BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,854,998	T→A	V95V (GTA→GTT)	glgA ←	GlgA protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,854,998	0	T	A	99.8%	4043.6 / NA	1031	V95V (GTA→GTT)	glgA	GlgA protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (494/535); minor base C (2/0); total (496/535)
Fisher's exact test for biased strand distribution p-value = 2.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1033 total aligned reads displayed.

ACATGCAAAATGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTTACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCAACAGAAG  >  AM406671/1854852‑1855143

 cATGCAAAATGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACa                                                                                                                                                <  1:7165131/149‑1 (MQ=255)

  aTGCAAAATGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACAt                                                                                                                                               <  2:5299997/149‑1 (MQ=255)

  aTGCAAAATGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACAt                                                                                                                                               >  2:542632/1‑149 (MQ=255)

   tGCAAAATGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACAtt                                                                                                                                              >  2:3452991/1‑149 (MQ=255)

   tGCAAAATGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACAtt                                                                                                                                              >  1:2966625/1‑149 (MQ=255)

    gCAAAATGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTa                                                                                                                                             >  2:6557576/1‑149 (MQ=255)

    aaaaacTGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCGCGACCAAAATAATCCTCATTATCTAAAAAATAATATTTAACATTa                                                                                                                                             <  2:6281249/147‑1 (MQ=255)

     cAAAATGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTAt                                                                                                                                            <  1:7712524/149‑1 (MQ=255)

        aaTGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCtt                                                                                                                                         <  1:1929092/149‑1 (MQ=255)

        aaTGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCtt                                                                                                                                         <  1:4005471/149‑1 (MQ=255)

           gTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTa                                                                                                                                      <  1:7530274/149‑1 (MQ=255)

           gTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTa                                                                                                                                      >  2:7979936/1‑149 (MQ=255)

            tCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTAc                                                                                                                                     >  1:4190637/1‑149 (MQ=255)

             cAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATTGACAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAGTTAATATTTAACATTATCTTTTACt                                                                                                                                    <  2:5024550/149‑1 (MQ=255)

             cAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACt                                                                                                                                    >  1:4707132/1‑149 (MQ=255)

             cAGGAATAAAATCTAATTTTTCTAAGACCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACt                                                                                                                                    <  2:57342/149‑1 (MQ=255)

              aGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTa                                                                                                                                   <  2:3590659/149‑1 (MQ=255)

               ggAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTaa                                                                                                                                  <  1:6846976/149‑1 (MQ=255)

                gAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGGCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAg                                                                                                                                 <  1:5410396/149‑1 (MQ=255)

                gAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAAACGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCACATAAACTAAAAAATAATATTTAACATTATCTTTTACTAAg                                                                                                                                 <  2:5881520/149‑1 (MQ=255)

                 aaTAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAgg                                                                                                                                <  1:9145196/149‑1 (MQ=255)

                  aTAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGt                                                                                                                               >  2:1814116/1‑149 (MQ=255)

                    aaaaTCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGttt                                                                                                                             >  2:227386/1‑149 (MQ=255)

                     aaaTCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGtttt                                                                                                                            >  2:7774612/1‑149 (MQ=255)

                     aaaTCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGtttt                                                                                                                            >  1:3318261/1‑149 (MQ=255)

                       aTCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTaa                                                                                                                          >  2:6034727/1‑149 (MQ=255)

                        tCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAAc                                                                                                                         >  1:2177450/1‑149 (MQ=255)

                            aTTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCa                                                                                                                     <  1:8484706/149‑1 (MQ=255)

                               tttCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACa                                                                                                                  >  2:1640035/1‑149 (MQ=255)

                               tttCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACa                                                                                                                  <  1:8616910/149‑1 (MQ=255)

                                ttCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACat                                                                                                                 <  1:8840781/149‑1 (MQ=255)

                                 tCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACata                                                                                                                >  1:4414662/1‑149 (MQ=255)

                                 tCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACata                                                                                                                <  1:3605700/149‑1 (MQ=255)

                                   tAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATAtt                                                                                                              >  1:129676/1‑149 (MQ=255)

                                    aaGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTc                                                                                                             <  1:2680544/149‑1 (MQ=255)

                                    aaGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTc                                                                                                             >  2:4952913/1‑149 (MQ=255)

                                    aaGAGCTGACAAACAGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTc                                                                                                             <  2:7069415/149‑1 (MQ=255)

                                       agCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATg                                                                                                          <  1:7817776/149‑1 (MQ=255)

                                          tGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATgccg                                                                                                       <  1:5852849/149‑1 (MQ=255)

                                           gACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTANGGTTTTAACTCCAACATATTCATgccgc                                                                                                      <  1:5092890/149‑1 (MQ=255)

                                           gACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATgccgc                                                                                                      >  2:411761/1‑149 (MQ=255)

                                            aCAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATgccgcc                                                                                                     >  2:8585899/1‑149 (MQ=255)

                                              aaaCGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTATACTAAGGTTTTAACTCCAACATATTCATGCCGCCaa                                                                                                   >  2:8786943/1‑149 (MQ=255)

                                                aCGGCTAAATCGAAAAATGCAAAAAGTTTTCCATCATCACCAAAACCGAACATCATCCCTCGACCATACCTATACTCATTATCTAAAAAATAATATCTAACATCATCTTTTACTAATGTTTTGATTGAAACATATTCTTgccgccttct                                                                                                 <  2:7580519/149‑5 (MQ=255)

                                                aCGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAAcc                                                                                                 <  1:6212865/149‑1 (MQ=255)

                                                 cGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCt                                                                                                <  1:1051570/149‑1 (MQ=255)

                                                  ggCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTa                                                                                               <  1:5664250/149‑1 (MQ=255)

                                                    cTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACa                                                                                             >  1:9230748/1‑149 (MQ=255)

                                                    cTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACa                                                                                             >  1:994498/1‑149 (MQ=255)

                                                       aaTCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATca                                                                                          <  1:8115789/149‑1 (MQ=255)

                                                        aTCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATcaa                                                                                         <  1:9009423/149‑1 (MQ=255)

                                                        aTCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATcaa                                                                                         <  2:7265243/149‑1 (MQ=255)

                                                            aaaaaGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACaaa                                                                                     >  2:1451251/1‑149 (MQ=255)

                                                                aGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCa                                                                                 >  1:8267374/1‑149 (MQ=255)

                                                                aGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCa                                                                                 <  2:431878/149‑1 (MQ=255)

                                                                aGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCa                                                                                 >  1:8956754/1‑149 (MQ=255)

                                                                     aaaCGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATaaaa                                                                            <  1:2311533/149‑1 (MQ=255)

                                                                     aaaCGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATaaaa                                                                            <  1:1510322/149‑1 (MQ=255)

                                                                        cGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTc                                                                         <  2:5565761/149‑1 (MQ=255)

                                                                              ccATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATcgac                                                                   >  1:5239764/1‑149 (MQ=255)

                                                                               catcatCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATcgacg                                                                  >  2:8440329/1‑149 (MQ=255)

                                                                                atcatcACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATcgacga                                                                 <  1:6620439/149‑1 (MQ=255)

                                                                                atcatcACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATcgacga                                                                 >  2:3209898/1‑149 (MQ=255)

                                                                                 tcatcaCCATAACCGTAAATCTGCTCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTCTCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGag                                                                <  2:6734997/149‑1 (MQ=255)

                                                                                 tcatcaCCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGag                                                                >  1:3166489/1‑149 (MQ=255)

                                                                                 tcatcaCCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGag                                                                <  1:378922/149‑1 (MQ=255)

                                                                                      aCCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTg                                                           <  2:2132210/149‑1 (MQ=255)

                                                                                         aTAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAAc                                                        >  2:1362332/1‑149 (MQ=255)

                                                                                         aTAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCAAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCACCCACGAGTAGTGAAc                                                        >  2:3993215/1‑149 (MQ=255)

                                                                                          tAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACg                                                       <  1:2062853/149‑1 (MQ=255)

                                                                                                tAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATAttc                                                 <  2:6934998/149‑1 (MQ=255)

                                                                                                 aaaTCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAAGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATAttct                                                <  2:3320820/149‑1 (MQ=255)

                                                                                                  aaTCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATAttctt                                               <  1:7308042/149‑1 (MQ=255)

                                                                                                     cTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTcttctt                                            >  2:815135/1‑149 (MQ=255)

                                                                                                       gCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCtttc                                          <  1:3450805/149‑1 (MQ=255)

                                                                                                           tCGACCACAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCtttcattt                                      <  2:1186336/149‑1 (MQ=255)

                                                                                                            cGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCtttcatttc                                     >  2:6403914/1‑149 (MQ=255)

                                                                                                                  aaaTAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCAttttt                               <  1:631956/149‑1 (MQ=255)

                                                                                                                  aaaTAATGCCCATTATCTAAAAAATAATATTTAACATTATCTTTTACTACGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAACTTCATCGTCGAGTAGTCAACGAAATTCATTTTTAATTTTAttttt                               >  2:2588094/1‑149 (MQ=255)

                                                                                                                   aataatGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTg                              >  2:2726569/1‑149 (MQ=255)

                                                                                                                   aataatGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTg                              >  1:7947238/1‑149 (MQ=255)

                                                                                                                      aatGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGaaa                           >  2:7384975/1‑149 (MQ=255)

                                                                                                                      aatGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGaaa                           >  1:4901683/1‑149 (MQ=255)

                                                                                                                       atGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCTTAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAAt                          <  2:2980355/149‑1 (MQ=255)

                                                                                                                           tCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAgg                      >  1:4516753/1‑149 (MQ=255)

                                                                                                                            cATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGt                     <  2:8945343/149‑1 (MQ=255)

                                                                                                                             aTTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTa                    >  2:1915708/1‑149 (MQ=255)

                                                                                                                              ttATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTaa                   >  1:604589/1‑149 (MQ=255)

                                                                                                                              ttATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTaa                   >  2:3057571/1‑149 (MQ=255)

                                                                                                                                     aaaaaTAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTCGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATcaca            >  2:8283308/1‑149 (MQ=255)

                                                                                                                                      aaaaTAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCacac           <  2:4481391/149‑1 (MQ=255)

                                                                                                                                       aaaTAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACAcc          >  1:2547528/1‑149 (MQ=255)

                                                                                                                                        aataatATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCa         <  2:4620345/149‑1 (MQ=255)

                                                                                                                                         ataataTTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCaa        <  1:1723369/149‑1 (MQ=255)

                                                                                                                                         ataataTTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCaa        >  1:671720/1‑149 (MQ=255)

                                                                                                                                         ataataTTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCaa        <  1:6468271/149‑1 (MQ=255)

                                                                                                                                          taataTTTAACATTATCTTTTACTTAGGTTTTAACTCCAGCATATTCATGCCGCCAACCTACATCAACGAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCAAc       <  2:9157848/149‑1 (MQ=255)

                                                                                                                                          taataTTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCAAc       >  2:3750013/1‑149 (MQ=255)

                                                                                                                                            atatTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCAACag     >  2:4832874/1‑149 (MQ=255)

                                                                                                                                             tatTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCAACaga    <  2:2408493/149‑1 (MQ=255)

ACATGCAAAATGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTTACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCAACAGAAG  >  AM406671/1854852‑1855143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`