BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,681,037	T→C	E332G (GAA→GGA)	pepV ←	Dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,681,037	0	T	C	99.8%	3860.5 / NA	931	E332G (GAA→GGA)	pepV	Dipeptidase
Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (440/485); total (443/488)
Fisher's exact test for biased strand distribution p-value = 2.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 942 total aligned reads displayed.

GTTCAACTTCAACAACCCCATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTTCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGATAAGTTGCTCCATT  >  AM406671/1680891‑1681182

gTTCAACTTCAACAACCCCATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTccc                                                                                                                                                 <  2:7648149/149‑1 (MQ=255)

gTTCAACTTCAACAACCCCATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTccc                                                                                                                                                 <  1:898283/149‑1 (MQ=255)

 ttcaacttcaacAACCCCATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCa                                                                                                                                                <  1:7178788/149‑1 (MQ=255)

 ttcaacttcaacAACCCCATCAAGTTTAGCAAGAATTTCTTGCATAAGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCa                                                                                                                                                >  2:1924347/1‑149 (MQ=255)

     acttcaacAACCCCATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAATTGACCAAACACCAGCATTCATTGCGGTATTTCCCATCaa                                                                                                                                            >  1:2952784/1‑149 (MQ=255)

      cttcaacAACCCCATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAAt                                                                                                                                           <  2:5782697/149‑1 (MQ=255)

       ttcaacAACCCCATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAAtt                                                                                                                                          <  1:8334046/149‑1 (MQ=255)

        tcaacAACCCCATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGCAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATtc                                                                                                                                         >  2:8029203/1‑149 (MQ=255)

        tcaacAACCCCATCAAGTTTAGCAAGAATTTCTTGCATACGCACTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCACTtc                                                                                                                                         >  2:7232838/1‑149 (MQ=255)

         caacaaCCCCATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATtca                                                                                                                                        >  1:2833189/1‑149 (MQ=255)

             aacCCCATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATcaa                                                                                                                                    <  1:650408/149‑1 (MQ=255)

               ccccATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGTTTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATcaaca                                                                                                                                  >  1:5571517/1‑149 (MQ=255)

               ccccATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATcaaca                                                                                                                                  >  1:9126835/1‑149 (MQ=255)

               ccccATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATAAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATcaaca                                                                                                                                  >  1:858788/1‑149 (MQ=255)

               ccccATCAAGTTTAGCAAGAATTTCCTGCATACGCTCTGGGCTGTTCCCATGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATcaaca                                                                                                                                  >  2:5048774/1‑149 (MQ=255)

                cccATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATcaacaa                                                                                                                                 >  1:661920/1‑149 (MQ=255)

                cccATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATcaacaa                                                                                                                                 <  1:4259170/149‑1 (MQ=255)

                 ccATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACaaa                                                                                                                                <  2:6566118/149‑1 (MQ=255)

                  cATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACaaaa                                                                                                                               >  2:1108775/1‑149 (MQ=255)

                    tCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGc                                                                                                                             <  2:7357102/149‑1 (MQ=255)

                           tAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCt                                                                                                                      >  2:3603582/1‑149 (MQ=255)

                               aaGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAAtt                                                                                                                  <  1:6995556/149‑1 (MQ=255)

                               aaGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAAtt                                                                                                                  <  1:5240303/149‑1 (MQ=255)

                                aGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAAttt                                                                                                                 >  1:4092080/1‑149 (MQ=255)

                                  aaTTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAAttttt                                                                                                               >  1:2278095/1‑149 (MQ=255)

                                     ttCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCAc                                                                                                            <  2:9070909/149‑1 (MQ=255)

                                        ttGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCtt                                                                                                         <  1:6293366/149‑1 (MQ=255)

                                        ttGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCtt                                                                                                         <  1:6086543/149‑1 (MQ=255)

                                        ttGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATAGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTCTTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCtt                                                                                                         >  2:6185405/1‑149 (MQ=255)

                                         tGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTc                                                                                                        <  2:1226165/149‑1 (MQ=255)

                                          gCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCa                                                                                                       <  1:4019612/149‑1 (MQ=255)

                                             tACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGa                                                                                                    >  2:4106379/1‑149 (MQ=255)

                                              aCGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGAt                                                                                                   <  2:534924/149‑1 (MQ=255)

                                                  tctGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTc                                                                                               <  1:8445351/149‑1 (MQ=255)

                                                   ctGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCa                                                                                              <  1:3646393/149‑1 (MQ=255)

                                                       gCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCaagaa                                                                                          >  1:6851540/1‑149 (MQ=255)

                                                          gTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGtt                                                                                       <  1:7597935/149‑1 (MQ=255)

                                                           ttCCCTTGTGGGAAACGGAAATTGAGCGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGttt                                                                                      <  2:3319296/149‑1 (MQ=255)

                                                            tCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGtttt                                                                                     >  1:5399634/1‑149 (MQ=255)

                                                             cccTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGttttt                                                                                    <  1:2003310/149‑1 (MQ=255)

                                                              ccTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTc                                                                                   <  2:6362228/149‑1 (MQ=255)

                                                                ttGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAg                                                                                 <  2:5284960/149‑1 (MQ=255)

                                                                  gtgGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGcc                                                                               <  2:8730286/149‑1 (MQ=255)

                                                                     ggAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCa                                                                            >  1:1268376/1‑149 (MQ=255)

                                                                      gAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCaa                                                                           >  2:8463285/1‑149 (MQ=255)

                                                                       aaaCGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAAc                                                                          <  2:1569805/149‑1 (MQ=255)

                                                                        aaCGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACt                                                                         >  2:4608989/1‑149 (MQ=255)

                                                                        aaCGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACt                                                                         <  2:4491177/149‑1 (MQ=255)

                                                                         aCGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACtt                                                                        <  2:7824423/149‑1 (MQ=255)

                                                                            gAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTaa                                                                     <  1:2408257/149‑1 (MQ=255)

                                                                                 tGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCCTTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAg                                                                >  2:2106308/1‑149 (MQ=255)

                                                                                 tGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAg                                                                >  1:8184573/1‑149 (MQ=255)

                                                                                    gggCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGgca                                                             >  2:2694656/1‑149 (MQ=255)

                                                                                     ggCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGgcag                                                            <  1:6748422/149‑1 (MQ=255)

                                                                                      gCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGgcagc                                                           >  1:3813643/1‑149 (MQ=255)

                                                                                        gATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGgcagcag                                                         <  1:3115643/149‑1 (MQ=255)

                                                                                         aTTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGgcagcagc                                                        >  2:3059342/1‑149 (MQ=255)

                                                                                          ttttACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGgcagcagca                                                       <  2:6978657/149‑1 (MQ=255)

                                                                                           tttACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCAc                                                      <  2:292040/149‑1 (MQ=255)

                                                                                             tACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCCGCCCCACCTTTAATGAAGGCAGCAGCACCg                                                    <  2:9238047/149‑1 (MQ=255)

                                                                                              aCCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGt                                                   <  2:7942848/149‑1 (MQ=255)

                                                                                                cTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCa                                                 <  2:8659000/149‑1 (MQ=255)

                                                                                                cTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCa                                                 >  1:7445634/1‑149 (MQ=255)

                                                                                                 ttCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAg                                                <  2:2781002/149‑1 (MQ=255)

                                                                                                   cACCATTTTCATCAAATGACCAACCACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCa                                              <  2:6729953/149‑1 (MQ=255)

                                                                                                   cACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCa                                              <  2:2498995/149‑1 (MQ=255)

                                                                                                   cACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCa                                              <  2:5966246/149‑1 (MQ=255)

                                                                                                    aCCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCaa                                             <  1:1012288/149‑1 (MQ=255)

                                                                                                     ccATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCaaa                                            <  1:4627980/149‑1 (MQ=255)

                                                                                                       aTTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGt                                          >  1:265137/1‑149 (MQ=255)

                                                                                                          ttCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCaa                                       >  2:4276104/1‑149 (MQ=255)

                                                                                                           tcatcaAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCaaa                                      >  2:3179241/1‑149 (MQ=255)

                                                                                                           tcatcaAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCaaa                                      <  1:5880365/149‑1 (MQ=255)

                                                                                                            catcaAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAAt                                     >  2:5593976/1‑149 (MQ=255)

                                                                                                             atcaAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAAtt                                    >  1:5041746/1‑149 (MQ=255)

                                                                                                              tcaAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTg                                   <  2:4748327/149‑1 (MQ=255)

                                                                                                                 aaTGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGAtt                                <  2:826979/149‑1 (MQ=255)

                                                                                                                 aaTGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGAtt                                <  2:8779346/149‑1 (MQ=255)

                                                                                                                    gACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCaa                             >  1:5731158/1‑149 (MQ=255)

                                                                                                                     aCCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAg                            <  1:1101951/149‑1 (MQ=255)

                                                                                                                      ccAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGa                           <  2:5481492/149‑1 (MQ=255)

                                                                                                                      ccAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGa                           <  1:3418901/149‑1 (MQ=255)

                                                                                                                         aaCACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACa                        >  1:1829471/1‑149 (MQ=255)

                                                                                                                         aaCACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACa                        >  2:374104/1‑149 (MQ=255)

                                                                                                                            acCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAg                     <  1:8804828/149‑1 (MQ=255)

                                                                                                                              cAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTa                   <  2:3900480/149‑1 (MQ=255)

                                                                                                                               aGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTaa                  >  2:1386337/1‑149 (MQ=255)

                                                                                                                               aGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTaa                  >  1:8023161/1‑149 (MQ=255)

                                                                                                                                gCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAg                 <  1:480427/149‑1 (MQ=255)

                                                                                                                                 cattcattGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGa                >  2:2353918/1‑149 (MQ=255)

                                                                                                                                  attcattGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGAt               >  1:7909334/1‑149 (MQ=255)

                                                                                                                                   ttcattGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGACTCAAGAACAAAGTAAGATa              >  1:4504566/1‑149 (MQ=255)

                                                                                                                                     cattGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTCATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGATAAg            <  2:176974/149‑1 (MQ=255)

                                                                                                                                     cattGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTTTCCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGATAAg            >  2:7529828/1‑149 (MQ=255)

                                                                                                                                     cattGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGATAAg            >  1:7785539/1‑149 (MQ=255)

                                                                                                                                          aGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGATAAGTTGCt       >  1:1657236/1‑149 (MQ=255)

                                                                                                                                              aTTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGATAAGTTGCTCCAt   >  1:3332066/1‑149 (MQ=255)

                                                                                                                                              aTTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGATAAGTTGCTCCAt   >  1:1429338/1‑149 (MQ=255)

                                                                                                                                              aTTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGATAAGTTGCTCCAt   <  2:8921070/149‑1 (MQ=255)

                                                                                                                                               tttcCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGATAAGTTGCTCCAtt  <  2:8529757/149‑1 (MQ=255)

GTTCAACTTCAACAACCCCATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTTCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGATAAGTTGCTCCATT  >  AM406671/1680891‑1681182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`