Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 1,610,761 T→C I430V (ATT→GTT)  llmg_1634 ← putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM4066711,610,7610TC99.7% 4106.0 / NA 990I430V (ATT→GTT) llmg_1634putative membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (480/507);  minor base A (2/1);  total (482/508)
Fisher's exact test for biased strand distribution p-value = 6.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1017 total aligned reads displayed.

TCCCAAAAGCGTAGCAAAGAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAATATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGATAGAAATTCAACTTCATGGGT  >  AM406671/1610615‑1610906
                                                                                                                                                  |                                                                                                                                                 
 cccAAAAGCGTAGCAAAGAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACAtt                                                                                                                                                >  2:3136865/1‑149 (MQ=255)
       aGCGTAGCAAAGAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAAt                                                                                                                                          >  1:4994933/1‑149 (MQ=255)
        gCGTAGCAAAGAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAAtt                                                                                                                                         >  1:4291994/1‑149 (MQ=255)
        gCGTAGCAAAGAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAAtt                                                                                                                                         >  1:3995660/1‑149 (MQ=255)
        gCGTAGCAAAGAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAAtt                                                                                                                                         <  1:3260095/149‑1 (MQ=255)
         cGTAGCAAAGAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTa                                                                                                                                        >  2:7122340/1‑149 (MQ=255)
          gTAGCAAAGAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTaa                                                                                                                                       <  1:7759751/149‑1 (MQ=255)
           tAGCAAAGAAAATGATAAAAACCAGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAAc                                                                                                                                      >  2:1329009/1‑149 (MQ=255)
             gCAAAGAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAg                                                                                                                                    >  1:1006531/1‑149 (MQ=255)
               aaaGAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGcc                                                                                                                                  <  2:1708228/149‑1 (MQ=255)
                  gAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCaa                                                                                                                               <  1:7676183/149‑1 (MQ=255)
                  gAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCaa                                                                                                                               >  1:2817470/1‑149 (MQ=255)
                   aaaaTGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCaaa                                                                                                                              >  2:4322047/1‑149 (MQ=255)
                      aTGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAagag                                                                                                                           >  2:7010049/1‑149 (MQ=255)
                      aTGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAagag                                                                                                                           <  1:5336323/149‑1 (MQ=255)
                      aTGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCACAAagag                                                                                                                           >  2:1179860/1‑149 (MQ=255)
                           aaaaaCCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAgg                                                                                                                      >  2:5413519/1‑149 (MQ=255)
                            aaaaCCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGt                                                                                                                     <  2:8576074/149‑1 (MQ=255)
                              aaCCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTaa                                                                                                                   <  1:1836591/149‑1 (MQ=255)
                               aCCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAAc                                                                                                                  >  1:3689480/1‑149 (MQ=255)
                                 ccGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAg                                                                                                                >  1:5730532/1‑149 (MQ=255)
                                   gAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAggg                                                                                                              >  2:3760756/1‑149 (MQ=255)
                                    aaaTCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGa                                                                                                             >  1:5553963/1‑149 (MQ=255)
                                     aaTCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGAc                                                                                                            <  1:4563927/149‑1 (MQ=255)
                                       tCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACtt                                                                                                          <  1:2659123/149‑1 (MQ=255)
                                        cccccAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACttt                                                                                                         <  2:6828299/149‑1 (MQ=255)
                                        cccccAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAAACAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACttt                                                                                                         <  1:6504627/149‑1 (MQ=255)
                                           ccAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAAt                                                                                                                                          >  2:9114519/1‑113 (MQ=255)
                                           ccAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAACTGAAATCAAATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAg                                                                                                      <  1:2250875/149‑1 (MQ=255)
                                             aGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTa                                                                                                    >  1:2017212/1‑149 (MQ=255)
                                              gAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTaa                                                                                                   >  1:2441166/1‑149 (MQ=255)
                                               aaCCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTaaa                                                                                                  >  1:1270494/1‑149 (MQ=255)
                                               aaCCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTaaa                                                                                                  >  2:1039079/1‑149 (MQ=255)
                                               aaCCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTaaa                                                                                                  <  2:4150032/149‑1 (MQ=255)
                                               aaCCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTaaa                                                                                                  <  1:4836895/149‑1 (MQ=255)
                                                aCCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATCTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTaaaa                                                                                                 >  2:6236178/1‑149 (MQ=255)
                                                  ctctTGTAAAACGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATa                                                                                               >  2:4782227/1‑149 (MQ=255)
                                                    ctTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATAcc                                                                                             >  2:7553205/1‑149 (MQ=255)
                                                    ctTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAAGCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATAcc                                                                                             <  2:2681797/149‑1 (MQ=255)
                                                    ctTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAAACAAATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATAcc                                                                                             <  2:4974194/149‑1 (MQ=255)
                                                     ttGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCa                                                                                            <  1:1162969/149‑1 (MQ=255)
                                                      tGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGAc                                                                                                            >  2:587712/1‑132 (MQ=255)
                                                         aaaaaGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCa                                                                                        <  2:8817413/149‑1 (MQ=255)
                                                          aaaaGTTGAGTCAACGCAGTCCAATTCAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCAc                                                                                       <  1:5143380/149‑1 (MQ=255)
                                                            aaGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTa                                                                                     <  2:6559669/149‑1 (MQ=255)
                                                              gTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTaaa                                                                                   <  1:9070587/149‑1 (MQ=255)
                                                              gTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTaaa                                                                                   <  1:6208202/149‑1 (MQ=255)
                                                               ttGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACTTTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAaag                                                                                  <  2:6122650/149‑1 (MQ=255)
                                                                    tCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAaagataag                                                                             <  2:7700690/149‑1 (MQ=255)
                                                                    tCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAaagataag                                                                             <  1:7259023/149‑1 (MQ=255)
                                                                     cAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAaagataaga                                                                            <  1:1486343/149‑1 (MQ=255)
                                                                        cGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTa                                                                         <  1:1714020/149‑1 (MQ=255)
                                                                         gCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACACAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTaa                                                                        <  1:6607447/149‑1 (MQ=255)
                                                                              ccAATTTAAATTTAACAGATGCCAAACTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCaa                                                                   <  1:7008113/149‑1 (MQ=255)
                                                                                aatttaaatttaAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGa                                                                 <  2:6315385/149‑1 (MQ=255)
                                                                                   ttaaatttaaGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACt                                                              <  1:4167183/149‑1 (MQ=255)
                                                                                   ttaaatttaaGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACt                                                              >  1:5958695/1‑149 (MQ=255)
                                                                                    taaatttaaGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTa                                                             <  2:3995660/149‑1 (MQ=255)
                                                                                     aaatttaaGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAg                                                            <  1:1102784/149‑1 (MQ=255)
                                                                                       atttaaGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGtt                                                          <  1:7932266/149‑1 (MQ=255)
                                                                                       atttaaGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGtt                                                          <  1:756157/149‑1 (MQ=255)
                                                                                        tttaaGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTg                                                         <  1:586693/149‑1 (MQ=255)
                                                                                         ttaaGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGa                                                        <  1:3909869/149‑1 (MQ=255)
                                                                                         ttaaGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGa                                                        <  2:1410281/149‑1 (MQ=255)
                                                                                          taaGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGaa                                                       <  2:231280/149‑1 (MQ=255)
                                                                                              agaTGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATaaa                                                   >  1:3050349/1‑149 (MQ=255)
                                                                                                 tGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATaaaaaa                                                >  1:3510129/1‑149 (MQ=255)
                                                                                                    cAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGc                                             <  2:8919908/149‑1 (MQ=255)
                                                                                                      aTCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCaa                                           <  1:2555202/149‑1 (MQ=255)
                                                                                                      aTCTCTGACAAAATAATGAAGTGAAAACAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCaa                                           <  2:836432/149‑1 (MQ=255)
                                                                                                       tctcTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCaaa                                          <  1:8830777/149‑1 (MQ=255)
                                                                                                          ctGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATg                                       >  1:7400603/1‑149 (MQ=255)
                                                                                                           tGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGa                                      >  2:817496/1‑149 (MQ=255)
                                                                                                           tGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGa                                      <  1:217751/149‑1 (MQ=255)
                                                                                                            gACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGaa                                     >  2:3279262/1‑149 (MQ=255)
                                                                                                               aaaaTAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGGAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATAAAGAACTAGTTGAATAAAAAAGGCAAAATGAAAtt                                  >  2:5816632/1‑149 (MQ=255)
                                                                                                                  ataatGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCa                               >  2:8431378/1‑149 (MQ=255)
                                                                                                                   taatGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCAc                              <  2:2812243/149‑1 (MQ=255)
                                                                                                                    aatGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCAct                             >  1:7151618/1‑149 (MQ=255)
                                                                                                                       gAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTg                          <  2:4495586/149‑1 (MQ=255)
                                                                                                                        aaGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTga                         >  2:5163606/1‑149 (MQ=255)
                                                                                                                        aaGAGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAAAAAAAAAAAAAAAAAAGGCAAAATGAAATTTCACTCTga                         <  2:5520241/149‑1 (MQ=255)
                                                                                                                         aGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTgag                        >  2:1890434/1‑149 (MQ=255)
                                                                                                                          gTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTgaga                       >  1:6789855/1‑149 (MQ=255)
                                                                                                                          gTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTgaga                       >  2:2490214/1‑149 (MQ=255)
                                                                                                                           tGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGAt                      >  2:2079938/1‑149 (MQ=255)
                                                                                                                           tGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGAt                      <  1:8437255/149‑1 (MQ=255)
                                                                                                                             aaaTCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGATAg                    >  1:6377368/1‑149 (MQ=255)
                                                                                                                              aaTCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGATAGa                   <  2:8025750/149‑1 (MQ=255)
                                                                                                                               aTCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGATAGaa                  <  1:4420061/149‑1 (MQ=255)
                                                                                                                                tCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGATAGaaa                 <  2:180363/149‑1 (MQ=255)
                                                                                                                                 cAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGATAGAAAt                <  2:1555394/149‑1 (MQ=255)
                                                                                                                                    atatACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGATAGAAATTCa             <  1:9161236/149‑1 (MQ=255)
                                                                                                                                    atatACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGATAGAAATTCa             <  2:3510130/149‑1 (MQ=255)
                                                                                                                                    atatACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAACATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGATAGAAATTCa             <  1:4743289/149‑1 (MQ=255)
                                                                                                                                      ataCCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGATAGAAATTCAAc           <  1:8607058/149‑1 (MQ=255)
                                                                                                                                         ccccAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGATAGAAATTCAACTTc        <  2:7895495/149‑1 (MQ=255)
                                                                                                                                           ccAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAACTTCACTCTGAGATAGAAATTCAACTTCAt      <  2:5674510/149‑1 (MQ=255)
                                                                                                                                             aataacATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGATAGAAATTCAACTTCATgg    <  2:7356423/149‑1 (MQ=255)
                                                                                                                                              ataacaTTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGATAGAAATTCAACTTCATggg   >  1:8162179/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TCCCAAAAGCGTAGCAAAGAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAATATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGATAGAAATTCAACTTCATGGGT  >  AM406671/1610615‑1610906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑