BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,817,902	C→A	E463D (GAG→GAT)	xylS ←	alpha‑xylosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,817,902	0	C	A	99.5%	3446.3 / NA	902	E463D (GAG→GAT)	xylS	alpha‑xylosidase
Reads supporting (aligned to +/- strand): ref base C (4/1); new base A (472/424); total (477/425)
Fisher's exact test for biased strand distribution p-value = 3.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 908 total aligned reads displayed.

GGATATTCATAGTAGAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGCTCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTCCACCAAGTATAGCCAA  >  AM406671/1817756‑1818047

  atatTCATAGTAGAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCtt                                                                                                                                               <  2:1349517/149‑1 (MQ=255)

   tatTCATAGTAGAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCttt                                                                                                                                              >  1:157338/1‑149 (MQ=255)

    atTCATAGTAGAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTa                                                                                                                                             >  1:6613376/1‑149 (MQ=255)

     ttCATAGTAGAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTAAATCACCGAATCAAAATGCCAGGGATCTTTAc                                                                                                                                            >  2:2791504/1‑149 (MQ=255)

      tCATAGTAGAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACt                                                                                                                                           >  2:2985597/1‑149 (MQ=255)

       cATAGTAGAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTa                                                                                                                                          >  1:9130516/1‑149 (MQ=255)

         tagtagAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGt                                                                                                                                        >  1:1737267/1‑149 (MQ=255)

         tagtagAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGt                                                                                                                                        <  1:2351424/149‑1 (MQ=255)

           gtagAGTGGTTCACCAAGTGCTCTACCTTCAGAAGCTGTTATTACATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCGTTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGa                                                                                                                                      <  1:5033172/149‑1 (MQ=255)

            tagAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTAc                                                                                                                                            >  1:7802044/1‑142 (MQ=255)

            tagAGTGGTTCAACAAGTCCGCTACCTTCAGAAACTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGaa                                                                                                                                     <  1:2885134/149‑1 (MQ=255)

             agagTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAAt                                                                                                                                    >  2:8076274/1‑149 (MQ=255)

               agTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTc                                                                                                                                  >  2:2585824/1‑149 (MQ=255)

                gTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGCTCTTTACTAGTGAATTCt                                                                                                                                 >  1:7692090/1‑149 (MQ=255)

                gTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCt                                                                                                                                 <  2:6670014/149‑1 (MQ=255)

                 tGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTg                                                                                                                                <  2:4738390/149‑1 (MQ=255)

                 tGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTg                                                                                                                                <  1:1945207/149‑1 (MQ=255)

                 tGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTg                                                                                                                                <  1:2790774/149‑1 (MQ=255)

                       aacaaGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGACTCTTTACTAGTGAATTCTGAtttcg                                                                                                                          >  2:6447926/1‑147 (MQ=255)

                        acaaGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGa                                                                                                                         >  1:4730170/1‑149 (MQ=255)

                         caaGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAg                                                                                                                        >  1:32390/1‑149 (MQ=255)

                           aGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTCGTGAATTCTGATTTTGAGGa                                                                                                                      >  2:8321572/1‑149 (MQ=255)

                              gCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATg                                                                                                                   <  1:6796060/149‑1 (MQ=255)

                               ctctACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGa                                                                                                                  <  1:8719322/149‑1 (MQ=255)

                               ctctACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGa                                                                                                                  >  2:7689209/1‑149 (MQ=255)

                               ctatACCATCAGAAGCTTTTATTAAATTGGCTCTATATAGATATTGAATTCATTGATGACGAAGGTATAAAAAGTCAATCATTGATTGTTCAGTCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGa                                                                                                                  <  2:111069/146‑1 (MQ=255)

                                  tACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGt                                                                                                               >  2:4520031/1‑149 (MQ=255)

                                  tACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGt                                                                                                               >  2:5710907/1‑149 (MQ=255)

                                   aCCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGCATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTc                                                                                                              >  1:9255100/1‑149 (MQ=255)

                                    ccTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCg                                                                                                             <  2:3288037/149‑1 (MQ=255)

                                     cTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCga                                                                                                            <  1:3854413/149‑1 (MQ=255)

                                      ttCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCgat                                                                                                           >  2:897478/1‑149 (MQ=255)

                                       tCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCgatt                                                                                                          >  1:6877441/1‑149 (MQ=255)

                                       tCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCgatt                                                                                                          <  2:6833163/149‑1 (MQ=255)

                                        cAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCgattg                                                                                                         <  1:2667502/149‑1 (MQ=255)

                                        cAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCgattg                                                                                                         <  1:3430319/149‑1 (MQ=255)

                                          gAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCgattgat                                                                                                       >  1:9044264/1‑149 (MQ=255)

                                           aaGCTGGTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGCAATGAAGTCgattgatt                                                                                                      >  2:7569966/1‑149 (MQ=255)

                                               tGTTATTAAATTGGCGCTATAAATATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTg                                                                                                  <  2:6976163/149‑1 (MQ=255)

                                                 ttattaAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGaa                                                                                                <  1:6996932/149‑1 (MQ=255)

                                                  tattaAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGaaa                                                                                               >  2:3531333/1‑149 (MQ=255)

                                                  tattaAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGaaa                                                                                               <  1:417357/149‑1 (MQ=255)

                                                  tattaAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATGGGTGaaa                                                                                               >  2:4080554/1‑149 (MQ=255)

                                                   attaAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGaaaa                                                                                              <  2:5224719/149‑1 (MQ=255)

                                                       aaTTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATAcc                                                                                          <  1:1430793/149‑1 (MQ=255)

                                                       aaTTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATAcc                                                                                          >  1:7254743/1‑149 (MQ=255)

                                                        aTTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATAccc                                                                                         >  1:8111933/1‑149 (MQ=255)

                                                         ttGTCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATGCTGATTTTGAGGAATGAAGTCGATTGATTCGTGAAAATAcccc                                                                                        <  2:1883982/149‑1 (MQ=255)

                                                           ggCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCaa                                                                                      <  2:2286729/149‑1 (MQ=255)

                                                           ggCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCaa                                                                                      <  2:5918885/149‑1 (MQ=255)

                                                             cgcTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAAt                                                                                    <  1:5229203/149‑1 (MQ=255)

                                                              gcTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAAtt                                                                                   <  2:8635494/149‑1 (MQ=255)

                                                                   aaaGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAac                                                                              >  1:795524/1‑149 (MQ=255)

                                                                   aaaGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAac                                                                              >  2:5879293/1‑149 (MQ=255)

                                                                     aGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAacca                                                                            >  1:8428948/1‑149 (MQ=255)

                                                                         atGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCtt                                                                        >  2:6287549/1‑149 (MQ=255)

                                                                         atGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCtt                                                                        >  1:6044723/1‑149 (MQ=255)

                                                                         atGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCtt                                                                        >  2:5428178/1‑149 (MQ=255)

                                                                         atGGAATTAATTGATGACGAAGTTGCAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCtt                                                                        >  2:5001621/1‑149 (MQ=255)

                                                                           ggAATTAATTGATGACGAAGTTGTAAAAAGTCAATGATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTaa                                                                      >  2:1405630/1‑149 (MQ=255)

                                                                           ggAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTaa                                                                      >  1:8279772/1‑149 (MQ=255)

                                                                              attaattGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCga                                                                                                            >  2:9093007/1‑108 (MQ=255)

                                                                              attaattGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACt                                                                   >  2:4870932/1‑149 (MQ=255)

                                                                                 aattGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAg                                                                >  2:7284550/1‑149 (MQ=255)

                                                                                  attGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGc                                                               >  2:3124222/1‑149 (MQ=255)

                                                                                   ttGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTGCTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCt                                                              >  1:5746358/1‑149 (MQ=255)

                                                                                   ttGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCt                                                              >  1:7108172/1‑149 (MQ=255)

                                                                                     gatgaCGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTcc                                                            >  1:8529585/1‑149 (MQ=255)

                                                                                       tgaCGAAGTTGTAAAAAGTCAATCATTGATAGTTCAATCACCGCATCCAAATGCCAGGGATCTTTACTAGTGAATTATGATTTTGAGAAATGAAGTCGATTGATTGTTGAAAATTCCCCCAATATCAACAACCTTAAACTGAGCTcctc                                                          <  2:4302277/149‑3 (MQ=255)

                                                                                         aCGAAGTGGTAAAAAGTCAATCATGGATTGTTCAATCACCGCATCAAAATGCCAGGTATCTTTACTAGTGAATTCTGATTTTCAGGAATGAAGTCGAATGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCAt                                                        <  2:1233562/149‑1 (MQ=255)

                                                                                           gAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCt                                                      <  1:1001389/149‑1 (MQ=255)

                                                                                           gAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATGTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTCAACTGAGCTCCGCATCt                                                      >  1:366214/1‑149 (MQ=255)

                                                                                           gAAGTTGTAAAAAGTCAATCATTGATCGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCt                                                      <  1:5141459/149‑1 (MQ=255)

                                                                                            aaGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCtt                                                     >  1:3990698/1‑149 (MQ=255)

                                                                                            aaGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCtt                                                     <  1:453422/149‑1 (MQ=255)

                                                                                            aaGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCtt                                                     >  2:793637/1‑149 (MQ=255)

                                                                                               ttGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGt                                                  >  1:1255937/1‑149 (MQ=255)

                                                                                               ttGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTCTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGt                                                  <  1:5581329/149‑1 (MQ=255)

                                                                                                   aaaaaGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAAcc                                              <  2:6053953/149‑1 (MQ=255)

                                                                                                   aaaaaGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAAcc                                              <  1:4384022/149‑1 (MQ=255)

                                                                                                   aaaaaGGCAAGCATTGATTGGTCAATCACCGCATCAAAATGCCAGGGATCGTTACTAGTGAATTCTGATTTTGAGGAAGGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAAcc                                              <  1:3046456/149‑1 (MQ=255)

                                                                                                     aaaGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCtt                                            >  1:5866304/1‑149 (MQ=255)

                                                                                                     aaaGTCAATCATGGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTGTGAGGAATGAAGTCGATAGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCtt                                            >  2:165092/1‑149 (MQ=255)

                                                                                                       aGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGc                                          >  2:6569060/1‑149 (MQ=255)

                                                                                                       aGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGc                                          >  1:891710/1‑149 (MQ=255)

                                                                                                        gTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAGCTGAGCTCCGCATCTTTGTAACCTTGCa                                         >  1:6311575/1‑149 (MQ=255)

                                                                                                           aaTCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCAtgt                                      >  1:2164298/1‑149 (MQ=255)

                                                                                                            aTCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCAtgtg                                     >  2:2461188/1‑149 (MQ=255)

                                                                                                             tCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGc                                    >  1:2522428/1‑149 (MQ=255)

                                                                                                              cATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTg                                                                                  >  2:3790551/1‑102 (MQ=255)

                                                                                                                 tgattgTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGccccc                                <  1:635770/149‑1 (MQ=255)

                                                                                                                 tgattgTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGccccc                                >  2:7411564/1‑149 (MQ=255)

                                                                                                                     tgTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAat                            <  1:7551203/149‑1 (MQ=255)

                                                                                                                        tCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATc                         <  2:1502812/149‑1 (MQ=255)

                                                                                                                         cAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCa                        <  2:8899970/149‑1 (MQ=255)

                                                                                                                                      aaaaTGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGCAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTCCACCAAg           >  2:3913964/1‑149 (MQ=255)

                                                                                                                                       aaaTGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTCCACCAAGt          <  2:1962000/149‑1 (MQ=255)

                                                                                                                                       aaaTGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTCCACCAAGt          <  2:2115609/149‑1 (MQ=255)

                                                                                                                                         aTGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTCCACCAAGtat        >  1:5432423/1‑149 (MQ=255)

                                                                                                                                         aTGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTCCACCAAGtat        >  2:9225533/1‑149 (MQ=255)

GGATATTCATAGTAGAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGCTCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTCCACCAAGTATAGCCAA  >  AM406671/1817756‑1818047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`