTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACC  >  AM406671/1588047‑1588338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑