BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,655,780	G→A	L69F (CTT→TTT)	ftsL ←	cell division protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,655,780	0	G	A	99.5%	3589.9 / NA	943	L69F (CTT→TTT)	ftsL	cell division protein
Reads supporting (aligned to +/- strand): ref base G (1/4); new base A (483/452); total (486/457)
Fisher's exact test for biased strand distribution p-value = 2.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 944 total aligned reads displayed.

ATTTGCTTTTAAACTTGCTTTGTTTGCTGCATCTAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAGCGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAACTTACTTGCTAGAACTTCTGGCGCTAAAGTCTCAGTATCAATAAGA  >  AM406671/1655634‑1655925

aTTTGCTTTTAAACTTGCTTTGTTTGCTGCATCTAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACg                                                                                                                                                 >  2:6307128/1‑149 (MQ=255)

  ttGCTTTTAAACTTGCTTTGTTTGCTGCATCTAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGtt                                                                                                                                               <  2:2869577/149‑1 (MQ=255)

   tGCTTTTAAACTTGCTTTGTTTGCTGCATCTAACACTCTATTACCAGACGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGttt                                                                                                                                              >  1:5029759/1‑149 (MQ=255)

    gCTTTTAAACTTGCTTTGTTTGCTGCATCTAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCTCTTGGACTTCAAACGtttt                                                                                                                                             >  2:3482582/1‑149 (MQ=255)

    gCTTTTAAACTTGCTTTGTTTGCTGCATCTAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGtttt                                                                                                                                             <  1:5366455/149‑1 (MQ=255)

        ttAAACTTGCTTTGTTTGCTGCATCTAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGtt                                                                                                                                         <  1:4677386/149‑1 (MQ=255)

         tAAGCTTTCTTTGGTTGCTTGATACAGCACTTTAATACTAAGTGTTAGAACTAAAACTTGTTGGTCATATTCCGATTTACGTGTGGCTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGAATTCAAACGTTTTGGttt                                                                                                                                        <  2:869553/149‑1 (MQ=255)

          aaaCTTGCTTTGTTTGCTGCATCTAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGtttt                                                                                                                                       >  1:7923131/1‑149 (MQ=255)

          aaaCTTGCTTTGTTTGCTGCATCTAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGtttt                                                                                                                                       >  1:9220315/1‑149 (MQ=255)

           aaCTTGCTTTGTTTGCTGCATCTAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGttttt                                                                                                                                      >  2:9209460/1‑149 (MQ=255)

              ttGCTTTGTTTGCTGCATCTAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACa                                                                                                                                   >  2:3678429/1‑149 (MQ=255)

                gCTTTGTTTGCTGCATCTAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATa                                                                                                                                 >  2:451773/1‑149 (MQ=255)

                 cTTTGTTTGCTGCATCTAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATaa                                                                                                                                <  1:2007212/149‑1 (MQ=255)

                  tttgtttgCTGCATCTAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATaaa                                                                                                                               >  1:2632051/1‑149 (MQ=255)

                   ttgtttgCTGCATCTAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGttt                                                                                                                                        <  1:6490581/139‑1 (MQ=255)

                   ttgtttgCTGCATCTAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGttt                                                                                                                                        >  2:6490581/1‑139 (MQ=255)

                   ttgtttgCTGCATCTAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAag                                                                                                                              <  1:3239496/149‑1 (MQ=255)

                    tgtttgCTGCATCTAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAagt                                                                                                                             >  2:883576/1‑149 (MQ=255)

                     gtttgCTGCATCTAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAagta                                                                                                                            >  2:6084848/1‑149 (MQ=255)

                     gtttgCTGCATCTAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAagta                                                                                                                            <  1:1220383/149‑1 (MQ=255)

                       ttgCTGCATCTAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAagtagt                                                                                                                          <  2:8146609/149‑1 (MQ=255)

                       ttgCTGCATCTAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAagtagt                                                                                                                          <  1:4864860/149‑1 (MQ=255)

                          ctgcATCTAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCa                                                                                                                       <  1:4300683/149‑1 (MQ=255)

                          ctgcATCTAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAAAGTTTTGGTTTTTACATAAAGTAGTCCa                                                                                                                       >  1:2815985/1‑149 (MQ=255)

                            gcATCTAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAAc                                                                                                                     >  2:5573819/1‑149 (MQ=255)

                                 tAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCa                                                                                                                >  1:3475015/1‑149 (MQ=255)

                                   acacTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAac                                                                                                              >  2:1005190/1‑149 (MQ=255)

                                   acacTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAac                                                                                                              >  2:1587937/1‑149 (MQ=255)

                                    cacTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAaca                                                                                                             >  1:3951110/1‑149 (MQ=255)

                                    cacTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAaca                                                                                                             <  2:1386113/149‑1 (MQ=255)

                                     acTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAacac                                                                                                            >  2:3805947/1‑149 (MQ=255)

                                        ctATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTCTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTaa                                                                                                         >  1:7371713/1‑149 (MQ=255)

                                         tATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTaaa                                                                                                        <  2:7246997/149‑1 (MQ=255)

                                           ttACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAagc                                                                                                      >  1:9021026/1‑149 (MQ=255)

                                            tACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAagca                                                                                                     >  1:6567789/1‑149 (MQ=255)

                                                aGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTGCCTTGGACTTCACACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCaa                                                                                                 >  2:9019369/1‑149 (MQ=255)

                                                aGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCaa                                                                                                 <  1:4140083/149‑1 (MQ=255)

                                                 gATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAAc                                                                                                >  1:7512553/1‑149 (MQ=255)

                                                 gATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAAc                                                                                                >  2:591111/1‑149 (MQ=255)

                                                     ttAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTAcc                                                                                            <  2:8063354/149‑1 (MQ=255)

                                                      tAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCa                                                                                           <  2:7887910/149‑1 (MQ=255)

                                                      tAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCa                                                                                           <  2:4675328/149‑1 (MQ=255)

                                                       aGATCTAGAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTGGAGAAATTTTTGTTCTCACATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCTACACTAAAGCAGCAACTACCAt                                                                                          <  2:7011787/149‑1 (MQ=255)

                                                       aGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCAt                                                                                          >  1:3110955/1‑149 (MQ=255)

                                                         aTCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCg                                                                                        >  2:4000365/1‑149 (MQ=255)

                                                           cTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTc                                                                                      <  1:6329817/149‑1 (MQ=255)

                                                             aaaaTTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCaa                                                                                    <  1:6745986/149‑1 (MQ=255)

                                                                 tttGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAg                                                                                >  2:410292/1‑149 (MQ=255)

                                                                 tttGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAg                                                                                >  1:5175162/1‑149 (MQ=255)

                                                                  ttgttgGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGa                                                                               >  1:61460/1‑149 (MQ=255)

                                                                      tgGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATAc                                                                           >  1:8425222/1‑149 (MQ=255)

                                                                        gTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACtt                                                                         <  2:6838124/149‑1 (MQ=255)

                                                                        gTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACtt                                                                         <  1:1005190/149‑1 (MQ=255)

                                                                        gTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACtt                                                                         >  1:3742136/1‑149 (MQ=255)

                                                                          cATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACtttt                                                                       <  1:7017366/149‑1 (MQ=255)

                                                                            tatTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTc                                                                     >  1:1534521/1‑149 (MQ=255)

                                                                            tatTCGGATTTACGTGTGGTTTCTTCAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTc                                                                     <  1:3853282/149‑1 (MQ=255)

                                                                             atTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTcc                                                                    >  2:7490215/1‑149 (MQ=255)

                                                                               tCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCcac                                                                  <  1:8253658/149‑1 (MQ=255)

                                                                               tCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCcac                                                                  >  1:2484587/1‑149 (MQ=255)

                                                                                    tttACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCAcaccac                                                             <  2:2092772/149‑1 (MQ=255)

                                                                                       aCGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTaa                                                          >  1:2128173/1‑149 (MQ=255)

                                                                                        cGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTaaa                                                         >  1:56016/1‑149 (MQ=255)

                                                                                         gtgtGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAAt                                                        <  1:8814137/149‑1 (MQ=255)

                                                                                           gtgGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAAttt                                                      <  2:4992695/149‑1 (MQ=255)

                                                                                           gtgGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAAttt                                                      <  2:301563/149‑1 (MQ=255)

                                                                                             ggTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAAttttt                                                    <  2:2349020/149‑1 (MQ=255)

                                                                                               tttCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTa                                                  <  1:8564880/149‑1 (MQ=255)

                                                                                               tttCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTa                                                  <  2:6705143/149‑1 (MQ=255)

                                                                                               tttCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTa                                                  <  1:2390644/149‑1 (MQ=255)

                                                                                                     gagaAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAactta                                            >  2:7657252/1‑149 (MQ=255)

                                                                                                     gagaAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAactta                                            >  1:6012995/1‑149 (MQ=255)

                                                                                                     gagaAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAactta                                            >  2:3283909/1‑149 (MQ=255)

                                                                                                     gagaAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAactta                                            >  2:1247365/1‑149 (MQ=255)

                                                                                                      agaAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAacttac                                           >  2:1911348/1‑149 (MQ=255)

                                                                                                      agaAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAacttac                                           <  1:4572854/149‑1 (MQ=255)

                                                                                                       gaAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAacttact                                          <  1:8082507/149‑1 (MQ=255)

                                                                                                         aaTTTTTGTTCTCAAATCCGTTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTACTCCAACAGCCAACACTAAAGCAGCAACTACCATCGGCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAACGTACTTg                                        <  2:4465406/149‑1 (MQ=255)

                                                                                                           tttttGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTAGTTTTTACATAAAGTAGTCCACCAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAACTTACTTGCt                                      <  1:446083/149‑1 (MQ=255)

                                                                                                             tttGTTATCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACGCTAGAGCGGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAACTTACTTGCTAg                                    <  2:5779594/149‑1 (MQ=255)

                                                                                                              ttGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAACTTACTTGCTAGa                                   <  2:4847915/149‑1 (MQ=255)

                                                                                                                 ttCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAACTTACTTGCTAGAACt                                <  1:1808701/149‑1 (MQ=255)

                                                                                                                 ttCTCAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAACTTACTTGCTAGAACt                                >  2:4328521/1‑149 (MQ=255)

                                                                                                                  tctcAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAACTTACTTGCTAGAACtt                               >  2:1676287/1‑149 (MQ=255)

                                                                                                                     cAAATCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAACTTACTTGCTAGAACTTCTg                            >  1:1385832/1‑149 (MQ=255)

                                                                                                                        aTCCATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAACTTACTTGCTAGAACTTCTGgcg                         <  1:5471732/149‑1 (MQ=255)

                                                                                                                          ccATTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAACTTACTTGCTAGAACTTCTGGCGCt                       >  2:2472618/1‑149 (MQ=255)

                                                                                                                            aTTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAACTTACTTGCTAGAACTTCTGGCGCTaa                     >  2:5239080/1‑149 (MQ=255)

                                                                                                                            aTTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAACTTACTTGCTAGAACTTCTGGCGCTaa                     <  1:7765280/149‑1 (MQ=255)

                                                                                                                            aTTGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAACTTACTTGCTAGAACTTCTGGCGCTaa                     <  2:2627712/149‑1 (MQ=255)

                                                                                                                              tGTATTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAACTTACTTGCTAGAACTTCTGGCGCTAAAg                   <  1:3655202/149‑1 (MQ=255)

                                                                                                                               gTATTCCCTTGGACTTCAAACGTTTTGGATTTTACATAAAGTAGTCCAACAGCGAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAACTTACTTGCTAGAACTTCTGGCGCTAAAGt                  >  2:5342517/1‑149 (MQ=255)

                                                                                                                                 aTTCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAACTTACTTGCTAGAACTTCTGGCGCTAAAGtct                <  2:7139293/149‑1 (MQ=255)

                                                                                                                                  ttCCCTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAACTTACTTGCTAGAACTTCTGGCGCTAAAGtctc               <  1:340745/149‑1 (MQ=255)

                                                                                                                                      cTTGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAACTTACTTGCTAGAACTTCTGGCGCTAAAGTCTCAGTa           >  1:5713402/1‑149 (MQ=255)

                                                                                                                                       ttGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAACTTACTTGCTAGAACTTCTGGCGCTAAAGTCTCAGTAt          >  2:8338817/1‑149 (MQ=255)

                                                                                                                                       ttGGACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAACTTACTTGCTAGAACTTCTGGCGCTAAAGTCTCAGTAt          <  2:3570769/149‑1 (MQ=255)

                                                                                                                                         ggACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAACTTACTTGCTAGAACTTCTGGCGCTAAAGTCTCAGTATCa        >  1:3015359/1‑149 (MQ=255)

                                                                                                                                          gACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAACTTACTTGCTAGAACTTCTGGCGCTAAAGTCTCAGTATCaa       >  1:7159365/1‑149 (MQ=255)

                                                                                                                                          gACTTCAAACGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAACTTACTTGCTAGAACTTCTGGCGCTAAAGTCTCAGTATCaa       >  1:5851738/1‑149 (MQ=255)

ATTTGCTTTTAAACTTGCTTTGTTTGCTGCATCTAACACTCTATTACCAGATGTTAGATCTAAAATTTGTTGGTCATATTCGGATTTACGTGTGGTTTCTTGAGAAATTTTTGTTCTCAAATCCATTGTATTCCCTTGGACTTCAAGCGTTTTGGTTTTTACATAAAGTAGTCCAACAGCCAACACTAAAGCAGCAACTACCATCGTCAAGTAGAATACTTTTTCCACACCACTAAATTTTTTAAACTTACTTGCTAGAACTTCTGGCGCTAAAGTCTCAGTATCAATAAGA  >  AM406671/1655634‑1655925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`