BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,933,214	2 bp→CG	coding (224‑225/714 nt)	atpB ←	ATP synthase F0, A subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,933,214	0	G	C	99.9%	3807.2 / NA	912	G75A (GGC→GCG)	atpB	ATP synthase F0, A subunit
Reads supporting (aligned to +/- strand): ref base G (0/1); new base C (473/437); total (474/438)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	AM406671	1,933,215	0	C	G	99.7%	3823.9 / NA	913	G75A (GGC→GCG)	atpB	ATP synthase F0, A subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (474/436); minor base A (1/2); total (475/438)
Fisher's exact test for biased strand distribution p-value = 6.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 913 total aligned reads displayed.

ACAAGAAGTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGGCCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGAAAACAA  >  AM406671/1933071‑1933356

||

accagaagTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCaaa                                                                                                                                           >  2:1513913/4‑149 (MQ=255)

     aagTGCAAGTGTCAACTAGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTGACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCgtg                                                                                                                                      <  2:4448957/149‑1 (MQ=255)

     aagTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCgtg                                                                                                                                      <  1:1246311/149‑1 (MQ=255)

       gTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCgtggt                                                                                                                                    <  2:8691018/149‑1 (MQ=255)

       gTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCgtggt                                                                                                                                    >  1:2285226/1‑149 (MQ=255)

          cAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCt                                                                                                                                 >  2:4026879/1‑149 (MQ=255)

          cAAGTGTCAAAGCGACTGATGGACTTGCAGTTGGTGATTTCCAAAGAGAAACATCGCCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCt                                                                                                                                 <  2:3744527/149‑1 (MQ=255)

             gtgtCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCa                                                                                                                              <  1:6909224/149‑1 (MQ=255)

             gtgtCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCa                                                                                                                              <  2:7415335/149‑1 (MQ=255)

             gtgtCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCa                                                                                                                              >  1:7040891/1‑149 (MQ=255)

              tgtCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCAt                                                                                                                             >  1:352564/1‑149 (MQ=255)

              tgtCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCAt                                                                                                                             >  2:7803041/1‑149 (MQ=255)

               gtCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATg                                                                                                                            <  2:3552435/149‑1 (MQ=255)

                 cAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAg                                                                                                                          <  1:3033994/149‑1 (MQ=255)

                     tCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGcc                                                                                                                      <  2:7885098/149‑1 (MQ=255)

                     tCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATCAGCGCAAAGCGTGGTGCTTCATGAGGGcc                                                                                                                      >  2:5395997/1‑149 (MQ=255)

                      cGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCg                                                                                                                     <  1:7402379/149‑1 (MQ=255)

                      cGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCg                                                                                                                     >  2:6032655/1‑149 (MQ=255)

                        aCTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGAc                                                                                                                   >  1:5378482/1‑149 (MQ=255)

                             tGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTAt                                                                                                              <  1:5207345/149‑1 (MQ=255)

                                 tttGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCttt                                                                                                          <  1:4195059/149‑1 (MQ=255)

                                 tttGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCttt                                                                                                          >  1:807057/1‑149 (MQ=255)

                                      aGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGaaa                                                                                                     <  2:4911548/149‑1 (MQ=255)

                                        ttGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACt                                                                                                   >  1:6483022/1‑149 (MQ=255)

                                        ttGGTGAGTCCCGAACAGAAACATCACCTGACCTACTAATTTTAGTTCCTAGCCCAATGCAGTTAGCAATAAGAAGTAAAGAAAAGGTGGTAAAGTTCATGAGCACAAAGCGTGGTGCTTCATGAGTGCCGACGTTATCTTTGGAAACt                                                                                                   <  2:728951/149‑1 (MQ=255)

                                          ggTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTcc                                                                                                 <  2:475931/149‑1 (MQ=255)

                                           gTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCa                                                                                                <  1:1378472/149‑1 (MQ=255)

                                            tGAGTTCCGAAGAGAAGAATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGACCGCAAAGCGTGTTGCTTCATGAGGGCCGACGCTATCTTTGGAAACTCCAt                                                                                               <  2:587731/149‑1 (MQ=255)

                                             gATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGCTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTGATCTTTGGAAACTCAAtt                                                                                              >  2:6371061/1‑149 (MQ=255)

                                              atctCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTg                                                                                             <  1:6147426/146‑1 (MQ=255)

                                                ttCCAAAGAGAAACATAACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGAc                                                                                           <  2:1267080/149‑1 (MQ=255)

                                                tgccAAAGAGAAACATCACCTGGCGTACTGATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGCAAGAAAAGAGGGCAAAGTTCATGAGCGCAAAGCGTGGGTCTTCACGAGGGCAGACGTTACCTTGGGAAACCCCATTGAc                                                                                           >  2:1016573/3‑149 (MQ=255)

                                                   cAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACaaa                                                                                        <  1:1007259/149‑1 (MQ=255)

                                                   cAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACaaa                                                                                        <  1:5674420/149‑1 (MQ=255)

                                                    aaaGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACaaaa                                                                                       >  2:2981502/1‑149 (MQ=255)

                                                    aaaGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACaaaa                                                                                       <  1:1782719/149‑1 (MQ=255)

                                                    aaaGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACaaaa                                                                                       >  1:8330339/1‑149 (MQ=255)

                                                     aaGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAAt                                                                                      >  1:275344/1‑149 (MQ=255)

                                                      agagAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATc                                                                                     <  1:475006/149‑1 (MQ=255)

                                                          aaaCATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATcaaca                                                                                 >  1:3236578/1‑149 (MQ=255)

                                                           aaCATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATcaacaa                                                                                <  1:506113/149‑1 (MQ=255)

                                                              aTCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAAccc                                                                             <  1:3328997/149‑1 (MQ=255)

                                                               tccccTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCa                                                                            <  1:5751237/146‑1 (MQ=255)

                                                                cACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCAc                                                                           >  2:2029272/1‑149 (MQ=255)

                                                                   cTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCa                                                                        >  1:399400/1‑149 (MQ=255)

                                                                    tGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGAGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCaa                                                                       <  2:6118893/149‑1 (MQ=255)

                                                                     ggCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAg                                                                      <  1:2048419/149‑1 (MQ=255)

                                                                     ggCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAg                                                                      >  1:4520221/1‑149 (MQ=255)

                                                                      gCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGa                                                                     <  2:8829662/149‑1 (MQ=255)

                                                                       cGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGaa                                                                    >  1:7946195/1‑149 (MQ=255)

                                                                       cGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGaa                                                                    <  1:7495235/149‑1 (MQ=255)

                                                                        gTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAAc                                                                   >  1:8172626/1‑149 (MQ=255)

                                                                          aCTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACAt                                                                 <  2:8238104/149‑1 (MQ=255)

                                                                          aCTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACAt                                                                 <  2:314753/149‑1 (MQ=255)

                                                                          aCTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACAt                                                                 <  2:5236013/149‑1 (MQ=255)

                                                                          aCTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACAt                                                                 <  1:9117901/149‑1 (MQ=255)

                                                                           cTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACAtt                                                                >  1:6258206/1‑149 (MQ=255)

                                                                                tttAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGtt                                                           <  2:2331620/149‑1 (MQ=255)

                                                                                 ttAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGGAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGttt                                                          <  2:3909879/149‑1 (MQ=255)

                                                                                  tAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTa                                                         <  2:908726/149‑1 (MQ=255)

                                                                                       aCTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCttt                                                    >  1:5562339/1‑149 (MQ=255)

                                                                                       aCTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCttt                                                    >  1:7249131/1‑149 (MQ=255)

                                                                                        cTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTg                                                   <  1:2462186/149‑1 (MQ=255)

                                                                                        cTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTg                                                   <  2:5066225/149‑1 (MQ=255)

                                                                                         tAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTgg                                                  >  2:5625143/1‑149 (MQ=255)

                                                                                           gCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGc                                                >  1:4608576/1‑149 (MQ=255)

                                                                                           gCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGc                                                >  2:6600335/1‑149 (MQ=255)

                                                                                           gCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGc                                                <  1:3829233/149‑1 (MQ=255)

                                                                                             ccAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCtt                                              <  2:3296282/149‑1 (MQ=255)

                                                                                             ccAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCtt                                              >  1:1659403/1‑149 (MQ=255)

                                                                                              cAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTg                                             <  2:272683/149‑1 (MQ=255)

                                                                                              cAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTg                                             <  2:6721980/149‑1 (MQ=255)

                                                                                                aTGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAg                                           >  2:4253033/1‑149 (MQ=255)

                                                                                                      ttGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCat                                     <  1:4380351/149‑1 (MQ=255)

                                                                                                      ttGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCat                                     >  2:8097490/1‑149 (MQ=255)

                                                                                                          cAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTg                                 >  1:2851759/1‑149 (MQ=255)

                                                                                                           aaTAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGc                                >  1:5933720/1‑149 (MQ=255)

                                                                                                              aagaagGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGc                             >  1:8761838/1‑149 (MQ=255)

                                                                                                                 aagGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAAccc                                                                             >  2:9235477/1‑98 (MQ=255)

                                                                                                                 aagGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAAccc                                                                             <  1:9235477/98‑1 (MQ=255)

                                                                                                                 aagGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCg                          >  2:2611468/1‑149 (MQ=255)

                                                                                                                 aagGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGAGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCg                          >  1:2951196/1‑149 (MQ=255)

                                                                                                                  agGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGc                         >  2:7628525/1‑149 (MQ=255)

                                                                                                                  agGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGc                         >  1:3647081/1‑149 (MQ=255)

                                                                                                                     aaagaaaagaGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCa                      >  2:1848533/1‑149 (MQ=255)

                                                                                                                     aaagaaaagaGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCa                      >  1:7722656/1‑149 (MQ=255)

                                                                                                                         aaaagaGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAAt                  >  2:8438714/1‑149 (MQ=255)

                                                                                                                          aaagaGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGGTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTGGCCAACAATa                 >  2:6914052/1‑149 (MQ=255)

                                                                                                                            agagGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATAcc               >  1:1528365/1‑149 (MQ=255)

                                                                                                                            agagGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATAcc               >  1:4921084/1‑149 (MQ=255)

                                                                                                                            agagGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCGTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATAcc               >  1:6678865/1‑149 (MQ=255)

                                                                                                                               gggTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGa            >  1:8521757/1‑149 (MQ=255)

                                                                                                                                ggTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCCGAc           >  2:8004385/1‑149 (MQ=255)

                                                                                                                                  tAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCg         >  2:4530447/1‑149 (MQ=255)

                                                                                                                                   aaaGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGTCGACGTTATCTTTGGAAACTCCATTGTCAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGa        <  2:5122359/149‑1 (MQ=255)

                                                                                                                                    aaGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGaa       >  2:1681474/1‑149 (MQ=255)

                                                                                                                                     aGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGaaa      >  2:2516775/1‑149 (MQ=255)

                                                                                                                                        tCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGAAAACa   >  2:7121161/1‑149 (MQ=255)

                                                                                                                                        tCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGAAAACa   >  1:8944055/1‑149 (MQ=255)

                                                                                                                                        tCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGAAAACa   >  1:2655962/1‑149 (MQ=255)

||

ACAAGAAGTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGGCCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGAAAACAA  >  AM406671/1933071‑1933356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`