Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 447,854 G→A A98T (GCT→ACT)  llmg_0454 → putative beta‑glucosides‑specific IIBC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM406671447,8540GA99.8% 4245.9 / NA 1090A98T (GCT→ACT) llmg_0454putative beta‑glucosides‑specific IIBC component
Reads supporting (aligned to +/- strand):  ref base G (1/1);  major base A (509/577);  minor base C (1/1);  total (511/579)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1103 total aligned reads displayed.

TACCTGGTGTCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTGCTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGATTATTAAGTATCTT  >  AM406671/447708‑447999
                                                                                                                                                  |                                                                                                                                                 
  ccTGGTGTCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTtactgc                                                                                                                                               >  2:7251857/1‑149 (MQ=255)
   cTGGTGTCGCTGGAGGTGGTTGTAACTCCAATATGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCa                                                                                                                                              >  2:4329636/1‑149 (MQ=255)
     ggTGTCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAAc                                                                                                                                            <  1:7661475/149‑1 (MQ=255)
      gtgtCGCTGGAGCTGTTTATAACTCGAACTTGAATCGATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACa                                                                                                                                           <  1:564344/149‑1 (MQ=255)
       tgtCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAg                                                                                                                                          <  1:8764263/149‑1 (MQ=255)
            cTGGAGCTGTTTATAACTCAAACTTGAAACAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCg                                                                                                                                     <  1:1232799/149‑1 (MQ=255)
              ggAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGc                                                                                                                                   <  2:817866/149‑1 (MQ=255)
              ggAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGc                                                                                                                                   <  2:7652751/149‑1 (MQ=255)
              ggAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGc                                                                                                                                   >  2:8429524/1‑149 (MQ=255)
                 gCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAg                                                                                                                                <  1:4790815/149‑1 (MQ=255)
                  cTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGt                                                                                                                               <  2:9226903/149‑1 (MQ=255)
                     tttATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGt                                                                                                                            <  2:4650103/149‑1 (MQ=255)
                       tataACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTaa                                                                                                                          >  2:1258782/1‑149 (MQ=255)
                       tataACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTaa                                                                                                                          >  2:7115013/1‑149 (MQ=255)
                        ataACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTaaa                                                                                                                         >  2:2234644/1‑149 (MQ=255)
                        ataACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTaaa                                                                                                                         >  1:1891683/1‑149 (MQ=255)
                        ataACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTaaa                                                                                                                         <  1:5288767/149‑1 (MQ=255)
                         taACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTaaaa                                                                                                                        <  2:6000827/149‑1 (MQ=255)
                          aaCTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTaaaaa                                                                                                                       >  2:8326010/1‑149 (MQ=255)
                             tCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACa                                                                                                                    >  1:3083012/1‑149 (MQ=255)
                              cAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACaa                                                                                                                   >  2:6955822/1‑149 (MQ=255)
                              cAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACaa                                                                                                                   >  2:4235869/1‑149 (MQ=255)
                               aaaCTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACaaa                                                                                                                  <  1:797444/149‑1 (MQ=255)
                               aaaCTTCAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACaaa                                                                                                                  <  2:2138643/149‑1 (MQ=255)
                                aaCTTGAATCAATATCAAGTAGCTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGTAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACaaaa                                                                                                                 >  2:1977540/1‑149 (MQ=255)
                                   ttGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATcc                                                                                                              <  1:996681/149‑1 (MQ=255)
                                   ttGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATcc                                                                                                              >  1:2149236/1‑149 (MQ=255)
                                    tGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCa                                                                                                             <  1:2324611/149‑1 (MQ=255)
                                    tGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCa                                                                                                             <  2:7778203/149‑1 (MQ=255)
                                    tGAATCAATATCAAGTAGTTATTGGACAACCTTTAGAAGCTGTTTATGACGAGGTTGTTGGACAGCTTAGAGATTCAGTTGTTGATGACGATGCAACGGCGCACGCAGTTACTGCAACAGCACTGGCTAGTGGTAAAAAACAAAACCCa                                                                                                             >  2:1454963/1‑149 (MQ=255)
                                         cAATATGAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGGTTGGAGATTCAATGGTTGATGAATATGCAACGGCGCAAGCACTTACCGCAACAGCACCGTCGAGTGGTAAAAAACAAAATCCAATTGt                                                                                                        <  2:570170/149‑1 (MQ=255)
                                         cAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGt                                                                                                        >  2:4909170/1‑149 (MQ=255)
                                            tatCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCa                                                                                                     <  1:6786209/149‑1 (MQ=255)
                                             atCAAGTAGGTAGTGGACAAGCTGTATAAGATGTTTATGACGAGGTTTTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCAt                                                                                                    <  1:713951/149‑1 (MQ=255)
                                              tCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGAGGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATg                                                                                                   >  2:5131161/1‑149 (MQ=255)
                                              tCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATg                                                                                                   <  1:8429524/149‑1 (MQ=255)
                                                 agtagtTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCtt                                                                                                <  1:3314509/149‑1 (MQ=255)
                                                 agtagtTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCtt                                                                                                >  1:8598697/1‑149 (MQ=255)
                                                  gtagtTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCttt                                                                                               <  1:4098574/149‑1 (MQ=255)
                                                   tagtTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTc                                                                                              >  1:9014209/1‑149 (MQ=255)
                                                     gtTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCa                                                                                            <  1:3629826/149‑1 (MQ=255)
                                                     gtTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCa                                                                                            >  2:8578827/1‑149 (MQ=255)
                                                     gtTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCa                                                                                            <  1:660642/149‑1 (MQ=255)
                                                          tGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTgg                                                                                       >  1:8294241/1‑149 (MQ=255)
                                                          tGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTgg                                                                                       >  1:4404666/1‑149 (MQ=255)
                                                             ccAAGCTGTAGAAGATGTTTATGACGTGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAATCACTTACTGCAACAGGAGCGGCTAGTGGTAAAATACAAAATGCAATTGTTCATGCTTTGCTAGTGGTTa                                                                                    >  2:2411966/2‑149 (MQ=255)
                                                                aGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTg                                                                                 >  1:4649007/1‑149 (MQ=255)
                                                                  cTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTggg                                                                               <  2:6229712/149‑1 (MQ=255)
                                                                     tAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACa                                                                            <  2:3021209/149‑1 (MQ=255)
                                                                     tAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACa                                                                            <  1:8146326/149‑1 (MQ=255)
                                                                      agaagaTGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACaa                                                                           <  1:5453535/149‑1 (MQ=255)
                                                                      agaagaTGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACaa                                                                           <  2:4113671/149‑1 (MQ=255)
                                                                            tGTTTATGACGAGGTTGTTGAACAGCTTGGAGATGGAGTTGTTGATGAAGATGCAACGGAGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTACAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTCTCGGGACACTTACAg                                                                     >  2:6457898/1‑149 (MQ=255)
                                                                             gTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAgg                                                                    >  2:8678079/1‑149 (MQ=255)
                                                                              tttATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGt                                                                   <  1:4228487/149‑1 (MQ=255)
                                                                              tttATGACGAGGTTGTTGAACAGCTCGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGt                                                                   >  2:4987668/1‑149 (MQ=255)
                                                                                 aTGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCg                                                                <  2:6780825/149‑1 (MQ=255)
                                                                                    aCGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCgatg                                                             <  2:7940374/149‑1 (MQ=255)
                                                                                      gAGGTTGTTGACCAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCgatgat                                                           <  1:1026703/149‑1 (MQ=255)
                                                                                       aGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGAtt                                                          >  1:2717143/1‑149 (MQ=255)
                                                                                         gttgttGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTcc                                                        <  1:1541298/149‑1 (MQ=255)
                                                                                         gttgttGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTcc                                                        >  1:7555547/1‑149 (MQ=255)
                                                                                           tgttgAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCaa                                                      <  1:7836565/149‑1 (MQ=255)
                                                                                              tgAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAatta                                                   <  2:7585209/149‑1 (MQ=255)
                                                                                               gAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAattat                                                  <  1:1653471/149‑1 (MQ=255)
                                                                                                aaCAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACGGGCTAGTGGTAAAAAACAAAATCCCATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAattatt                                                 >  2:3913075/1‑149 (MQ=255)
                                                                                                aaCAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTAGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAattatt                                                 <  2:2686295/149‑1 (MQ=255)
                                                                                                 aCAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTg                                                >  2:6565105/1‑149 (MQ=255)
                                                                                                 aCAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTg                                                >  1:4509445/1‑149 (MQ=255)
                                                                                                  cAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGAGACAATTACAGGTTCGATGATTCCAATTATTgg                                               <  2:5325777/149‑1 (MQ=255)
                                                                                                   aGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGt                                              >  1:5998678/1‑149 (MQ=255)
                                                                                                      ttGGAGATTGAGTTGTGGGTGCAGATGAAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTa                                           <  2:451340/149‑1 (MQ=255)
                                                                                                       tGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTAc                                          <  1:5767561/149‑1 (MQ=255)
                                                                                                        ggAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACt                                         >  1:3805702/1‑149 (MQ=255)
                                                                                                         gagaTTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACtt                                        <  1:6384978/149‑1 (MQ=255)
                                                                                                             ttCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCgg                                    <  1:5852668/149‑1 (MQ=255)
                                                                                                             ttCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCgg                                    <  1:1391940/149‑1 (MQ=255)
                                                                                                                 gttgttAATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGTTTTACTTGCGGCtgg                                <  2:5881695/149‑1 (MQ=255)
                                                                                                                     ttgATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTggg                            >  1:9151476/1‑149 (MQ=255)
                                                                                                                      tgatgaAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGga                           >  1:3512435/1‑149 (MQ=255)
                                                                                                                        atgaAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGgatg                         <  2:4780116/149‑1 (MQ=255)
                                                                                                                        atgaAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGgatg                         <  2:900979/149‑1 (MQ=255)
                                                                                                                         tgaAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGgatga                        <  2:2879714/149‑1 (MQ=255)
                                                                                                                          gaAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGgatgat                       <  1:6234216/149‑1 (MQ=255)
                                                                                                                           aaGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCCTGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGAtt                      <  1:2584322/149‑1 (MQ=255)
                                                                                                                           aaGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGAtt                      >  1:1161449/1‑149 (MQ=255)
                                                                                                                             gATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTaa                    <  2:3516330/149‑1 (MQ=255)
                                                                                                                                gCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATgg                 <  2:175243/149‑1 (MQ=255)
                                                                                                                                  aaCGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGat               <  1:2043364/149‑1 (MQ=255)
                                                                                                                                    cGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGatta             >  1:8898498/1‑149 (MQ=255)
                                                                                                                                    cGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGatta             <  1:4981079/149‑1 (MQ=255)
                                                                                                                                      gcgcAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGattatt           <  1:696618/149‑1 (MQ=255)
                                                                                                                                      gagcAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGattatt           <  1:2893425/147‑1 (MQ=255)
                                                                                                                                       cgcAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGAttatta          <  2:369423/149‑1 (MQ=255)
                                                                                                                                        gcAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGATTATTaa         <  1:7336859/149‑1 (MQ=255)
                                                                                                                                         aaaGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGATTATTAAg        <  2:1132571/148‑1 (MQ=255)
                                                                                                                                          aaGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGATTATTAAGt       <  2:3137968/149‑1 (MQ=255)
                                                                                                                                            gCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGATTATTAAGTAt     >  1:2441264/1‑149 (MQ=255)
                                                                                                                                             cACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGATTATTAAGTATc    <  2:3396815/149‑1 (MQ=255)
                                                                                                                                              aCTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGATTATTAAGTATCt   <  2:7421979/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TACCTGGTGTCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTGCTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGATTATTAAGTATCTT  >  AM406671/447708‑447999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑