BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	2,266,724	T→C	I10T (ATT→ACT)	argR →	arginine repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	2,266,724	0	T	C	99.7%	4579.3 / NA	1109	I10T (ATT→ACT)	argR	arginine repressor
Reads supporting (aligned to +/- strand): ref base T (1/0); major base C (563/541); minor base A (1/2); total (565/544)
Fisher's exact test for biased strand distribution p-value = 6.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1125 total aligned reads displayed.

ACCATCGATTGCAGCAGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTATTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACGGGACAAAGGAGAG  >  AM406671/2266578‑2266869

acCATCGATTGCAGCAGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAAttacta                                                                                                                                                 <  2:6319863/149‑1 (MQ=255)

 ccATCGATTGCAGCAGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTaa                                                                                                                                                <  2:3564314/149‑1 (MQ=255)

  cATCGATTGCAGCAGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTaaa                                                                                                                                               >  1:8344658/1‑149 (MQ=255)

   aTCGATTGCAGCAGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAg                                                                                                                                              <  2:9156339/149‑1 (MQ=255)

       aTTGCAGCAGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAAt                                                                                                                                          <  2:2533377/149‑1 (MQ=255)

        ttGCAGCAGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAAtt                                                                                                                                         <  2:2369892/149‑1 (MQ=255)

        ttGCAGCAGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAACTATACAGAAAAACCACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAAtt                                                                                                                                         >  2:523747/1‑149 (MQ=255)

             gcagACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGAc                                                                                                                                    >  2:7998072/1‑149 (MQ=255)

              cagACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGCAAAAACACTAATTTTGTCAATATATAGTCCATTATAACGTATAATTCTTCTATGAAAAGAGACAAAAGATTAGAAATTACTAAAGAAATTGTGACa                                                                                                                                   >  2:8334622/1‑149 (MQ=255)

               agACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACaa                                                                                                                                  >  1:3767495/1‑149 (MQ=255)

                gACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACaaa                                                                                                                                 >  1:8146870/1‑149 (MQ=255)

                 aCAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAaat                                                                                                                                <  2:2990933/149‑1 (MQ=255)

                      gCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAAtaataa                                                                                                                           >  2:4751734/1‑149 (MQ=255)

                           gCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAatat                                                                                                                      <  1:1843288/149‑1 (MQ=255)

                            cTTACTAGTTGTTTTTCGTCCAGTCACTAATTATACAGAAACAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATAtt                                                                                                                     <  1:288528/149‑1 (MQ=255)

                               aCTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAACGAAATTGTGACAAATAATAAAATATTGAc                                                                                                                  <  2:5668846/149‑1 (MQ=255)

                                  aGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCa                                                                                                               <  2:5500156/149‑1 (MQ=255)

                                  aGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCa                                                                                                               >  1:4927987/1‑149 (MQ=255)

                                   gttgttTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCaa                                                                                                              <  2:720532/149‑1 (MQ=255)

                                     tgttTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCaaga                                                                                                            <  1:1125271/149‑1 (MQ=255)

                                     tgttTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCaaga                                                                                                            <  1:5961435/149‑1 (MQ=255)

                                      gttTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCaagaa                                                                                                           >  2:1005132/1‑149 (MQ=255)

                                        ttttCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAagaaga                                                                                                         <  1:849867/149‑1 (MQ=255)

                                         tttCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAagag                                                                                                        >  2:5847228/1‑149 (MQ=255)

                                          ttCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGc                                                                                                       >  2:8950121/1‑149 (MQ=255)

                                            cGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCtt                                                                                                     <  1:1412619/149‑1 (MQ=255)

                                             gTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTc                                                                                                    <  2:3173467/149‑1 (MQ=255)

                                               ccATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCaa                                                                                                  >  1:608815/1‑149 (MQ=255)

                                                cATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAGAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAAt                                                                                                 <  1:5791233/149‑1 (MQ=255)

                                                cATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAAt                                                                                                 >  1:3578153/1‑149 (MQ=255)

                                                cATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAAt                                                                                                 <  1:555808/149‑1 (MQ=255)

                                                 aTTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAAta                                                                                                >  2:4253868/1‑148 (MQ=255)

                                                    cACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATctct                                                                                             >  2:2197924/1‑149 (MQ=255)

                                                       tAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCtatt                                                                                          >  1:4759377/1‑149 (MQ=255)

                                                         aTTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCtattat                                                                                        >  1:1567327/1‑149 (MQ=255)

                                                          ttATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTattatt                                                                                       >  1:1734729/1‑149 (MQ=255)

                                                          ttATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTattatt                                                                                       <  1:3198888/149‑1 (MQ=255)

                                                          ttATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTattatt                                                                                       >  2:7830482/1‑149 (MQ=255)

                                                             taCAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGa                                                                                    <  1:8788400/149‑1 (MQ=255)

                                                                aGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAAcg                                                                                 >  1:3405124/1‑149 (MQ=255)

                                                                  aaaaaaCACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAAcgcg                                                                               >  2:8824075/1‑149 (MQ=255)

                                                                  aaaaaaCACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAAcgcg                                                                               >  2:2665136/1‑149 (MQ=255)

                                                                  aaaaaaCACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAAcgcg                                                                               <  1:3852361/149‑1 (MQ=255)

                                                                   aaaaaCACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCgg                                                                              >  2:5351531/1‑149 (MQ=255)

                                                                     aaaCACTAATTTTGTGAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGgtg                                                                            >  2:5074037/1‑149 (MQ=255)

                                                                     aaaCACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGgtg                                                                            >  1:6578109/1‑149 (MQ=255)

                                                                     aaaCACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGgtg                                                                            <  2:1904161/149‑1 (MQ=255)

                                                                      aaCACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGgtgt                                                                           >  1:1292665/1‑149 (MQ=255)

                                                                        cacTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTag                                                                         >  2:2072125/1‑149 (MQ=255)

                                                                         acTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTaga                                                                        >  2:1455117/1‑149 (MQ=255)

                                                                          cTAATTTTGTCAATATATAGTCCATTCTATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCNATCTCTATTATTAGAACGCGGTGTagag                                                                       <  1:5112606/149‑1 (MQ=255)

                                                                          cTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTagag                                                                       <  2:2938237/149‑1 (MQ=255)

                                                                           tAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAgg                                                                      >  1:6326460/1‑149 (MQ=255)

                                                                           tAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAgg                                                                      >  2:4075901/1‑149 (MQ=255)

                                                                              ttttGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTaa                                                                   <  2:7603232/149‑1 (MQ=255)

                                                                                 tGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAacac                                                                >  2:908433/1‑149 (MQ=255)

                                                                                  gTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAAcaca                                                               <  1:4125416/149‑1 (MQ=255)

                                                                                  gTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAAcaca                                                               <  2:6525145/149‑1 (MQ=255)

                                                                                  gTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAAcaca                                                               >  1:4030342/1‑149 (MQ=255)

                                                                                    cAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAg                                                             >  2:1163442/1‑149 (MQ=255)

                                                                                    cAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAg                                                             >  1:7208030/1‑149 (MQ=255)

                                                                                      atatatAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGcc                                                           >  2:7430760/1‑149 (MQ=255)

                                                                                       tatataGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTAAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCa                                                          >  1:3709024/1‑149 (MQ=255)

                                                                                        atataGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAAcaca                                                               <  1:1018190/143‑1 (MQ=255)

                                                                                           taGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACttt                                                      >  2:3389193/1‑149 (MQ=255)

                                                                                            aGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTa                                                     >  1:7077082/1‑149 (MQ=255)

                                                                                            aGTCCATTATATGGAATAATTAAGCTATGAAAAGGCATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAAACTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTa                                                     <  2:1311830/149‑1 (MQ=255)

                                                                                              tCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATc                                                   >  1:2595710/1‑149 (MQ=255)

                                                                                               cgaTTATATGGTATAATTATGCTATGAAAAGAGATAAAAGCTTTGAAATTACTAAAGAAATCGTGACAAATAACAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATcc                                                  <  2:6639811/147‑1 (MQ=255)

                                                                                                 aTTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCag                                                <  1:7398025/149‑1 (MQ=255)

                                                                                                  ttATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCaga                                               <  2:454045/149‑1 (MQ=255)

                                                                                                   tataTGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCagag                                              <  1:7831453/149‑1 (MQ=255)

                                                                                                   tataTGGTATAATTATGCGATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCCCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCagag                                              <  1:4258664/149‑1 (MQ=255)

                                                                                                    atatGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAgaga                                             >  1:5406547/1‑149 (MQ=255)

                                                                                                     tatGGTATAATTATGTTATGAAAAGAGCTAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTCTATCCatagtt                                            >  2:2810995/1‑147 (MQ=255)

                                                                                                       tGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGatat                                          <  2:4927745/149‑1 (MQ=255)

                                                                                                       tGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGatat                                          <  2:3831672/149‑1 (MQ=255)

                                                                                                        ggTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTCACACAAGCCACTTTATCCAGAGATAtt                                         <  1:9063385/149‑1 (MQ=255)

                                                                                                        ggTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATTCAGAGATAtt                                         >  1:2034258/1‑149 (MQ=255)

                                                                                                        ggTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATAtt                                         <  1:4633094/149‑1 (MQ=255)

                                                                                                         gTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATACAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTa                                        <  1:7975396/149‑1 (MQ=255)

                                                                                                            taATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGaa                                     >  2:6998524/1‑149 (MQ=255)

                                                                                                             aaTTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGaaa                                    >  1:226952/1‑149 (MQ=255)

                                                                                                             aaTTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGaaa                                    >  2:365956/1‑149 (MQ=255)

                                                                                                              aTTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGaaaa                                   >  1:7764532/1‑149 (MQ=255)

                                                                                                               ttATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAAt                                  >  1:3039085/1‑149 (MQ=255)

                                                                                                                 aTGCTGTGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTa                                <  1:5240265/149‑1 (MQ=255)

                                                                                                                 aTGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTa                                >  1:689662/1‑149 (MQ=255)

                                                                                                                  tGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTaa                               >  1:7598356/1‑149 (MQ=255)

                                                                                                                    cTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAat                             <  1:6760548/149‑1 (MQ=255)

                                                                                                                      aTGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAatat                           <  2:6826883/149‑1 (MQ=255)

                                                                                                                      aTGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAatat                           >  2:6024922/1‑149 (MQ=255)

                                                                                                                               gaTAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTataaataa                  >  2:182947/1‑149 (MQ=255)

                                                                                                                                   aaaGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACgg              <  2:1690249/149‑1 (MQ=255)

                                                                                                                                    aaGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACggg             <  2:8146870/149‑1 (MQ=255)

                                                                                                                                       aTTAGAAATTACTAAAGAAATTGTGACAAATAATAAGATATTGACTCAAGAAGAGATTCAATCACTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACGGGACa          >  2:6165248/1‑149 (MQ=255)

                                                                                                                                          aGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACGGGACAAAg       <  1:2450418/149‑1 (MQ=255)

                                                                                                                                            aaaTTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACGGGACAAAGga     <  2:4154908/149‑1 (MQ=255)

                                                                                                                                            aaaTTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACGGGACAAAGga     <  2:8439274/149‑1 (MQ=255)

                                                                                                                                             aaTTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTGTATCCAGAGATATTAGAAAATTAAATATTATGAAAAAGCGGGACAAAGgag    >  1:4493114/1‑149 (MQ=255)

ACCATCGATTGCAGCAGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTATTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACGGGACAAAGGAGAG  >  AM406671/2266578‑2266869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`